middlebrook 7h11 Search Results


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  • 99
    Thermo Fisher middlebrook 7h11
    Middlebrook 7h11, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middlebrook 7h11
    Middlebrook 7h11, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 mb7h11
    Middlebrook 7h11 Mb7h11, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 79/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11
    Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on <t>Middlebrook</t> <t>7H11</t> agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .
    Middlebrook 7h11, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad middlebrook 7h11
    Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on <t>Middlebrook</t> <t>7H11</t> agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .
    Middlebrook 7h11, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hardy Diagnostics middlebrook 7h11
    Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on <t>Middlebrook</t> <t>7H11</t> agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .
    Middlebrook 7h11, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories middlebrook 7h11
    Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on <t>Middlebrook</t> <t>7H11</t> agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .
    Middlebrook 7h11, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11 oadc
    Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on <t>Middlebrook</t> <t>7H11</t> agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .
    Middlebrook 7h11 Oadc, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 plates
    Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on <t>Middlebrook</t> 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook <t>7H11</t> agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P
    Middlebrook 7h11 Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11 plates
    Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on <t>Middlebrook</t> 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook <t>7H11</t> agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P
    Middlebrook 7h11 Plates, supplied by Difco, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 slant
    Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on <t>Middlebrook</t> 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook <t>7H11</t> agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P
    Middlebrook 7h11 Slant, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 biplate
    Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on <t>Middlebrook</t> 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook <t>7H11</t> agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P
    Middlebrook 7h11 Biplate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11 broth
    Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on <t>Middlebrook</t> 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook <t>7H11</t> agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P
    Middlebrook 7h11 Broth, supplied by Difco, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson mb7h11 agar
    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective <t>MB7H11</t> agar after 6 days of preexposure culturing
    Mb7h11 Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 87/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h11 oadc
    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective <t>MB7H11</t> agar after 6 days of preexposure culturing
    Middlebrook 7h11 Oadc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11 agar
    Anti-tubercular activity of thiosulfinate-derivative rich extract of garlic in comparison to standard drugs in 106 cells/well of RAW 264.7 macrophage cells infected with Mycobacterium tuberculosis H37Rv (5 × 106 colony forming unit/well). The results are expressed as mean ± standard deviation of three sets of experiments. After 1 and 4 days of incubation, the cells were lysed using 0.06% sodium dodecyl sulfate and plated on <t>Middlebrook</t> <t>7H11</t> agar plates after dilution in 1:10 ratio. (a) Number of colony forming units after 1 and 4 days treatment with extract and standard drugs. (b) log 10 (reduction in colony forming units) after 1 and 4 days treatment with standard drugs
    Middlebrook 7h11 Agar, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 464 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h11 medium
    Comparison of the colony morphology of the M. tuberculosis wild type, the M. tuberculosis Δ pykA knockout mutant, the knockout complemented with pLK102 containing the pykA gene, and M. bovis . The strains are arranged from left to right, each grown on <t>Middlebrook</t> <t>7H11</t> plates with glycerol plus OADC (g), OADC only (o), and pyruvate plus OADC (p).
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    Becton Dickinson middlebrook 7h11 medium
    Comparison of the colony morphology of the M. tuberculosis wild type, the M. tuberculosis Δ pykA knockout mutant, the knockout complemented with pLK102 containing the pykA gene, and M. bovis . The strains are arranged from left to right, each grown on <t>Middlebrook</t> <t>7H11</t> plates with glycerol plus OADC (g), OADC only (o), and pyruvate plus OADC (p).
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    Fisher Scientific middlebrook 7h11 agar
    Comparison of the colony morphology of the M. tuberculosis wild type, the M. tuberculosis Δ pykA knockout mutant, the knockout complemented with pLK102 containing the pykA gene, and M. bovis . The strains are arranged from left to right, each grown on <t>Middlebrook</t> <t>7H11</t> plates with glycerol plus OADC (g), OADC only (o), and pyruvate plus OADC (p).
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    MiddleBrook Pharmaceuticals middlebrook 7h11
    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    Hardy Diagnostics middlebrook 7h11 agar
    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    Becton Dickinson middlebrook 7h11 ampicillin plates
    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
    Middlebrook 7h11 Ampicillin Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7h11 7h11 selective agar biplate
    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
    7h11 7h11 Selective Agar Biplate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h11 broth
    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on <t>Middlebrook</t> <t>7H11-enriched</t> media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.
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    Image Search Results


    Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on Middlebrook 7H11 agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .

    Journal: Yonsei Medical Journal

    Article Title: Kinetics of IFN-Gamma and TNF-Alpha Gene Expression and Their Relationship with Disease Progression after Infection with Mycobacterium Tuberculosis in Guinea Pigs

    doi: 10.3349/ymj.2013.54.3.707

    Figure Lengend Snippet: Growth curves of M. tb in each organ. The M. tb growth in lungs, BTLNs, spleens and livers of guinea pigs were measured after an aerosol infection with about 10 6 colony forming units (CFUs) per exposure. The number of mean viable CFUs in each organ at different time intervals following the infection was calculated by plating the serial dilutions of homogenates of each organ on Middlebrook 7H11 agar and counting CFUs after 14-21 days. BTLN, bronchotracheal lymph node; M. tb , Mycobacterium tuberculosis .

    Article Snippet: Homogenates were serially diluted 10-folds and plated onto Middlebrook 7H11 (Difco Laboratories, Detroit, MI, USA) agar plates, which were incubated at 37℃ for 14-21 days.

    Techniques: Infection

    Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on Middlebrook 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook 7H11 agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Adoptive transfer of phosphoantigen-specific γδ T-cell subset attenuates M. tuberculosis infection in nonhuman primates

    doi: 10.4049/jimmunol.1602019

    Figure Lengend Snippet: Vγ2Vδ2 T cells generated for adoptive transfer exhibited multi-effector functions for producing anti-Mtb cytokines and inhibiting intracellular mycobacteria A). Representative flow histograms showing the percentage of Vγ2Vδ2 T cells in total CD3+ T-cell population before and after 12 days of ex vivo expansion using zoledronate and IL-2. B). Representative flow histograms showing ex vivo expanded Vγ2Vδ2 T cells producing IFN-γ, TNF-α, Granzyme A and Granzyme B after 12-day culture with Zoledronate/IL-2. Panels were gated on CD3+ lymphocytes. Numbers in upper-right quadrant indicate the percentages of cytokine-producing Vγ2+ T cells in total Vγ2+ population. C). Graph data showing percentages of cytokines-producing Vγ2Vδ2 T-cell subsets in total Vγ2+ population with or without HMBPP stimulation. D). Representative flow histograms (left) show expression levels of the degranulation marker CD107a by expanded Vγ2Vδ2 T cells in the absence or presence of HMBPP. Graph data (right) demonstrate pool mean percentages of CD107a+Vγ2+ T cells in total Vγ2+ population with or without HMBPP stimulation. E). Ex vivo expanded Vγ2Vδ2 T cells could kill/inhibit intracellular BCG(left) and Mtb(right) bacilli. Expanded Vγ2Vδ2 T cells were co-cultured with BCG-infected ThP1 monocytes at an effector to target ratio of 10:1 in the presence of blocking antibodies against IFN-γ, TNF-α, or Granzyme A or mouse IgG isotype. Cultured cells were lysed and lysates were serially diluted and plated on Middlebrook 7H10 agar plates. For the intracellular Mtb inhibition assay, expanded Vγ2Vδ2 T cells were similarly incubated for 72 hrs with autologous monocytes/macrophages infected with Mtb at a MOI of 10:1. Cells were then lysed, and lysates were plated on Middlebrook 7H11 agar. Data in (A) to (E) are derived from 3–5 independent experiments. Values are expressed as means ± SEM. P values are results from non-parametric t test. * P

    Article Snippet: 5-fold serial dilutions of samples were plated on Middlebrook 7H11 plate (BD).

    Techniques: Generated, Adoptive Transfer Assay, Flow Cytometry, Ex Vivo, Expressing, Marker, Cell Culture, Infection, Blocking Assay, Inhibition, Incubation, Derivative Assay

    Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective MB7H11 agar after 6 days of preexposure culturing

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Pyrazinamide Is Active against Mycobacterium tuberculosis Cultures at Neutral pH and Low Temperature

    doi: 10.1128/AAC.00654-16

    Figure Lengend Snippet: Growth rates (left) and colony size (right) of PZA-susceptible and -resistant M. tuberculosis strains exposed to PZA or POA at 28°C. Strains were inoculated on solid porous supports on nonselective MB7H11 agar after 6 days of preexposure culturing

    Article Snippet: In short, aliquots of liquid cultures, sieved through a 5-μm-pore filter, were inoculated on 8 by 8-mm squares of porous supports on nonselective MB7H11 agar (BD, Sparks, MD, USA) supplemented with 0.5% glycerol and 10% oleic acid-albumin-dextrose-catalase (OADC) (BD).

    Techniques:

    Anti-tubercular activity of thiosulfinate-derivative rich extract of garlic in comparison to standard drugs in 106 cells/well of RAW 264.7 macrophage cells infected with Mycobacterium tuberculosis H37Rv (5 × 106 colony forming unit/well). The results are expressed as mean ± standard deviation of three sets of experiments. After 1 and 4 days of incubation, the cells were lysed using 0.06% sodium dodecyl sulfate and plated on Middlebrook 7H11 agar plates after dilution in 1:10 ratio. (a) Number of colony forming units after 1 and 4 days treatment with extract and standard drugs. (b) log 10 (reduction in colony forming units) after 1 and 4 days treatment with standard drugs

    Journal: Pharmacognosy Magazine

    Article Title: Allium sativum Constituents Exhibit Anti-tubercular Activity In vitro and in RAW 264.7 Mouse Macrophage Cells Infected with Mycobacterium tuberculosis H37Rv

    doi: 10.4103/pm.pm_435_16

    Figure Lengend Snippet: Anti-tubercular activity of thiosulfinate-derivative rich extract of garlic in comparison to standard drugs in 106 cells/well of RAW 264.7 macrophage cells infected with Mycobacterium tuberculosis H37Rv (5 × 106 colony forming unit/well). The results are expressed as mean ± standard deviation of three sets of experiments. After 1 and 4 days of incubation, the cells were lysed using 0.06% sodium dodecyl sulfate and plated on Middlebrook 7H11 agar plates after dilution in 1:10 ratio. (a) Number of colony forming units after 1 and 4 days treatment with extract and standard drugs. (b) log 10 (reduction in colony forming units) after 1 and 4 days treatment with standard drugs

    Article Snippet: The lysates were diluted in 1:10 ratio with sterile PBS and 10 μL was plated on Middlebrook 7H11 agar (Difco) supplemented with OADC.

    Techniques: Activity Assay, Infection, Standard Deviation, Incubation

    Comparison of the colony morphology of the M. tuberculosis wild type, the M. tuberculosis Δ pykA knockout mutant, the knockout complemented with pLK102 containing the pykA gene, and M. bovis . The strains are arranged from left to right, each grown on Middlebrook 7H11 plates with glycerol plus OADC (g), OADC only (o), and pyruvate plus OADC (p).

    Journal: Journal of Bacteriology

    Article Title: Global Effects of Inactivation of the Pyruvate Kinase Gene in the Mycobacterium tuberculosis Complex ▿ Complex ▿ †

    doi: 10.1128/JB.00619-09

    Figure Lengend Snippet: Comparison of the colony morphology of the M. tuberculosis wild type, the M. tuberculosis Δ pykA knockout mutant, the knockout complemented with pLK102 containing the pykA gene, and M. bovis . The strains are arranged from left to right, each grown on Middlebrook 7H11 plates with glycerol plus OADC (g), OADC only (o), and pyruvate plus OADC (p).

    Article Snippet: For colony morphology examination, Middlebrook 7H11 medium (Difco) containing 10% (vol/vol) oleic acid-ADC (OADC; Difco) enrichment with or without 0.5% glycerol was used.

    Techniques: Knock-Out, Mutagenesis

    A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on Middlebrook 7H11-enriched media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.

    Journal: PLoS ONE

    Article Title: Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans

    doi: 10.1371/journal.pone.0124626

    Figure Lengend Snippet: A . gracilis and C . calamistratum extracts increase the growth rate of M . marinum on Middlebrook 7H11-enriched media. Kinetics of growth of M . marinum in Middlebrook 7H11 medium and Middlebrook 7H11-based solid medium enriched with plant filtered extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent medium facilitating a significant increase of the number of M . marinum colonies at each time point compared to Middlebrook 7H11 medium.

    Article Snippet: The delay of detection of the first M . ulcerans colony was 8 ± 0 days on Middlebrook 7H11, 6.34 ± 0.75 days on A . gracilis -enriched medium (p < 0.01), 6 ± 1 days on E . africanus - and V . torta -enriched media (p < 0.01), 6 ± 0 days on V . nana -enriched medium (p < 0.01), and 5.67 ± 0.47 days on C . calamistratum -enriched medium (p < 0.01).

    Techniques:

    C . calamistratum and E . africanus extracts increase the growth rate of M . ulcerans on Middlebrook 7H11-enriched media. Kinetics of growth of M . ulcerans in Middlebrook 7H11 medium and Middlebrook 7H11-based solid media enriched with tropical plant extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent media facilitating a significant increase of the number of M . ulcerans colonies at each time point compared to Middlebrook 7H11 medium.

    Journal: PLoS ONE

    Article Title: Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans

    doi: 10.1371/journal.pone.0124626

    Figure Lengend Snippet: C . calamistratum and E . africanus extracts increase the growth rate of M . ulcerans on Middlebrook 7H11-enriched media. Kinetics of growth of M . ulcerans in Middlebrook 7H11 medium and Middlebrook 7H11-based solid media enriched with tropical plant extracts (10%, vol:vol), detected by autofluorescence. Each data point represents the mean ± standard error for six plates per time point. The asterisks represent media facilitating a significant increase of the number of M . ulcerans colonies at each time point compared to Middlebrook 7H11 medium.

    Article Snippet: The delay of detection of the first M . ulcerans colony was 8 ± 0 days on Middlebrook 7H11, 6.34 ± 0.75 days on A . gracilis -enriched medium (p < 0.01), 6 ± 1 days on E . africanus - and V . torta -enriched media (p < 0.01), 6 ± 0 days on V . nana -enriched medium (p < 0.01), and 5.67 ± 0.47 days on C . calamistratum -enriched medium (p < 0.01).

    Techniques:

    The antituberculosis drug Isoniazid suppresses host immunity by inducing activation-induced cell death in T cells. a , lung and spleen cfu. Mice infected with a high dose aerosol inoculum (∼150 cfu/mice) of M. tuberculosis strain H37Rv were sacrificed at various time points. Lungs and spleens were harvested, homogenized in 0.2 μ m filtered PBS containing 0.05% Tween 80, and plated onto 7H11 Middlebrook plates containing 10% oleic acid, albumin, dextrose, and catalase. Undiluted, 10-fold, 100-fold, and 1000-fold diluted lung and spleen cell homogenates were plated in duplicate on the above 7H11 agar plates and incubated at 37 °C for 21 days. Colonies were counted, and cfu were estimated. Data shown here are representative of five independent experiments. Each cfu experiment has been carried out with three mice per experiment. b , left panel , total number of splenocytes 30 days post-treatment. Total numbers of splenocytes were counted using a hemocytometer after preparation of single cell suspensions following isolation of spleens from mice. Data shown here are representative of five independent experiments with three mice in each group and represent the mean ± S.D. values. Right panel , the percentage of CD4 + T cells in lungs. Lung lymphocytes were stained with anti-CD4 antibodies, and data were acquired by flow cytometry. The percentage of cells expressing CD4 is shown in the bar diagram with mean ± S.D. Data shown here are representative of five independent experiments with three mice in each group. c , antigen-specific T cell proliferation. T lymphocytes were isolated from spleens of mice 30 days post-treatment with INH, and T cell proliferation assays were performed using tritiated thymidine after stimulation with M. tuberculosis H37Rv CSA. Data shown here are representative of five independent experiments with three mice in each group and represent the mean ± S.D. values. d , in vivo T cell proliferation in spleen and lung. To determine the status of host T cell proliferation in vivo during infection and treatment, 0.6 mg of BrdU in 100 μl of PBS was administered intraperitoneally to each mouse at 3 days prior to sacrifice. Cells were then isolated from both lung tissue and spleen and stained with anti-BrdU antibodies. Data were acquired by flow cytometry. The percentage of cells showing BrdU incorporation in different groups at 30 days post-treatment is shown in the dot-plot diagram with mean ± S.D. Data shown here are representative of five independent experiments with three mice in each group. e , lung cfu. Mice infected with a high dose aerosol inoculum (∼150 cfu/mice) of the BND320 strain of M. tuberculosis were sacrificed at various time points, and lungs were harvested, homogenized in 0.2 μ m filtered PBS containing 0.05% Tween 80, and plated onto 7H11 Middlebrook plates containing 10% oleic acid, albumin, dextrose, and catalase. Undiluted, 10-fold, 100-fold, and 1000-fold diluted lung cell homogenates were plated in duplicate on the above 7H11 plates and incubated at 37 °C for 21 days. Colonies were counted, and cfu were estimated. Data shown here are representative of three independent experiments. Each cfu experiment has been carried out with three mice per experiment. f , left panel , total number of splenocytes 45 days post-treatment. Total numbers of splenocytes were counted using a hemocytometer after preparation of single cell suspensions. Data shown here are representative of two independent experiments with three mice in each group and represent the mean ± S.D. values. Middle and right panels , the percentage of CD4 + and CD8 + T cells in spleen. Splenocytes were stained with anti-CD4 and anti-CD8 antibodies, and data were acquired by flow cytometry. The percentage of cells expressing CD4 or CD8 is shown in the bar diagram with mean ± S.D. at 30 and 45 days post-treatment. Data shown here are representative of three independent experiments with three mice in each group. g , CD69, CD44, and CD25 expression. Splenocytes of mice infected with BND320 and treated with INH for 45 days were surface-stained with anti-CD4, -CD69, -CD44, and -CD25 antibodies, and samples were acquired by flow cytometry. T cells were gated for CD4 expression, and the percentage of cells expressing CD69, CD44, and CD25 among CD4 + T cells is shown in the FACS plots with mean ± S.D. Data shown here are representative of three independent experiments with three mice in each group.

    Journal: The Journal of Biological Chemistry

    Article Title: Isoniazid Induces Apoptosis Of Activated CD4+ T Cells

    doi: 10.1074/jbc.C114.598946

    Figure Lengend Snippet: The antituberculosis drug Isoniazid suppresses host immunity by inducing activation-induced cell death in T cells. a , lung and spleen cfu. Mice infected with a high dose aerosol inoculum (∼150 cfu/mice) of M. tuberculosis strain H37Rv were sacrificed at various time points. Lungs and spleens were harvested, homogenized in 0.2 μ m filtered PBS containing 0.05% Tween 80, and plated onto 7H11 Middlebrook plates containing 10% oleic acid, albumin, dextrose, and catalase. Undiluted, 10-fold, 100-fold, and 1000-fold diluted lung and spleen cell homogenates were plated in duplicate on the above 7H11 agar plates and incubated at 37 °C for 21 days. Colonies were counted, and cfu were estimated. Data shown here are representative of five independent experiments. Each cfu experiment has been carried out with three mice per experiment. b , left panel , total number of splenocytes 30 days post-treatment. Total numbers of splenocytes were counted using a hemocytometer after preparation of single cell suspensions following isolation of spleens from mice. Data shown here are representative of five independent experiments with three mice in each group and represent the mean ± S.D. values. Right panel , the percentage of CD4 + T cells in lungs. Lung lymphocytes were stained with anti-CD4 antibodies, and data were acquired by flow cytometry. The percentage of cells expressing CD4 is shown in the bar diagram with mean ± S.D. Data shown here are representative of five independent experiments with three mice in each group. c , antigen-specific T cell proliferation. T lymphocytes were isolated from spleens of mice 30 days post-treatment with INH, and T cell proliferation assays were performed using tritiated thymidine after stimulation with M. tuberculosis H37Rv CSA. Data shown here are representative of five independent experiments with three mice in each group and represent the mean ± S.D. values. d , in vivo T cell proliferation in spleen and lung. To determine the status of host T cell proliferation in vivo during infection and treatment, 0.6 mg of BrdU in 100 μl of PBS was administered intraperitoneally to each mouse at 3 days prior to sacrifice. Cells were then isolated from both lung tissue and spleen and stained with anti-BrdU antibodies. Data were acquired by flow cytometry. The percentage of cells showing BrdU incorporation in different groups at 30 days post-treatment is shown in the dot-plot diagram with mean ± S.D. Data shown here are representative of five independent experiments with three mice in each group. e , lung cfu. Mice infected with a high dose aerosol inoculum (∼150 cfu/mice) of the BND320 strain of M. tuberculosis were sacrificed at various time points, and lungs were harvested, homogenized in 0.2 μ m filtered PBS containing 0.05% Tween 80, and plated onto 7H11 Middlebrook plates containing 10% oleic acid, albumin, dextrose, and catalase. Undiluted, 10-fold, 100-fold, and 1000-fold diluted lung cell homogenates were plated in duplicate on the above 7H11 plates and incubated at 37 °C for 21 days. Colonies were counted, and cfu were estimated. Data shown here are representative of three independent experiments. Each cfu experiment has been carried out with three mice per experiment. f , left panel , total number of splenocytes 45 days post-treatment. Total numbers of splenocytes were counted using a hemocytometer after preparation of single cell suspensions. Data shown here are representative of two independent experiments with three mice in each group and represent the mean ± S.D. values. Middle and right panels , the percentage of CD4 + and CD8 + T cells in spleen. Splenocytes were stained with anti-CD4 and anti-CD8 antibodies, and data were acquired by flow cytometry. The percentage of cells expressing CD4 or CD8 is shown in the bar diagram with mean ± S.D. at 30 and 45 days post-treatment. Data shown here are representative of three independent experiments with three mice in each group. g , CD69, CD44, and CD25 expression. Splenocytes of mice infected with BND320 and treated with INH for 45 days were surface-stained with anti-CD4, -CD69, -CD44, and -CD25 antibodies, and samples were acquired by flow cytometry. T cells were gated for CD4 expression, and the percentage of cells expressing CD69, CD44, and CD25 among CD4 + T cells is shown in the FACS plots with mean ± S.D. Data shown here are representative of three independent experiments with three mice in each group.

    Article Snippet: Calculation of Colony-forming Units Mice were sacrificed at the required time points, and organs were harvested, homogenized in 0.2 μm filtered PBS containing 0.05% Tween 80, and plated onto 7H11 Middlebrook (DifcoTM ) plates containing 10% oleic acid, albumin, dextrose, and catalase (DifcoTM ).

    Techniques: Activation Assay, Mouse Assay, Infection, Incubation, Isolation, Staining, Flow Cytometry, Cytometry, Expressing, In Vivo, BrdU Incorporation Assay, FACS