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  • 99
    Thermo Fisher microscope slide
    Microscope Slide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscope slide/product/Thermo Fisher
    Average 99 stars, based on 1498 article reviews
    Price from $9.99 to $1999.99
    microscope slide - by Bioz Stars, 2020-05
    99/100 stars
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    99
    Millipore chambered microscope slides
    <t>CARS</t> images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) <t>TPF</t> xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.
    Chambered Microscope Slides, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chambered microscope slides/product/Millipore
    Average 99 stars, based on 746 article reviews
    Price from $9.99 to $1999.99
    chambered microscope slides - by Bioz Stars, 2020-05
    99/100 stars
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    93
    Millipore microscope slide
    <t>CARS</t> images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) <t>TPF</t> xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.
    Microscope Slide, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscope slide/product/Millipore
    Average 93 stars, based on 828 article reviews
    Price from $9.99 to $1999.99
    microscope slide - by Bioz Stars, 2020-05
    93/100 stars
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    86
    Carl Zeiss microscopy a slide scanning microscope
    <t>CARS</t> images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) <t>TPF</t> xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.
    Microscopy A Slide Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscopy a slide scanning microscope/product/Carl Zeiss
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microscopy a slide scanning microscope - by Bioz Stars, 2020-05
    86/100 stars
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    99
    Thermo Fisher frosted slides
    <t>CARS</t> images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) <t>TPF</t> xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.
    Frosted Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/frosted slides/product/Thermo Fisher
    Average 99 stars, based on 167 article reviews
    Price from $9.99 to $1999.99
    frosted slides - by Bioz Stars, 2020-05
    99/100 stars
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    99
    Thermo Fisher superfrost plus glass microscopy slides
    <t>CARS</t> images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) <t>TPF</t> xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.
    Superfrost Plus Glass Microscopy Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfrost plus glass microscopy slides/product/Thermo Fisher
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    superfrost plus glass microscopy slides - by Bioz Stars, 2020-05
    99/100 stars
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    Image Search Results


    CARS images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) TPF xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.

    Journal: Development (Cambridge, England)

    Article Title: Quantitative imaging of lipids in live mouse oocytes and early embryos using CARS microscopy

    doi: 10.1242/dev.129908

    Figure Lengend Snippet: CARS images compared with conventional fluorescent lipid dyes on fixed eggs. (A-D) DIC (using a 1.27 NA water objective and a 1.4 NA oil condenser), (E-H) false-coloured CARS images at wavenumber 2850 cm −1 and (I-L) TPF xy images, accompanied by (M-P) false-coloured overlays, of GV and MII eggs stained with BODIPY ( n =23 and 30, respectively) or LipidTox green neutral lipid stain ( n =7 and 20, respectively). All images are maximum intensity projections of stacks with 0.5 µm z -steps. 0.1×0.1 µm pixel size; 0.01 ms pixel dwell time; ∼12 mW (∼9 mW) pump (Stokes) power at the sample. CARS and TPF signal intensities are given in photoelectrons per second (ph.e − /s). Scale bars: 10 µm. (Q-T) Scatterplots of pixel-coordinate correlation between CARS ( x -axis) and TPF images ( y -axis), and the mean Pearson's correlation coefficients of all investigated eggs, to show the degree of reliability of these dyes. A colocalisation is apparent with a Pearson's coefficient of > 0.5, but is only considered significant if > 0.95 as in accordance with normal 95% confidence limits. Data from ≥4 trials, using 1-3 mice each.

    Article Snippet: CARS, DIC and TPF microscopy Standard microscope slides were prepared with SecureSeal imaging spacers (Sigma) of ∼150 μm thickness.

    Techniques: Staining, Mass Spectrometry, Mouse Assay