Journal: Frontiers in Microbiology
Article Title: Mycobacterial Cultures Contain Cell Size and Density Specific Sub-populations of Cells with Significant Differential Susceptibility to Antibiotics, Oxidative and Nitrite Stress
Figure Lengend Snippet: Panels from Video S1 on the live cell imaging of Msm short cells generating micro colony and population regeneration potential of the cells in the SCF and NCF . (A) An Msm cell elongates and divides symmetrically to generate two short daughter cells. One of the short daughter cells (green), grew and divided asymmetrically to generate two unequal-sized daughter cells (indicated in cyan and pink). The other short daughter cell (red), grew and divided symmetrically to generate two comparably-sized short daughter cells (indicated in white and yellow). The daughter cells from the earlier asymmetric division (cyan) further divided symmetrically to generate two daughter cells. The short daughter cell (yellow) further grew and divided symmetrically to generate two daughter cells. Arrows show the site of constriction. Cells of length ≤ 2.60 ± 0.25 μm were considered short cells (as per Figure 1B ). (B) Fraction enriched for Msm short cells. (C) Cells from mid-log phase population. (D) Population generated from the short-cells-enriched fraction after reinoculation into Middlebrook 7H9 liquid medium. (E) Population generated from plating of the cells in the short-cells-enriched fraction on Middlebrook 7H10 agar. The compositions of the cells in (D,E) are similar to that in (C) . Arrows indicate short cells. Length of Msm short cells are shown in (B) . (F–I) Acid-fast stained cells in the SCF and NCF and the respective population generated from them after reinoculation into Middlebrook 7H9 media and incubated till the cultures reached 0.6 OD 600nm .
Article Snippet: Determination of the regeneration potential, heat sensitivity, and viability of SCs and NCs The cells from SCF1 and SCF2 mixture and from NCF were independently inoculated into fresh Middlebrook 7H9 medium as well as plated on Middlebrook 7H10 agar medium, and examined the cells from MLP culture and from colonies from the plates, under microscope (DIC).
Techniques: Live Cell Imaging, Generated, Staining, Incubation