middlebrook adc Search Results


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  • 99
    Millipore adc growth supplement
    Adc Growth Supplement, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook adc
    Middlebrook Adc, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals adc middlebrook
    Adc Middlebrook, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher middlebrook adc
    Middlebrook Adc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook adc
    Middlebrook Adc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor middlebrook adc
    Middlebrook Adc, supplied by Avantor, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MiddleBrook Pharmaceuticals 7h9 adc middlebrook
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    7h9 Adc Middlebrook, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco mo middlebrook adc enrichment
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Mo Middlebrook Adc Enrichment, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals adc middlebrook supplement
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Adc Middlebrook Supplement, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals bacto middlebrook adc
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Bacto Middlebrook Adc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals bbl middlebrook adc enrichment
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Bbl Middlebrook Adc Enrichment, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals adc supplemented middlebrook 7h9
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Adc Supplemented Middlebrook 7h9, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals sterile middlebrook adc enrichment
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Sterile Middlebrook Adc Enrichment, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals additive middlebrook adc enrichment
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Additive Middlebrook Adc Enrichment, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    MiddleBrook Pharmaceuticals middlebrook 7h10 adc
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Middlebrook 7h10 Adc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals bacto middlebrook adc enrichment
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Bacto Middlebrook Adc Enrichment, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals liquid middlebrook 7h9 adc
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Liquid Middlebrook 7h9 Adc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 adc broth
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Middlebrook 7h9 Adc Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 adc oadc
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Middlebrook 7h9 Adc Oadc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h11 adc
    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid <t>Middlebrook</t> <t>7H9</t> medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook <t>7H9–ADC</t> medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.
    Middlebrook 7h11 Adc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 adc medium
    Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in <t>Middlebrook</t> <t>7H9</t> in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.
    Middlebrook 7h9 Adc Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals enrichment medium middlebrook 7h9 adc middlebrook
    Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in <t>Middlebrook</t> <t>7H9</t> in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.
    Enrichment Medium Middlebrook 7h9 Adc Middlebrook, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals bbl middlebrook adc enrichment broth
    Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in <t>Middlebrook</t> <t>7H9</t> in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.
    Bbl Middlebrook Adc Enrichment Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals adc supplemented middlebrook 7h9 broth
    Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in <t>Middlebrook</t> <t>7H9</t> in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.
    Adc Supplemented Middlebrook 7h9 Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 adc tween
    In vitro growth curves. (A) Growth of wild-type H37Rv (wt) (■), the Δ rel mutant (▴), and the complemented strain C7 (●) in <t>7H9</t> medium at 37°C, determined by OD 650 . Panel B is the same, but at 42°C. (C) Growth at 37°C in 7H9 with the carbon sources of glucose and glycerol replaced with 0.2 mM DPPC.
    Middlebrook 7h9 Adc Tween, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 middlebrook adc tween 80
    In vitro growth curves. (A) Growth of wild-type H37Rv (wt) (■), the Δ rel mutant (▴), and the complemented strain C7 (●) in <t>7H9</t> medium at 37°C, determined by OD 650 . Panel B is the same, but at 42°C. (C) Growth at 37°C in 7H9 with the carbon sources of glucose and glycerol replaced with 0.2 mM DPPC.
    7h9 Middlebrook Adc Tween 80, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook albumin dextrose catalase adc difco
    In vitro growth curves. (A) Growth of wild-type H37Rv (wt) (■), the Δ rel mutant (▴), and the complemented strain C7 (●) in <t>7H9</t> medium at 37°C, determined by OD 650 . Panel B is the same, but at 42°C. (C) Growth at 37°C in 7H9 with the carbon sources of glucose and glycerol replaced with 0.2 mM DPPC.
    Middlebrook Albumin Dextrose Catalase Adc Difco, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals adc supplemented middlebrook 7h9 medium
    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in <t>ADC-supplemented</t> <t>Middlebrook</t> <t>7H9</t> medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P
    Adc Supplemented Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h10 adc medium
    Construction and Characterization of MTBVACΔ lys Strain (A) lysA gene (gray arrow) from MTBVAC was inactivated using homologous recombination techniques by introducing a kanamycin resistance cassette (gray rectangle), flanked by two resolvase sites (white arrowheads) in order to allow the release of the resistance cassette. The inactivated phoP and fadD2 6 genes in the MTBVAC parental strain are also illustrated. (B) Genotypic characterization of the lysA gene inactivation by the kanamycin cassette (km) insertion, primers used, and expected sizes of the PCR products are indicated. MTBVAC sample was used in lanes 3, 5, and 7 and MTBVACΔ lys samples in lanes 4, 6, and 8. Lane 1, molecular weight marker; lane 2, negative control; lanes 3 and 4, PCR product of the lysA gene using Lys-fw and Lys-rv primers; lanes 5 and 6, PCR of the 5′ insertion point of km expression cassette using Lys-fw and km-OUT-rv primers; and lanes 7 and 8, PCR of the 3′ insertion point of km expression cassette using km-OUT-fw and Lys-rv primers. Genes are represented as gray arrows; gray rectangles illustrate antibiotic resistance markers and white arrowheads depict resolvase recognition sequences or res sites. (C) Phenotypic lysine auxotrophic verification by plating MTBVACΔ lys strain in <t>7H10-ADC</t> with and without lysine supplementation.
    Middlebrook 7h10 Adc Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h10 adc
    Construction and Characterization of MTBVACΔ lys Strain (A) lysA gene (gray arrow) from MTBVAC was inactivated using homologous recombination techniques by introducing a kanamycin resistance cassette (gray rectangle), flanked by two resolvase sites (white arrowheads) in order to allow the release of the resistance cassette. The inactivated phoP and fadD2 6 genes in the MTBVAC parental strain are also illustrated. (B) Genotypic characterization of the lysA gene inactivation by the kanamycin cassette (km) insertion, primers used, and expected sizes of the PCR products are indicated. MTBVAC sample was used in lanes 3, 5, and 7 and MTBVACΔ lys samples in lanes 4, 6, and 8. Lane 1, molecular weight marker; lane 2, negative control; lanes 3 and 4, PCR product of the lysA gene using Lys-fw and Lys-rv primers; lanes 5 and 6, PCR of the 5′ insertion point of km expression cassette using Lys-fw and km-OUT-rv primers; and lanes 7 and 8, PCR of the 3′ insertion point of km expression cassette using km-OUT-fw and Lys-rv primers. Genes are represented as gray arrows; gray rectangles illustrate antibiotic resistance markers and white arrowheads depict resolvase recognition sequences or res sites. (C) Phenotypic lysine auxotrophic verification by plating MTBVACΔ lys strain in <t>7H10-ADC</t> with and without lysine supplementation.
    7h10 Adc, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals fresh middlebrook 7h9 adc tween 80
    Construction and Characterization of MTBVACΔ lys Strain (A) lysA gene (gray arrow) from MTBVAC was inactivated using homologous recombination techniques by introducing a kanamycin resistance cassette (gray rectangle), flanked by two resolvase sites (white arrowheads) in order to allow the release of the resistance cassette. The inactivated phoP and fadD2 6 genes in the MTBVAC parental strain are also illustrated. (B) Genotypic characterization of the lysA gene inactivation by the kanamycin cassette (km) insertion, primers used, and expected sizes of the PCR products are indicated. MTBVAC sample was used in lanes 3, 5, and 7 and MTBVACΔ lys samples in lanes 4, 6, and 8. Lane 1, molecular weight marker; lane 2, negative control; lanes 3 and 4, PCR product of the lysA gene using Lys-fw and Lys-rv primers; lanes 5 and 6, PCR of the 5′ insertion point of km expression cassette using Lys-fw and km-OUT-rv primers; and lanes 7 and 8, PCR of the 3′ insertion point of km expression cassette using km-OUT-fw and Lys-rv primers. Genes are represented as gray arrows; gray rectangles illustrate antibiotic resistance markers and white arrowheads depict resolvase recognition sequences or res sites. (C) Phenotypic lysine auxotrophic verification by plating MTBVACΔ lys strain in <t>7H10-ADC</t> with and without lysine supplementation.
    Fresh Middlebrook 7h9 Adc Tween 80, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid Middlebrook 7H9 medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook 7H9–ADC medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.

    Journal: Infection and Immunity

    Article Title: Disruption of the Genes Encoding Antigen 85A and Antigen 85B of Mycobacterium tuberculosis H37Rv: Effect on Growth in Culture and in Macrophages

    doi:

    Figure Lengend Snippet: Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid Middlebrook 7H9 medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook 7H9–ADC medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.

    Article Snippet: The parent strain, H37Rv, and the fbpA and fbpB mutants were washed with saline and added at 104 CFU/ml to the enriched Middlebrook 7H9–ADC and synthetic Sauton media and incubated with gentle mixing at 37°C for 14 days.

    Techniques: Mutagenesis

    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified 7H9/ADC [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.

    Journal: Infection and Immunity

    Article Title: A Mutation in the 16S rRNA Decoding Region Attenuates the Virulence of Mycobacterium tuberculosis

    doi: 10.1128/IAI.00417-16

    Figure Lengend Snippet: Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified 7H9/ADC [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.

    Article Snippet: The growth speeds of NCGM2242 and NCGM2242(pMV261Zeo) (mock) were identical in 7H9/ADC.

    Techniques: Modification

    Complementation by the wild-type rRNA operon. (A) Growth curves of strains Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT) in 7H9/ADC medium. Data are means ± standard deviations of results from three independent experiments. (B) Expression of KsgA in Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT). M. tuberculosis strains were cultured in 7H9/ADC at 37°C for 4 days with shaking. Lysates of the indicated strains were immunoblotted with anti-KsgA antibody. Asterisks indicate nonspecific bands. Arrows indicate endogenous KsgA. (C) Bacterial loads at day 94 in the spleens, livers, and lungs of BALB/c mice i nfected with strain Erdman ( n = 6), NCGM2242(pMV261Zeo-rrsWT) ( n = 6), or NCGM2242(pMV261Zeo) ( n = 5). *, P

    Journal: Infection and Immunity

    Article Title: A Mutation in the 16S rRNA Decoding Region Attenuates the Virulence of Mycobacterium tuberculosis

    doi: 10.1128/IAI.00417-16

    Figure Lengend Snippet: Complementation by the wild-type rRNA operon. (A) Growth curves of strains Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT) in 7H9/ADC medium. Data are means ± standard deviations of results from three independent experiments. (B) Expression of KsgA in Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT). M. tuberculosis strains were cultured in 7H9/ADC at 37°C for 4 days with shaking. Lysates of the indicated strains were immunoblotted with anti-KsgA antibody. Asterisks indicate nonspecific bands. Arrows indicate endogenous KsgA. (C) Bacterial loads at day 94 in the spleens, livers, and lungs of BALB/c mice i nfected with strain Erdman ( n = 6), NCGM2242(pMV261Zeo-rrsWT) ( n = 6), or NCGM2242(pMV261Zeo) ( n = 5). *, P

    Article Snippet: The growth speeds of NCGM2242 and NCGM2242(pMV261Zeo) (mock) were identical in 7H9/ADC.

    Techniques: Expressing, Cell Culture, Mouse Assay

    Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in Middlebrook 7H9 in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.

    Journal: Infection and Immunity

    Article Title: A Peptide Permease Mutant of Mycobacterium bovis BCG Resistant to the Toxic Peptides Glutathione and S-Nitrosoglutathione

    doi:

    Figure Lengend Snippet: Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in Middlebrook 7H9 in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.

    Article Snippet: Cells growing in Middlebrook 7H9 (plus glycerol, ADC, and 0.05% Tween) were collected at an OD600 of 0.6, washed in basal salts with 0.05% Tween, and concentrated to an OD600 of 3.0.

    Techniques: Inhibition, Incubation

    Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid Middlebrook 7H9 medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook 7H9–ADC medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.

    Journal: Infection and Immunity

    Article Title: Disruption of the Genes Encoding Antigen 85A and Antigen 85B of Mycobacterium tuberculosis H37Rv: Effect on Growth in Culture and in Macrophages

    doi:

    Figure Lengend Snippet: Effect of medium composition on the growth of fbpA - and fbpB -deficient M. tuberculosis mutants. Saline-washed M. tuberculosis H37Rv or an fbpA or fbpB mutant was inoculated into enriched liquid Middlebrook 7H9 medium or wholly synthetic Sauton medium. All strains grew similarly in the enriched Middlebrook 7H9–ADC medium. The fbpA mutant, LAa1, was unable to grow in the Sauton medium, which lacks the ADC supplement.

    Article Snippet: While all three strains grew in the Middlebrook 7H9–ADC broth at very similar rates, the fbpA mutant, LAa1, was unable to grow in the synthetic Sauton medium (Fig. ).

    Techniques: Mutagenesis

    Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in Middlebrook 7H9 in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.

    Journal: Infection and Immunity

    Article Title: A Peptide Permease Mutant of Mycobacterium bovis BCG Resistant to the Toxic Peptides Glutathione and S-Nitrosoglutathione

    doi:

    Figure Lengend Snippet: Inhibition of growth of wild-type and ( opp Δ-19) BCG by GSH. Exponentially growing cells (OD 600 , 0.4 to 0.6) were diluted to an OD 600 of 0.1 and incubated in 24-well plates in Middlebrook 7H9 in the presence or absence of GSH at the concentrations indicated. After 3 days, the OD 600 of the cultures was measured. Black bars, wild-type BCG; hatched bars, BCG( opp Δ-19). The experiment is representative of three similar experiments.

    Article Snippet: To analyze the effects of GSH on BCG, cultures of wild-type cells growing in Middlebrook 7H9 medium (with glycerol, ADC, and 0.05% Tween) were treated with increasing concentrations of GSH for 3 days.

    Techniques: Inhibition, Incubation

    In vitro growth curves. (A) Growth of wild-type H37Rv (wt) (■), the Δ rel mutant (▴), and the complemented strain C7 (●) in 7H9 medium at 37°C, determined by OD 650 . Panel B is the same, but at 42°C. (C) Growth at 37°C in 7H9 with the carbon sources of glucose and glycerol replaced with 0.2 mM DPPC.

    Journal: Journal of Bacteriology

    Article Title: The Stringent Response of Mycobacterium tuberculosis Is Required for Long-Term Survival

    doi:

    Figure Lengend Snippet: In vitro growth curves. (A) Growth of wild-type H37Rv (wt) (■), the Δ rel mutant (▴), and the complemented strain C7 (●) in 7H9 medium at 37°C, determined by OD 650 . Panel B is the same, but at 42°C. (C) Growth at 37°C in 7H9 with the carbon sources of glucose and glycerol replaced with 0.2 mM DPPC.

    Article Snippet: M. tuberculosis strains were grown in 7H9-ADC-Tween as the reference media.

    Techniques: In Vitro, Mutagenesis

    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in ADC-supplemented Middlebrook 7H9 medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P

    Journal: Frontiers in Microbiology

    Article Title: Interfering With DNA Decondensation as a Strategy Against Mycobacteria

    doi: 10.3389/fmicb.2018.02034

    Figure Lengend Snippet: M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in ADC-supplemented Middlebrook 7H9 medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P

    Article Snippet: M. smegmatis was cultured in ADC-supplemented Middlebrook 7H9 medium before being transferred to PBS.

    Techniques: Cell Culture

    Construction and Characterization of MTBVACΔ lys Strain (A) lysA gene (gray arrow) from MTBVAC was inactivated using homologous recombination techniques by introducing a kanamycin resistance cassette (gray rectangle), flanked by two resolvase sites (white arrowheads) in order to allow the release of the resistance cassette. The inactivated phoP and fadD2 6 genes in the MTBVAC parental strain are also illustrated. (B) Genotypic characterization of the lysA gene inactivation by the kanamycin cassette (km) insertion, primers used, and expected sizes of the PCR products are indicated. MTBVAC sample was used in lanes 3, 5, and 7 and MTBVACΔ lys samples in lanes 4, 6, and 8. Lane 1, molecular weight marker; lane 2, negative control; lanes 3 and 4, PCR product of the lysA gene using Lys-fw and Lys-rv primers; lanes 5 and 6, PCR of the 5′ insertion point of km expression cassette using Lys-fw and km-OUT-rv primers; and lanes 7 and 8, PCR of the 3′ insertion point of km expression cassette using km-OUT-fw and Lys-rv primers. Genes are represented as gray arrows; gray rectangles illustrate antibiotic resistance markers and white arrowheads depict resolvase recognition sequences or res sites. (C) Phenotypic lysine auxotrophic verification by plating MTBVACΔ lys strain in 7H10-ADC with and without lysine supplementation.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: MTBVAC-Based TB-HIV Vaccine Is Safe, Elicits HIV-T Cell Responses, and Protects against Mycobacterium tuberculosis in Mice

    doi: 10.1016/j.omtm.2019.01.014

    Figure Lengend Snippet: Construction and Characterization of MTBVACΔ lys Strain (A) lysA gene (gray arrow) from MTBVAC was inactivated using homologous recombination techniques by introducing a kanamycin resistance cassette (gray rectangle), flanked by two resolvase sites (white arrowheads) in order to allow the release of the resistance cassette. The inactivated phoP and fadD2 6 genes in the MTBVAC parental strain are also illustrated. (B) Genotypic characterization of the lysA gene inactivation by the kanamycin cassette (km) insertion, primers used, and expected sizes of the PCR products are indicated. MTBVAC sample was used in lanes 3, 5, and 7 and MTBVACΔ lys samples in lanes 4, 6, and 8. Lane 1, molecular weight marker; lane 2, negative control; lanes 3 and 4, PCR product of the lysA gene using Lys-fw and Lys-rv primers; lanes 5 and 6, PCR of the 5′ insertion point of km expression cassette using Lys-fw and km-OUT-rv primers; and lanes 7 and 8, PCR of the 3′ insertion point of km expression cassette using km-OUT-fw and Lys-rv primers. Genes are represented as gray arrows; gray rectangles illustrate antibiotic resistance markers and white arrowheads depict resolvase recognition sequences or res sites. (C) Phenotypic lysine auxotrophic verification by plating MTBVACΔ lys strain in 7H10-ADC with and without lysine supplementation.

    Article Snippet: Electrocompetent MTBVACΔlys was transformed with 0.5–1 μg p2auxo.HIVA or p2auxo.∅, using a Bio-Rad gene pulser electroporator at 2.5 kV, 25 mF, and 1,000 Ω, and plated onto Middlebrook agar 7H10-ADC medium without lysine supplementation.

    Techniques: Homologous Recombination, Polymerase Chain Reaction, Molecular Weight, Marker, Negative Control, Expressing