middlebrook 7h9 medium Search Results


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  • 99
    Thermo Fisher middlebrook 7h9 medium
    Disruption of pknG or removal of the phosphorylation motif of garA caused a nutrient-dependent growth defect in M . smegmatis . ( A ) All strains grew at the same rate on standard <t>Middlebrook</t> <t>7H9</t> medium. ( B ) Δ garA Ms grew slower than wild type on minimal Sauton’s medium containing 20 mM propionate, 20 mM NH 4 Cl plus 0.05% tyloxapol, and this growth defect could be fully complemented by GarA lacking phosphorylation sites (truncated “trunc.” garA ). ( C + D ) Δ pknG Ms grew slower than wild type and formed clumps (inset photo) on medium containing glutamate as sole carbon ( C ) or nitrogen source ( D ) (minimal Sauton’s with either 30 mM glutamate plus tyloxapol, or 1% glycerol, 10 mM glutamate plus tyloxapol). Data plotted are the mean and standard deviation of at least 3 independent experiments. ( E ) Δ pknG Ms formed clumps when glutamate was the sole carbon or nitrogen source. The photograph shows a microplate from growth curve ( D ) imaged at 60 hours. Growth of Δ garA Ms complemented with phosphoablative GarA (EAAS) was equivalent to that of Δ pknG Ms complemented with truncated GarA in all tested conditions so only the dataset for truncated GarA is shown for clarity.
    Middlebrook 7h9 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 7h9 medium
    Disruption of pknG or removal of the phosphorylation motif of garA caused a nutrient-dependent growth defect in M . smegmatis . ( A ) All strains grew at the same rate on standard <t>Middlebrook</t> <t>7H9</t> medium. ( B ) Δ garA Ms grew slower than wild type on minimal Sauton’s medium containing 20 mM propionate, 20 mM NH 4 Cl plus 0.05% tyloxapol, and this growth defect could be fully complemented by GarA lacking phosphorylation sites (truncated “trunc.” garA ). ( C + D ) Δ pknG Ms grew slower than wild type and formed clumps (inset photo) on medium containing glutamate as sole carbon ( C ) or nitrogen source ( D ) (minimal Sauton’s with either 30 mM glutamate plus tyloxapol, or 1% glycerol, 10 mM glutamate plus tyloxapol). Data plotted are the mean and standard deviation of at least 3 independent experiments. ( E ) Δ pknG Ms formed clumps when glutamate was the sole carbon or nitrogen source. The photograph shows a microplate from growth curve ( D ) imaged at 60 hours. Growth of Δ garA Ms complemented with phosphoablative GarA (EAAS) was equivalent to that of Δ pknG Ms complemented with truncated GarA in all tested conditions so only the dataset for truncated GarA is shown for clarity.
    7h9 Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h9 medium
    Cell surface of Msmeg-PE11 is highly hydrophobic. ( a ) Pellicle formation of Msmeg-pVV or Msmeg-PE11 was monitored by growing standing cultures of the strains without shaking in <t>Middlebrook</t> <t>7H9</t> medium in absence of Tween 80 at 37 °C for various time points. ( b ) Biofilm formation was quantified by crystal violet staining for which Msmeg-pVV or Msmeg-PE11 cells were washed, stained with 1% crystal violet and ethanol extract was spectrophotometrically measured at 570 nm. ( c ) Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 medium with (upper panel) or without (lower panel) 0.05% Tween-80 at 37 °C with shaking for 24 h and 48 h. Cultures were then allowed to settle at room temperature for 30 min. ( d ) In another experiment, Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 broth with congo red (100 μg/ml) and 0.05% Tween 80 for 24 h, 48 h and 72 h at 37 °C. Cells were next washed and re-suspended in acetone. Congo red in the supernatant was spectrophotometrically measured at 490 nm. Data are representative of mean ± SD of three different experiments.
    Middlebrook 7h9 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co middlebrook 7h9 medium
    Cell surface of Msmeg-PE11 is highly hydrophobic. ( a ) Pellicle formation of Msmeg-pVV or Msmeg-PE11 was monitored by growing standing cultures of the strains without shaking in <t>Middlebrook</t> <t>7H9</t> medium in absence of Tween 80 at 37 °C for various time points. ( b ) Biofilm formation was quantified by crystal violet staining for which Msmeg-pVV or Msmeg-PE11 cells were washed, stained with 1% crystal violet and ethanol extract was spectrophotometrically measured at 570 nm. ( c ) Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 medium with (upper panel) or without (lower panel) 0.05% Tween-80 at 37 °C with shaking for 24 h and 48 h. Cultures were then allowed to settle at room temperature for 30 min. ( d ) In another experiment, Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 broth with congo red (100 μg/ml) and 0.05% Tween 80 for 24 h, 48 h and 72 h at 37 °C. Cells were next washed and re-suspended in acetone. Congo red in the supernatant was spectrophotometrically measured at 490 nm. Data are representative of mean ± SD of three different experiments.
    Middlebrook 7h9 Medium, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLife Solutions middlebrook 7h9 medium
    Cell surface of Msmeg-PE11 is highly hydrophobic. ( a ) Pellicle formation of Msmeg-pVV or Msmeg-PE11 was monitored by growing standing cultures of the strains without shaking in <t>Middlebrook</t> <t>7H9</t> medium in absence of Tween 80 at 37 °C for various time points. ( b ) Biofilm formation was quantified by crystal violet staining for which Msmeg-pVV or Msmeg-PE11 cells were washed, stained with 1% crystal violet and ethanol extract was spectrophotometrically measured at 570 nm. ( c ) Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 medium with (upper panel) or without (lower panel) 0.05% Tween-80 at 37 °C with shaking for 24 h and 48 h. Cultures were then allowed to settle at room temperature for 30 min. ( d ) In another experiment, Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 broth with congo red (100 μg/ml) and 0.05% Tween 80 for 24 h, 48 h and 72 h at 37 °C. Cells were next washed and re-suspended in acetone. Congo red in the supernatant was spectrophotometrically measured at 490 nm. Data are representative of mean ± SD of three different experiments.
    Middlebrook 7h9 Medium, supplied by BioLife Solutions, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middlebrook 7h9 medium
    Cell surface of Msmeg-PE11 is highly hydrophobic. ( a ) Pellicle formation of Msmeg-pVV or Msmeg-PE11 was monitored by growing standing cultures of the strains without shaking in <t>Middlebrook</t> <t>7H9</t> medium in absence of Tween 80 at 37 °C for various time points. ( b ) Biofilm formation was quantified by crystal violet staining for which Msmeg-pVV or Msmeg-PE11 cells were washed, stained with 1% crystal violet and ethanol extract was spectrophotometrically measured at 570 nm. ( c ) Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 medium with (upper panel) or without (lower panel) 0.05% Tween-80 at 37 °C with shaking for 24 h and 48 h. Cultures were then allowed to settle at room temperature for 30 min. ( d ) In another experiment, Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 broth with congo red (100 μg/ml) and 0.05% Tween 80 for 24 h, 48 h and 72 h at 37 °C. Cells were next washed and re-suspended in acetone. Congo red in the supernatant was spectrophotometrically measured at 490 nm. Data are representative of mean ± SD of three different experiments.
    Middlebrook 7h9 Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 medium
    FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in <t>Middlebrook</t> <t>7H9</t> medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.
    Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 2844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middlebrooks 7h9 liquid medium
    FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in <t>Middlebrook</t> <t>7H9</t> medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.
    Middlebrooks 7h9 Liquid Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 7h9 liquid medium
    Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in <t>7H9</t> complete medium
    7h9 Liquid Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook s 7h9 broth medium
    Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in <t>7H9</t> complete medium
    Middlebrook S 7h9 Broth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor middlebrook 7h9 medium
    Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in <t>7H9</t> complete medium
    Middlebrook 7h9 Medium, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 middlebrook medium
    51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of <t>Middlebrook</t> <t>7H9</t> broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .
    7h9 Middlebrook Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 7h9 middlebrook medium
    51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of <t>Middlebrook</t> <t>7H9</t> broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .
    7h9 Middlebrook Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Difco middlebrooke 7h9 medium
    51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of <t>Middlebrook</t> <t>7H9</t> broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .
    Middlebrooke 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories middlebrook 7h9 medium
    51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of <t>Middlebrook</t> <t>7H9</t> broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .
    Middlebrook 7h9 Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h9
    51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of <t>Middlebrook</t> <t>7H9</t> broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .
    Middlebrook 7h9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals liquid middlebrook 7h9 medium
    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in <t>Middlebrook</t> <t>7H9-Tween</t> medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.
    Liquid Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Middlebrook 7h9, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals fresh middlebrook 7h9 medium
    M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in <t>Middlebrook</t> <t>7H9</t> medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.
    Fresh Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals medium middlebrook 7h9 broth
    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in <t>Middlebrook</t> <t>7H9-Tween</t> medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.
    Medium Middlebrook 7h9 Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 97/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 medium oadc
    The growth curves for M. paratuberculosis JTC114 were obtained from cultures grown in <t>7H9-OADC</t> (A) or WR-GD (B) medium at pHs 6.0 and 6.8. The photographs present surface morphologies in liquid culture flasks of M. paratuberculosis JTC114 at the stationary phase in 7H9-OADC (C) or WR-GD (D) medium at pH 6.0.
    Middlebrook 7h9 Medium Oadc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals mycomedia middlebrook 7h9 medium
    The growth curves for M. paratuberculosis JTC114 were obtained from cultures grown in <t>7H9-OADC</t> (A) or WR-GD (B) medium at pHs 6.0 and 6.8. The photographs present surface morphologies in liquid culture flasks of M. paratuberculosis JTC114 at the stationary phase in 7H9-OADC (C) or WR-GD (D) medium at pH 6.0.
    Mycomedia Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals sterile middlebrook 7h9 medium
    The growth curves for M. paratuberculosis JTC114 were obtained from cultures grown in <t>7H9-OADC</t> (A) or WR-GD (B) medium at pHs 6.0 and 6.8. The photographs present surface morphologies in liquid culture flasks of M. paratuberculosis JTC114 at the stationary phase in 7H9-OADC (C) or WR-GD (D) medium at pH 6.0.
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    The growth curves for M. paratuberculosis JTC114 were obtained from cultures grown in <t>7H9-OADC</t> (A) or WR-GD (B) medium at pHs 6.0 and 6.8. The photographs present surface morphologies in liquid culture flasks of M. paratuberculosis JTC114 at the stationary phase in 7H9-OADC (C) or WR-GD (D) medium at pH 6.0.
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    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in <t>ADC-supplemented</t> <t>Middlebrook</t> <t>7H9</t> medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P
    7h9 Middlebrook Supplemented Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in <t>ADC-supplemented</t> <t>Middlebrook</t> <t>7H9</t> medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P
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    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in <t>ADC-supplemented</t> <t>Middlebrook</t> <t>7H9</t> medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P
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    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in <t>ADC-supplemented</t> <t>Middlebrook</t> <t>7H9</t> medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P
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    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in <t>ADC-supplemented</t> <t>Middlebrook</t> <t>7H9</t> medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P
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    Image Search Results


    Disruption of pknG or removal of the phosphorylation motif of garA caused a nutrient-dependent growth defect in M . smegmatis . ( A ) All strains grew at the same rate on standard Middlebrook 7H9 medium. ( B ) Δ garA Ms grew slower than wild type on minimal Sauton’s medium containing 20 mM propionate, 20 mM NH 4 Cl plus 0.05% tyloxapol, and this growth defect could be fully complemented by GarA lacking phosphorylation sites (truncated “trunc.” garA ). ( C + D ) Δ pknG Ms grew slower than wild type and formed clumps (inset photo) on medium containing glutamate as sole carbon ( C ) or nitrogen source ( D ) (minimal Sauton’s with either 30 mM glutamate plus tyloxapol, or 1% glycerol, 10 mM glutamate plus tyloxapol). Data plotted are the mean and standard deviation of at least 3 independent experiments. ( E ) Δ pknG Ms formed clumps when glutamate was the sole carbon or nitrogen source. The photograph shows a microplate from growth curve ( D ) imaged at 60 hours. Growth of Δ garA Ms complemented with phosphoablative GarA (EAAS) was equivalent to that of Δ pknG Ms complemented with truncated GarA in all tested conditions so only the dataset for truncated GarA is shown for clarity.

    Journal: PLoS Pathogens

    Article Title: PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis

    doi: 10.1371/journal.ppat.1006399

    Figure Lengend Snippet: Disruption of pknG or removal of the phosphorylation motif of garA caused a nutrient-dependent growth defect in M . smegmatis . ( A ) All strains grew at the same rate on standard Middlebrook 7H9 medium. ( B ) Δ garA Ms grew slower than wild type on minimal Sauton’s medium containing 20 mM propionate, 20 mM NH 4 Cl plus 0.05% tyloxapol, and this growth defect could be fully complemented by GarA lacking phosphorylation sites (truncated “trunc.” garA ). ( C + D ) Δ pknG Ms grew slower than wild type and formed clumps (inset photo) on medium containing glutamate as sole carbon ( C ) or nitrogen source ( D ) (minimal Sauton’s with either 30 mM glutamate plus tyloxapol, or 1% glycerol, 10 mM glutamate plus tyloxapol). Data plotted are the mean and standard deviation of at least 3 independent experiments. ( E ) Δ pknG Ms formed clumps when glutamate was the sole carbon or nitrogen source. The photograph shows a microplate from growth curve ( D ) imaged at 60 hours. Growth of Δ garA Ms complemented with phosphoablative GarA (EAAS) was equivalent to that of Δ pknG Ms complemented with truncated GarA in all tested conditions so only the dataset for truncated GarA is shown for clarity.

    Article Snippet: Bacterial strains, media, and culture M . tuberculosis H37Rv and M . smegmatis mc2 155 were routinely cultured on Middlebrook 7H10 agar (Oxoid) with 10% ADN (0.5% bovine serum albumin, 0.2% dextrose, 0.085% NaCl) and Middlebrook 7H9 medium (Oxoid) with 10% ADN and 0.05% Tween 80.

    Techniques: Mass Spectrometry, Standard Deviation

    garA is required for growth of M . tuberculosis in vitro , survival in macrophages, and virulence in mice. ( A ) M . tuberculosis lacking garA was unable to grow on standard 7H10 medium unless supplemented with asparagine. Plasmid-borne garA restored the defect, but variants of garA with mutations at threonine 21 in the phosphorylation motif (ETTS) gave only partial complementation. Strains were grown in Middlebrook 7H9 plus 30 mM asparagine then washed and diluted in standard 7H9 and spotted onto standard 7H10 with or without 10 mM asparagine. Photographs are representative of at least 3 independent experiments. ( B ) M . tuberculosis lacking garA (red squares) had a defect in growth and survival in differentiated THP-1 cells compared to parental M . tuberculosis H37Rv (black circles). Re-introduction of GarA (black triangles) or variants of GarA lacking a single phosphorylation site (grey crosses and squares) restored growth but variant GarA lacking both phosphorylation sites (green triangles) did not. Data points show the mean and standard deviation from four replicates and are representative of two independent experiments. ( C ) M . tuberculosis lacking garA was avirulent in mice as it was eliminated from the lungs. BALB/C mice were infected intranasally with 10 5 bacilli and bacterial burden was measured on days 1, 7, 21 and 28. Data points show the bacterial burden in individual animals. The bacterial burden of mice infected with Δ garA Mt (red squares) was significantly lower than those infected with M . tuberculosis H37Rv (black circles), or complemented Δ garA Mt (black triangles) at all time points from day 7 (p

    Journal: PLoS Pathogens

    Article Title: PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis

    doi: 10.1371/journal.ppat.1006399

    Figure Lengend Snippet: garA is required for growth of M . tuberculosis in vitro , survival in macrophages, and virulence in mice. ( A ) M . tuberculosis lacking garA was unable to grow on standard 7H10 medium unless supplemented with asparagine. Plasmid-borne garA restored the defect, but variants of garA with mutations at threonine 21 in the phosphorylation motif (ETTS) gave only partial complementation. Strains were grown in Middlebrook 7H9 plus 30 mM asparagine then washed and diluted in standard 7H9 and spotted onto standard 7H10 with or without 10 mM asparagine. Photographs are representative of at least 3 independent experiments. ( B ) M . tuberculosis lacking garA (red squares) had a defect in growth and survival in differentiated THP-1 cells compared to parental M . tuberculosis H37Rv (black circles). Re-introduction of GarA (black triangles) or variants of GarA lacking a single phosphorylation site (grey crosses and squares) restored growth but variant GarA lacking both phosphorylation sites (green triangles) did not. Data points show the mean and standard deviation from four replicates and are representative of two independent experiments. ( C ) M . tuberculosis lacking garA was avirulent in mice as it was eliminated from the lungs. BALB/C mice were infected intranasally with 10 5 bacilli and bacterial burden was measured on days 1, 7, 21 and 28. Data points show the bacterial burden in individual animals. The bacterial burden of mice infected with Δ garA Mt (red squares) was significantly lower than those infected with M . tuberculosis H37Rv (black circles), or complemented Δ garA Mt (black triangles) at all time points from day 7 (p

    Article Snippet: Bacterial strains, media, and culture M . tuberculosis H37Rv and M . smegmatis mc2 155 were routinely cultured on Middlebrook 7H10 agar (Oxoid) with 10% ADN (0.5% bovine serum albumin, 0.2% dextrose, 0.085% NaCl) and Middlebrook 7H9 medium (Oxoid) with 10% ADN and 0.05% Tween 80.

    Techniques: In Vitro, Mouse Assay, Plasmid Preparation, Variant Assay, Standard Deviation, Infection

    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Article Snippet: M. smegmatis mc2 155 was grown in liquid cultures using Middlebrook 7H9 medium (Difco) supplemented with 0.2% glycerol and 0.05% Tween 80 or minimal Hartmans-de Bont (HB) medium ( ) at 37°C.

    Techniques:

    Cell surface of Msmeg-PE11 is highly hydrophobic. ( a ) Pellicle formation of Msmeg-pVV or Msmeg-PE11 was monitored by growing standing cultures of the strains without shaking in Middlebrook 7H9 medium in absence of Tween 80 at 37 °C for various time points. ( b ) Biofilm formation was quantified by crystal violet staining for which Msmeg-pVV or Msmeg-PE11 cells were washed, stained with 1% crystal violet and ethanol extract was spectrophotometrically measured at 570 nm. ( c ) Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 medium with (upper panel) or without (lower panel) 0.05% Tween-80 at 37 °C with shaking for 24 h and 48 h. Cultures were then allowed to settle at room temperature for 30 min. ( d ) In another experiment, Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 broth with congo red (100 μg/ml) and 0.05% Tween 80 for 24 h, 48 h and 72 h at 37 °C. Cells were next washed and re-suspended in acetone. Congo red in the supernatant was spectrophotometrically measured at 490 nm. Data are representative of mean ± SD of three different experiments.

    Journal: Scientific Reports

    Article Title: PE11, a PE/PPE family protein of Mycobacterium tuberculosis is involved in cell wall remodeling and virulence

    doi: 10.1038/srep21624

    Figure Lengend Snippet: Cell surface of Msmeg-PE11 is highly hydrophobic. ( a ) Pellicle formation of Msmeg-pVV or Msmeg-PE11 was monitored by growing standing cultures of the strains without shaking in Middlebrook 7H9 medium in absence of Tween 80 at 37 °C for various time points. ( b ) Biofilm formation was quantified by crystal violet staining for which Msmeg-pVV or Msmeg-PE11 cells were washed, stained with 1% crystal violet and ethanol extract was spectrophotometrically measured at 570 nm. ( c ) Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 medium with (upper panel) or without (lower panel) 0.05% Tween-80 at 37 °C with shaking for 24 h and 48 h. Cultures were then allowed to settle at room temperature for 30 min. ( d ) In another experiment, Msmeg-pVV or Msmeg-PE11 were cultured in 7H9 broth with congo red (100 μg/ml) and 0.05% Tween 80 for 24 h, 48 h and 72 h at 37 °C. Cells were next washed and re-suspended in acetone. Congo red in the supernatant was spectrophotometrically measured at 490 nm. Data are representative of mean ± SD of three different experiments.

    Article Snippet: M. smegmatis culture and transformation M. smegmatis mc2 155 bacteria were grown in Middlebrook 7H9 medium (BD Difco, USA) supplemented with 10% OADC (HiMedia, India), 0.5% glycerol, and 0.05% Tween 80 (Fisher Scientific, USA).

    Techniques: Staining, Cell Culture

    FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in Middlebrook 7H9 medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Mycobacterium avium subsp. paratuberculosis (Map) Fatty Acids Profile Is Strain-Dependent and Changes Upon Host Macrophages Infection

    doi: 10.3389/fcimb.2017.00089

    Figure Lengend Snippet: FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in Middlebrook 7H9 medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.

    Article Snippet: The relative FAs composition of the two isolates recovered from infected BOMAC and MOCL-4 cells was determined by gas chromatography and compared with that of extracellular bacteria and that of bacteria grown in Middlebrook 7H9 medium.

    Techniques: Infection

    Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in 7H9 complete medium

    Journal: Applied and Environmental Microbiology

    Article Title: Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga

    doi: 10.1128/AEM.03765-12

    Figure Lengend Snippet: Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in 7H9 complete medium

    Article Snippet: Mycobacteria were cultured in Middlebrook 7H9 liquid medium (Sigma-Aldrich, Lyon, France) and subcultured at 37°C on Middlebrook and Cohn 7H10 agar (Becton, Dickinson, Le Pont de Claix, France) for 3 days.

    Techniques:

    Mycobacterial size in 7H9 medium. The sizes of M. gilvum (A), M. senegalense (B), M. conceptionense (C), M. rhodesiae (D), M. thermoresistibile (E), M. chelonae (F), M. smegmatis (G), M. abscessus (H), and M. fortuitum subsp. fortuitum (I) were determined

    Journal: Applied and Environmental Microbiology

    Article Title: Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga

    doi: 10.1128/AEM.03765-12

    Figure Lengend Snippet: Mycobacterial size in 7H9 medium. The sizes of M. gilvum (A), M. senegalense (B), M. conceptionense (C), M. rhodesiae (D), M. thermoresistibile (E), M. chelonae (F), M. smegmatis (G), M. abscessus (H), and M. fortuitum subsp. fortuitum (I) were determined

    Article Snippet: Mycobacteria were cultured in Middlebrook 7H9 liquid medium (Sigma-Aldrich, Lyon, France) and subcultured at 37°C on Middlebrook and Cohn 7H10 agar (Becton, Dickinson, Le Pont de Claix, France) for 3 days.

    Techniques:

    51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of Middlebrook 7H9 broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .

    Journal: Frontiers in Chemistry

    Article Title: Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

    doi: 10.3389/fchem.2018.00519

    Figure Lengend Snippet: 51 V NMR (78.9 MHz) spectra are shown of solution of decavanadate (10 mM V 10 , 100 mM V-atoms). The samples are from the bottom up diluted V 10 stock solution (100 mM V-atom) at pH 3.1; 10 mM V 10 in the presence of 0.48 mM and 0.97 mM citrate at pH 2.8 and 2.2, respectively; 10 mM V 10 in the presence of 24 mM P i at pH 6.9; and finally 10 mM V 10 in the presence of Middlebrook 7H9 broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 .

    Article Snippet: The bacteria were grown in 7H9 Middlebrook medium with the addition of D-pantothenate (24 mg/L) at 37°C to an optical density at 600 nm (OD600nm ) of 0.6–0.8.

    Techniques: Nuclear Magnetic Resonance

    51 V NMR (78.9 MHz) spectra are shown of solution of colorless oxovanadate (40 mM V 1 , 40 mM V-atoms). The samples are from the bottom up diluted V 1 stock solution (40 mM V-atom) at pH 8.3; 10 mM V 10 in the presence of 24 mM P i at pH 8.1; 10 mM V 10 in the presence of 0.48 mM citrate at pH 6.3, and finally 10 mM V 10 in the presence of Middlebrook 7H9 broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 . The spectrum labeled Reference is of the capillary reference alone (top spectrum). The key to the signals: V-oligomers, V 1 monomer; V 2 , dimer; V 4 , tetramer; V 5 , pentamer; VCit, V-citrate complex; PV, vanadate-phosphate complex.

    Journal: Frontiers in Chemistry

    Article Title: Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

    doi: 10.3389/fchem.2018.00519

    Figure Lengend Snippet: 51 V NMR (78.9 MHz) spectra are shown of solution of colorless oxovanadate (40 mM V 1 , 40 mM V-atoms). The samples are from the bottom up diluted V 1 stock solution (40 mM V-atom) at pH 8.3; 10 mM V 10 in the presence of 24 mM P i at pH 8.1; 10 mM V 10 in the presence of 0.48 mM citrate at pH 6.3, and finally 10 mM V 10 in the presence of Middlebrook 7H9 broth medium supplemented with 10% ADC enrichment (5% BSA, 2% dextrose, 5% catalase), glycerol (0.2%, v/v) and Tween 80 (0.05%, v/v) recorded both in the absence and the presence of a capillary reference of 100 mM Na 3 VO 4 . The spectrum labeled Reference is of the capillary reference alone (top spectrum). The key to the signals: V-oligomers, V 1 monomer; V 2 , dimer; V 4 , tetramer; V 5 , pentamer; VCit, V-citrate complex; PV, vanadate-phosphate complex.

    Article Snippet: The bacteria were grown in 7H9 Middlebrook medium with the addition of D-pantothenate (24 mg/L) at 37°C to an optical density at 600 nm (OD600nm ) of 0.6–0.8.

    Techniques: Nuclear Magnetic Resonance, Labeling

    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Article Snippet: Moreover, when M. gordonae was grown in filtered water supplemented with 10% 7H9 Middlebrook liquid medium, the inactivation rates decreased by a factor 10.

    Techniques: Concentration Assay

    Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Article Snippet: Moreover, when M. gordonae was grown in filtered water supplemented with 10% 7H9 Middlebrook liquid medium, the inactivation rates decreased by a factor 10.

    Techniques: Concentration Assay

    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Journal: Frontiers in Microbiology

    Article Title: Functional Studies of Five Toxin-Antitoxin Modules in Mycobacterium tuberculosis H37Rv

    doi: 10.3389/fmicb.2016.02071

    Figure Lengend Snippet: Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Article Snippet: M. smegmatis mc2 155 was grown in Middlebrook 7H9 (a liquid medium, Difco) containing 0.2% glycerol and 0.02% Tween 80 or Middlebrook 7H10 (a solid agar medium, Difco) containing 0.2% glycerol.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation

    M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in Middlebrook 7H9 medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.

    Journal: eLife

    Article Title: Cdc48-like protein of actinobacteria (Cpa) is a novel proteasome interactor in mycobacteria and related organisms

    doi: 10.7554/eLife.34055

    Figure Lengend Snippet: M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in Middlebrook 7H9 medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.

    Article Snippet: Blue clones were used to inoculate fresh Middlebrook 7H9 medium containing kanamycin and hygromycin and incubated for several days at 37°C.

    Techniques: Knock-Out, Cell Culture

    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Article Snippet: In this study, conditions similar to those used by Taylor et al. ( ) were chosen: tests were performed at pH 7 and 23°C with strains grown in 7H9 Middlebrook broth medium.

    Techniques: Concentration Assay

    Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Article Snippet: In this study, conditions similar to those used by Taylor et al. ( ) were chosen: tests were performed at pH 7 and 23°C with strains grown in 7H9 Middlebrook broth medium.

    Techniques: Concentration Assay

    The growth curves for M. paratuberculosis JTC114 were obtained from cultures grown in 7H9-OADC (A) or WR-GD (B) medium at pHs 6.0 and 6.8. The photographs present surface morphologies in liquid culture flasks of M. paratuberculosis JTC114 at the stationary phase in 7H9-OADC (C) or WR-GD (D) medium at pH 6.0.

    Journal: Applied and Environmental Microbiology

    Article Title: Variation in Resistance of Mycobacterium paratuberculosis to Acid Environments as a Function of Culture Medium

    doi: 10.1128/AEM.69.11.6833-6840.2003

    Figure Lengend Snippet: The growth curves for M. paratuberculosis JTC114 were obtained from cultures grown in 7H9-OADC (A) or WR-GD (B) medium at pHs 6.0 and 6.8. The photographs present surface morphologies in liquid culture flasks of M. paratuberculosis JTC114 at the stationary phase in 7H9-OADC (C) or WR-GD (D) medium at pH 6.0.

    Article Snippet: The study of Spahr and Schafroth investigating M. paratuberculosis survival in cheese used Middlebrook 7H9-OADC medium supplemented with 0.5% (vol/vol) glycerol ( ).

    Techniques:

    (Upper panel) SDS-PAGE soluble protein profiles for M. paratuberculosis strain JTC303 grown at pH 6.0 or 6.8 in 7H9-OADC, WR-GD, or 7H9-GD medium. The amount of total soluble proteins applied per lane was 30 μg. STD, standard. (Lower panel) Relative intensity levels of the protein bands for the strain grown in glycerol-containing media (WR-GD and 7H9-GD) versus 7H9-OADC medium.

    Journal: Applied and Environmental Microbiology

    Article Title: Variation in Resistance of Mycobacterium paratuberculosis to Acid Environments as a Function of Culture Medium

    doi: 10.1128/AEM.69.11.6833-6840.2003

    Figure Lengend Snippet: (Upper panel) SDS-PAGE soluble protein profiles for M. paratuberculosis strain JTC303 grown at pH 6.0 or 6.8 in 7H9-OADC, WR-GD, or 7H9-GD medium. The amount of total soluble proteins applied per lane was 30 μg. STD, standard. (Lower panel) Relative intensity levels of the protein bands for the strain grown in glycerol-containing media (WR-GD and 7H9-GD) versus 7H9-OADC medium.

    Article Snippet: The study of Spahr and Schafroth investigating M. paratuberculosis survival in cheese used Middlebrook 7H9-OADC medium supplemented with 0.5% (vol/vol) glycerol ( ).

    Techniques: SDS Page

    M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in ADC-supplemented Middlebrook 7H9 medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P

    Journal: Frontiers in Microbiology

    Article Title: Interfering With DNA Decondensation as a Strategy Against Mycobacteria

    doi: 10.3389/fmicb.2018.02034

    Figure Lengend Snippet: M. tuberculosis condenses DNA during starvation. M. tuberculosis mc 2 6030 was cultured in ADC-supplemented Middlebrook 7H9 medium before being starved in PBS. Lipid distribution and DNA localization was imaged using Nile Red (red) and Hoechst (green) respectively at day 0, 6, and 10 (A) and percentage bacteria with condensed DNA was quantified at day 0, 3, 6, and 10. Values represent mean percentage bacteria with condense DNA ± standard error, pooled data of 2 measurements, * P

    Article Snippet: M. smegmatis was cultured in ADC-supplemented Middlebrook 7H9 medium before being transferred to PBS.

    Techniques: Cell Culture