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  • 94
    Sutter Instrument micropipette puller
    Microneedle tip dimensions and pull parameters A Sutter <t>P-97</t> micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.
    Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 94/100, based on 3009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micropipette puller/product/Sutter Instrument
    Average 94 stars, based on 3009 article reviews
    Price from $9.99 to $1999.99
    micropipette puller - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    92
    Narishige micropipette puller
    Microneedle tip dimensions and pull parameters A Sutter <t>P-97</t> micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.
    Micropipette Puller, supplied by Narishige, used in various techniques. Bioz Stars score: 92/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micropipette puller/product/Narishige
    Average 92 stars, based on 456 article reviews
    Price from $9.99 to $1999.99
    micropipette puller - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    89
    Narishige pc 10 micropipette puller
    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige <t>PC-10</t> glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).
    Pc 10 Micropipette Puller, supplied by Narishige, used in various techniques. Bioz Stars score: 89/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc 10 micropipette puller/product/Narishige
    Average 89 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    pc 10 micropipette puller - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    88
    Sutter Instrument laser based micropipette puller
    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige <t>PC-10</t> glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).
    Laser Based Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 88/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laser based micropipette puller/product/Sutter Instrument
    Average 88 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    laser based micropipette puller - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    88
    Sutter Instrument p 80 micropipette puller
    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige <t>PC-10</t> glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).
    P 80 Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 88/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p 80 micropipette puller/product/Sutter Instrument
    Average 88 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    p 80 micropipette puller - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Microneedle tip dimensions and pull parameters A Sutter P-97 micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling

    doi: 10.1007/978-1-4939-3530-7_16

    Figure Lengend Snippet: Microneedle tip dimensions and pull parameters A Sutter P-97 micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.

    Article Snippet: 7 Using a P-97 micropipette puller (Sutter), we had success with the following pull parameters: HEAT: 513, PULL: 250, VEL: 220, TIME: 200.

    Techniques:

    Needle preparation. (A) Needles were pulled using a Sutter P-2000 micropipette puller. (B) (C) Pulled needles were beveled to an angle of 20° using a Sutter BV-10 Microelectrode Beveller, 104D fine abrasive plate. (D) Needles were inspected under a microscope to ensure the bore is not greater than 1 μm. NOTE: If there is no availability of a needle beveller, needles can be opened by breaking the bore towards a glass slide under the microscope (always verify the opening is smaller than 1 μm).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9

    doi: 10.1371/journal.pntd.0006769

    Figure Lengend Snippet: Needle preparation. (A) Needles were pulled using a Sutter P-2000 micropipette puller. (B) (C) Pulled needles were beveled to an angle of 20° using a Sutter BV-10 Microelectrode Beveller, 104D fine abrasive plate. (D) Needles were inspected under a microscope to ensure the bore is not greater than 1 μm. NOTE: If there is no availability of a needle beveller, needles can be opened by breaking the bore towards a glass slide under the microscope (always verify the opening is smaller than 1 μm).

    Article Snippet: Pulling needles Borosilicate glass capillaries needles (#1B100-4, Kwik-Fil) were laser-pulled using a Sutter P-2000 micropipette puller (Novato, CA, USA, ) and parameters were set as follows: Heat = 270, FIL = 3, VEL = 25, DEL = 250, PUL = 140.

    Techniques: Microscopy

    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige PC-10 glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).

    Journal: Star Protocols

    Article Title: In Utero Cortical Electroporation of Plasmids in the Mouse Embryo

    doi: 10.1016/j.xpro.2020.100027

    Figure Lengend Snippet: Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige PC-10 glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).

    Article Snippet: We use borosilicated microcapillary and prepare them with a Narishige PC-10 micropipette puller.

    Techniques: Plasmid Preparation, Injection, Transferring