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  • 95
    Qiagen minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 16148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen minelute 96 uf pcr purification plates
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute 96 Uf Pcr Purification Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen minelute qiaquick pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen minelute reaction cleanup kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Reaction Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2536 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen minelutetm gel extraction kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelutetm Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen hind iii hf minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Hind Iii Hf Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rneasy minelute cleanup kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Rneasy Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 8098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer pb
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Buffer Pb, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaamp minelute virus kit
    H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the <t>QIAamp</t> <t>MinElute</t> Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.
    Qiaamp Minelute Virus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen eb elution buffer
    H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the <t>QIAamp</t> <t>MinElute</t> Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.
    Eb Elution Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaamp minelute media kit
    H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the <t>QIAamp</t> <t>MinElute</t> Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.
    Qiaamp Minelute Media Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaquick gel extraction kit
    H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the <t>QIAamp</t> <t>MinElute</t> Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.
    Qiaquick Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 85963 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaamp minelute virus vacuum
    H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the <t>QIAamp</t> <t>MinElute</t> Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.
    Qiaamp Minelute Virus Vacuum, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the QIAamp MinElute Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.

    Journal: EMBO Molecular Medicine

    Article Title: Synergistic combination of valproic acid and oncolytic parvovirus H-1PV as a potential therapy against cervical and pancreatic carcinomas

    doi: 10.1002/emmm.201302796

    Figure Lengend Snippet: H-1PV/VPA co-treatment leads to complete remission of established HeLa tumours Source data is available for this figure in the Supporting Information. Analysis of tumour growth. Animals ( n = 8 per group) were treated with either PBS (control), VPA or a combination of both agents as described in Materials and Methods Section. Other animal groups are presented in Supporting Information Fig S7 . The data shown represent average tumour volumes with standard deviation bars. Graphical summary of Kaplan–Meier survival analysis of tumour-bearing nude rats treated as indicated. Animals were sacrificed when the tumour reached the maximum tolerable size of 4000 mm 3 according to law. The difference between H-1PV/VPA co-treatment and treatment with H-1PV alone was statistically significant ( p = 0.0002 as calculated with the two-sided log-rank test and adjusted for multiple testing with the Bonferroni method). Other results from the statistical analysis are shown in Supporting Information Table S2. H-1PV/VPA co-treatment induces DNA damage and apoptosis in vivo . Two rats of each treatment group were sacrificed on day 19. Tumours were fixed, sectioned and examined morphologically either by H E staining or by immunochemistry using antibodies against NS1, VP capsid proteins, γ-H2AX (a DNA damage marker), or active cleaved caspase-3 (an apoptosis marker). Scale bar = 50 µm. Higher levels of NS1, VP1 and VP2 viral proteins in tumour samples from VPA co-treated animals. Tumour-bearing animals ( n = 3) treated with H-1PV alone (total virus dose of 3.6 × 10 9 Vg/animal corresponding to 1.5 × 10 8 pfu/animals subdivided into two subdoses given at days 0 and 7) or co-treated with H-1PV and VPA (100 mg/kg) were sacrificed at day 14. Complete tumours were resected, homogenised and lysed. 20 µg of total cellular extracts were analysed by SDS–PAGE for the presence of the viral proteins. Actin was used as a loading control. VPA-enhanced virus multiplication in vivo . Viral particles were recovered from aliquots of tumour extracts from animals treated as in panel D, purified by using the QIAamp MinElute Virus Spin Kit (Qiagen) and quantified by real time qPCR. Columns represent the average amounts of the virus recovered from the tumours of three animals with standard deviation bars. Numbers on top of the columns indicate the fold increase in virus titers in the presence versus absence of VPA.

    Article Snippet: Viral genomic DNA was purified from cell lysates by using the QiaAmp MinElute virus kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Techniques: Standard Deviation, In Vivo, Staining, Marker, SDS Page, Purification, Real-time Polymerase Chain Reaction