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    Qiagen minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 27932 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute pcr purification kit/product/Qiagen
    Average 99 stars, based on 27932 article reviews
    Price from $9.99 to $1999.99
    minelute pcr purification kit - by Bioz Stars, 2020-09
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    99
    Qiagen minelute gel extraction kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 12857 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute gel extraction kit/product/Qiagen
    Average 99 stars, based on 12857 article reviews
    Price from $9.99 to $1999.99
    minelute gel extraction kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen minelute 96 uf pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute 96 Uf Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute 96 uf pcr purification kit/product/Qiagen
    Average 99 stars, based on 1086 article reviews
    Price from $9.99 to $1999.99
    minelute 96 uf pcr purification kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy minelute cleanup kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Rneasy Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 10584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minelute cleanup kit/product/Qiagen
    Average 99 stars, based on 10584 article reviews
    Price from $9.99 to $1999.99
    rneasy minelute cleanup kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen minelute reaction cleanup kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Reaction Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute reaction cleanup kit/product/Qiagen
    Average 99 stars, based on 3435 article reviews
    Price from $9.99 to $1999.99
    minelute reaction cleanup kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    ARv567es binds canonical AREs through a dimerization-dependent mechanism. ( A ) ARE point mutations introduced in FASN ARBSI-LUC. ( B ) Activities of constructs illustrated in (A) were tested in R1-AD1 and R1-D567 cells by luciferase assay. Cells were treated with 1 nM mibolerone (MIB) or ethanol (ETH) as vehicle control as indicated. ( C ) ARE point mutations introduced in TSC2 exon 37-LUC. ( D ) Activities of constructs illustrated in (C) were evaluated by luciferase assay as in (B). ( E ) Western blot of lysates from R1-AD1 cells transfected with HA-GFP and HA-AR-V7 for the ChIP experiment shown in (F). ( F ) Constitutive recruitment of HA-tagged AR-V7 to the FASN ARBS1 site in transfected R1-AD1 cells was tested by ChIP-PCR. Data represent fold enrichment of PCR signal in ChIP DNA isolated using an HA-directed antibody versus non-specific IgG control (which was arbitrarily set to 1). ( G ) Activities of constructs illustrated in (A) were tested by luciferase assay using LNCaP cells transfected with an ARv567es expression vector and treated with 1 nM mibolerone (MIB) or ethanol (ETH, vehicle) as indicated. ( H ) Activities of constructs illustrated in (A) were tested by luciferase assay using LNCaP cells transfected with an AR-V7 expression vector exactly as described in (G). ( I ) Transcriptional activities of wild-type and A596T/S597T D-box mutant versions of ARv567es and AR-V7 were tested in LNCaP cells by luciferase assay as described in (G). ( J ) DNA duplex pull-down assays were performed by incubating biotinylated FASN AREI DNA duplexes harboring core sequences shown in A with cellular extracts from R1-AD1 and R1-D567 cells.

    Journal: Nucleic Acids Research

    Article Title: Targeting chromatin binding regulation of constitutively active AR variants to overcome prostate cancer resistance to endocrine-based therapies

    doi: 10.1093/nar/gkv262

    Figure Lengend Snippet: ARv567es binds canonical AREs through a dimerization-dependent mechanism. ( A ) ARE point mutations introduced in FASN ARBSI-LUC. ( B ) Activities of constructs illustrated in (A) were tested in R1-AD1 and R1-D567 cells by luciferase assay. Cells were treated with 1 nM mibolerone (MIB) or ethanol (ETH) as vehicle control as indicated. ( C ) ARE point mutations introduced in TSC2 exon 37-LUC. ( D ) Activities of constructs illustrated in (C) were evaluated by luciferase assay as in (B). ( E ) Western blot of lysates from R1-AD1 cells transfected with HA-GFP and HA-AR-V7 for the ChIP experiment shown in (F). ( F ) Constitutive recruitment of HA-tagged AR-V7 to the FASN ARBS1 site in transfected R1-AD1 cells was tested by ChIP-PCR. Data represent fold enrichment of PCR signal in ChIP DNA isolated using an HA-directed antibody versus non-specific IgG control (which was arbitrarily set to 1). ( G ) Activities of constructs illustrated in (A) were tested by luciferase assay using LNCaP cells transfected with an ARv567es expression vector and treated with 1 nM mibolerone (MIB) or ethanol (ETH, vehicle) as indicated. ( H ) Activities of constructs illustrated in (A) were tested by luciferase assay using LNCaP cells transfected with an AR-V7 expression vector exactly as described in (G). ( I ) Transcriptional activities of wild-type and A596T/S597T D-box mutant versions of ARv567es and AR-V7 were tested in LNCaP cells by luciferase assay as described in (G). ( J ) DNA duplex pull-down assays were performed by incubating biotinylated FASN AREI DNA duplexes harboring core sequences shown in A with cellular extracts from R1-AD1 and R1-D567 cells.

    Article Snippet: DNA was purified using a PCR purification kit (Qiagen).

    Techniques: Construct, Luciferase, Western Blot, Transfection, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Isolation, Expressing, Plasmid Preparation, Mutagenesis

    Multiplex HPV PCR versus the standard Linear Array with Qiagen MinElute media kit DNA extraction.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿ ‡

    doi: 10.1128/JCM.00235-10

    Figure Lengend Snippet: Multiplex HPV PCR versus the standard Linear Array with Qiagen MinElute media kit DNA extraction.

    Article Snippet: Also shown in , a comparison was conducted on the subset of specimens from which DNA was extracted by the Qiagen MinElute kit in the multiplex HPV PCR and s-LA assays.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, DNA Extraction

    Increased H3 turnover in heterochromatin mutants is not solely due to changes in RNAPII transcription ( a ) Histone H3 replacement (blue) was measured across the mating type locus using MNase–ChIP–on–Chip analysis as described in Fig 1 . RNAPII occupancy (ChIP vs Input) was measured by ChIP–on–Chip in clr3 Δ, clr4 Δ or wild–type (WT) cells and plotted in alignment with the map (red). ( b ) RT–PCR analysis performed using total RNA samples isolated from clr3 Δ, clr4 Δ or wild–type (WT) cells. Genomic DNA (gDNA) was used as a control. The locations amplified by primer pairs 49, 51, 65 and 70 are highlighted with red shading (see online methods for primer references). Heterochromatin and euchromatin portions of the mating–type region are indicated at the top.

    Journal: Nature structural & molecular biology

    Article Title: HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin

    doi: 10.1038/nsmb.2565

    Figure Lengend Snippet: Increased H3 turnover in heterochromatin mutants is not solely due to changes in RNAPII transcription ( a ) Histone H3 replacement (blue) was measured across the mating type locus using MNase–ChIP–on–Chip analysis as described in Fig 1 . RNAPII occupancy (ChIP vs Input) was measured by ChIP–on–Chip in clr3 Δ, clr4 Δ or wild–type (WT) cells and plotted in alignment with the map (red). ( b ) RT–PCR analysis performed using total RNA samples isolated from clr3 Δ, clr4 Δ or wild–type (WT) cells. Genomic DNA (gDNA) was used as a control. The locations amplified by primer pairs 49, 51, 65 and 70 are highlighted with red shading (see online methods for primer references). Heterochromatin and euchromatin portions of the mating–type region are indicated at the top.

    Article Snippet: Finally, samples were treated with 20μg Proteinase K (Invitrogen) for 1 hour at 37 °C, and DNA was purified using QIAGEN PCR purification kits and spin columns.

    Techniques: Chromatin Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction, Isolation, Amplification

    Clr3–dependent suppression of histone turnover correlates with epigenetic stability of heterochromatin ( a ) ChIP analysis of Clr3 localization of at the silent mat region. Strains expressing Myc tagged Clr3 in K Δ ∷ura4 + ura4–on or ura4–off state were used to perform ChIP. ChIP DNA was analyzed by semi–quantitative competitive PCR using primers that amplify both full–length K Δ ∷ura4 + and endogenous mini– ura4 ( ura4DSE ) as internal control. The relative enrichments were determined by calculating the ratio of the band intensities of [ChIP K Δ ∷ura4 + ÷ ChIP ura4DSE ] ÷ [Input K Δ ∷ura4 + ÷ Input ura4DSE ]. Results were confirmed by quantitative real–time PCR (qPCR). Relative enrichment of K Δ ∷ura4 + was normalized against untagged negative control and the mean enrichment is presented. Error bars represent standard error of the mean calculated from 3 independent biological replicates (n=3) ( b ) H3 replacement was measured in K Δ ∷ura4 + ura4–on or ura4–off cells. The endogenous ura4 + was deleted in the strains used. ( c ) ChIP analysis of H3K9me2 levels at K Δ ∷ura4 + ura4–on cells. Experiments were performed with the same strains used in a . H3K9me levels were confirmed by qPCR and the mean enrichment is presented. Error bars represent standard error of the mean calculated from 4 independent biological replicates (n=4).

    Journal: Nature structural & molecular biology

    Article Title: HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin

    doi: 10.1038/nsmb.2565

    Figure Lengend Snippet: Clr3–dependent suppression of histone turnover correlates with epigenetic stability of heterochromatin ( a ) ChIP analysis of Clr3 localization of at the silent mat region. Strains expressing Myc tagged Clr3 in K Δ ∷ura4 + ura4–on or ura4–off state were used to perform ChIP. ChIP DNA was analyzed by semi–quantitative competitive PCR using primers that amplify both full–length K Δ ∷ura4 + and endogenous mini– ura4 ( ura4DSE ) as internal control. The relative enrichments were determined by calculating the ratio of the band intensities of [ChIP K Δ ∷ura4 + ÷ ChIP ura4DSE ] ÷ [Input K Δ ∷ura4 + ÷ Input ura4DSE ]. Results were confirmed by quantitative real–time PCR (qPCR). Relative enrichment of K Δ ∷ura4 + was normalized against untagged negative control and the mean enrichment is presented. Error bars represent standard error of the mean calculated from 3 independent biological replicates (n=3) ( b ) H3 replacement was measured in K Δ ∷ura4 + ura4–on or ura4–off cells. The endogenous ura4 + was deleted in the strains used. ( c ) ChIP analysis of H3K9me2 levels at K Δ ∷ura4 + ura4–on cells. Experiments were performed with the same strains used in a . H3K9me levels were confirmed by qPCR and the mean enrichment is presented. Error bars represent standard error of the mean calculated from 4 independent biological replicates (n=4).

    Article Snippet: Finally, samples were treated with 20μg Proteinase K (Invitrogen) for 1 hour at 37 °C, and DNA was purified using QIAGEN PCR purification kits and spin columns.

    Techniques: Chromatin Immunoprecipitation, Expressing, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Negative Control

    Figure 5. Nested PCR products of 345bp assessed by electrophoresis in a 1.2% Agarose gel. L = 100bp DNA ladder (Promega, UK), (2–6) CS-scars, (7) Positive control using placental tissue and (8) Negative control using nulliparous skin.

    Journal: Chimerism

    Article Title: Microchimeric fetal cells play a role in maternal wound healing after pregnancy

    doi: 10.4161/chim.28746

    Figure Lengend Snippet: Figure 5. Nested PCR products of 345bp assessed by electrophoresis in a 1.2% Agarose gel. L = 100bp DNA ladder (Promega, UK), (2–6) CS-scars, (7) Positive control using placental tissue and (8) Negative control using nulliparous skin.

    Article Snippet: The PCR product generated after the first reaction was then column purified using the Min Elute PCR purification kit (Qiagen) and DNA was eluted using 40 µl of DEPC-treated water.

    Techniques: Nested PCR, Electrophoresis, Agarose Gel Electrophoresis, Positive Control, Negative Control

    The toxin profiles and PCR ribotyping characters of C. difficile between children and adults in this study. ( A ) The toxin profile of isolated strains. ( B ) RT constituent ratio of C. difficile strains. Left: children group; right: adults group. ( C ) RT distribu tion characters of C. difficile for different hospitals in children group. ( D ) RT distribution characters of C. difficile for different hospitals in adults group. ( E ). RT distribution characters of C. difficile for fecal property in children group. ( F ) RT distribution characters of C. difficile for fecal property in adults group.

    Journal: Scientific Reports

    Article Title: A retrospective study of community-acquired Clostridium difficile infection in southwest China

    doi: 10.1038/s41598-018-21762-7

    Figure Lengend Snippet: The toxin profiles and PCR ribotyping characters of C. difficile between children and adults in this study. ( A ) The toxin profile of isolated strains. ( B ) RT constituent ratio of C. difficile strains. Left: children group; right: adults group. ( C ) RT distribu tion characters of C. difficile for different hospitals in children group. ( D ) RT distribution characters of C. difficile for different hospitals in adults group. ( E ). RT distribution characters of C. difficile for fecal property in children group. ( F ) RT distribution characters of C. difficile for fecal property in adults group.

    Article Snippet: PCR ribotyping reaction products were concentrated using a Qiagen Min-Elute PCR purification kit (QIAGEN) and run on a QIAxcel capillary electrophoresis platform (QIAGEN).

    Techniques: Polymerase Chain Reaction, Isolation