middlebrook 7h10 medium Search Results


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  • 99
    Thermo Fisher middlebrook 7h10 medium
    Middlebrook 7h10 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook mb 7h10
    Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto <t>Middlebrook</t> <t>7H10</t> (p
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    Difco middlebrook 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
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    Becton Dickinson middlebrook 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    Middlebrook 7h10 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories middlebrook 7h10 medium
    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on <t>Middlebrook</t> <t>7H10</t> agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P
    Middlebrook 7h10 Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h10 medium
    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    Difco middlebrook 7h10 plates
    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    Difco middlebrook 7h10 agar
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> <t>7H10</t> solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
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    MiddleBrook Pharmaceuticals middlebrook 7h10 7h11 medium
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> <t>7H10</t> solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Middlebrook 7h10 7h11 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals minimal medium middlebrook 7h10
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> <t>7H10</t> solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
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    MiddleBrook Pharmaceuticals solid middlebrook 7h10 medium
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> <t>7H10</t> solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Solid Middlebrook 7h10 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals nacl middlebrook 7h10 medium
    M. bolettii colonies on <t>7H10,</t> 7H10-1 <t>%-NaCl,</t> 7H10-2 %-NaCl and 7H10-3 %-NaCl
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    MiddleBrook Pharmaceuticals standard middlebrook 7h10 medium
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    MiddleBrook Pharmaceuticals reference middlebrook 7h10 medium
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    MiddleBrook Pharmaceuticals control middlebrook 7h10 medium
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    MiddleBrook Pharmaceuticals middlebrook 7h10 oadc medium
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    Difco middlebrooks 7h10 agar medium
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    MiddleBrook Pharmaceuticals oadc supplemented middlebrook 7h10 agar medium
    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    Modified <t>Middlebrook</t> <t>7H10</t> medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils
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    MiddleBrook Pharmaceuticals n acetyl galactosamine enriched middlebrook 7h10 medium
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    Becton Dickinson mb 7h10 agar
    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    MiddleBrook Pharmaceuticals tween less middlebrook 7h10 broth
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    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    Growth of M. ulcerans (10 4 bacilli) onto <t>Middlebrook</t> <t>7H10</t> enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.
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    Difco 7h10 solid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
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    Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Article Snippet: We propose that incubation of chlorhexidine-decontaminated environmental specimens on Middlebrook 7H10-enriched medium under micro-aerophilic atmosphere at 30 °C may be used for the tentative isolation of M. ulcerans strains from potential environmental sources.

    Techniques:

    Growth count at day 30 of 10 4 bacilli of M. ulcerans CU001 and M. ulcerans ATCC33728 colonies after 1% chlorhexidine decontamination of river freshwater artificially inoculated with M. ulcerans strains and cultured onto growth promoters and Middlebrook 7H10. Blue column: Mycobacterium ulcerans CU001; Red column: Mycobacterium ulcerans ATCC33728.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Growth count at day 30 of 10 4 bacilli of M. ulcerans CU001 and M. ulcerans ATCC33728 colonies after 1% chlorhexidine decontamination of river freshwater artificially inoculated with M. ulcerans strains and cultured onto growth promoters and Middlebrook 7H10. Blue column: Mycobacterium ulcerans CU001; Red column: Mycobacterium ulcerans ATCC33728.

    Article Snippet: We propose that incubation of chlorhexidine-decontaminated environmental specimens on Middlebrook 7H10-enriched medium under micro-aerophilic atmosphere at 30 °C may be used for the tentative isolation of M. ulcerans strains from potential environmental sources.

    Techniques: Cell Culture

    Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Article Snippet: We propose that incubation of chlorhexidine-decontaminated environmental specimens on Middlebrook 7H10-enriched medium under micro-aerophilic atmosphere at 30 °C may be used for the tentative isolation of M. ulcerans strains from potential environmental sources.

    Techniques:

    Culturing one aulacode feces sample collected in Côte d ’ Ivoire on Middlebrook 7H10 enriched with growth promoters yielded one micro-colony observed by autofluorescence (arrow) (left panel). Right panel exhibits Ziehl-Neelsen staining of the micro colony further identified as M. ulcerans by positive RT-qPCR for KR-B gene, IS 2404 and IS2606.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Culturing one aulacode feces sample collected in Côte d ’ Ivoire on Middlebrook 7H10 enriched with growth promoters yielded one micro-colony observed by autofluorescence (arrow) (left panel). Right panel exhibits Ziehl-Neelsen staining of the micro colony further identified as M. ulcerans by positive RT-qPCR for KR-B gene, IS 2404 and IS2606.

    Article Snippet: We propose that incubation of chlorhexidine-decontaminated environmental specimens on Middlebrook 7H10-enriched medium under micro-aerophilic atmosphere at 30 °C may be used for the tentative isolation of M. ulcerans strains from potential environmental sources.

    Techniques: Staining, Quantitative RT-PCR

    Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Article Snippet: We propose that incubation of chlorhexidine-decontaminated environmental specimens on Middlebrook 7H10-enriched medium under micro-aerophilic atmosphere at 30 °C may be used for the tentative isolation of M. ulcerans strains from potential environmental sources.

    Techniques: Concentration Assay

    Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Article Snippet: We observed that the N-acetyl-galactosamine-enriched Middlebrook 7H10 medium and three growth-promoters-Middlebrook 7H10 medium yielded similar results indicative that both media could be used for tentative isolation of MPM including M. ulcerans from environmental sources.

    Techniques: Concentration Assay

    Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Article Snippet: We observed that the N-acetyl-galactosamine-enriched Middlebrook 7H10 medium and three growth-promoters-Middlebrook 7H10 medium yielded similar results indicative that both media could be used for tentative isolation of MPM including M. ulcerans from environmental sources.

    Techniques:

    Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Article Snippet: We observed that the N-acetyl-galactosamine-enriched Middlebrook 7H10 medium and three growth-promoters-Middlebrook 7H10 medium yielded similar results indicative that both media could be used for tentative isolation of MPM including M. ulcerans from environmental sources.

    Techniques:

    Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook 7H10 agar, and colonies

    Journal: Infection and Immunity

    Article Title: Individual Mycobacterium tuberculosis Resuscitation-Promoting Factor Homologues Are Dispensable for Growth In Vitro and In Vivo

    doi: 10.1128/IAI.72.1.515-526.2004

    Figure Lengend Snippet: Colony size of the original ΔRv1009 strain (mc 2 3126) compared with Erdman wild type and the effect on colony size of complementation with Rv1009 and with ksgA . Cultures were grown and transferred onto plates with Middlebrook 7H10 agar, and colonies

    Article Snippet: When plated onto Middlebrook 7H10 solid medium, each of the rpf deletion mutant strains displayed a colony morphology similar to wild type, with the exception of the Rv1009 disruption mutant strains, mc2 3126 and mc2 3126A.

    Techniques:

    Panels from Video S1 on the live cell imaging of Msm short cells generating micro colony and population regeneration potential of the cells in the SCF and NCF . (A) An Msm cell elongates and divides symmetrically to generate two short daughter cells. One of the short daughter cells (green), grew and divided asymmetrically to generate two unequal-sized daughter cells (indicated in cyan and pink). The other short daughter cell (red), grew and divided symmetrically to generate two comparably-sized short daughter cells (indicated in white and yellow). The daughter cells from the earlier asymmetric division (cyan) further divided symmetrically to generate two daughter cells. The short daughter cell (yellow) further grew and divided symmetrically to generate two daughter cells. Arrows show the site of constriction. Cells of length ≤ 2.60 ± 0.25 μm were considered short cells (as per Figure 1B ). (B) Fraction enriched for Msm short cells. (C) Cells from mid-log phase population. (D) Population generated from the short-cells-enriched fraction after reinoculation into Middlebrook 7H9 liquid medium. (E) Population generated from plating of the cells in the short-cells-enriched fraction on Middlebrook 7H10 agar. The compositions of the cells in (D,E) are similar to that in (C) . Arrows indicate short cells. Length of Msm short cells are shown in (B) . (F–I) Acid-fast stained cells in the SCF and NCF and the respective population generated from them after reinoculation into Middlebrook 7H9 media and incubated till the cultures reached 0.6 OD 600nm .

    Journal: Frontiers in Microbiology

    Article Title: Mycobacterial Cultures Contain Cell Size and Density Specific Sub-populations of Cells with Significant Differential Susceptibility to Antibiotics, Oxidative and Nitrite Stress

    doi: 10.3389/fmicb.2017.00463

    Figure Lengend Snippet: Panels from Video S1 on the live cell imaging of Msm short cells generating micro colony and population regeneration potential of the cells in the SCF and NCF . (A) An Msm cell elongates and divides symmetrically to generate two short daughter cells. One of the short daughter cells (green), grew and divided asymmetrically to generate two unequal-sized daughter cells (indicated in cyan and pink). The other short daughter cell (red), grew and divided symmetrically to generate two comparably-sized short daughter cells (indicated in white and yellow). The daughter cells from the earlier asymmetric division (cyan) further divided symmetrically to generate two daughter cells. The short daughter cell (yellow) further grew and divided symmetrically to generate two daughter cells. Arrows show the site of constriction. Cells of length ≤ 2.60 ± 0.25 μm were considered short cells (as per Figure 1B ). (B) Fraction enriched for Msm short cells. (C) Cells from mid-log phase population. (D) Population generated from the short-cells-enriched fraction after reinoculation into Middlebrook 7H9 liquid medium. (E) Population generated from plating of the cells in the short-cells-enriched fraction on Middlebrook 7H10 agar. The compositions of the cells in (D,E) are similar to that in (C) . Arrows indicate short cells. Length of Msm short cells are shown in (B) . (F–I) Acid-fast stained cells in the SCF and NCF and the respective population generated from them after reinoculation into Middlebrook 7H9 media and incubated till the cultures reached 0.6 OD 600nm .

    Article Snippet: Determination of the regeneration potential, heat sensitivity, and viability of SCs and NCs The cells from SCF1 and SCF2 mixture and from NCF were independently inoculated into fresh Middlebrook 7H9 medium as well as plated on Middlebrook 7H10 agar medium, and examined the cells from MLP culture and from colonies from the plates, under microscope (DIC).

    Techniques: Live Cell Imaging, Generated, Staining, Incubation

    Mutations within HadA or HadC confer growth resistance during ISO treatment. Mid-log-phase cultures of wild-type (WT) M. tuberculosis and strains carrying either the C61G mutation in HadA (MTTR3) or the V85F mutation in HadC (MTTR18) were streaked onto Middlebrook 7H10 OADC plates supplemented with increasing concentrations of ISO (1, 2.5, and 5 μg/ml). Plates were incubated for 2 to 3 weeks at 37°C, after which growth was visualized.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Point Mutations within the Fatty Acid Synthase Type II Dehydratase Components HadA or HadC Contribute to Isoxyl Resistance in Mycobacterium tuberculosis

    doi: 10.1128/AAC.01972-12

    Figure Lengend Snippet: Mutations within HadA or HadC confer growth resistance during ISO treatment. Mid-log-phase cultures of wild-type (WT) M. tuberculosis and strains carrying either the C61G mutation in HadA (MTTR3) or the V85F mutation in HadC (MTTR18) were streaked onto Middlebrook 7H10 OADC plates supplemented with increasing concentrations of ISO (1, 2.5, and 5 μg/ml). Plates were incubated for 2 to 3 weeks at 37°C, after which growth was visualized.

    Article Snippet: The MICs of ISO and TAC were determined on Middlebrook 7H10 OADC medium containing 20 μg/ml pantothenic acid and increasing drug concentrations.

    Techniques: Mutagenesis, Incubation

    BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on Middlebrook 7H10 agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P

    Journal: BMC Immunology

    Article Title: Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection

    doi: 10.1186/s12865-018-0258-8

    Figure Lengend Snippet: BCG infection kinetics of murine LN DCs. Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU rBCG-GFP, and LN cells were harvested at different time points. DCs were sorted and analyzed for the presence of rBCG-GFP. Infection of murine LN DCs with BCG ( a ). Six groups of mice ( n = 6) were s.c. injected with 1 × 10 8 CFU BCG, and BCG in DCs was quantified in CFUs by culturing on Middlebrook 7H10 agar ( b ). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t -test (* P

    Article Snippet: Both strains were grown with gentle agitation (80 rpm) in Middlebrook 7H9 medium (Difco, Detroit, MI, USA) supplemented with 0.05% Tween 80 and 10% albumin-dextrose-catalase (ADC) enrichment or on solid Middlebrook 7H10 medium (Difco) supplemented with 0.05% Tween 80 and 10% oleic-ADC enrichment.

    Techniques: Infection, Mouse Assay, Injection

    Genotypes and morphologies of BCG Tokyo 172 type I and II subpopulations. A , the colony morphology was observed by an optical microscope after incubation for 2 weeks on 7H10 agar plates. Type I was smooth, irregular, and flat, and type II was rough, raised,

    Journal: The Journal of Biological Chemistry

    Article Title: Lipid Phenotype of Two Distinct Subpopulations of Mycobacterium bovis Bacillus Calmette-Gu?rin Tokyo 172 Substrain *

    doi: 10.1074/jbc.M111.310037

    Figure Lengend Snippet: Genotypes and morphologies of BCG Tokyo 172 type I and II subpopulations. A , the colony morphology was observed by an optical microscope after incubation for 2 weeks on 7H10 agar plates. Type I was smooth, irregular, and flat, and type II was rough, raised,

    Article Snippet: BCG Tokyo 172 subpopulation types I and II were isolated on Middlebrook 7H10 agar medium (Difco) with 0.5% glycerol and 10% Middlebrook oleic acid-albumin-dextrose-catalase enrichment (Difco) at 37 °C for 2 weeks ( ).

    Techniques: Microscopy, Incubation

    Modified Middlebrook 7H10 medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils

    Journal: AMB Express

    Article Title: Isolation of nontuberculous mycobacteria from soil using Middlebrook 7H10 agar with increased malachite green concentration

    doi: 10.1186/s13568-017-0373-6

    Figure Lengend Snippet: Modified Middlebrook 7H10 medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils

    Article Snippet: Colonies grown on the selective medium were selected based on their growth rate, morphology, and pigmentation and then streaked onto new modified Middlebrook 7H10 medium containing 250 mg/L MG using a calibrated loop to repurify the isolates.

    Techniques: Modification, Isolation

    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Journal: Frontiers in Microbiology

    Article Title: Functional Studies of Five Toxin-Antitoxin Modules in Mycobacterium tuberculosis H37Rv

    doi: 10.3389/fmicb.2016.02071

    Figure Lengend Snippet: Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Article Snippet: M. smegmatis mc2 155 was grown in Middlebrook 7H9 (a liquid medium, Difco) containing 0.2% glycerol and 0.02% Tween 80 or Middlebrook 7H10 (a solid agar medium, Difco) containing 0.2% glycerol.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation

    M. bolettii colonies on 7H10, 7H10-1 %-NaCl, 7H10-2 %-NaCl and 7H10-3 %-NaCl

    Journal: BMC Research Notes

    Article Title: Inverse correlation between salt tolerance and host-adaptation in mycobacteria

    doi: 10.1186/s13104-016-2054-y

    Figure Lengend Snippet: M. bolettii colonies on 7H10, 7H10-1 %-NaCl, 7H10-2 %-NaCl and 7H10-3 %-NaCl

    Article Snippet: As for M. bolettii , the size of colonies increased from 1.16 ± 0.4 mm in the Middlebrook 7H10 control medium up to 2.95 ± 0.9 mm in 3 % NaCl Middlebrook 7H10 medium (P < 0.05, student’s t test), then decreased down to 0.4 ± 0.2 mm in 7 % NaCl Middlebrook 7H10 medium (P < 0.05, student’s t test) (Fig. ; Table ).

    Techniques:

    Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Article Snippet: The negative control was consisted of seeding 100 µL of sterile PBS onto 5% sheep-blood Columbia agar, standard Middlebrook 7H10 medium and onto each of the growth-promoting media.

    Techniques: Concentration Assay

    Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Article Snippet: The negative control was consisted of seeding 100 µL of sterile PBS onto 5% sheep-blood Columbia agar, standard Middlebrook 7H10 medium and onto each of the growth-promoting media.

    Techniques:

    Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Article Snippet: The negative control was consisted of seeding 100 µL of sterile PBS onto 5% sheep-blood Columbia agar, standard Middlebrook 7H10 medium and onto each of the growth-promoting media.

    Techniques:

    Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Article Snippet: The doubling time of M. ulcerans during the exponential growth was measured at 1.65 ± 0.01 days with chitin, 1.74 ± 0.03 days with N-acetyl-galactosamine, 1.75 ± 0.01 days for fucose, 1.68 ± 0.05 days with chitin/N-acetyl-galactosamine/fucose and 2.40 ± 0.58 days with the control Middlebrook 7H10 medium.

    Techniques: Concentration Assay

    Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Article Snippet: The doubling time of M. ulcerans during the exponential growth was measured at 1.65 ± 0.01 days with chitin, 1.74 ± 0.03 days with N-acetyl-galactosamine, 1.75 ± 0.01 days for fucose, 1.68 ± 0.05 days with chitin/N-acetyl-galactosamine/fucose and 2.40 ± 0.58 days with the control Middlebrook 7H10 medium.

    Techniques:

    Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Article Snippet: The doubling time of M. ulcerans during the exponential growth was measured at 1.65 ± 0.01 days with chitin, 1.74 ± 0.03 days with N-acetyl-galactosamine, 1.75 ± 0.01 days for fucose, 1.68 ± 0.05 days with chitin/N-acetyl-galactosamine/fucose and 2.40 ± 0.58 days with the control Middlebrook 7H10 medium.

    Techniques:

    Modified Middlebrook 7H10 medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils

    Journal: AMB Express

    Article Title: Isolation of nontuberculous mycobacteria from soil using Middlebrook 7H10 agar with increased malachite green concentration

    doi: 10.1186/s13568-017-0373-6

    Figure Lengend Snippet: Modified Middlebrook 7H10 medium containing 250 mg/L MG with mycobacteria primarily isolated from soil. a Sample collected at campus, b sample collected at community, and c collected at natural park. Black arrows indicate mycobacterial colonies, red arrows indicate dried soils

    Article Snippet: Isolation of mycobacteria from soil samples Modified Middlebrook 7H10 medium containing 250 mg/L MG was used to isolate mycobacteria from 116 soil samples.

    Techniques: Modification, Isolation

    Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Growth of M. ulcerans (10 4 bacilli) onto Middlebrook 7H10 enriched with high concentration of N-acethyl galactosamine (0.1 mg/mL, 0.5 mg/mL) and fucose (0.1 mg/mL, 0.5 ( A ). M. ulcerans CU001; ( B ) M. ulcerans ATCC25900; ( C ) M. ulcerans ATCC33728.

    Article Snippet: We observed that the N-acetyl-galactosamine-enriched Middlebrook 7H10 medium and three growth-promoters-Middlebrook 7H10 medium yielded similar results indicative that both media could be used for tentative isolation of MPM including M. ulcerans from environmental sources.

    Techniques: Concentration Assay

    Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Improved growth of M. ulcerans strains (10 4 bacilli) by N-acetyl galactosamine (NAG) (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) and DZ medium (NAG (0.01 mg/mL), fucose (0.01 mg/mL), chitin (0.2 mg/mL) versus growth onto Middlebrook 7H10 (p

    Article Snippet: We observed that the N-acetyl-galactosamine-enriched Middlebrook 7H10 medium and three growth-promoters-Middlebrook 7H10 medium yielded similar results indicative that both media could be used for tentative isolation of MPM including M. ulcerans from environmental sources.

    Techniques:

    Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Journal: Scientific Reports

    Article Title: A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans

    doi: 10.1038/s41598-018-25278-y

    Figure Lengend Snippet: Triplicate culture of 10 4 bacilli of M. ulcerans CU001 ( A ), M. ulcerans ATCC25900 ( B ) and M. ulcerans ATCC33728 ( C ) in Middlebrook 7H10 under different atmospheres (ambient atmosphere, and microaerophilic, anaerobic and 5% CO 2 -enriched atmosphere). Microaerophilic atmosphere yielded the optimal growth of M. ulcerans (red line).

    Article Snippet: We observed that the N-acetyl-galactosamine-enriched Middlebrook 7H10 medium and three growth-promoters-Middlebrook 7H10 medium yielded similar results indicative that both media could be used for tentative isolation of MPM including M. ulcerans from environmental sources.

    Techniques:

    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Journal: Scientific Reports

    Article Title: Assessing the role of Rv1222 (RseA) as an anti-sigma factor of the Mycobacterium tuberculosis extracytoplasmic sigma factor SigE

    doi: 10.1038/s41598-019-41183-4

    Figure Lengend Snippet: Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Article Snippet: M. tuberculosis H37Rv was grown in either Middlebrook 7H9 liquid medium or Middlebrook 7H10 solid medium (Difco) supplemented with 0.05% Tween 80 and ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques: Mutagenesis, Incubation