microtiter plate reader Search Results


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  • 94
    Bio-Rad microtiter plate reader
    Reactivity of purified IgG antibodies against L. infantum chagasi antigen (LiPA). 10 μ g of affinity-purified IgGs from CVL dogs (●) and negative dogs (■) was added to each well (from 10 μ g to 0.625 μ g/well) of <t>microtiter</t> plates coated with 50 μ g/mL LiPA. The reaction was detected using a peroxidase conjugated anti-dog IgG antibody (1 : 2000). Absorbance values at 492 nm were means of duplicates.
    Microtiter Plate Reader, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson microtiter plate reader
    Reactivity of purified IgG antibodies against L. infantum chagasi antigen (LiPA). 10 μ g of affinity-purified IgGs from CVL dogs (●) and negative dogs (■) was added to each well (from 10 μ g to 0.625 μ g/well) of <t>microtiter</t> plates coated with 50 μ g/mL LiPA. The reaction was detected using a peroxidase conjugated anti-dog IgG antibody (1 : 2000). Absorbance values at 492 nm were means of duplicates.
    Microtiter Plate Reader, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega microtiter plate reader
    Reactivity of purified IgG antibodies against L. infantum chagasi antigen (LiPA). 10 μ g of affinity-purified IgGs from CVL dogs (●) and negative dogs (■) was added to each well (from 10 μ g to 0.625 μ g/well) of <t>microtiter</t> plates coated with 50 μ g/mL LiPA. The reaction was detected using a peroxidase conjugated anti-dog IgG antibody (1 : 2000). Absorbance values at 492 nm were means of duplicates.
    Microtiter Plate Reader, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Tecan Systems microtiter plate reader
    Effect of gallium nitrate on bacterial proliferation. (A) Pseudomonas aeruginosa and (B) Streptococcus pyogenes were grown in 96-well microplates with medium containing gallium nitrate at concentrations ranging from 5 to 30 μ M. The plates were incubated at 37°C for 24 h without agitation. The turbidity of the medium was directly proportional to the growth of the bacteria, which was measured by a <t>microtiter</t> plate reader at 600 nm absorbance.
    Microtiter Plate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 93/100, based on 1368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioTek Instruments microtiter plate reader
    Se CobB L , Se CobB S , and Ea CobB L proteins restore growth of an S. enterica cobB mutant strain on low acetate. (A) Growth behavior of S. enterica on MOPS minimal medium supplemented with acetate (10 mM). Growth experiments were performed at 37°C using a <t>microtiter</t> plate and a microtiter plate reader (Bio-Tek Instruments). (B) CobB Western blot analysis of S. enterica cobB strains overproducing Se CobB L , Se CobB S , or CobB L/S during growth on acetate (10 mM). Se CobB L is CobB M37A M38A , encoded by cobB1372 ; Se CobB S is CobB M1A , encoded by cobB1373 .
    Microtiter Plate Reader, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 93/100, based on 2360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific microtiter plate reader
    Se CobB L , Se CobB S , and Ea CobB L proteins restore growth of an S. enterica cobB mutant strain on low acetate. (A) Growth behavior of S. enterica on MOPS minimal medium supplemented with acetate (10 mM). Growth experiments were performed at 37°C using a <t>microtiter</t> plate and a microtiter plate reader (Bio-Tek Instruments). (B) CobB Western blot analysis of S. enterica cobB strains overproducing Se CobB L , Se CobB S , or CobB L/S during growth on acetate (10 mM). Se CobB L is CobB M37A M38A , encoded by cobB1372 ; Se CobB S is CobB M1A , encoded by cobB1373 .
    Microtiter Plate Reader, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DYNEX tech microtiter plate reader
    Biofilm formation and EPS production by T. thermophilus HB27 strains. A , biofilm formation by strains of T. thermophilus HB27 overexpressing various PilF proteins. Biofilms formed on 96-well <t>microtiter</t> plates after 24 h were stained with crystal violet.
    Microtiter Plate Reader, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 92/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Packard Instruments microtiter plate reader
    Biofilm formation and EPS production by T. thermophilus HB27 strains. A , biofilm formation by strains of T. thermophilus HB27 overexpressing various PilF proteins. Biofilms formed on 96-well <t>microtiter</t> plates after 24 h were stained with crystal violet.
    Microtiter Plate Reader, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BMG Labtech microtiter plate reader
    Monospecies cariogenic biofilm biomass is influenced by carbohydrate source. Biomass was quantified spectrophotometrically by reading absorbance at 570 nm in a <t>microtiter</t> plate reader (FluoStar Omega, BMG Labtech). Four replicates were used for each isolate and was carried out on triplicate separate occasions, with the mean of each represented. Data represents mean with significance ∗ p
    Microtiter Plate Reader, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 92/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Dynatech Laboratories microtiter plate reader
    Monospecies cariogenic biofilm biomass is influenced by carbohydrate source. Biomass was quantified spectrophotometrically by reading absorbance at 570 nm in a <t>microtiter</t> plate reader (FluoStar Omega, BMG Labtech). Four replicates were used for each isolate and was carried out on triplicate separate occasions, with the mean of each represented. Data represents mean with significance ∗ p
    Microtiter Plate Reader, supplied by Dynatech Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Molecular Devices LLC microtiter plate reader
    Binding of CRP to immobilized proteins at acidic pH. Results of a protein ligand-binding assay are shown. <t>Microtiter</t> wells were coated with complement factor H, amyloid β, BSA, and gelatin. CRP (10 μg/ml), diluted in TBS-Ca (pH 7.0 to 5.0), was then added to the wells. Bound CRP was detected by using a rabbit anti-CRP antibody and HRP-conjugated donkey anti-rabbit IgG. The absorbance of the developed color was read at 405 nm. A representative of three experiments is shown.
    Microtiter Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 93/100, based on 2306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher microtiter plate reader
    Antibacterial activity of antibiotics in combination with the Trp-containing peptides. Bacterial cultures were treated with a series of concentration of antibiotics in the presence of a low peptide concentration equivalent to one-fourth of its MIC at 37°C overnight. The OD600 was recorded using a <t>microtiter</t> plate reader. The MIC was defined as the lowest antibiotics concentration that inhibited 95% of the bacterial growth. Each data point represents an average of six independent experiments.
    Microtiter Plate Reader, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Endress+Hauser microtiter plate reader
    Antibacterial activity of antibiotics in combination with the Trp-containing peptides. Bacterial cultures were treated with a series of concentration of antibiotics in the presence of a low peptide concentration equivalent to one-fourth of its MIC at 37°C overnight. The OD600 was recorded using a <t>microtiter</t> plate reader. The MIC was defined as the lowest antibiotics concentration that inhibited 95% of the bacterial growth. Each data point represents an average of six independent experiments.
    Microtiter Plate Reader, supplied by Endress+Hauser, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer microtiter plate reader
    Cross-species reactivity of clec14a-CTLD IgGs to human and mouse CTLDs. ELISA was performed with purified, selected IgG scFv clones (clones 1–4) on 96-well <t>microtiter</t> plates coated with hCTLD-Fc (▪), mCTLD-Fc (□) and Fc (□). BSA ( ) served as a background control. Values represent mean±s.d. of triplicate measurements from one of two independent experiments.
    Microtiter Plate Reader, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Berthold Technologies microtiter plate reader
    Cross-species reactivity of clec14a-CTLD IgGs to human and mouse CTLDs. ELISA was performed with purified, selected IgG scFv clones (clones 1–4) on 96-well <t>microtiter</t> plates coated with hCTLD-Fc (▪), mCTLD-Fc (□) and Fc (□). BSA ( ) served as a background control. Values represent mean±s.d. of triplicate measurements from one of two independent experiments.
    Microtiter Plate Reader, supplied by Berthold Technologies, used in various techniques. Bioz Stars score: 92/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beckman Coulter microtiter plate reader
    DNase I and Proteinase K inhibit nicotine-induced biofilm formation. The biofilm formation of S. aureus USA300 strain was detected using a <t>microtiter</t> plate assay by measuring crystal violet stained biofilm at OD570. DNase I (25 U/well) and Proteinase K (2 ug/ml) was added to the well in both 2 mg/ml nicotine treatment group and control group. ( A ) Treated by DNaseI, the average thickness of biofilms varied from 1.506 ± 0.04 to 0.450 ± 0.07 in the nicotine-treated group (n = 3) and from 0.720 ± 0.14 to 0.328 ± 0.05 in untreated group. (n = 3) ( B ) Proteinase K(2 ug/ml) disrupted biofilm formation, in the nicotine treated group decreasing from 1.300 ± 0.06 to 0.393 ± 0.02 (n = 3), and in the control group decreasing from 0.721 ± 0.14 to 0.316 ± 0.08 (n = 3). *P
    Microtiter Plate Reader, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Hitachi Ltd microtiter plate reader
    DNase I and Proteinase K inhibit nicotine-induced biofilm formation. The biofilm formation of S. aureus USA300 strain was detected using a <t>microtiter</t> plate assay by measuring crystal violet stained biofilm at OD570. DNase I (25 U/well) and Proteinase K (2 ug/ml) was added to the well in both 2 mg/ml nicotine treatment group and control group. ( A ) Treated by DNaseI, the average thickness of biofilms varied from 1.506 ± 0.04 to 0.450 ± 0.07 in the nicotine-treated group (n = 3) and from 0.720 ± 0.14 to 0.328 ± 0.05 in untreated group. (n = 3) ( B ) Proteinase K(2 ug/ml) disrupted biofilm formation, in the nicotine treated group decreasing from 1.300 ± 0.06 to 0.393 ± 0.02 (n = 3), and in the control group decreasing from 0.721 ± 0.14 to 0.316 ± 0.08 (n = 3). *P
    Microtiter Plate Reader, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Biochrom microtiter plate reader
    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp <t>microtiter</t> plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.
    Microtiter Plate Reader, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SouthernBiotech microtiter plate reader
    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp <t>microtiter</t> plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.
    Microtiter Plate Reader, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Turner BioSystems microtiter plate reader
    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp <t>microtiter</t> plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.
    Microtiter Plate Reader, supplied by Turner BioSystems, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Awareness Technology microtiter plate reader
    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp <t>microtiter</t> plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.
    Microtiter Plate Reader, supplied by Awareness Technology, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM microtiter plate reader
    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp <t>microtiter</t> plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.
    Microtiter Plate Reader, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Dainippon Sumitomo microtiter plate reader
    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp <t>microtiter</t> plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.
    Microtiter Plate Reader, supplied by Dainippon Sumitomo, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Reactivity of purified IgG antibodies against L. infantum chagasi antigen (LiPA). 10 μ g of affinity-purified IgGs from CVL dogs (●) and negative dogs (■) was added to each well (from 10 μ g to 0.625 μ g/well) of microtiter plates coated with 50 μ g/mL LiPA. The reaction was detected using a peroxidase conjugated anti-dog IgG antibody (1 : 2000). Absorbance values at 492 nm were means of duplicates.

    Journal: BioMed Research International

    Article Title: Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries

    doi: 10.1155/2015/401509

    Figure Lengend Snippet: Reactivity of purified IgG antibodies against L. infantum chagasi antigen (LiPA). 10 μ g of affinity-purified IgGs from CVL dogs (●) and negative dogs (■) was added to each well (from 10 μ g to 0.625 μ g/well) of microtiter plates coated with 50 μ g/mL LiPA. The reaction was detected using a peroxidase conjugated anti-dog IgG antibody (1 : 2000). Absorbance values at 492 nm were means of duplicates.

    Article Snippet: The resulting color was measured at 492 nm with an automated microtiter plate reader (Model 450, Bio-Rad).

    Techniques: Purification, Affinity Purification

    (a) Enrichment of phage binding after three rounds of panning. 50 μ L of a 10 10 transduction unit phage suspension collected after each round of panning was added to wells of microtiter plates coated with 5 μ g/mL anti-LiPA IgG. Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are means of duplicates. (b) Reactivity of individual clones isolated after panning 3. 50 μ L of a 10 10 transduction unit phage suspension isolated after the third round of panning was added to each well of microtiter plates coated with 1 μ g/well of anti- L. infantum chagasi IgGs (●) and anti- T. cruzi IgGs (■). Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are means of duplicates. (c) The aminoacid sequences of the three selected peptides.

    Journal: BioMed Research International

    Article Title: Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries

    doi: 10.1155/2015/401509

    Figure Lengend Snippet: (a) Enrichment of phage binding after three rounds of panning. 50 μ L of a 10 10 transduction unit phage suspension collected after each round of panning was added to wells of microtiter plates coated with 5 μ g/mL anti-LiPA IgG. Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are means of duplicates. (b) Reactivity of individual clones isolated after panning 3. 50 μ L of a 10 10 transduction unit phage suspension isolated after the third round of panning was added to each well of microtiter plates coated with 1 μ g/well of anti- L. infantum chagasi IgGs (●) and anti- T. cruzi IgGs (■). Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are means of duplicates. (c) The aminoacid sequences of the three selected peptides.

    Article Snippet: The resulting color was measured at 492 nm with an automated microtiter plate reader (Model 450, Bio-Rad).

    Techniques: Binding Assay, Transduction, Clone Assay, Isolation

    Effect of gallium nitrate on bacterial proliferation. (A) Pseudomonas aeruginosa and (B) Streptococcus pyogenes were grown in 96-well microplates with medium containing gallium nitrate at concentrations ranging from 5 to 30 μ M. The plates were incubated at 37°C for 24 h without agitation. The turbidity of the medium was directly proportional to the growth of the bacteria, which was measured by a microtiter plate reader at 600 nm absorbance.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Pre-treatment with EDTA-gallium prevents the formation of biofilms on surfaces

    doi: 10.3892/etm.2013.946

    Figure Lengend Snippet: Effect of gallium nitrate on bacterial proliferation. (A) Pseudomonas aeruginosa and (B) Streptococcus pyogenes were grown in 96-well microplates with medium containing gallium nitrate at concentrations ranging from 5 to 30 μ M. The plates were incubated at 37°C for 24 h without agitation. The turbidity of the medium was directly proportional to the growth of the bacteria, which was measured by a microtiter plate reader at 600 nm absorbance.

    Article Snippet: The biofilm mass was determined by measuring the absorbance at 590 nm using the microtiter plate reader.

    Techniques: Incubation

    Rifampicin resistance mutation assay. ( a , b ) S. pneumoniae D-PEP22 populations seeded in 44 microtitre plate wells at an inoculation density of OD 0.001 in the presence of 0.038 μg ml −1 RIF ( a ) and at an inoculation density of OD 0.02 in the presence of 0.38 μg ml −1 RIF ( b ), followed over time by measuring cell density (OD595) and gene expression (RLU; insets). Note that results are shown on linear scales, which more clearly display the emergence of resistant populations. ( c , d ) Number of resistant cells (CFUs ml −1 ) after plating pooled samples of 44 microtitre plate wells (75 μl sampling volume per well per time point) originating from 0.038 μg ml −1 rifampicin ( c ) and 0.38 μg ml −1 rifampicin ( d ) treatments (duplicate assays of the ones shown in a , b based on the same pre-culture) directly into 0, 0.38, and 3.8 μg ml −1 RIF, respectively; average and s.e.m. of duplicates are shown. ( e ) Sequencing of rpoB of 12 isolates originating from spontaneously resistant populations during 0.038-μg ml −1 RIF treatment, with the relevant RpoB amino-acid sequence (bold) together with the codons inside the dashed frame and their position in italics above; below, point mutations, the increase in resistance they generate and their frequency of occurrence are indicated.

    Journal: Nature Communications

    Article Title: Microscale insights into pneumococcal antibiotic mutant selection windows

    doi: 10.1038/ncomms9773

    Figure Lengend Snippet: Rifampicin resistance mutation assay. ( a , b ) S. pneumoniae D-PEP22 populations seeded in 44 microtitre plate wells at an inoculation density of OD 0.001 in the presence of 0.038 μg ml −1 RIF ( a ) and at an inoculation density of OD 0.02 in the presence of 0.38 μg ml −1 RIF ( b ), followed over time by measuring cell density (OD595) and gene expression (RLU; insets). Note that results are shown on linear scales, which more clearly display the emergence of resistant populations. ( c , d ) Number of resistant cells (CFUs ml −1 ) after plating pooled samples of 44 microtitre plate wells (75 μl sampling volume per well per time point) originating from 0.038 μg ml −1 rifampicin ( c ) and 0.38 μg ml −1 rifampicin ( d ) treatments (duplicate assays of the ones shown in a , b based on the same pre-culture) directly into 0, 0.38, and 3.8 μg ml −1 RIF, respectively; average and s.e.m. of duplicates are shown. ( e ) Sequencing of rpoB of 12 isolates originating from spontaneously resistant populations during 0.038-μg ml −1 RIF treatment, with the relevant RpoB amino-acid sequence (bold) together with the codons inside the dashed frame and their position in italics above; below, point mutations, the increase in resistance they generate and their frequency of occurrence are indicated.

    Article Snippet: Microtitre plate reader experiments were performed using a TECAN infinite pro 200 plate reader (Tecan Group) by measuring every 10 min with the following protocol: 5 s shaking, OD (595 nm) measurement with 25 flashes, luminescence measurement with an integration time of 1 s. Plate reader assays proved to represent a straightforward method for analysing growth and gene expression profiles of pneumococci in a high-throughput manner.

    Techniques: Mutagenesis, Expressing, Sampling, Sequencing

    Se CobB L , Se CobB S , and Ea CobB L proteins restore growth of an S. enterica cobB mutant strain on low acetate. (A) Growth behavior of S. enterica on MOPS minimal medium supplemented with acetate (10 mM). Growth experiments were performed at 37°C using a microtiter plate and a microtiter plate reader (Bio-Tek Instruments). (B) CobB Western blot analysis of S. enterica cobB strains overproducing Se CobB L , Se CobB S , or CobB L/S during growth on acetate (10 mM). Se CobB L is CobB M37A M38A , encoded by cobB1372 ; Se CobB S is CobB M1A , encoded by cobB1373 .

    Journal: Journal of Bacteriology

    Article Title: Biologically Active Isoforms of CobB Sirtuin Deacetylase in Salmonella enterica and Erwinia amylovora ▿ ▿ †

    doi: 10.1128/JB.00874-10

    Figure Lengend Snippet: Se CobB L , Se CobB S , and Ea CobB L proteins restore growth of an S. enterica cobB mutant strain on low acetate. (A) Growth behavior of S. enterica on MOPS minimal medium supplemented with acetate (10 mM). Growth experiments were performed at 37°C using a microtiter plate and a microtiter plate reader (Bio-Tek Instruments). (B) CobB Western blot analysis of S. enterica cobB strains overproducing Se CobB L , Se CobB S , or CobB L/S during growth on acetate (10 mM). Se CobB L is CobB M37A M38A , encoded by cobB1372 ; Se CobB S is CobB M1A , encoded by cobB1373 .

    Article Snippet: For growth curves, cell density was measured using a microtiter plate and a microtiter plate reader (Bio-Tek Instruments).

    Techniques: Mutagenesis, Western Blot

    Biofilm formation and EPS production by T. thermophilus HB27 strains. A , biofilm formation by strains of T. thermophilus HB27 overexpressing various PilF proteins. Biofilms formed on 96-well microtiter plates after 24 h were stained with crystal violet.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Phosphoproteomic Analysis Reveals the Effects of PilF Phosphorylation on Type IV Pilus and Biofilm Formation in Thermus thermophilus HB27 *

    doi: 10.1074/mcp.M113.029330

    Figure Lengend Snippet: Biofilm formation and EPS production by T. thermophilus HB27 strains. A , biofilm formation by strains of T. thermophilus HB27 overexpressing various PilF proteins. Biofilms formed on 96-well microtiter plates after 24 h were stained with crystal violet.

    Article Snippet: A microtiter plate reader (MRX Revelation, Dynex Technologies, Denkendorf, Germany) was used to read the optical density at 595 nm.

    Techniques: Staining

    Monospecies cariogenic biofilm biomass is influenced by carbohydrate source. Biomass was quantified spectrophotometrically by reading absorbance at 570 nm in a microtiter plate reader (FluoStar Omega, BMG Labtech). Four replicates were used for each isolate and was carried out on triplicate separate occasions, with the mean of each represented. Data represents mean with significance ∗ p

    Journal: Frontiers in Microbiology

    Article Title: Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community

    doi: 10.3389/fmicb.2018.01498

    Figure Lengend Snippet: Monospecies cariogenic biofilm biomass is influenced by carbohydrate source. Biomass was quantified spectrophotometrically by reading absorbance at 570 nm in a microtiter plate reader (FluoStar Omega, BMG Labtech). Four replicates were used for each isolate and was carried out on triplicate separate occasions, with the mean of each represented. Data represents mean with significance ∗ p

    Article Snippet: Biomass was quantified spectrophotometrically by reading absorbance at 570 nm in a microtiter plate reader (FluoStar Omega, BMG Labtech).

    Techniques:

    Binding of CRP to immobilized proteins at acidic pH. Results of a protein ligand-binding assay are shown. Microtiter wells were coated with complement factor H, amyloid β, BSA, and gelatin. CRP (10 μg/ml), diluted in TBS-Ca (pH 7.0 to 5.0), was then added to the wells. Bound CRP was detected by using a rabbit anti-CRP antibody and HRP-conjugated donkey anti-rabbit IgG. The absorbance of the developed color was read at 405 nm. A representative of three experiments is shown.

    Journal: The Journal of Biological Chemistry

    Article Title: Exposing a Hidden Functional Site of C-reactive Protein by Site-directed Mutagenesis *

    doi: 10.1074/jbc.M111.310011

    Figure Lengend Snippet: Binding of CRP to immobilized proteins at acidic pH. Results of a protein ligand-binding assay are shown. Microtiter wells were coated with complement factor H, amyloid β, BSA, and gelatin. CRP (10 μg/ml), diluted in TBS-Ca (pH 7.0 to 5.0), was then added to the wells. Bound CRP was detected by using a rabbit anti-CRP antibody and HRP-conjugated donkey anti-rabbit IgG. The absorbance of the developed color was read at 405 nm. A representative of three experiments is shown.

    Article Snippet: Color was developed, and the absorbance was read at 405 nm in a microtiter plate reader.

    Techniques: Binding Assay, Ligand Binding Assay

    Antibacterial activity of antibiotics in combination with the Trp-containing peptides. Bacterial cultures were treated with a series of concentration of antibiotics in the presence of a low peptide concentration equivalent to one-fourth of its MIC at 37°C overnight. The OD600 was recorded using a microtiter plate reader. The MIC was defined as the lowest antibiotics concentration that inhibited 95% of the bacterial growth. Each data point represents an average of six independent experiments.

    Journal: Frontiers in Microbiology

    Article Title: Synergistic Antibacterial Activity of Designed Trp-Containing Antibacterial Peptides in Combination With Antibiotics Against Multidrug-Resistant Staphylococcus epidermidis

    doi: 10.3389/fmicb.2019.02719

    Figure Lengend Snippet: Antibacterial activity of antibiotics in combination with the Trp-containing peptides. Bacterial cultures were treated with a series of concentration of antibiotics in the presence of a low peptide concentration equivalent to one-fourth of its MIC at 37°C overnight. The OD600 was recorded using a microtiter plate reader. The MIC was defined as the lowest antibiotics concentration that inhibited 95% of the bacterial growth. Each data point represents an average of six independent experiments.

    Article Snippet: Biofilms were stained with 0.1% (w/v) crystal violet dye (CV) for 5 min and quantified by reading at 590 nm in a microtiter plate reader (Varioskan Flash Microplate Reader, Thermo Scientific Co., Beijing).

    Techniques: Activity Assay, Concentration Assay

    Cross-species reactivity of clec14a-CTLD IgGs to human and mouse CTLDs. ELISA was performed with purified, selected IgG scFv clones (clones 1–4) on 96-well microtiter plates coated with hCTLD-Fc (▪), mCTLD-Fc (□) and Fc (□). BSA ( ) served as a background control. Values represent mean±s.d. of triplicate measurements from one of two independent experiments.

    Journal: Oncogene

    Article Title: Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro

    doi: 10.1038/onc.2013.156

    Figure Lengend Snippet: Cross-species reactivity of clec14a-CTLD IgGs to human and mouse CTLDs. ELISA was performed with purified, selected IgG scFv clones (clones 1–4) on 96-well microtiter plates coated with hCTLD-Fc (▪), mCTLD-Fc (□) and Fc (□). BSA ( ) served as a background control. Values represent mean±s.d. of triplicate measurements from one of two independent experiments.

    Article Snippet: Optical density was measured at 450 nm using a microtiter plate reader.

    Techniques: Enzyme-linked Immunosorbent Assay, Purification, Clone Assay

    DNase I and Proteinase K inhibit nicotine-induced biofilm formation. The biofilm formation of S. aureus USA300 strain was detected using a microtiter plate assay by measuring crystal violet stained biofilm at OD570. DNase I (25 U/well) and Proteinase K (2 ug/ml) was added to the well in both 2 mg/ml nicotine treatment group and control group. ( A ) Treated by DNaseI, the average thickness of biofilms varied from 1.506 ± 0.04 to 0.450 ± 0.07 in the nicotine-treated group (n = 3) and from 0.720 ± 0.14 to 0.328 ± 0.05 in untreated group. (n = 3) ( B ) Proteinase K(2 ug/ml) disrupted biofilm formation, in the nicotine treated group decreasing from 1.300 ± 0.06 to 0.393 ± 0.02 (n = 3), and in the control group decreasing from 0.721 ± 0.14 to 0.316 ± 0.08 (n = 3). *P

    Journal: Scientific Reports

    Article Title: Effect of nicotine on Staphylococcus aureus biofilm formation and virulence factors

    doi: 10.1038/s41598-019-56627-0

    Figure Lengend Snippet: DNase I and Proteinase K inhibit nicotine-induced biofilm formation. The biofilm formation of S. aureus USA300 strain was detected using a microtiter plate assay by measuring crystal violet stained biofilm at OD570. DNase I (25 U/well) and Proteinase K (2 ug/ml) was added to the well in both 2 mg/ml nicotine treatment group and control group. ( A ) Treated by DNaseI, the average thickness of biofilms varied from 1.506 ± 0.04 to 0.450 ± 0.07 in the nicotine-treated group (n = 3) and from 0.720 ± 0.14 to 0.328 ± 0.05 in untreated group. (n = 3) ( B ) Proteinase K(2 ug/ml) disrupted biofilm formation, in the nicotine treated group decreasing from 1.300 ± 0.06 to 0.393 ± 0.02 (n = 3), and in the control group decreasing from 0.721 ± 0.14 to 0.316 ± 0.08 (n = 3). *P

    Article Snippet: Subsequently, 200 μL of 10% acetic acid was added to each well and incubated for 1 h. Finally, the stained biofilms were quantified by estimating the optical density (OD) at 570 nm using a microtiter-plate reader (DTX 880 Multimode Detector; Beckman Coulter, USA).

    Techniques: Staining

    In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp microtiter plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.

    Journal: Scientific Reports

    Article Title: Development of camelid single chain antibodies against Shiga toxin type 2 (Stx2) with therapeutic potential against Hemolytic Uremic Syndrome (HUS)

    doi: 10.1038/srep24913

    Figure Lengend Snippet: In vitro activity of 2vb27 VHH under different formats. ( A ) Serially diluted purified 2vb27, (2vb27) 2 or (2vb27) 2 -SA were tested on the Vero cell neutralization assay as detailed in Materials and Methods. Each sample was tested by quadruplicate and is represented as mean ± SEM. ( B ) Binding of (2vb27) 2 -SA to human seroalbumin (SA). Nickel coated Maxisorp microtiter plates were incubated with serially diluted (2vb27) 2 -SA. Biotinylated human SA was added and binding was detected with Streptavidin-HRP. Reaction was developed with TMB and absorbance was read at 450 nm.

    Article Snippet: Cells were washed with PBS, stained with crystal violet dye (0.1% crystal violet in 20% methanol:water solution) and read on a microtiter plate reader (Asys UVM340; Biochrom Ltd., Cambridge, England) with a 570 nm filter.

    Techniques: In Vitro, Activity Assay, Purification, Neutralization, Binding Assay, Incubation