middlebrook 7h11 agar Search Results


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  • 99
    Thermo Fisher middlebrook 7h11 agar
    Middlebrook 7h11 Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middlebrook 7h11 agar
    Middlebrook 7h11 Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11 agar
    Anti-tubercular activity of thiosulfinate-derivative rich extract of garlic in comparison to standard drugs in 106 cells/well of RAW 264.7 macrophage cells infected with Mycobacterium tuberculosis H37Rv (5 × 106 colony forming unit/well). The results are expressed as mean ± standard deviation of three sets of experiments. After 1 and 4 days of incubation, the cells were lysed using 0.06% sodium dodecyl sulfate and plated on <t>Middlebrook</t> <t>7H11</t> agar plates after dilution in 1:10 ratio. (a) Number of colony forming units after 1 and 4 days treatment with extract and standard drugs. (b) log 10 (reduction in colony forming units) after 1 and 4 days treatment with standard drugs
    Middlebrook 7h11 Agar, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 464 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson mb7h11 agar
    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective <t>MB7H11</t> agar after 6 days of preexposure culturing
    Mb7h11 Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific middlebrook 7h11 agar
    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective <t>MB7H11</t> agar after 6 days of preexposure culturing
    Middlebrook 7h11 Agar, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hardy Diagnostics middlebrook 7h11 agar
    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective <t>MB7H11</t> agar after 6 days of preexposure culturing
    Middlebrook 7h11 Agar, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7h11 7h11 selective agar biplate
    Erdman bacterial load in lung, liver, and spleen tissues. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. Up to 19 weeks post infection, whole lung collected and homogenized in 1×PBS, serially diluted, and plated onto <t>7H11</t> agar plates. Data are represented as Log10 CFU/organ. Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. * P
    7h11 7h11 Selective Agar Biplate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 agar bd
    Erdman bacterial load in lung, liver, and spleen tissues. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. Up to 19 weeks post infection, whole lung collected and homogenized in 1×PBS, serially diluted, and plated onto <t>7H11</t> agar plates. Data are represented as Log10 CFU/organ. Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. * P
    Middlebrook 7h11 Agar Bd, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h11 agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Agar, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 2331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Appleton Woods Ltd middlebrook 7h11 agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Agar, supplied by Appleton Woods Ltd, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BVA Scientific middlebrook 7h11 agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Agar, supplied by BVA Scientific, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 oadc agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Oadc Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Molecular Toxicology Inc middlebrook 7h11 agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Agar, supplied by Molecular Toxicology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories middlebrook 7h11 agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Agar, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bioMerieux middlebrook 7h11 agar
    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on <t>Middlebrook</t> <t>7H11</t> agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.
    Middlebrook 7h11 Agar, supplied by bioMerieux, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h11 agar plates
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
    Middlebrook 7h11 Agar Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals difco middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
    Difco Middlebrook 7h11 Agar, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals selective middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
    Selective Middlebrook 7h11 Agar, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals prepared middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals traditional middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals sterile middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals nutrient middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals oadc supplemented middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
    Oadc Supplemented Middlebrook 7h11 Agar, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals oadc enriched middlebrook 7h11 agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals 7h11 middlebrook 7h11 selective agar biplate
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    Difco middlebrook 7h11 oadc agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals middlebrook 7h11 mitchison agar
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
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    MiddleBrook Pharmaceuticals pbs middlebrook 7h11 agar plates
    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto <t>7H11</t> <t>Middlebrook</t> agar plates, and cfu were quantified. Data are for n = 4 experiments; p
    Pbs Middlebrook 7h11 Agar Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Anti-tubercular activity of thiosulfinate-derivative rich extract of garlic in comparison to standard drugs in 106 cells/well of RAW 264.7 macrophage cells infected with Mycobacterium tuberculosis H37Rv (5 × 106 colony forming unit/well). The results are expressed as mean ± standard deviation of three sets of experiments. After 1 and 4 days of incubation, the cells were lysed using 0.06% sodium dodecyl sulfate and plated on Middlebrook 7H11 agar plates after dilution in 1:10 ratio. (a) Number of colony forming units after 1 and 4 days treatment with extract and standard drugs. (b) log 10 (reduction in colony forming units) after 1 and 4 days treatment with standard drugs

    Journal: Pharmacognosy Magazine

    Article Title: Allium sativum Constituents Exhibit Anti-tubercular Activity In vitro and in RAW 264.7 Mouse Macrophage Cells Infected with Mycobacterium tuberculosis H37Rv

    doi: 10.4103/pm.pm_435_16

    Figure Lengend Snippet: Anti-tubercular activity of thiosulfinate-derivative rich extract of garlic in comparison to standard drugs in 106 cells/well of RAW 264.7 macrophage cells infected with Mycobacterium tuberculosis H37Rv (5 × 106 colony forming unit/well). The results are expressed as mean ± standard deviation of three sets of experiments. After 1 and 4 days of incubation, the cells were lysed using 0.06% sodium dodecyl sulfate and plated on Middlebrook 7H11 agar plates after dilution in 1:10 ratio. (a) Number of colony forming units after 1 and 4 days treatment with extract and standard drugs. (b) log 10 (reduction in colony forming units) after 1 and 4 days treatment with standard drugs

    Article Snippet: The lysates were diluted in 1:10 ratio with sterile PBS and 10 μL was plated on Middlebrook 7H11 agar (Difco) supplemented with OADC.

    Techniques: Activity Assay, Infection, Standard Deviation, Incubation

    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective MB7H11 agar after 6 days of preexposure culturing

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Pyrazinamide Is Active against Mycobacterium tuberculosis Cultures at Neutral pH and Low Temperature

    doi: 10.1128/AAC.00654-16

    Figure Lengend Snippet: Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective MB7H11 agar after 6 days of preexposure culturing

    Article Snippet: In short, aliquots of liquid cultures, sieved through a 5-μm-pore filter, were inoculated on 8 by 8-mm squares of porous supports on nonselective MB7H11 agar (BD, Sparks, MD, USA) supplemented with 0.5% glycerol and 10% oleic acid-albumin-dextrose-catalase (OADC) (BD).

    Techniques:

    Erdman bacterial load in lung, liver, and spleen tissues. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. Up to 19 weeks post infection, whole lung collected and homogenized in 1×PBS, serially diluted, and plated onto 7H11 agar plates. Data are represented as Log10 CFU/organ. Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. * P

    Journal: International journal of immunopathology and pharmacology

    Article Title: CHO expressed recombinant human lactoferrin as an adjuvant for BCG

    doi: 10.1177/0394632015599832

    Figure Lengend Snippet: Erdman bacterial load in lung, liver, and spleen tissues. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. Up to 19 weeks post infection, whole lung collected and homogenized in 1×PBS, serially diluted, and plated onto 7H11 agar plates. Data are represented as Log10 CFU/organ. Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. * P

    Article Snippet: The homogenized tissue was serially diluted in 1×PBS, plated (100 μL/plate) onto Middlebrook 7H11 agar plates (Remel), and incubated at 37°C.

    Techniques: Mouse Assay, Infection

    MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on Middlebrook 7H11 agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Correlation of different phenotypic drug susceptibility testing methods for four fluoroquinolones in Mycobacterium tuberculosis

    doi: 10.1093/jac/dkv499

    Figure Lengend Snippet: MIC distribution of ofloxacin on Löwenstein–Jensen medium stratified to the historical resistance profile on Middlebrook 7H11 agar as well as gyrAB mutations. OFX, ofloxacin; S, susceptible; R, resistant; LJ, Löwenstein–Jensen.

    Article Snippet: For the proportion method on 7H11, ofloxacin susceptibility testing was done on Middlebrook 7H11 agar with 2 mg/L critical concentration, as per routine testing (Table ).

    Techniques:

    (Dis)agreement between Löwenstein–Jensen medium, Middlebrook 7H11 agar, REMA and MGIT 960 regarding ofloxacin susceptibility (cut-off 2 mg/L) considering Löwenstein–Jensen as gold standard: (a) all 91 M. tuberculosis strains and (b) excluding strains with an MIC around the cut-off (2 or 4 mg/L). Numbers inside the Venn diagram indicate ofloxacin-resistant strains in one or more methods. Black, concordantly resistant in all methods; light grey, ofloxacin resistant on Löwenstein–Jensen and resistant by one or more other methods; and dark grey, ofloxacin resistant by one or more methods, yet susceptible on Löwenstein–Jensen. W, gyrAB WT; M, gyrAB mutant; OFX, ofloxacin.

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Correlation of different phenotypic drug susceptibility testing methods for four fluoroquinolones in Mycobacterium tuberculosis

    doi: 10.1093/jac/dkv499

    Figure Lengend Snippet: (Dis)agreement between Löwenstein–Jensen medium, Middlebrook 7H11 agar, REMA and MGIT 960 regarding ofloxacin susceptibility (cut-off 2 mg/L) considering Löwenstein–Jensen as gold standard: (a) all 91 M. tuberculosis strains and (b) excluding strains with an MIC around the cut-off (2 or 4 mg/L). Numbers inside the Venn diagram indicate ofloxacin-resistant strains in one or more methods. Black, concordantly resistant in all methods; light grey, ofloxacin resistant on Löwenstein–Jensen and resistant by one or more other methods; and dark grey, ofloxacin resistant by one or more methods, yet susceptible on Löwenstein–Jensen. W, gyrAB WT; M, gyrAB mutant; OFX, ofloxacin.

    Article Snippet: For the proportion method on 7H11, ofloxacin susceptibility testing was done on Middlebrook 7H11 agar with 2 mg/L critical concentration, as per routine testing (Table ).

    Techniques: Mutagenesis

    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto 7H11 Middlebrook agar plates, and cfu were quantified. Data are for n = 4 experiments; p

    Journal: The Journal of Biological Chemistry

    Article Title: Keratinocyte Growth Factor Administration Attenuates Murine Pulmonary Mycobacterium tuberculosis Infection through Granulocyte-Macrophage Colony-stimulating Factor (GM-CSF)-dependent Macrophage Activation and Phagolysosome Fusion *

    doi: 10.1074/jbc.M114.591891

    Figure Lengend Snippet: KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto 7H11 Middlebrook agar plates, and cfu were quantified. Data are for n = 4 experiments; p

    Article Snippet: After 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto 7H11 Middlebrook agar plates, and M. tuberculosis cfu were quantified.

    Techniques: Cell Culture, Infection, Co-Culture Assay

    Bactericidal activity of compounds 1 and 5 for nonreplicating M. tuberculosis . Nonreplicating wild-type M. tuberculosis at an OD 580 of 0.01 was exposed to compounds for 7 days, and surviving bacilli were enumerated on 7H11-OADC agar plates. The inoculum is shown in yellow. The limit of detection was 1 colony arising from 10 μL of undiluted sample. Error bars represent standard deviations of triplicates. One of two similar experiments.

    Journal: Journal of Medicinal Chemistry

    Article Title: Novel Cephalosporins Selectively Active on Nonreplicating Mycobacterium tuberculosis

    doi: 10.1021/acs.jmedchem.5b01833

    Figure Lengend Snippet: Bactericidal activity of compounds 1 and 5 for nonreplicating M. tuberculosis . Nonreplicating wild-type M. tuberculosis at an OD 580 of 0.01 was exposed to compounds for 7 days, and surviving bacilli were enumerated on 7H11-OADC agar plates. The inoculum is shown in yellow. The limit of detection was 1 colony arising from 10 μL of undiluted sample. Error bars represent standard deviations of triplicates. One of two similar experiments.

    Article Snippet: At select time points, aliquots of cells were serially diluted in PBS-Tyl and spread on Middlebrook 7H11 agar plates containing a 10% OADC supplement.

    Techniques: Activity Assay

    DPI demonstrated a dose dependent bactericidal activity against M. tuberculosis and S. aureus . Killing curve for various drugs depicting concentration dependent killing in M. tuberculosis ( A ) replicative media, ( B ) non-replicative media, ( C ) in S. aureus, bacterial enumeration was done by CFU plating at different time points on 7H11 + OADC plates and MHA plates for M.tb and S. aureus respectively.

    Journal: Scientific Reports

    Article Title: Diphenyleneiodonium chloride (DPIC) displays broad-spectrum bactericidal activity

    doi: 10.1038/s41598-017-11575-5

    Figure Lengend Snippet: DPI demonstrated a dose dependent bactericidal activity against M. tuberculosis and S. aureus . Killing curve for various drugs depicting concentration dependent killing in M. tuberculosis ( A ) replicative media, ( B ) non-replicative media, ( C ) in S. aureus, bacterial enumeration was done by CFU plating at different time points on 7H11 + OADC plates and MHA plates for M.tb and S. aureus respectively.

    Article Snippet: For evaluating the reduction in CFU, 0.1 mL sample was removed at various time-points, serially 10-fold diluted in 0.9 mL phosphate buffer saline and 0.1 mL of the respective dilution was spread on Middlebrook 7H11 agar plate supplemented with OADC.

    Techniques: Activity Assay, Concentration Assay

    DPIC inhibits the intracellular replication of M. tuberculosis inside mouse Bone marrow-derived macrophages. Survival of M. tuberculosis in BMDM in the presence of INH and DPIC, drug treatment was done four hours post infection, and were replenished on day 4. Bacterial enumeration was done by lysing the cells with 0.01% triton X-100 and CFU plating on 7H11 + OADC plates.

    Journal: Scientific Reports

    Article Title: Diphenyleneiodonium chloride (DPIC) displays broad-spectrum bactericidal activity

    doi: 10.1038/s41598-017-11575-5

    Figure Lengend Snippet: DPIC inhibits the intracellular replication of M. tuberculosis inside mouse Bone marrow-derived macrophages. Survival of M. tuberculosis in BMDM in the presence of INH and DPIC, drug treatment was done four hours post infection, and were replenished on day 4. Bacterial enumeration was done by lysing the cells with 0.01% triton X-100 and CFU plating on 7H11 + OADC plates.

    Article Snippet: For evaluating the reduction in CFU, 0.1 mL sample was removed at various time-points, serially 10-fold diluted in 0.9 mL phosphate buffer saline and 0.1 mL of the respective dilution was spread on Middlebrook 7H11 agar plate supplemented with OADC.

    Techniques: Derivative Assay, Infection

    KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto 7H11 Middlebrook agar plates, and cfu were quantified. Data are for n = 4 experiments; p

    Journal: The Journal of Biological Chemistry

    Article Title: Keratinocyte Growth Factor Administration Attenuates Murine Pulmonary Mycobacterium tuberculosis Infection through Granulocyte-Macrophage Colony-stimulating Factor (GM-CSF)-dependent Macrophage Activation and Phagolysosome Fusion *

    doi: 10.1074/jbc.M114.591891

    Figure Lengend Snippet: KGF treatment of MLE-15 cells results in a decrease in M. tuberculosis burden in co-cultured RAW cells and induces GM-CSF production. To determine the mechanism of KGF-enhanced M. tuberculosis clearance, MLE-15 cells plated in the lower well of transwell plates were pretreated with KGF (100 ng/ml) or medium only overnight. RAW cells infected with M. tuberculosis were then added to the upper well. A , after 5 days of co-culture, the RAW cells were harvested, lysed, and plated onto 7H11 Middlebrook agar plates, and cfu were quantified. Data are for n = 4 experiments; p

    Article Snippet: Viable M. tuberculosis in the lung tissue homogenates was quantified by serial dilution, plating in duplicate onto 7H11 Middlebrook agar in 6-well plates, incubation at 37 °C in a 5% CO2 incubator for 3 weeks, and counting of cfu ( ).

    Techniques: Cell Culture, Infection, Co-Culture Assay

    Ex vivo susceptibility of multi-drug resistant strains of M.tb to trifluoperazine. THP-1 cells and MDMs infected with M.tb at an MOI of 10∶1were treated with different concentrations (0, 1, 2.5 and 5 µg/ml) in duplicates as described in materials and methods . Post 1, 2 and 3 days of infection, cells were lysed and plated on 7H11 to enumerate CFUs. Graphs depict intracellular growth perturbation of ( a ) M.tb H37Rv in THP-1 cells, ( b ) M.tb JAL2287in THP-1 cells, ( c ) M.tb 1934 in THP-1 cells, ( d ) M.tb H37Rv in MDMs and ( e ) M.tb JAL2287 in MDMs in presence of various concentrations of TFP. Values represent means ± standard errors of the means of duplicates.

    Journal: PLoS ONE

    Article Title: Activity of Trifluoperazine against Replicating, Non-Replicating and Drug Resistant M. tuberculosis

    doi: 10.1371/journal.pone.0044245

    Figure Lengend Snippet: Ex vivo susceptibility of multi-drug resistant strains of M.tb to trifluoperazine. THP-1 cells and MDMs infected with M.tb at an MOI of 10∶1were treated with different concentrations (0, 1, 2.5 and 5 µg/ml) in duplicates as described in materials and methods . Post 1, 2 and 3 days of infection, cells were lysed and plated on 7H11 to enumerate CFUs. Graphs depict intracellular growth perturbation of ( a ) M.tb H37Rv in THP-1 cells, ( b ) M.tb JAL2287in THP-1 cells, ( c ) M.tb 1934 in THP-1 cells, ( d ) M.tb H37Rv in MDMs and ( e ) M.tb JAL2287 in MDMs in presence of various concentrations of TFP. Values represent means ± standard errors of the means of duplicates.

    Article Snippet: Ten-fold serial dilutions were prepared with 7H9 broth and 50 µl of each dilution was plated on 7H11 agar supplemented with Middlebrook OADC enrichment.

    Techniques: Ex Vivo, Infection

    Effect of crude RPF and recombinant RPF proteins on Mtb growth. Sputum samples from 33 smear-negative/culture-negative TB patients were treated with placebo (H9) or crude RPF or recombinant RPF protein (rRPF). Treated samples were plated on 7H11 agar to enumerate bacterial CFU. The difference in bacterial CFU was statistically significant between H9 and RPF ( P = 0.03), H9 and rRPF ( P = 0.049), and RPF and rRPF ( P = 0.032) as analyzed by one-way ANOVA with the post hoc test.

    Journal: Frontiers in Microbiology

    Article Title: Differential Culturability of Mycobacterium tuberculosis in Culture-Negative Sputum of Patients With Pulmonary Tuberculosis and in a Simulated Model of Dormancy

    doi: 10.3389/fmicb.2019.02381

    Figure Lengend Snippet: Effect of crude RPF and recombinant RPF proteins on Mtb growth. Sputum samples from 33 smear-negative/culture-negative TB patients were treated with placebo (H9) or crude RPF or recombinant RPF protein (rRPF). Treated samples were plated on 7H11 agar to enumerate bacterial CFU. The difference in bacterial CFU was statistically significant between H9 and RPF ( P = 0.03), H9 and rRPF ( P = 0.049), and RPF and rRPF ( P = 0.032) as analyzed by one-way ANOVA with the post hoc test.

    Article Snippet: Bacterial Strains Strains of M. tuberculosis (H37Rv, H37Ra, Erdman, DRBL2, MTB01, MTB02), Mycobacterium smegmatis , and Escherichia coli DH5α were grown and maintained as per standard procedures in LJ slants, Middlebrook 7H9 broth, and Middlebrook 7H11 agar media supplemented with oleic acid dextrose and catalase enrichment (OADC) (BD Biosciences) and glycerol or in Luria-Bertani (LB) media (for E. coli ) .

    Techniques: Recombinant

    Effect of crude RPF isolated from different bacterial strains in resuscitating dormant clinical Mtb isolates. Clinical Mtb isolates MTB01 (A) and MTB02 (B) were grown to dormancy in an in vitro hypoxia model and treated with crude RPF isolated from E. coli (DH5a), M. smegmatis mc 2 155, Mtb H37Ra, Mtb H37Ra, Mtb 1338-EAI, Mtb DRBL2-BEI, and Mtb Erdmann strains or placebo (7H9 broth; C1). Treated Mtb culture was plated on 7H11 agar media to enumerate bacterial CFU. The data in A and B , except C1 and DH5, are significant; P

    Journal: Frontiers in Microbiology

    Article Title: Differential Culturability of Mycobacterium tuberculosis in Culture-Negative Sputum of Patients With Pulmonary Tuberculosis and in a Simulated Model of Dormancy

    doi: 10.3389/fmicb.2019.02381

    Figure Lengend Snippet: Effect of crude RPF isolated from different bacterial strains in resuscitating dormant clinical Mtb isolates. Clinical Mtb isolates MTB01 (A) and MTB02 (B) were grown to dormancy in an in vitro hypoxia model and treated with crude RPF isolated from E. coli (DH5a), M. smegmatis mc 2 155, Mtb H37Ra, Mtb H37Ra, Mtb 1338-EAI, Mtb DRBL2-BEI, and Mtb Erdmann strains or placebo (7H9 broth; C1). Treated Mtb culture was plated on 7H11 agar media to enumerate bacterial CFU. The data in A and B , except C1 and DH5, are significant; P

    Article Snippet: Bacterial Strains Strains of M. tuberculosis (H37Rv, H37Ra, Erdman, DRBL2, MTB01, MTB02), Mycobacterium smegmatis , and Escherichia coli DH5α were grown and maintained as per standard procedures in LJ slants, Middlebrook 7H9 broth, and Middlebrook 7H11 agar media supplemented with oleic acid dextrose and catalase enrichment (OADC) (BD Biosciences) and glycerol or in Luria-Bertani (LB) media (for E. coli ) .

    Techniques: Isolation, In Vitro

    Disturbance in gut microbiota by antibiotics increased the survival of Mtb in the lungs and its dissemination to other organs . Mice treated with antibiotics prior and post exposure to Mtb infection. Later, animals were sacrificed, and lungs were isolated. Bacterial burden in (A) pre-antibiotics and (B) post-antibiotics-treated group was estimated by plating serial dilutions of lung homogenate on 7H11 agar plates. Colonies were enumerated on 21 days of plating. Bar graph depicts the bacterial burden in lungs. Data shown as mean ± SEM are representative of three independent experiments ( n = 4–5 mice/group). (C) Histopathology sections of lungs of pre-antibiotics and post-antibiotics group were H E stained and imaged at a magnification 40×. (D) Bar graphs depict the percentage of tuberculous region of pre-antibiotics Mtb group. Data are representative of three independent experiments ( n = 4–5 animals/group) (* p

    Journal: Frontiers in Immunology

    Article Title: Alteration in the Gut Microbiota Provokes Susceptibility to Tuberculosis

    doi: 10.3389/fimmu.2016.00529

    Figure Lengend Snippet: Disturbance in gut microbiota by antibiotics increased the survival of Mtb in the lungs and its dissemination to other organs . Mice treated with antibiotics prior and post exposure to Mtb infection. Later, animals were sacrificed, and lungs were isolated. Bacterial burden in (A) pre-antibiotics and (B) post-antibiotics-treated group was estimated by plating serial dilutions of lung homogenate on 7H11 agar plates. Colonies were enumerated on 21 days of plating. Bar graph depicts the bacterial burden in lungs. Data shown as mean ± SEM are representative of three independent experiments ( n = 4–5 mice/group). (C) Histopathology sections of lungs of pre-antibiotics and post-antibiotics group were H E stained and imaged at a magnification 40×. (D) Bar graphs depict the percentage of tuberculous region of pre-antibiotics Mtb group. Data are representative of three independent experiments ( n = 4–5 animals/group) (* p

    Article Snippet: These antibiotics exhibited no impact on Mtb recovery in a Middlebrook 7H11 agar formulation ( ).

    Techniques: Mouse Assay, Infection, Isolation, Histopathology, Staining