mip-1α Search Results


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  • 95
    Thermo Fisher macrophage inflammatory protein mip 1 α
    DCEO decreased mRNA levels of cytokines and chemokines in LPS-stimulated RAW264.7 cells. Notes: Cells were incubated with indicated concentrations of DCEO for 1 h and then stimulated with LPS for 6 h. The mRNA levels of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), MCP-1 ( D ), CCL-5 ( E ), and <t>MIP-1α</t> ( F ) were quantified by qRT-PCR. Data presented in bar charts are mean±SEM values from three independent experiments. ** P
    Macrophage Inflammatory Protein Mip 1 α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore mip 1α
    Role of <t>MIP-1α</t> and CCR5 in Aβ 1–40 -induced synaptic disruption. A : Representative images of synaptophysin immunostaining in the CA1 subregion of the hippocampus evaluated 8 days after PBS, Aβ 1–40 , or Aβ 40–1 i.c.v. administration (400 pmol/mouse). Original magnification, ×40. B : Graphic representation of the average optical density (O.D.) of the immunostaining for synaptophysin evaluated in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus, demonstrating that MIP-1α −/− and CCR5 −/− mice were significantly more resistant than wild-type mice to Aβ 1–40 -induced synaptic disruption. The values represent the mean ± SEM ( N = 5 mice/group). ** P
    Mip 1α, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad mip 1α
    <t>MIP-1α</t> and MIP-1β expression in LPS-primed neonatal and adult monocytes following co-stimulation with Ureaplasma isolates. Relative quantification of chemokine mRNA (A, B) and concentrations of secreted protein (C, D) are presented as mean ± SD ( ○ p
    Mip 1α, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson mip 1α
    Functional characteristics of LentiVIP-DC. ( a ) Untransduced-, LentiGFP-, and LentiVIP-DC were incubated in medium containing 1 mg/ml of dextran-PE (40 kd) for 2 hours at 4 °C (control; open histograms) or 37 °C (filled histograms), extensively washed and analyzed by flow cytometry. One representative experiment of two performed in duplicate is shown. ( b ) Untransduced-, LentiGFP-, and LentiVIP-DC were cultured with LPS (1 µg/ml) for 24 hours. Culture supernatants were assayed for IL-12p70, IL-1β, TNF-α, IL-6, <t>MIP-1α,</t> and IL-10. Results are the mean ± SD of three experiments performed in duplicate. Degree of significance P
    Mip 1α, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PeproTech mip 1α
    <t>MIP-1α</t> ( a ) and MCP-1 ( b ) production by mouse PMN after 2 and 24 h stimulation with bacterial filtrates obtained from planktonic (PL) or biofilm (B) cultures of S. aureus strains (8325-4, Wood 46, Sa-α11, Sa-α21. c Unstimulated control cells. All data are the means ± SD. *A significant differences ( p
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    92
    Thermo Fisher mip 1α
    Ex vivo cytokines production upon TLR7 or TLR8 agonist stimulation during whole blood culture. Fresh whole blood specimens from male volunteers were stimulated with TLR7 agonist (3M-001) (A–E) or TLR8 agonist (3M-002) (F–J) for 12 hr. The concentrations of IFN-α, IL-1β, IL-6, TNF-α, and <t>MIP-1α</t> concentrations in the supernatant were measured by high sensitive IFN-α ELISA and Luminex100 system. Each dot represents an individual, (•) represent individuals with TLR7 IVS2-151A/TLR8 -129G genotype, (▪) represent individuals with TLR7 IVS2-151G/TLR8 -129G genotype and (▴) represent those with TLR7 IVS2-151A/TLR8 -129C genotype. The horizontal bars represent mean value.
    Mip 1α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad macrophage inflammatory protein mip 1 α
    Ex vivo cytokines production upon TLR7 or TLR8 agonist stimulation during whole blood culture. Fresh whole blood specimens from male volunteers were stimulated with TLR7 agonist (3M-001) (A–E) or TLR8 agonist (3M-002) (F–J) for 12 hr. The concentrations of IFN-α, IL-1β, IL-6, TNF-α, and <t>MIP-1α</t> concentrations in the supernatant were measured by high sensitive IFN-α ELISA and Luminex100 system. Each dot represents an individual, (•) represent individuals with TLR7 IVS2-151A/TLR8 -129G genotype, (▪) represent individuals with TLR7 IVS2-151G/TLR8 -129G genotype and (▴) represent those with TLR7 IVS2-151A/TLR8 -129C genotype. The horizontal bars represent mean value.
    Macrophage Inflammatory Protein Mip 1 α, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam mip 1α
    Chemokine levels in lung tissue. N = 7/group. LPS injection increased levels of <t>MIP-1α</t> (A, p
    Mip 1α, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher anti mip 1α pe
    Characteristic of microglial cells treated with DHE using a flow cytometer. (A) Dot plot of microglia to gate CD11b-positive and CD206-positive cells treated either with or without DHE. (B) Ratio (%) of CD11b-positive and CD206-positive cells to CD11b-single positive cells. (C-F) Median fluorescent intensity of I-A/I-E, CD86, CD80 and CD68 in CD11b-positive cells. (G-J) Median fluorescent intensity of I-A/I-E, CD86, CD80 and CD68 in CD11b-positive and CD206-positive cells. K-N. Ratio (%) of TNF-α, IL-1β, <t>MIP-1αin</t> IL-12p40/p70-positive to CD11b-positive cells, respectively. O-R. Ratio (%) of TNF-α, IL-1β, <t>MIP-1α</t> and IL-12p40/p70-positive in CD11b-positive and CD206-positive cells, respectively.
    Anti Mip 1α Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson macrophage inflammatory protein mip 1 α
    CyaA-induced cytokine and chemokine production is altered in TLR4-defective mice. Bone marrow derived DC from C3H/HeN or C3H/HeJ mice (10 6 /ml) were cultured with the indicated concentrations of LPS (0 to 10 ng/ml), CyaA (1 μg/ml), in the presence or absence of polymyxin B (PB) (10 μg/ml). Supernatants were tested by immunoassay for IL-10 and <t>MIP-1α</t> (4 h) and IL-12p70 and TNF-α (24 h). Results are means (error bars, SD) of triplicate assays and are representative of three experiments. Symbols: **, P
    Macrophage Inflammatory Protein Mip 1 α, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    R&D Systems mouse ccl3 mip 1 alpha antibody
    CyaA-induced cytokine and chemokine production is altered in TLR4-defective mice. Bone marrow derived DC from C3H/HeN or C3H/HeJ mice (10 6 /ml) were cultured with the indicated concentrations of LPS (0 to 10 ng/ml), CyaA (1 μg/ml), in the presence or absence of polymyxin B (PB) (10 μg/ml). Supernatants were tested by immunoassay for IL-10 and <t>MIP-1α</t> (4 h) and IL-12p70 and TNF-α (24 h). Results are means (error bars, SD) of triplicate assays and are representative of three experiments. Symbols: **, P
    Mouse Ccl3 Mip 1 Alpha Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pharmingen mip 1α
    Relative variation in β-chemokine production by HIV-1-infected CD4 + T cells versus the uninfected controls. The differences among the levels of <t>MIP-1α,</t> MIP-1β, and RANTES produced by CD4 + T cells infected with several viral isolates (NSI, SV [○], NB [□], and LSP [▵]; SI, KP [▴], EM [■], and SF2 [•]) and their concurrent control cultures (uninfected cells from the same donors) are shown. Cells from seven different donors were used. Purified CD4 + T cells were stimulated for 3 days with 3 μg of PHA per ml, washed, and subsequently infected with various NSI and SI virus strains and cultured as described in Materials and Methods. Each symbol represents results with the virus in a separate experiment. The concentration of chemokines in the cell culture fluids was measured by ELISA in duplicate (Quantikine; R D Systems). The levels of chemokines measured in culture fluids from day 13 are shown (one measurement from day 11 of cells plated at 10 6 on day 8 is included) in reference to the level in the control cultures. Control level ranges (picograms per milliliter): MIP-1α, 58 to 4,261; MIP-1β, 46 to 1,117; RANTES, undetectable to 38. All results are adjusted per 10 6 viable cells. Cell numbers (10 6 ) on day 13: control ( n = 6), 1.2 ± 0.21; NSI ( n = 8), 0.93 ± 0.24; SI ( n = 9), 0.67 ± 0.17. Compared to control cultures, NSI viruses increased MIP-1α and MIP-1β production significantly ( P
    Mip 1α, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology mip 1α
    Effect of TGF-β1 on IL-8 and <t>MIP-1α</t> expression. (a) IL-8 mRNA expression was analysed in RA, OA and non-arthritic FLS treated with TGF-β1 for 2 or 4 h. RT-PCR was performed as described in Materials and methods. (b) IL-8 protein expression was analysed by immunoblotting using anti-IL-8 antibody. RA5 FLS were stimulated with TGF-β and/or TNFα for 12h in the absence (lanes 1–4) or presence (lanes 5–8) of BFA (0·5 μg/ml). (c) MIP-1α mRNA expression was analysed by RT-PCR as in (a).
    Mip 1α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Abcam macrophage inflammatory protein mip 1α
    Effect of TGF-β1 on IL-8 and <t>MIP-1α</t> expression. (a) IL-8 mRNA expression was analysed in RA, OA and non-arthritic FLS treated with TGF-β1 for 2 or 4 h. RT-PCR was performed as described in Materials and methods. (b) IL-8 protein expression was analysed by immunoblotting using anti-IL-8 antibody. RA5 FLS were stimulated with TGF-β and/or TNFα for 12h in the absence (lanes 1–4) or presence (lanes 5–8) of BFA (0·5 μg/ml). (c) MIP-1α mRNA expression was analysed by RT-PCR as in (a).
    Macrophage Inflammatory Protein Mip 1α, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore macrophage inflammatory protein mip 1α
    Preinfection genital inflammation was associated with acquisition of less infectious human immunodeficiency virus (HIV). A , Unsupervised hierarchical clustering was used to visualize the variation in cytokine concentrations in individual women and to cluster women according to the similarities of their cytokine expression profiles (using Qlucore Omics Explorer software, version 3.0). Women from whom less infectious viruses were isolated (value below median for relative light units per picogram of reverse-transcriptase [RLUs/pg RT]; n = 13; green blocks ) had up-regulated cytokine concentrations in preinfection cervicovaginal lavage (CVL) samples and tended to cluster together. Women from whom highly infectious viruses were isolated (RLUs/pg RT at or above the median) had lower cytokine concentrations and also clustered together (n = 14; yellow blocks ). Cytokine concentrations are indicated using a color scale, ranging from blue ( low ) to red ( high ). The dendrogram above the heat map illustrates degrees of relatedness between genital cytokine profiles evident among the various women. Patient identity numbers are indicated below the heat map. B , RLUs/pg RT and cytokine concentrations were either log 10 -transformed or converted to categorical variables <t>(MIP-1α,</t> MIP-1β, GM-CSF, and IL-7), and β coefficients were calculated using linear regression. Factor scores for each functional group of cytokines were generated using confirmatory factor analysis. Circles indicate β coefficients from regression analyses; error bars, 95% confidence intervals. Black circles indicate associations between cytokine factors and RLUs/pg RT that were statistically significant (unadjusted P
    Macrophage Inflammatory Protein Mip 1α, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PeproTech elisa kits
    Preinfection genital inflammation was associated with acquisition of less infectious human immunodeficiency virus (HIV). A , Unsupervised hierarchical clustering was used to visualize the variation in cytokine concentrations in individual women and to cluster women according to the similarities of their cytokine expression profiles (using Qlucore Omics Explorer software, version 3.0). Women from whom less infectious viruses were isolated (value below median for relative light units per picogram of reverse-transcriptase [RLUs/pg RT]; n = 13; green blocks ) had up-regulated cytokine concentrations in preinfection cervicovaginal lavage (CVL) samples and tended to cluster together. Women from whom highly infectious viruses were isolated (RLUs/pg RT at or above the median) had lower cytokine concentrations and also clustered together (n = 14; yellow blocks ). Cytokine concentrations are indicated using a color scale, ranging from blue ( low ) to red ( high ). The dendrogram above the heat map illustrates degrees of relatedness between genital cytokine profiles evident among the various women. Patient identity numbers are indicated below the heat map. B , RLUs/pg RT and cytokine concentrations were either log 10 -transformed or converted to categorical variables <t>(MIP-1α,</t> MIP-1β, GM-CSF, and IL-7), and β coefficients were calculated using linear regression. Factor scores for each functional group of cytokines were generated using confirmatory factor analysis. Circles indicate β coefficients from regression analyses; error bars, 95% confidence intervals. Black circles indicate associations between cytokine factors and RLUs/pg RT that were statistically significant (unadjusted P
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    88
    R&D Systems human ccl3 mip 1 alpha biotinylated antibody
    Preinfection genital inflammation was associated with acquisition of less infectious human immunodeficiency virus (HIV). A , Unsupervised hierarchical clustering was used to visualize the variation in cytokine concentrations in individual women and to cluster women according to the similarities of their cytokine expression profiles (using Qlucore Omics Explorer software, version 3.0). Women from whom less infectious viruses were isolated (value below median for relative light units per picogram of reverse-transcriptase [RLUs/pg RT]; n = 13; green blocks ) had up-regulated cytokine concentrations in preinfection cervicovaginal lavage (CVL) samples and tended to cluster together. Women from whom highly infectious viruses were isolated (RLUs/pg RT at or above the median) had lower cytokine concentrations and also clustered together (n = 14; yellow blocks ). Cytokine concentrations are indicated using a color scale, ranging from blue ( low ) to red ( high ). The dendrogram above the heat map illustrates degrees of relatedness between genital cytokine profiles evident among the various women. Patient identity numbers are indicated below the heat map. B , RLUs/pg RT and cytokine concentrations were either log 10 -transformed or converted to categorical variables <t>(MIP-1α,</t> MIP-1β, GM-CSF, and IL-7), and β coefficients were calculated using linear regression. Factor scores for each functional group of cytokines were generated using confirmatory factor analysis. Circles indicate β coefficients from regression analyses; error bars, 95% confidence intervals. Black circles indicate associations between cytokine factors and RLUs/pg RT that were statistically significant (unadjusted P
    Human Ccl3 Mip 1 Alpha Biotinylated Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    RayBiotech macrophage inflammatory protein mip 1 α
    PDX facilitated inflammation resolution in ALI. (a) Concentration of IL-1β; (b) concentration of TNF-α; (c) concentration of IL-6; (d) concentration of IL-10; (e) concentration of <t>MIP-2;</t> (f) concentration of <t>MIP-1α.</t> Data are mean ± SD. n = 6. * P
    Macrophage Inflammatory Protein Mip 1 α, supplied by RayBiotech, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    NEN Life Science 125 i labeled mip 1α
    Time course and specificity of <t>MIP-1α</t> receptor expression. ( A ) 125 I-labeled MIP-1α binding to uninfected ( UNINF ) or HCMV strain AD169-infected HFF cells was examined at the indicated time pi, in either the absence or presence of excess unlabeled MIP-1α ( h + MIP - 1 α) at a final concentration of 100 nM. cpm of bound radiolabeled MIP-1α are given above each bar. ( B ) Percentage of displaceable binding of 125 I-labeled MIP-1α using the indicated unlabeled chemokine (200 nM final concentration) in infected HFF cells at 72 h pi.
    125 I Labeled Mip 1α, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    R&D Systems anti human ccl3 mip1α
    Time course and specificity of <t>MIP-1α</t> receptor expression. ( A ) 125 I-labeled MIP-1α binding to uninfected ( UNINF ) or HCMV strain AD169-infected HFF cells was examined at the indicated time pi, in either the absence or presence of excess unlabeled MIP-1α ( h + MIP - 1 α) at a final concentration of 100 nM. cpm of bound radiolabeled MIP-1α are given above each bar. ( B ) Percentage of displaceable binding of 125 I-labeled MIP-1α using the indicated unlabeled chemokine (200 nM final concentration) in infected HFF cells at 72 h pi.
    Anti Human Ccl3 Mip1α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Koma Biotech mip 1α ccl3
    The effect of E. coli and V. vulnificus LPS on rat microglia chemokines macrophage inflammatory protein-2 <t>(MIP-2)/chemokine</t> (C-X-C motif) ligand 2 (CXCL2), <t>MIP-1α/chemokine</t> (C-C motif) ligand 3 <t>(CCL3),</t> CINC-2α/β/CXCL3 and monocyte chemotactic protein-1 (MCP-1)/CCL2 release. Neonatal rat microglia (2 × 10 5 cells/well) were treated with E. coli (0.1–100 ng/mL) or V. vulnificus (0.1–10 5 ng/mL) LPS for 17 h in vitro . MIP-2/CXCL2 (Panel A ), MIP-1α/CCL3 (Panel B ), CINC-2α/β/CXCL3 (Panel C ) and MCP-1/CCL2 (Panel D ) release was determined as described in Experimental Section . Data expressed as pg/mL is the mean ± SEM of two to four independent experiments ( n ), each experiment with duplicate determinations. * P
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    DCEO decreased mRNA levels of cytokines and chemokines in LPS-stimulated RAW264.7 cells. Notes: Cells were incubated with indicated concentrations of DCEO for 1 h and then stimulated with LPS for 6 h. The mRNA levels of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), MCP-1 ( D ), CCL-5 ( E ), and MIP-1α ( F ) were quantified by qRT-PCR. Data presented in bar charts are mean±SEM values from three independent experiments. ** P

    Journal: Drug Design, Development and Therapy

    Article Title: Dingchuan tang essential oil inhibits the production of inflammatory mediators via suppressing the IRAK/NF-κB, IRAK/AP-1, and TBK1/IRF3 pathways in lipopolysaccharide-stimulated RAW264.7 cells

    doi: 10.2147/DDDT.S160645

    Figure Lengend Snippet: DCEO decreased mRNA levels of cytokines and chemokines in LPS-stimulated RAW264.7 cells. Notes: Cells were incubated with indicated concentrations of DCEO for 1 h and then stimulated with LPS for 6 h. The mRNA levels of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), MCP-1 ( D ), CCL-5 ( E ), and MIP-1α ( F ) were quantified by qRT-PCR. Data presented in bar charts are mean±SEM values from three independent experiments. ** P

    Article Snippet: IL-1β, IL-6, TNF-α, monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α and chemokine (C-C motif) ligand 5 (CCL-5) enzyme-linked immunosorbent assay (ELISA) kits were obtained from eBioscience (San Diego, CA, USA).

    Techniques: Incubation, Quantitative RT-PCR

    DCEO reduced the production of cytokines and chemokines in LPS-stimulated RAW264.7 cells. Notes: Cells were incubated with indicated concentrations of DCEO for 1 h and then stimulated with LPS for 24 h. The production of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), MCP-1 ( D ), CCL-5 ( E ), and MIP-1α ( F ) was measured by ELISA. Data presented in bar charts are mean±SEM values from three independent experiments. ** P

    Journal: Drug Design, Development and Therapy

    Article Title: Dingchuan tang essential oil inhibits the production of inflammatory mediators via suppressing the IRAK/NF-κB, IRAK/AP-1, and TBK1/IRF3 pathways in lipopolysaccharide-stimulated RAW264.7 cells

    doi: 10.2147/DDDT.S160645

    Figure Lengend Snippet: DCEO reduced the production of cytokines and chemokines in LPS-stimulated RAW264.7 cells. Notes: Cells were incubated with indicated concentrations of DCEO for 1 h and then stimulated with LPS for 24 h. The production of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), MCP-1 ( D ), CCL-5 ( E ), and MIP-1α ( F ) was measured by ELISA. Data presented in bar charts are mean±SEM values from three independent experiments. ** P

    Article Snippet: IL-1β, IL-6, TNF-α, monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α and chemokine (C-C motif) ligand 5 (CCL-5) enzyme-linked immunosorbent assay (ELISA) kits were obtained from eBioscience (San Diego, CA, USA).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    Role of MIP-1α and CCR5 in Aβ 1–40 -induced synaptic disruption. A : Representative images of synaptophysin immunostaining in the CA1 subregion of the hippocampus evaluated 8 days after PBS, Aβ 1–40 , or Aβ 40–1 i.c.v. administration (400 pmol/mouse). Original magnification, ×40. B : Graphic representation of the average optical density (O.D.) of the immunostaining for synaptophysin evaluated in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus, demonstrating that MIP-1α −/− and CCR5 −/− mice were significantly more resistant than wild-type mice to Aβ 1–40 -induced synaptic disruption. The values represent the mean ± SEM ( N = 5 mice/group). ** P

    Journal: The American Journal of Pathology

    Article Title: Role of the Macrophage Inflammatory Protein-1?/CC Chemokine Receptor 5 Signaling Pathway in the Neuroinflammatory Response and Cognitive Deficits Induced by ?-Amyloid Peptide

    doi: 10.2353/ajpath.2009.081113

    Figure Lengend Snippet: Role of MIP-1α and CCR5 in Aβ 1–40 -induced synaptic disruption. A : Representative images of synaptophysin immunostaining in the CA1 subregion of the hippocampus evaluated 8 days after PBS, Aβ 1–40 , or Aβ 40–1 i.c.v. administration (400 pmol/mouse). Original magnification, ×40. B : Graphic representation of the average optical density (O.D.) of the immunostaining for synaptophysin evaluated in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus, demonstrating that MIP-1α −/− and CCR5 −/− mice were significantly more resistant than wild-type mice to Aβ 1–40 -induced synaptic disruption. The values represent the mean ± SEM ( N = 5 mice/group). ** P

    Article Snippet: Resident peritoneal macrophages were obtained from wild-type, CCR5−/− , or MIP-1α−/− mice and were stimulated with Aβ1–40 (30 μmol/L), Aβ40–1 (30 μmol/L), Escherichia coli lipopolysaccharide (LPS) (serotype 0111:B4, 100 ng/ml, Sigma-Aldrich), or complement component C5a (10 nmol/L, Sigma-Aldrich) for 24 hours.

    Techniques: Immunostaining, Mouse Assay

    Role of MIP-1α and CCR5 in intracellular pathways activated in response to Aβ 1–40 . Immunohistochemical analysis for p-CREB, p-p65 NF-κB, and p-c-Jun/AP-1 protein was performed 6 hours after Aβ 1–40 (400 pmol/mouse), Aβ 40–1 (400 pmol/mouse), or PBS i.c.v. injection. A : Graphic representation of the average immunostaining for p-CREB, demonstrating reduced activation in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus of MIP-1α −/− and CCR5 −/− mice. Similar results were observed when the number of positive cells for p-p65 NF-κB ( B ) and p-c-Jun AP-1 ( C ) per section was determined. The values represent the mean ± SEM ( N = 5 mice/group). ** P

    Journal: The American Journal of Pathology

    Article Title: Role of the Macrophage Inflammatory Protein-1?/CC Chemokine Receptor 5 Signaling Pathway in the Neuroinflammatory Response and Cognitive Deficits Induced by ?-Amyloid Peptide

    doi: 10.2353/ajpath.2009.081113

    Figure Lengend Snippet: Role of MIP-1α and CCR5 in intracellular pathways activated in response to Aβ 1–40 . Immunohistochemical analysis for p-CREB, p-p65 NF-κB, and p-c-Jun/AP-1 protein was performed 6 hours after Aβ 1–40 (400 pmol/mouse), Aβ 40–1 (400 pmol/mouse), or PBS i.c.v. injection. A : Graphic representation of the average immunostaining for p-CREB, demonstrating reduced activation in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus of MIP-1α −/− and CCR5 −/− mice. Similar results were observed when the number of positive cells for p-p65 NF-κB ( B ) and p-c-Jun AP-1 ( C ) per section was determined. The values represent the mean ± SEM ( N = 5 mice/group). ** P

    Article Snippet: Resident peritoneal macrophages were obtained from wild-type, CCR5−/− , or MIP-1α−/− mice and were stimulated with Aβ1–40 (30 μmol/L), Aβ40–1 (30 μmol/L), Escherichia coli lipopolysaccharide (LPS) (serotype 0111:B4, 100 ng/ml, Sigma-Aldrich), or complement component C5a (10 nmol/L, Sigma-Aldrich) for 24 hours.

    Techniques: Immunohistochemistry, Injection, Immunostaining, Activation Assay, Mouse Assay

    MIP-1α and CCR5 are required for cellular migration but not for activation induced by Aβ 1–40 in vitro . Peritoneal macrophages were isolated from wild-type (WT), MIP-1α −/− , or CCR5 −/− mice, cultured on 24-well plates, and incubated with 30 μmol/L Aβ 1–40 or Aβ 40–1 for 24 hours, and the supernatant was collected and assayed for chemotactic activity. Chemotaxis of wild-type, MIP-1α −/− , and CCR5 −/− macrophages in response to the supernatants was assayed using the Neuro Probe transwell assay. Chemotactic index represents the number of cells that migrated in response to supernatant from stimulated macrophages/number of cells that migrated in response to supernatant from unstimulated macrophages. A : MIP-1α −/− macrophages produce reduced chemotactic activity for wild-type, MIP-1α −/− , and CCR5 −/− cells when stimulated with Aβ 1–40 compared with wild-type and CCR5 −/− macrophages. Macrophages isolated from CCR5 −/− mice show a diminished chemotactic index in response to supernatant of Aβ 1–40 -stimulated wild-type, MIP-1α −/− , or CCR5 −/− cells. No significant chemotactic activity for macrophages was induced in response to the Aβ 40–1 . B : Macrophage chemotaxis toward C5a (10 nmol/L) was not affected by genetic deletion of MIP-1α or CCR5. IL-1β ( C ) or TNF-α ( D ) released into the supernatant of macrophages of wild-type, MIP-1α −/− , and CCR5 −/− mice stimulated for 24 hours with 30 μmol/L Aβ 1–40 or Aβ 40–1 , LPS (100 ng/ml), or C5a (10 nmol/L) was measured by enzyme-linked immunosorbent assay. E : Cell viability assessed after stimulation with Aβ 1–40 , Aβ 40–1 , LPS, or C5a. “Control” refers to unstimulated cells. Values represent the mean ± SEM ( N = 3/group). Chemotaxis assays were performed in triplicate, and ELISA experiments in duplicate. ** P

    Journal: The American Journal of Pathology

    Article Title: Role of the Macrophage Inflammatory Protein-1?/CC Chemokine Receptor 5 Signaling Pathway in the Neuroinflammatory Response and Cognitive Deficits Induced by ?-Amyloid Peptide

    doi: 10.2353/ajpath.2009.081113

    Figure Lengend Snippet: MIP-1α and CCR5 are required for cellular migration but not for activation induced by Aβ 1–40 in vitro . Peritoneal macrophages were isolated from wild-type (WT), MIP-1α −/− , or CCR5 −/− mice, cultured on 24-well plates, and incubated with 30 μmol/L Aβ 1–40 or Aβ 40–1 for 24 hours, and the supernatant was collected and assayed for chemotactic activity. Chemotaxis of wild-type, MIP-1α −/− , and CCR5 −/− macrophages in response to the supernatants was assayed using the Neuro Probe transwell assay. Chemotactic index represents the number of cells that migrated in response to supernatant from stimulated macrophages/number of cells that migrated in response to supernatant from unstimulated macrophages. A : MIP-1α −/− macrophages produce reduced chemotactic activity for wild-type, MIP-1α −/− , and CCR5 −/− cells when stimulated with Aβ 1–40 compared with wild-type and CCR5 −/− macrophages. Macrophages isolated from CCR5 −/− mice show a diminished chemotactic index in response to supernatant of Aβ 1–40 -stimulated wild-type, MIP-1α −/− , or CCR5 −/− cells. No significant chemotactic activity for macrophages was induced in response to the Aβ 40–1 . B : Macrophage chemotaxis toward C5a (10 nmol/L) was not affected by genetic deletion of MIP-1α or CCR5. IL-1β ( C ) or TNF-α ( D ) released into the supernatant of macrophages of wild-type, MIP-1α −/− , and CCR5 −/− mice stimulated for 24 hours with 30 μmol/L Aβ 1–40 or Aβ 40–1 , LPS (100 ng/ml), or C5a (10 nmol/L) was measured by enzyme-linked immunosorbent assay. E : Cell viability assessed after stimulation with Aβ 1–40 , Aβ 40–1 , LPS, or C5a. “Control” refers to unstimulated cells. Values represent the mean ± SEM ( N = 3/group). Chemotaxis assays were performed in triplicate, and ELISA experiments in duplicate. ** P

    Article Snippet: Resident peritoneal macrophages were obtained from wild-type, CCR5−/− , or MIP-1α−/− mice and were stimulated with Aβ1–40 (30 μmol/L), Aβ40–1 (30 μmol/L), Escherichia coli lipopolysaccharide (LPS) (serotype 0111:B4, 100 ng/ml, Sigma-Aldrich), or complement component C5a (10 nmol/L, Sigma-Aldrich) for 24 hours.

    Techniques: Migration, Activation Assay, In Vitro, Isolation, Mouse Assay, Cell Culture, Incubation, Activity Assay, Chemotaxis Assay, Transwell Assay, Enzyme-linked Immunosorbent Assay

    MIP-1α and CCR5 modulate Aβ 1–40 -induced iNOS and COX-2 up-regulation. A : Aβ 1–40 induced time-dependent iNOS and COX-2 protein expression in the hippocampus. Western blot analysis for iNOS, COX-2, and β-actin (loading control) was performed in the hippocampuses of wild-type C57BL/6 mice 1 and 8 days after Aβ 1–40 (400 pmol/mouse) i.c.v. injection. Some mice were left untreated (naive mice, N) or were treated with the reverse Aβ 40–1 peptide (400 pmol/mouse i.c.v.). B : MIP-1α and CCR5 genetic deletion resulted in a reduction of iNOS up-regulation induced by Aβ 1–40 , when evaluated 1 day after treatment. C : Graph showing quantification of iNOS protein normalized by β-actin protein (loading control). For COX-2 protein, immunohistochemical analysis was performed 1 day after Aβ 1–40 (400 pmol/mouse) or PBS i.c.v. injection. D : Representative images of COX-2 immunostaining, demonstrating diminished levels of this enzyme in the CA1 hippocampal subregion of MIP-1α −/− and CCR5 −/− mice. Original magnification, ×40 E : Graphic representation of the average immunostaining for COX-2 evaluated in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus. The values represent the mean ± SEM ( N = 6 to 7 mice/group). * P

    Journal: The American Journal of Pathology

    Article Title: Role of the Macrophage Inflammatory Protein-1?/CC Chemokine Receptor 5 Signaling Pathway in the Neuroinflammatory Response and Cognitive Deficits Induced by ?-Amyloid Peptide

    doi: 10.2353/ajpath.2009.081113

    Figure Lengend Snippet: MIP-1α and CCR5 modulate Aβ 1–40 -induced iNOS and COX-2 up-regulation. A : Aβ 1–40 induced time-dependent iNOS and COX-2 protein expression in the hippocampus. Western blot analysis for iNOS, COX-2, and β-actin (loading control) was performed in the hippocampuses of wild-type C57BL/6 mice 1 and 8 days after Aβ 1–40 (400 pmol/mouse) i.c.v. injection. Some mice were left untreated (naive mice, N) or were treated with the reverse Aβ 40–1 peptide (400 pmol/mouse i.c.v.). B : MIP-1α and CCR5 genetic deletion resulted in a reduction of iNOS up-regulation induced by Aβ 1–40 , when evaluated 1 day after treatment. C : Graph showing quantification of iNOS protein normalized by β-actin protein (loading control). For COX-2 protein, immunohistochemical analysis was performed 1 day after Aβ 1–40 (400 pmol/mouse) or PBS i.c.v. injection. D : Representative images of COX-2 immunostaining, demonstrating diminished levels of this enzyme in the CA1 hippocampal subregion of MIP-1α −/− and CCR5 −/− mice. Original magnification, ×40 E : Graphic representation of the average immunostaining for COX-2 evaluated in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus. The values represent the mean ± SEM ( N = 6 to 7 mice/group). * P

    Article Snippet: Resident peritoneal macrophages were obtained from wild-type, CCR5−/− , or MIP-1α−/− mice and were stimulated with Aβ1–40 (30 μmol/L), Aβ40–1 (30 μmol/L), Escherichia coli lipopolysaccharide (LPS) (serotype 0111:B4, 100 ng/ml, Sigma-Aldrich), or complement component C5a (10 nmol/L, Sigma-Aldrich) for 24 hours.

    Techniques: Expressing, Western Blot, Mouse Assay, Injection, Immunohistochemistry, Immunostaining

    Effect of Aβ 1–40 on the expression of MIP-1α and CCR5 and in glial cell activation in mouse hippocampus. Wild-type C57BL/6 mice were left untreated (naive mice, N) or were treated i.c.v. with Aβ 1–40 or Aβ 40–1 (400 pmol/mouse), and brains were harvested at the time points indicated. Total RNA was isolated from hippocampuses for evaluation of MIP-1α and CCR5 mRNA expression, and β-actin mRNA was assessed as an internal control for the amount of RNA in each sample. Representative RT-PCR analysis showing MIP-1α ( A ) and CCR5 mRNA ( B ) expression. Densitometric analysis is expressed as the MIP-1α/β-actin ( C ) and the CCR5/β-actin ratio ( D ). E : Representative images of GFAP and CD68 immunostaining in the CA1 subregion of the hippocampus. Original magnification, ×100. Graphic representation of the number of GFAP- ( F ) and CD68-positive cells ( G ) determined in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus. The values represent the mean ± SEM ( N = 3 to 5 mice/group). * P

    Journal: The American Journal of Pathology

    Article Title: Role of the Macrophage Inflammatory Protein-1?/CC Chemokine Receptor 5 Signaling Pathway in the Neuroinflammatory Response and Cognitive Deficits Induced by ?-Amyloid Peptide

    doi: 10.2353/ajpath.2009.081113

    Figure Lengend Snippet: Effect of Aβ 1–40 on the expression of MIP-1α and CCR5 and in glial cell activation in mouse hippocampus. Wild-type C57BL/6 mice were left untreated (naive mice, N) or were treated i.c.v. with Aβ 1–40 or Aβ 40–1 (400 pmol/mouse), and brains were harvested at the time points indicated. Total RNA was isolated from hippocampuses for evaluation of MIP-1α and CCR5 mRNA expression, and β-actin mRNA was assessed as an internal control for the amount of RNA in each sample. Representative RT-PCR analysis showing MIP-1α ( A ) and CCR5 mRNA ( B ) expression. Densitometric analysis is expressed as the MIP-1α/β-actin ( C ) and the CCR5/β-actin ratio ( D ). E : Representative images of GFAP and CD68 immunostaining in the CA1 subregion of the hippocampus. Original magnification, ×100. Graphic representation of the number of GFAP- ( F ) and CD68-positive cells ( G ) determined in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus. The values represent the mean ± SEM ( N = 3 to 5 mice/group). * P

    Article Snippet: Resident peritoneal macrophages were obtained from wild-type, CCR5−/− , or MIP-1α−/− mice and were stimulated with Aβ1–40 (30 μmol/L), Aβ40–1 (30 μmol/L), Escherichia coli lipopolysaccharide (LPS) (serotype 0111:B4, 100 ng/ml, Sigma-Aldrich), or complement component C5a (10 nmol/L, Sigma-Aldrich) for 24 hours.

    Techniques: Expressing, Activation Assay, Mouse Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Immunostaining

    Involvement of MIP-1α and CCR5 in Aβ 1–40 -induced glial cell activation in mouse hippocampus. Immunohistochemical analysis for GFAP and CD68 was performed 6 hours or 8 days after Aβ 1–40 (400 pmol/mouse) or PBS i.c.v. injection, respectively. Mice lacking MIP-1α or CCR5 showed a reduced number of GFAP- ( A and C ) and CD68-positive cells ( B and D ) in the hippocampus. Representative images of GFAP ( A ) and CD68 immunostaining ( B ) in the CA1 subregion of the hippocampus. Original magnification, ×100. Graphic representation of the number of GFAP- ( C ) and CD68-positive cells ( D ) determined in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus. The values represent the mean ± SEM ( N = 5 mice/group). ** P

    Journal: The American Journal of Pathology

    Article Title: Role of the Macrophage Inflammatory Protein-1?/CC Chemokine Receptor 5 Signaling Pathway in the Neuroinflammatory Response and Cognitive Deficits Induced by ?-Amyloid Peptide

    doi: 10.2353/ajpath.2009.081113

    Figure Lengend Snippet: Involvement of MIP-1α and CCR5 in Aβ 1–40 -induced glial cell activation in mouse hippocampus. Immunohistochemical analysis for GFAP and CD68 was performed 6 hours or 8 days after Aβ 1–40 (400 pmol/mouse) or PBS i.c.v. injection, respectively. Mice lacking MIP-1α or CCR5 showed a reduced number of GFAP- ( A and C ) and CD68-positive cells ( B and D ) in the hippocampus. Representative images of GFAP ( A ) and CD68 immunostaining ( B ) in the CA1 subregion of the hippocampus. Original magnification, ×100. Graphic representation of the number of GFAP- ( C ) and CD68-positive cells ( D ) determined in the CA1, CA2, CA3, and dentate gyrus subregions of the hippocampus. The values represent the mean ± SEM ( N = 5 mice/group). ** P

    Article Snippet: Resident peritoneal macrophages were obtained from wild-type, CCR5−/− , or MIP-1α−/− mice and were stimulated with Aβ1–40 (30 μmol/L), Aβ40–1 (30 μmol/L), Escherichia coli lipopolysaccharide (LPS) (serotype 0111:B4, 100 ng/ml, Sigma-Aldrich), or complement component C5a (10 nmol/L, Sigma-Aldrich) for 24 hours.

    Techniques: Activation Assay, Immunohistochemistry, Injection, Mouse Assay, Immunostaining

    MIP-1α and MIP-1β expression in LPS-primed neonatal and adult monocytes following co-stimulation with Ureaplasma isolates. Relative quantification of chemokine mRNA (A, B) and concentrations of secreted protein (C, D) are presented as mean ± SD ( ○ p

    Journal: PLoS ONE

    Article Title: Ureaplasma isolates stimulate pro-inflammatory CC chemokines and matrix metalloproteinase-9 in neonatal and adult monocytes

    doi: 10.1371/journal.pone.0194514

    Figure Lengend Snippet: MIP-1α and MIP-1β expression in LPS-primed neonatal and adult monocytes following co-stimulation with Ureaplasma isolates. Relative quantification of chemokine mRNA (A, B) and concentrations of secreted protein (C, D) are presented as mean ± SD ( ○ p

    Article Snippet: For quantitative detection of MIP-1α, MIP-1β, MCP-1, MCP-3, and MMP-9 mRNA, cDNA was diluted 1: 10 in deionized, nuclease-free water and analyzed in duplicates of 25 μl using 12.5 μl iTaq™ Universal SYBR Green Supermix (Bio-Rad Laboratories, USA).

    Techniques: Expressing

    Neonatal and adult human monocytes display elevated levels of MIP-1α and MIP-1β mRNA and protein upon stimulation with Uu and Up. Chemokine expression was assessed at the level of mRNA (A, B) and protein secretion (C, D). The values represent the means ± SD (* p

    Journal: PLoS ONE

    Article Title: Ureaplasma isolates stimulate pro-inflammatory CC chemokines and matrix metalloproteinase-9 in neonatal and adult monocytes

    doi: 10.1371/journal.pone.0194514

    Figure Lengend Snippet: Neonatal and adult human monocytes display elevated levels of MIP-1α and MIP-1β mRNA and protein upon stimulation with Uu and Up. Chemokine expression was assessed at the level of mRNA (A, B) and protein secretion (C, D). The values represent the means ± SD (* p

    Article Snippet: For quantitative detection of MIP-1α, MIP-1β, MCP-1, MCP-3, and MMP-9 mRNA, cDNA was diluted 1: 10 in deionized, nuclease-free water and analyzed in duplicates of 25 μl using 12.5 μl iTaq™ Universal SYBR Green Supermix (Bio-Rad Laboratories, USA).

    Techniques: Expressing

    Functional characteristics of LentiVIP-DC. ( a ) Untransduced-, LentiGFP-, and LentiVIP-DC were incubated in medium containing 1 mg/ml of dextran-PE (40 kd) for 2 hours at 4 °C (control; open histograms) or 37 °C (filled histograms), extensively washed and analyzed by flow cytometry. One representative experiment of two performed in duplicate is shown. ( b ) Untransduced-, LentiGFP-, and LentiVIP-DC were cultured with LPS (1 µg/ml) for 24 hours. Culture supernatants were assayed for IL-12p70, IL-1β, TNF-α, IL-6, MIP-1α, and IL-10. Results are the mean ± SD of three experiments performed in duplicate. Degree of significance P

    Journal: Molecular Therapy

    Article Title: Dendritic Cells Transduced With Lentiviral Vectors Expressing VIP Differentiate Into VIP-secreting Tolerogenic-like DCs

    doi: 10.1038/mt.2009.293

    Figure Lengend Snippet: Functional characteristics of LentiVIP-DC. ( a ) Untransduced-, LentiGFP-, and LentiVIP-DC were incubated in medium containing 1 mg/ml of dextran-PE (40 kd) for 2 hours at 4 °C (control; open histograms) or 37 °C (filled histograms), extensively washed and analyzed by flow cytometry. One representative experiment of two performed in duplicate is shown. ( b ) Untransduced-, LentiGFP-, and LentiVIP-DC were cultured with LPS (1 µg/ml) for 24 hours. Culture supernatants were assayed for IL-12p70, IL-1β, TNF-α, IL-6, MIP-1α, and IL-10. Results are the mean ± SD of three experiments performed in duplicate. Degree of significance P

    Article Snippet: Capture and biotinylated anti-mouse IL-12p70, TNF-α, IL-6, IL-10, IL-1β, MIP-1α were purchased from BD Biosciences Pharmingen (San Diego, CA).

    Techniques: Functional Assay, Incubation, Flow Cytometry, Cytometry, Cell Culture

    MIP-1α ( a ) and MCP-1 ( b ) production by mouse PMN after 2 and 24 h stimulation with bacterial filtrates obtained from planktonic (PL) or biofilm (B) cultures of S. aureus strains (8325-4, Wood 46, Sa-α11, Sa-α21. c Unstimulated control cells. All data are the means ± SD. *A significant differences ( p

    Journal: Archivum Immunologiae et Therapiae Experimentalis

    Article Title: The Immunomodulatory Activity of Staphylococcus aureus Products Derived from Biofilm and Planktonic Cultures

    doi: 10.1007/s00005-013-0240-3

    Figure Lengend Snippet: MIP-1α ( a ) and MCP-1 ( b ) production by mouse PMN after 2 and 24 h stimulation with bacterial filtrates obtained from planktonic (PL) or biofilm (B) cultures of S. aureus strains (8325-4, Wood 46, Sa-α11, Sa-α21. c Unstimulated control cells. All data are the means ± SD. *A significant differences ( p

    Article Snippet: The ELISA Development Kits specific for murine TNF-α, JE/MCP-1, MIP-1α (PeproTech, France) and murine IL-6 or IL-10 Eli-pair (Diaclone, France) were used according to the manufacturer’s instruction.

    Techniques:

    Ex vivo cytokines production upon TLR7 or TLR8 agonist stimulation during whole blood culture. Fresh whole blood specimens from male volunteers were stimulated with TLR7 agonist (3M-001) (A–E) or TLR8 agonist (3M-002) (F–J) for 12 hr. The concentrations of IFN-α, IL-1β, IL-6, TNF-α, and MIP-1α concentrations in the supernatant were measured by high sensitive IFN-α ELISA and Luminex100 system. Each dot represents an individual, (•) represent individuals with TLR7 IVS2-151A/TLR8 -129G genotype, (▪) represent individuals with TLR7 IVS2-151G/TLR8 -129G genotype and (▴) represent those with TLR7 IVS2-151A/TLR8 -129C genotype. The horizontal bars represent mean value.

    Journal: PLoS ONE

    Article Title: TLR7 and TLR8 Gene Variations and Susceptibility to Hepatitis C Virus Infection

    doi: 10.1371/journal.pone.0026235

    Figure Lengend Snippet: Ex vivo cytokines production upon TLR7 or TLR8 agonist stimulation during whole blood culture. Fresh whole blood specimens from male volunteers were stimulated with TLR7 agonist (3M-001) (A–E) or TLR8 agonist (3M-002) (F–J) for 12 hr. The concentrations of IFN-α, IL-1β, IL-6, TNF-α, and MIP-1α concentrations in the supernatant were measured by high sensitive IFN-α ELISA and Luminex100 system. Each dot represents an individual, (•) represent individuals with TLR7 IVS2-151A/TLR8 -129G genotype, (▪) represent individuals with TLR7 IVS2-151G/TLR8 -129G genotype and (▴) represent those with TLR7 IVS2-151A/TLR8 -129C genotype. The horizontal bars represent mean value.

    Article Snippet: The combinations of different types of beads allows for the simultaneous measurements of IFN-α, IL-1β, IL-6, TNF-α, and MIP-1α (Biosource International, Camarillo, CA).

    Techniques: Ex Vivo, Enzyme-linked Immunosorbent Assay

    Chemokine levels in lung tissue. N = 7/group. LPS injection increased levels of MIP-1α (A, p

    Journal: PLoS ONE

    Article Title: MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia

    doi: 10.1371/journal.pone.0039940

    Figure Lengend Snippet: Chemokine levels in lung tissue. N = 7/group. LPS injection increased levels of MIP-1α (A, p

    Article Snippet: Then, proteins were transferred to PVDF membranes, blocked in non-fat milk or bovine albumin as needed, and incubated with antibodies against S100A6 (R & D Systems), S100A8 (R & D Systems), S100A9 (R & D Systems), MIP-2 (AbD serotec), LIX (Peprotech), MIP-1α (Abcam), IL-10 (Abcam), p65 (phosphorylated and total, Abcam), p52 (Cell signaling) and actin (Santa Cruz Biotechnology #SC1616).

    Techniques: Injection

    Characteristic of microglial cells treated with DHE using a flow cytometer. (A) Dot plot of microglia to gate CD11b-positive and CD206-positive cells treated either with or without DHE. (B) Ratio (%) of CD11b-positive and CD206-positive cells to CD11b-single positive cells. (C-F) Median fluorescent intensity of I-A/I-E, CD86, CD80 and CD68 in CD11b-positive cells. (G-J) Median fluorescent intensity of I-A/I-E, CD86, CD80 and CD68 in CD11b-positive and CD206-positive cells. K-N. Ratio (%) of TNF-α, IL-1β, MIP-1αin IL-12p40/p70-positive to CD11b-positive cells, respectively. O-R. Ratio (%) of TNF-α, IL-1β, MIP-1α and IL-12p40/p70-positive in CD11b-positive and CD206-positive cells, respectively.

    Journal: PLoS ONE

    Article Title: Identification of a Novel Dehydroergosterol Enhancing Microglial Anti-Inflammatory Activity in a Dairy Product Fermented with Penicillium candidum

    doi: 10.1371/journal.pone.0116598

    Figure Lengend Snippet: Characteristic of microglial cells treated with DHE using a flow cytometer. (A) Dot plot of microglia to gate CD11b-positive and CD206-positive cells treated either with or without DHE. (B) Ratio (%) of CD11b-positive and CD206-positive cells to CD11b-single positive cells. (C-F) Median fluorescent intensity of I-A/I-E, CD86, CD80 and CD68 in CD11b-positive cells. (G-J) Median fluorescent intensity of I-A/I-E, CD86, CD80 and CD68 in CD11b-positive and CD206-positive cells. K-N. Ratio (%) of TNF-α, IL-1β, MIP-1αin IL-12p40/p70-positive to CD11b-positive cells, respectively. O-R. Ratio (%) of TNF-α, IL-1β, MIP-1α and IL-12p40/p70-positive in CD11b-positive and CD206-positive cells, respectively.

    Article Snippet: To measure intracellular cytokine production, microglial cells were treated with the leukocyte activation cocktail with BD GolgiPlug (BD Biosciences) for 12 hours and with BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences), and then stained with the following antibodies; anti-MIP-1α-PE (eBioscience), anti-TNF-α-FITC (eBioscience), anti-interleukin-1β (IL-1β)-FITC (eBioscience), anti-IL-12p40/p70-APC (BD Pharmingen), anti-CD11b-APC-Cy7, and anti-CD206-PerCP-Cy5.5.

    Techniques: Flow Cytometry, Cytometry

    CyaA-induced cytokine and chemokine production is altered in TLR4-defective mice. Bone marrow derived DC from C3H/HeN or C3H/HeJ mice (10 6 /ml) were cultured with the indicated concentrations of LPS (0 to 10 ng/ml), CyaA (1 μg/ml), in the presence or absence of polymyxin B (PB) (10 μg/ml). Supernatants were tested by immunoassay for IL-10 and MIP-1α (4 h) and IL-12p70 and TNF-α (24 h). Results are means (error bars, SD) of triplicate assays and are representative of three experiments. Symbols: **, P

    Journal: Infection and Immunity

    Article Title: Adenylate Cyclase Toxin from Bordetella pertussis Synergizes with Lipopolysaccharide To Promote Innate Interleukin-10 Production and Enhances the Induction of Th2 and Regulatory T Cells

    doi: 10.1128/IAI.72.3.1568-1579.2004

    Figure Lengend Snippet: CyaA-induced cytokine and chemokine production is altered in TLR4-defective mice. Bone marrow derived DC from C3H/HeN or C3H/HeJ mice (10 6 /ml) were cultured with the indicated concentrations of LPS (0 to 10 ng/ml), CyaA (1 μg/ml), in the presence or absence of polymyxin B (PB) (10 μg/ml). Supernatants were tested by immunoassay for IL-10 and MIP-1α (4 h) and IL-12p70 and TNF-α (24 h). Results are means (error bars, SD) of triplicate assays and are representative of three experiments. Symbols: **, P

    Article Snippet: Concentrations of IL-6 and macrophage inflammatory protein (MIP)-1α were determined by immunoassay using pairs of antibodies and recombinant cytokines as standards (BD Pharmingen).

    Techniques: Mouse Assay, Derivative Assay, Cell Culture

    Relative variation in β-chemokine production by HIV-1-infected CD4 + T cells versus the uninfected controls. The differences among the levels of MIP-1α, MIP-1β, and RANTES produced by CD4 + T cells infected with several viral isolates (NSI, SV [○], NB [□], and LSP [▵]; SI, KP [▴], EM [■], and SF2 [•]) and their concurrent control cultures (uninfected cells from the same donors) are shown. Cells from seven different donors were used. Purified CD4 + T cells were stimulated for 3 days with 3 μg of PHA per ml, washed, and subsequently infected with various NSI and SI virus strains and cultured as described in Materials and Methods. Each symbol represents results with the virus in a separate experiment. The concentration of chemokines in the cell culture fluids was measured by ELISA in duplicate (Quantikine; R D Systems). The levels of chemokines measured in culture fluids from day 13 are shown (one measurement from day 11 of cells plated at 10 6 on day 8 is included) in reference to the level in the control cultures. Control level ranges (picograms per milliliter): MIP-1α, 58 to 4,261; MIP-1β, 46 to 1,117; RANTES, undetectable to 38. All results are adjusted per 10 6 viable cells. Cell numbers (10 6 ) on day 13: control ( n = 6), 1.2 ± 0.21; NSI ( n = 8), 0.93 ± 0.24; SI ( n = 9), 0.67 ± 0.17. Compared to control cultures, NSI viruses increased MIP-1α and MIP-1β production significantly ( P

    Journal: Journal of Virology

    Article Title: Differential Effects of Human Immunodeficiency Virus Isolates on ?-Chemokine and Gamma Interferon Production and on Cell Proliferation

    doi:

    Figure Lengend Snippet: Relative variation in β-chemokine production by HIV-1-infected CD4 + T cells versus the uninfected controls. The differences among the levels of MIP-1α, MIP-1β, and RANTES produced by CD4 + T cells infected with several viral isolates (NSI, SV [○], NB [□], and LSP [▵]; SI, KP [▴], EM [■], and SF2 [•]) and their concurrent control cultures (uninfected cells from the same donors) are shown. Cells from seven different donors were used. Purified CD4 + T cells were stimulated for 3 days with 3 μg of PHA per ml, washed, and subsequently infected with various NSI and SI virus strains and cultured as described in Materials and Methods. Each symbol represents results with the virus in a separate experiment. The concentration of chemokines in the cell culture fluids was measured by ELISA in duplicate (Quantikine; R D Systems). The levels of chemokines measured in culture fluids from day 13 are shown (one measurement from day 11 of cells plated at 10 6 on day 8 is included) in reference to the level in the control cultures. Control level ranges (picograms per milliliter): MIP-1α, 58 to 4,261; MIP-1β, 46 to 1,117; RANTES, undetectable to 38. All results are adjusted per 10 6 viable cells. Cell numbers (10 6 ) on day 13: control ( n = 6), 1.2 ± 0.21; NSI ( n = 8), 0.93 ± 0.24; SI ( n = 9), 0.67 ± 0.17. Compared to control cultures, NSI viruses increased MIP-1α and MIP-1β production significantly ( P

    Article Snippet: Monoclonal antibody for MIP-1α (mouse anti-human MIP-1α–phycoerythrin) was purchased from Pharmingen.

    Techniques: Infection, Produced, Purification, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Kinetics of virus replication, number of infected cells, and production of MIP-1α in CD4 + T cells infected with NSI and SI virus isolates. Purified CD4 + T cells from the same donor were stimulated for 3 days with 3 μg of PHA per ml, washed, and subsequently infected with 10 TCID 50 of the NSI virus isolate SV (○) or the SI virus isolate KP (▴) per 10 6 cells. After infection, the cultures were passaged every 2 to 3 days by exchanging the entire amount of culture fluid with fresh culture medium. On day 7 and thereafter, cells were replated at 10 6 ) (A). The percentage of productively infected CD4 + T cells was determined by flow cytometry measuring the intracellular presence of the viral p24 antigen (B). As a positive control, the chronically infected E line was used, showing > 97% p24 + cells. In the uninfected control, the p24 core antigen was undetectable (

    Journal: Journal of Virology

    Article Title: Differential Effects of Human Immunodeficiency Virus Isolates on ?-Chemokine and Gamma Interferon Production and on Cell Proliferation

    doi:

    Figure Lengend Snippet: Kinetics of virus replication, number of infected cells, and production of MIP-1α in CD4 + T cells infected with NSI and SI virus isolates. Purified CD4 + T cells from the same donor were stimulated for 3 days with 3 μg of PHA per ml, washed, and subsequently infected with 10 TCID 50 of the NSI virus isolate SV (○) or the SI virus isolate KP (▴) per 10 6 cells. After infection, the cultures were passaged every 2 to 3 days by exchanging the entire amount of culture fluid with fresh culture medium. On day 7 and thereafter, cells were replated at 10 6 ) (A). The percentage of productively infected CD4 + T cells was determined by flow cytometry measuring the intracellular presence of the viral p24 antigen (B). As a positive control, the chronically infected E line was used, showing > 97% p24 + cells. In the uninfected control, the p24 core antigen was undetectable (

    Article Snippet: Monoclonal antibody for MIP-1α (mouse anti-human MIP-1α–phycoerythrin) was purchased from Pharmingen.

    Techniques: Infection, Purification, Flow Cytometry, Cytometry, Positive Control

    Effect of TGF-β1 on IL-8 and MIP-1α expression. (a) IL-8 mRNA expression was analysed in RA, OA and non-arthritic FLS treated with TGF-β1 for 2 or 4 h. RT-PCR was performed as described in Materials and methods. (b) IL-8 protein expression was analysed by immunoblotting using anti-IL-8 antibody. RA5 FLS were stimulated with TGF-β and/or TNFα for 12h in the absence (lanes 1–4) or presence (lanes 5–8) of BFA (0·5 μg/ml). (c) MIP-1α mRNA expression was analysed by RT-PCR as in (a).

    Journal: Clinical and Experimental Immunology

    Article Title: Increased expression of pro-inflammatory cytokines and metalloproteinase-1 by TGF-?1 in synovial fibroblasts from rheumatoid arthritis and normal individuals

    doi: 10.1046/j.1365-2249.2002.01785.x

    Figure Lengend Snippet: Effect of TGF-β1 on IL-8 and MIP-1α expression. (a) IL-8 mRNA expression was analysed in RA, OA and non-arthritic FLS treated with TGF-β1 for 2 or 4 h. RT-PCR was performed as described in Materials and methods. (b) IL-8 protein expression was analysed by immunoblotting using anti-IL-8 antibody. RA5 FLS were stimulated with TGF-β and/or TNFα for 12h in the absence (lanes 1–4) or presence (lanes 5–8) of BFA (0·5 μg/ml). (c) MIP-1α mRNA expression was analysed by RT-PCR as in (a).

    Article Snippet: Antibody for IL-1β was purchased from R & D systems (Minneapolis, MN, USA) and antibodies for IL-8 and MIP-1α from Santa Cruz Biotechnology (Santa Cruz, CA, USA), while antibody for MMP-1 was from Oncogene (Cambridge, MA, USA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Preinfection genital inflammation was associated with acquisition of less infectious human immunodeficiency virus (HIV). A , Unsupervised hierarchical clustering was used to visualize the variation in cytokine concentrations in individual women and to cluster women according to the similarities of their cytokine expression profiles (using Qlucore Omics Explorer software, version 3.0). Women from whom less infectious viruses were isolated (value below median for relative light units per picogram of reverse-transcriptase [RLUs/pg RT]; n = 13; green blocks ) had up-regulated cytokine concentrations in preinfection cervicovaginal lavage (CVL) samples and tended to cluster together. Women from whom highly infectious viruses were isolated (RLUs/pg RT at or above the median) had lower cytokine concentrations and also clustered together (n = 14; yellow blocks ). Cytokine concentrations are indicated using a color scale, ranging from blue ( low ) to red ( high ). The dendrogram above the heat map illustrates degrees of relatedness between genital cytokine profiles evident among the various women. Patient identity numbers are indicated below the heat map. B , RLUs/pg RT and cytokine concentrations were either log 10 -transformed or converted to categorical variables (MIP-1α, MIP-1β, GM-CSF, and IL-7), and β coefficients were calculated using linear regression. Factor scores for each functional group of cytokines were generated using confirmatory factor analysis. Circles indicate β coefficients from regression analyses; error bars, 95% confidence intervals. Black circles indicate associations between cytokine factors and RLUs/pg RT that were statistically significant (unadjusted P

    Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

    Article Title: Cervicovaginal Inflammation Facilitates Acquisition of Less Infectious HIV Variants

    doi: 10.1093/cid/ciw663

    Figure Lengend Snippet: Preinfection genital inflammation was associated with acquisition of less infectious human immunodeficiency virus (HIV). A , Unsupervised hierarchical clustering was used to visualize the variation in cytokine concentrations in individual women and to cluster women according to the similarities of their cytokine expression profiles (using Qlucore Omics Explorer software, version 3.0). Women from whom less infectious viruses were isolated (value below median for relative light units per picogram of reverse-transcriptase [RLUs/pg RT]; n = 13; green blocks ) had up-regulated cytokine concentrations in preinfection cervicovaginal lavage (CVL) samples and tended to cluster together. Women from whom highly infectious viruses were isolated (RLUs/pg RT at or above the median) had lower cytokine concentrations and also clustered together (n = 14; yellow blocks ). Cytokine concentrations are indicated using a color scale, ranging from blue ( low ) to red ( high ). The dendrogram above the heat map illustrates degrees of relatedness between genital cytokine profiles evident among the various women. Patient identity numbers are indicated below the heat map. B , RLUs/pg RT and cytokine concentrations were either log 10 -transformed or converted to categorical variables (MIP-1α, MIP-1β, GM-CSF, and IL-7), and β coefficients were calculated using linear regression. Factor scores for each functional group of cytokines were generated using confirmatory factor analysis. Circles indicate β coefficients from regression analyses; error bars, 95% confidence intervals. Black circles indicate associations between cytokine factors and RLUs/pg RT that were statistically significant (unadjusted P

    Article Snippet: Concentrations of interleukin 1α, 1β, 6, 7, 8, and 10 (IL-1α, IL-1β, IL-6, IL-7, IL-8, and IL-10), granulocyte-macrophage colony-stimulating factor, interferon γ–inducible protein 10, monocyte chemoattractant protein 1, macrophage inflammatory protein (MIP) 1α, MIP-1β, and tumor necrosis factor (TNF) α were measured in CVL samples, as described elsewhere, using Milliplex Human Cytokine kits (Millipore) [ ].

    Techniques: Expressing, Software, Isolation, Transformation Assay, Functional Assay, Generated

    PDX facilitated inflammation resolution in ALI. (a) Concentration of IL-1β; (b) concentration of TNF-α; (c) concentration of IL-6; (d) concentration of IL-10; (e) concentration of MIP-2; (f) concentration of MIP-1α. Data are mean ± SD. n = 6. * P

    Journal: Chinese Medical Journal

    Article Title: Protectin DX Exhibits Protective Effects in Mouse Model of Lipopolysaccharide-Induced Acute Lung Injury

    doi: 10.4103/0366-6999.227618

    Figure Lengend Snippet: PDX facilitated inflammation resolution in ALI. (a) Concentration of IL-1β; (b) concentration of TNF-α; (c) concentration of IL-6; (d) concentration of IL-10; (e) concentration of MIP-2; (f) concentration of MIP-1α. Data are mean ± SD. n = 6. * P

    Article Snippet: Inflammatory cytokines' analysis in bronchoalveolar lavage fluid The concentrations of interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-alpha (TNF-α), macrophage inflammatory protein (MIP)-1α, and MIP-2 in BALF were determined using an enzyme-linked immunosorbent assay (ELISA) kit (RayBiotech Inc., Norcross, GA, USA) to assess pulmonary inflammation.

    Techniques: Concentration Assay

    Time course and specificity of MIP-1α receptor expression. ( A ) 125 I-labeled MIP-1α binding to uninfected ( UNINF ) or HCMV strain AD169-infected HFF cells was examined at the indicated time pi, in either the absence or presence of excess unlabeled MIP-1α ( h + MIP - 1 α) at a final concentration of 100 nM. cpm of bound radiolabeled MIP-1α are given above each bar. ( B ) Percentage of displaceable binding of 125 I-labeled MIP-1α using the indicated unlabeled chemokine (200 nM final concentration) in infected HFF cells at 72 h pi.

    Journal: The Journal of Experimental Medicine

    Article Title: Chemokine Sequestration by Viral Chemoreceptors as a Novel Viral Escape Strategy: Withdrawal of Chemokines from the Environment of Cytomegalovirus-infected Cells

    doi:

    Figure Lengend Snippet: Time course and specificity of MIP-1α receptor expression. ( A ) 125 I-labeled MIP-1α binding to uninfected ( UNINF ) or HCMV strain AD169-infected HFF cells was examined at the indicated time pi, in either the absence or presence of excess unlabeled MIP-1α ( h + MIP - 1 α) at a final concentration of 100 nM. cpm of bound radiolabeled MIP-1α are given above each bar. ( B ) Percentage of displaceable binding of 125 I-labeled MIP-1α using the indicated unlabeled chemokine (200 nM final concentration) in infected HFF cells at 72 h pi.

    Article Snippet: For infected cell binding studies, 125 I-labeled MIP-1α (2,200Ci/ mmol) was purchased from NEN™ Life Science Products (Boston, MA); unlabeled chemokines MCP-1, MCP-2, MCP-3, RANTES, MIP-1α, and MIP-1β were purchased from Pepro Tech (Rocky Hills, NJ).

    Techniques: Expressing, Labeling, Binding Assay, Infection, Concentration Assay

    MIP-1α binding by cells infected with HCMV mutants. Uninfected or HCMV-infected HFF cells (multiplicity of infection 2.5) were analyzed for their ability to bind 125 I-labeled MIP-1α at 72 h pi. RV92-2 and RV92-15 are independent isolates containing the identical US28 deletion. In some experiments, excess unlabeled MIP-1α (200 nM final concentration) was used ( AD169 + MIP - 1 α). cpm of bound radiolabeled MIP-1α are given above each bar.

    Journal: The Journal of Experimental Medicine

    Article Title: Chemokine Sequestration by Viral Chemoreceptors as a Novel Viral Escape Strategy: Withdrawal of Chemokines from the Environment of Cytomegalovirus-infected Cells

    doi:

    Figure Lengend Snippet: MIP-1α binding by cells infected with HCMV mutants. Uninfected or HCMV-infected HFF cells (multiplicity of infection 2.5) were analyzed for their ability to bind 125 I-labeled MIP-1α at 72 h pi. RV92-2 and RV92-15 are independent isolates containing the identical US28 deletion. In some experiments, excess unlabeled MIP-1α (200 nM final concentration) was used ( AD169 + MIP - 1 α). cpm of bound radiolabeled MIP-1α are given above each bar.

    Article Snippet: For infected cell binding studies, 125 I-labeled MIP-1α (2,200Ci/ mmol) was purchased from NEN™ Life Science Products (Boston, MA); unlabeled chemokines MCP-1, MCP-2, MCP-3, RANTES, MIP-1α, and MIP-1β were purchased from Pepro Tech (Rocky Hills, NJ).

    Techniques: Binding Assay, Infection, Labeling, Concentration Assay

    The effect of E. coli and V. vulnificus LPS on rat microglia chemokines macrophage inflammatory protein-2 (MIP-2)/chemokine (C-X-C motif) ligand 2 (CXCL2), MIP-1α/chemokine (C-C motif) ligand 3 (CCL3), CINC-2α/β/CXCL3 and monocyte chemotactic protein-1 (MCP-1)/CCL2 release. Neonatal rat microglia (2 × 10 5 cells/well) were treated with E. coli (0.1–100 ng/mL) or V. vulnificus (0.1–10 5 ng/mL) LPS for 17 h in vitro . MIP-2/CXCL2 (Panel A ), MIP-1α/CCL3 (Panel B ), CINC-2α/β/CXCL3 (Panel C ) and MCP-1/CCL2 (Panel D ) release was determined as described in Experimental Section . Data expressed as pg/mL is the mean ± SEM of two to four independent experiments ( n ), each experiment with duplicate determinations. * P

    Journal: Marine Drugs

    Article Title: Vibrio vulnificus MO6-24/O Lipopolysaccharide Stimulates Superoxide Anion, Thromboxane B2, Matrix Metalloproteinase-9, Cytokine and Chemokine Release by Rat Brain Microglia in Vitro

    doi: 10.3390/md12041732

    Figure Lengend Snippet: The effect of E. coli and V. vulnificus LPS on rat microglia chemokines macrophage inflammatory protein-2 (MIP-2)/chemokine (C-X-C motif) ligand 2 (CXCL2), MIP-1α/chemokine (C-C motif) ligand 3 (CCL3), CINC-2α/β/CXCL3 and monocyte chemotactic protein-1 (MCP-1)/CCL2 release. Neonatal rat microglia (2 × 10 5 cells/well) were treated with E. coli (0.1–100 ng/mL) or V. vulnificus (0.1–10 5 ng/mL) LPS for 17 h in vitro . MIP-2/CXCL2 (Panel A ), MIP-1α/CCL3 (Panel B ), CINC-2α/β/CXCL3 (Panel C ) and MCP-1/CCL2 (Panel D ) release was determined as described in Experimental Section . Data expressed as pg/mL is the mean ± SEM of two to four independent experiments ( n ), each experiment with duplicate determinations. * P

    Article Snippet: Assays for Chemokines: MIP-1α/CCL3, MIP-2/CXCL2, MCP-1/CCL2 and CINC-2α/β/CXCL3 The presence of immunoreactive chemokines in cell-free conditioned media was determined using rat-specific ELISAs: For MIP-1α/CCL3 from Koma Biotech, Seoul, South Korea; for MIP-2/CXCL2 and MCP-1/CCL2 from Biosource International, Camarillo, CA, USA, and for CINC-2α/β/CXCL3 from R & D Systems, Minneapolis, MN, USA.

    Techniques: In Vitro