Journal: PLoS ONE

Article Title: Methamphetamine administration increases hepatic CYP1A2 but not CYP3A activity in female guinea pigs

doi: 10.1371/journal.pone.0233010

Figure Lengend Snippet: Test drugs caffeine (metabolised by CYP1A2) and midazolam (metabolised by CYP3A) was administered 2 hours after the methamphetamine dose and plasma samples were collected 3, 4, 5, 6 and 8 hours after methamphetamine dose. Error bars represent standard error of mean (SEM).

Article Snippet: Acetonitrile (Honeywell Australia, North Ryde, Australia); caffeine citrate (Biomed Limited, Auckland, New Zealand); internal standard caffeine d3 (Toronto Research Chemicals, Toronto, Canada); Formic acid (Sigma Aldrich, New South Wales, Australia); isoflurane (Attane TM , Bayer, Auckland, New Zealand); KiCqStart SYBR Green qPCR ReadyMix Low Rox (Sigma Aldrich, New South Wales, Australia); methamphetamine (BDG Synthesis, Wellington, New Zealand); internal standard methamphetamine d5 (Toronto Research Chemicals, Toronto, Canada); midazolam (Claris, AFT Pharmaceutical Limited, Auckland, New Zealand); internal standard midazolam d6 (Toronto Research Chemicals, Toronto, Canada); internal standard paraxanthine d3 (Toronto Research Chemicals, Toronto, Canada); QIAZOL Lysis Reagent (QIAGEN, Victoria); RNeasy Mini Kit (QIAGEN, Victoria); sodium pentobarbital (Pentobarb, Provet, Palmerston North, New Zealand).

Techniques: Clinical Proteomics

Journal: bioRxiv

Article Title: 3D Spheroid Primary Human Hepatocytes for Prediction of Cytochrome P450 and Drug Transporter Induction

doi: 10.1101/2022.10.30.514199

Figure Lengend Snippet: Spheroids were treated with inducers or vehicle (0.1% DMSO) or were untreated (No treatment) for 96 hours before incubation with the Basel cocktail (10 μM midazolam, 30 μM omeprazole, 20 μM efavirenz, 40 μM metoprolol, 30 μM losartan and 160 μM caffeine). The metabolic activities of CYP3A4, CYP2C19, CYP2B6 and CYP2D6 were measured from samples incubated for 8 hours or 0.5 hour in the case CYP2C19. Data were normalized to mean concentration of metabolites in the vehicle group and are presented as means of two hepatocyte donors. N = 2 hepatocyte donors each presented as a mean value of three biological replicates per condition. Dashed lines indicate a two-fold difference to the vehicle group.

Article Snippet: Omeprazole and efavirenz (Sigma-Aldrich) and midazolam (Toronto Research Chemicals, Toronto, ON, Canada) were dissolved in DMSO as 6,000x stocks.

Techniques: Incubation, Concentration Assay

Journal: Experimental Animals

Article Title: Physiological, pharmacokinetic and liver metabolism comparisons between 3-, 6-, 12- and 18-month-old male Sprague Dawley rats under ketamine-xylazine anesthesia

doi: 10.1538/expanim.15-0039

Figure Lengend Snippet: Kinetic parameters associated with the formation α-hydroxymidazolam and norketamine in liver S9 fractions from aging rats

Article Snippet: Ketamine, d 4 -ketamine, norketamine, d 4 -norketamine, midazolam, d 4 -midazolam, α-hydroxymidazolam, and d 4 -α-hydroxymidazolam were obtained in solution from Cerilliant (Round Rock, TX, USA).

Techniques: