microgel filtration Search Results


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  • 96
    ATCC e coli atcc 43888
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    E Coli Atcc 43888, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96/100 stars
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    99
    Thermo Fisher microtitre plate
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Microtitre Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SCP SCIENCE teflon filtration system
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Teflon Filtration System, supplied by SCP SCIENCE, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore vacuum driven filtration
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Vacuum Driven Filtration, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore 96 well multiscreen dv filtration plate
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    96 Well Multiscreen Dv Filtration Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Beckman Coulter microcells
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Microcells, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Corning Life Sciences microwell plates
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Microwell Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher nunc 96 well polypropylene microwell plates
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Nunc 96 Well Polypropylene Microwell Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher 96 well microwell round bottom plates
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    96 Well Microwell Round Bottom Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Perry Laboratory peroxidase conjugated anti mouse immunoglobulin g tmb microwell peroxidase substrate
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Peroxidase Conjugated Anti Mouse Immunoglobulin G Tmb Microwell Peroxidase Substrate, supplied by Perry Laboratory, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare superdex s200 pc
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Superdex S200 Pc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore membrane filter
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Membrane Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies chromatography analysis
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Chromatography Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher nalgene polysulfone reusable bottle top filters
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Nalgene Polysulfone Reusable Bottle Top Filters, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    SPECTRO Analytical plasma emission spectrometer
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Plasma Emission Spectrometer, supplied by SPECTRO Analytical, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche rs lymphoblastoid cells
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Rs Lymphoblastoid Cells, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare sterile whatman paper
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Sterile Whatman Paper, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher protein a sepharose 4b
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Protein A Sepharose 4b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies 1200 hplc system
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    1200 Hplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 5707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore levofloxacin
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 <t>ATCC</t> 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    Levofloxacin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dhe
    Oligomerization of <t>NPC2</t> and in vitro loading and unloading of lipids into and from CD1d by recombinant NPC2. (A) Gel filtration profile and Coomassie blue gel of purified recombinant NPC2 protein. Fractions containing monomers and enriched in NPC2 dimers are indicated (arrows in the profile; monomers [m] and dimers [d] in the gel). (B) Expression of NPC2 in HeLa cells. Lysates from transfected HeLa cells were boiled in the presence of SDS or left untreated and analyzed by Western blotting using anti-Flag antibody. (C) Interaction of fly-expressed NPC2 protein with <t>DHE.</t> Fluorescent spectra of samples containing 10 μM NPC2 monomer (NPC2m) and 10 μM NPC2 dimer (NPC2d) with or without 1 μM DHE. Spectra of samples containing buffer or 1 μM DHE only are also shown. (D) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of GM3 in the absence (white bars) or presence of 50 μM NPC2 dimers (gray bars) or 50 μM NPC2 monomers (black bars). The percentage of GM3 loading is indicated. (E) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 monomers (white bars) and dimers (black bars). Unloading is indicated as a percentage of GT 1b removal. (F) NPC2 dimers load iGb3 into CD1d. 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 dimers in the absence or presence of 20 μM iGb3 C 18 (iGb3). Lipid unloading and loading were visualized by native IEF. Relative positions of CD1d, CD1d–GT1b, and CD1d–iGb3 are indicated by arrows. Error bars represent SD.
    Dhe, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Waters Corporation water based gel permeation chromatography gpc system
    Oligomerization of <t>NPC2</t> and in vitro loading and unloading of lipids into and from CD1d by recombinant NPC2. (A) Gel filtration profile and Coomassie blue gel of purified recombinant NPC2 protein. Fractions containing monomers and enriched in NPC2 dimers are indicated (arrows in the profile; monomers [m] and dimers [d] in the gel). (B) Expression of NPC2 in HeLa cells. Lysates from transfected HeLa cells were boiled in the presence of SDS or left untreated and analyzed by Western blotting using anti-Flag antibody. (C) Interaction of fly-expressed NPC2 protein with <t>DHE.</t> Fluorescent spectra of samples containing 10 μM NPC2 monomer (NPC2m) and 10 μM NPC2 dimer (NPC2d) with or without 1 μM DHE. Spectra of samples containing buffer or 1 μM DHE only are also shown. (D) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of GM3 in the absence (white bars) or presence of 50 μM NPC2 dimers (gray bars) or 50 μM NPC2 monomers (black bars). The percentage of GM3 loading is indicated. (E) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 monomers (white bars) and dimers (black bars). Unloading is indicated as a percentage of GT 1b removal. (F) NPC2 dimers load iGb3 into CD1d. 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 dimers in the absence or presence of 20 μM iGb3 C 18 (iGb3). Lipid unloading and loading were visualized by native IEF. Relative positions of CD1d, CD1d–GT1b, and CD1d–iGb3 are indicated by arrows. Error bars represent SD.
    Water Based Gel Permeation Chromatography Gpc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/water based gel permeation chromatography gpc system/product/Waters Corporation
    Average 93 stars, based on 4 article reviews
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    99
    Millipore mixed cellulose ester membrane
    Oligomerization of <t>NPC2</t> and in vitro loading and unloading of lipids into and from CD1d by recombinant NPC2. (A) Gel filtration profile and Coomassie blue gel of purified recombinant NPC2 protein. Fractions containing monomers and enriched in NPC2 dimers are indicated (arrows in the profile; monomers [m] and dimers [d] in the gel). (B) Expression of NPC2 in HeLa cells. Lysates from transfected HeLa cells were boiled in the presence of SDS or left untreated and analyzed by Western blotting using anti-Flag antibody. (C) Interaction of fly-expressed NPC2 protein with <t>DHE.</t> Fluorescent spectra of samples containing 10 μM NPC2 monomer (NPC2m) and 10 μM NPC2 dimer (NPC2d) with or without 1 μM DHE. Spectra of samples containing buffer or 1 μM DHE only are also shown. (D) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of GM3 in the absence (white bars) or presence of 50 μM NPC2 dimers (gray bars) or 50 μM NPC2 monomers (black bars). The percentage of GM3 loading is indicated. (E) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 monomers (white bars) and dimers (black bars). Unloading is indicated as a percentage of GT 1b removal. (F) NPC2 dimers load iGb3 into CD1d. 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 dimers in the absence or presence of 20 μM iGb3 C 18 (iGb3). Lipid unloading and loading were visualized by native IEF. Relative positions of CD1d, CD1d–GT1b, and CD1d–iGb3 are indicated by arrows. Error bars represent SD.
    Mixed Cellulose Ester Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polysorbate 80
    Oligomerization of <t>NPC2</t> and in vitro loading and unloading of lipids into and from CD1d by recombinant NPC2. (A) Gel filtration profile and Coomassie blue gel of purified recombinant NPC2 protein. Fractions containing monomers and enriched in NPC2 dimers are indicated (arrows in the profile; monomers [m] and dimers [d] in the gel). (B) Expression of NPC2 in HeLa cells. Lysates from transfected HeLa cells were boiled in the presence of SDS or left untreated and analyzed by Western blotting using anti-Flag antibody. (C) Interaction of fly-expressed NPC2 protein with <t>DHE.</t> Fluorescent spectra of samples containing 10 μM NPC2 monomer (NPC2m) and 10 μM NPC2 dimer (NPC2d) with or without 1 μM DHE. Spectra of samples containing buffer or 1 μM DHE only are also shown. (D) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of GM3 in the absence (white bars) or presence of 50 μM NPC2 dimers (gray bars) or 50 μM NPC2 monomers (black bars). The percentage of GM3 loading is indicated. (E) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 monomers (white bars) and dimers (black bars). Unloading is indicated as a percentage of GT 1b removal. (F) NPC2 dimers load iGb3 into CD1d. 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 dimers in the absence or presence of 20 μM iGb3 C 18 (iGb3). Lipid unloading and loading were visualized by native IEF. Relative positions of CD1d, CD1d–GT1b, and CD1d–iGb3 are indicated by arrows. Error bars represent SD.
    Polysorbate 80, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 ATCC 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.

    Journal: Applied and Environmental Microbiology

    Article Title: Abundance in Sewage of Bacteriophages That Infect Escherichia coli O157:H7 and That Carry the Shiga Toxin 2 Gene

    doi:

    Figure Lengend Snippet: (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 ATCC 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.

    Article Snippet: Supernatants of cultures of E. coli ATCC 43888 were tested to exclude their potential effect on Vero cells.

    Techniques: Western Blot, Infection, Derivative Assay, Stripping Membranes, SDS Page, Filtration, Centrifugation

    Visual estimation of Vero cell cytotoxicity. Wells 1 to 5 were inoculated with growth medium; well 6 was inoculated with sewage that had been centrifuged, filtered, and DNase treated; wells 7, 8, 9, and 10 were inoculated with 10-fold dilutions of a crude supernatant of E. coli O157:H7 ATCC 43888 infected with 10 ml of sewage that had been centrifuged, filtered, and DNase treated. Wells 11, 12, 13, 14, and 15 were like wells 6, 7, 8, 9, and 10, respectively, but with the addition of anti-Stx 2 subunit A MAb 11E10.

    Journal: Applied and Environmental Microbiology

    Article Title: Abundance in Sewage of Bacteriophages That Infect Escherichia coli O157:H7 and That Carry the Shiga Toxin 2 Gene

    doi:

    Figure Lengend Snippet: Visual estimation of Vero cell cytotoxicity. Wells 1 to 5 were inoculated with growth medium; well 6 was inoculated with sewage that had been centrifuged, filtered, and DNase treated; wells 7, 8, 9, and 10 were inoculated with 10-fold dilutions of a crude supernatant of E. coli O157:H7 ATCC 43888 infected with 10 ml of sewage that had been centrifuged, filtered, and DNase treated. Wells 11, 12, 13, 14, and 15 were like wells 6, 7, 8, 9, and 10, respectively, but with the addition of anti-Stx 2 subunit A MAb 11E10.

    Article Snippet: Supernatants of cultures of E. coli ATCC 43888 were tested to exclude their potential effect on Vero cells.

    Techniques: Infection

    Oligomerization of NPC2 and in vitro loading and unloading of lipids into and from CD1d by recombinant NPC2. (A) Gel filtration profile and Coomassie blue gel of purified recombinant NPC2 protein. Fractions containing monomers and enriched in NPC2 dimers are indicated (arrows in the profile; monomers [m] and dimers [d] in the gel). (B) Expression of NPC2 in HeLa cells. Lysates from transfected HeLa cells were boiled in the presence of SDS or left untreated and analyzed by Western blotting using anti-Flag antibody. (C) Interaction of fly-expressed NPC2 protein with DHE. Fluorescent spectra of samples containing 10 μM NPC2 monomer (NPC2m) and 10 μM NPC2 dimer (NPC2d) with or without 1 μM DHE. Spectra of samples containing buffer or 1 μM DHE only are also shown. (D) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of GM3 in the absence (white bars) or presence of 50 μM NPC2 dimers (gray bars) or 50 μM NPC2 monomers (black bars). The percentage of GM3 loading is indicated. (E) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 monomers (white bars) and dimers (black bars). Unloading is indicated as a percentage of GT 1b removal. (F) NPC2 dimers load iGb3 into CD1d. 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 dimers in the absence or presence of 20 μM iGb3 C 18 (iGb3). Lipid unloading and loading were visualized by native IEF. Relative positions of CD1d, CD1d–GT1b, and CD1d–iGb3 are indicated by arrows. Error bars represent SD.

    Journal: The Journal of Experimental Medicine

    Article Title: The Niemann-Pick type C2 protein loads isoglobotrihexosylceramide onto CD1d molecules and contributes to the thymic selection of NKT cells

    doi: 10.1084/jem.20061562

    Figure Lengend Snippet: Oligomerization of NPC2 and in vitro loading and unloading of lipids into and from CD1d by recombinant NPC2. (A) Gel filtration profile and Coomassie blue gel of purified recombinant NPC2 protein. Fractions containing monomers and enriched in NPC2 dimers are indicated (arrows in the profile; monomers [m] and dimers [d] in the gel). (B) Expression of NPC2 in HeLa cells. Lysates from transfected HeLa cells were boiled in the presence of SDS or left untreated and analyzed by Western blotting using anti-Flag antibody. (C) Interaction of fly-expressed NPC2 protein with DHE. Fluorescent spectra of samples containing 10 μM NPC2 monomer (NPC2m) and 10 μM NPC2 dimer (NPC2d) with or without 1 μM DHE. Spectra of samples containing buffer or 1 μM DHE only are also shown. (D) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of GM3 in the absence (white bars) or presence of 50 μM NPC2 dimers (gray bars) or 50 μM NPC2 monomers (black bars). The percentage of GM3 loading is indicated. (E) 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 monomers (white bars) and dimers (black bars). Unloading is indicated as a percentage of GT 1b removal. (F) NPC2 dimers load iGb3 into CD1d. 2.5 μM CD1d–GT 1b complexes were incubated with increasing concentrations of NPC2 dimers in the absence or presence of 20 μM iGb3 C 18 (iGb3). Lipid unloading and loading were visualized by native IEF. Relative positions of CD1d, CD1d–GT1b, and CD1d–iGb3 are indicated by arrows. Error bars represent SD.

    Article Snippet: For binding measurements, NPC2 and DHE (Sigma-Aldrich) were diluted in PBS and incubated for 30 min at 25°C in the dark.

    Techniques: In Vitro, Recombinant, Filtration, Purification, Expressing, Transfection, Western Blot, Incubation, Electrofocusing