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    • micropipette puller  (Sutter Instrument)
    94
    Sutter Instrument micropipette puller
    Microneedle tip dimensions and pull parameters A Sutter <t>P-97</t> micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.
    Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 94/100, based on 3009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micropipette puller/product/Sutter Instrument
    Average 94 stars, based on 3009 article reviews
    Price from $9.99 to $1999.99
    micropipette puller - by Bioz Stars, 2020-09
    94/100 stars
    		  Buy from Supplier
    micropipette puller  (Narishige)
    92
    Narishige micropipette puller
    Microneedle tip dimensions and pull parameters A Sutter <t>P-97</t> micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.
    Micropipette Puller, supplied by Narishige, used in various techniques. Bioz Stars score: 92/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micropipette puller/product/Narishige
    Average 92 stars, based on 456 article reviews
    Price from $9.99 to $1999.99
    micropipette puller - by Bioz Stars, 2020-09
    92/100 stars
    		  Buy from Supplier
    pc 10 micropipette puller  (Narishige)
    89
    Narishige pc 10 micropipette puller
    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige <t>PC-10</t> glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).
    Pc 10 Micropipette Puller, supplied by Narishige, used in various techniques. Bioz Stars score: 89/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc 10 micropipette puller/product/Narishige
    Average 89 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    pc 10 micropipette puller - by Bioz Stars, 2020-09
    89/100 stars
    		  Buy from Supplier
    laser based micropipette puller  (Sutter Instrument)
    88
    Sutter Instrument laser based micropipette puller
    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige <t>PC-10</t> glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).
    Laser Based Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 88/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laser based micropipette puller/product/Sutter Instrument
    Average 88 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    laser based micropipette puller - by Bioz Stars, 2020-09
    88/100 stars
    		  Buy from Supplier
    p 80 micropipette puller  (Sutter Instrument)
    88
    Sutter Instrument p 80 micropipette puller
    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige <t>PC-10</t> glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).
    P 80 Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 88/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p 80 micropipette puller/product/Sutter Instrument
    Average 88 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    p 80 micropipette puller - by Bioz Stars, 2020-09
    88/100 stars
    		  Buy from Supplier

    Image Search Results


    Microneedle tip dimensions and pull parameters A Sutter P-97 micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling

    doi: 10.1007/978-1-4939-3530-7_16

    Figure Lengend Snippet: Microneedle tip dimensions and pull parameters A Sutter P-97 micropipette puller was used to create microharpoons from borosilicate glass rods. A tip diameter of ≈1 μm (left) or smaller is well suited for the microharpoon assay. Different tip diameters and taper lengths result from changing the pull parameters (right, lower HEAT and PULL). Note that a wider tip (right) is less ideal for the microharpoon assay as it could damage the cell membrane. Scale bar: 10 μm.

    Article Snippet: 7 Using a P-97 micropipette puller (Sutter), we had success with the following pull parameters: HEAT: 513, PULL: 250, VEL: 220, TIME: 200.

    Techniques:

    Needle preparation. (A) Needles were pulled using a Sutter P-2000 micropipette puller. (B) (C) Pulled needles were beveled to an angle of 20° using a Sutter BV-10 Microelectrode Beveller, 104D fine abrasive plate. (D) Needles were inspected under a microscope to ensure the bore is not greater than 1 μm. NOTE: If there is no availability of a needle beveller, needles can be opened by breaking the bore towards a glass slide under the microscope (always verify the opening is smaller than 1 μm).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9

    doi: 10.1371/journal.pntd.0006769

    Figure Lengend Snippet: Needle preparation. (A) Needles were pulled using a Sutter P-2000 micropipette puller. (B) (C) Pulled needles were beveled to an angle of 20° using a Sutter BV-10 Microelectrode Beveller, 104D fine abrasive plate. (D) Needles were inspected under a microscope to ensure the bore is not greater than 1 μm. NOTE: If there is no availability of a needle beveller, needles can be opened by breaking the bore towards a glass slide under the microscope (always verify the opening is smaller than 1 μm).

    Article Snippet: Pulling needles Borosilicate glass capillaries needles (#1B100-4, Kwik-Fil) were laser-pulled using a Sutter P-2000 micropipette puller (Novato, CA, USA, ) and parameters were set as follows: Heat = 270, FIL = 3, VEL = 25, DEL = 250, PUL = 140.

    Techniques: Microscopy

    Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige PC-10 glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).

    Journal: Star Protocols

    Article Title: In Utero Cortical Electroporation of Plasmids in the Mouse Embryo

    doi: 10.1016/j.xpro.2020.100027

    Figure Lengend Snippet: Preparation of Microcapillaries for Plasmid Injection (A and B) (A) Use of the Narishige PC-10 glass pipette puller. Result can be seen in (B): unbroken pipette after pulling (a.). Pipette tip can be broken with a gentle finger push on the glass tip. Pipette break must be clean and narrow to ensure appropriate plasmid injection with minimum damage to brain structures (b.). In some instances a broader aperture might be better suited when using more viscous DNA solutions (c.). Too wide and/or not sharp breakage should be avoided for they induce too much brain damage (d.). Tip of a 27 gauge needle for scale (e.).

    Article Snippet: We use borosilicated microcapillary and prepare them with a Narishige PC-10 micropipette puller.

    Techniques: Plasmid Preparation, Injection, Transferring