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  • 94
    New England Biolabs monarch microfuge tube ecorack
    Monarch Microfuge Tube Ecorack, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch microfuge tube ecorack/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    99
    Thermo Fisher microfuge tube
    Microfuge Tube, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfuge tube/product/Thermo Fisher
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    92
    Fisher Scientific microfuge tube
    Microfuge Tube, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfuge tube/product/Fisher Scientific
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    92
    Axygen microfuge tube
    AT-associated PET metabolism in the Ce as a rationale for laser capture microdissection and sequencing of CeL neurons. (A) Using data from our large sample of AT-phenotyped and brain imaged animals, we previously demonstrated that individual differences in NEC-associated PET metabolism are related to individual differences in AT (yellow, ( Oler et al., 2010 ). We identified the peak AT voxels (pink) to be in the Ce region by demonstrating an overlap with serotonin transporter binding (blue), which, relative to surrounding regions, is elevated in the CeL. (B) Brain slabs containing the amygdala were identified and the A-P location was determined before sectioning. Tissue was sectioned and mounted on LCM slides (orange and blue labels). Adjacent slides were stained with AChE (green labels) to determine the location of the CeL. LCM slides were stained with an abbreviated NeuN protocol and CeL neurons were captured into the lid of a <t>microfuge</t> tube. Post-cut overlays were made for every slide to confirm capture location. RNA from 500-600 CeL neurons was pooled and used for RNA-Seq (n=47). Reads were processed and aligned to MacaM ( Zimin et al., 2014 ).
    Microfuge Tube, supplied by Axygen, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfuge tube/product/Axygen
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    90
    Corning Life Sciences microfuge tubes
    AT-associated PET metabolism in the Ce as a rationale for laser capture microdissection and sequencing of CeL neurons. (A) Using data from our large sample of AT-phenotyped and brain imaged animals, we previously demonstrated that individual differences in NEC-associated PET metabolism are related to individual differences in AT (yellow, ( Oler et al., 2010 ). We identified the peak AT voxels (pink) to be in the Ce region by demonstrating an overlap with serotonin transporter binding (blue), which, relative to surrounding regions, is elevated in the CeL. (B) Brain slabs containing the amygdala were identified and the A-P location was determined before sectioning. Tissue was sectioned and mounted on LCM slides (orange and blue labels). Adjacent slides were stained with AChE (green labels) to determine the location of the CeL. LCM slides were stained with an abbreviated NeuN protocol and CeL neurons were captured into the lid of a <t>microfuge</t> tube. Post-cut overlays were made for every slide to confirm capture location. RNA from 500-600 CeL neurons was pooled and used for RNA-Seq (n=47). Reads were processed and aligned to MacaM ( Zimin et al., 2014 ).
    Microfuge Tubes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Greiner Bio microfuge tubes
    AT-associated PET metabolism in the Ce as a rationale for laser capture microdissection and sequencing of CeL neurons. (A) Using data from our large sample of AT-phenotyped and brain imaged animals, we previously demonstrated that individual differences in NEC-associated PET metabolism are related to individual differences in AT (yellow, ( Oler et al., 2010 ). We identified the peak AT voxels (pink) to be in the Ce region by demonstrating an overlap with serotonin transporter binding (blue), which, relative to surrounding regions, is elevated in the CeL. (B) Brain slabs containing the amygdala were identified and the A-P location was determined before sectioning. Tissue was sectioned and mounted on LCM slides (orange and blue labels). Adjacent slides were stained with AChE (green labels) to determine the location of the CeL. LCM slides were stained with an abbreviated NeuN protocol and CeL neurons were captured into the lid of a <t>microfuge</t> tube. Post-cut overlays were made for every slide to confirm capture location. RNA from 500-600 CeL neurons was pooled and used for RNA-Seq (n=47). Reads were processed and aligned to MacaM ( Zimin et al., 2014 ).
    Microfuge Tubes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    USA Scientific Inc microfuge tubes
    AT-associated PET metabolism in the Ce as a rationale for laser capture microdissection and sequencing of CeL neurons. (A) Using data from our large sample of AT-phenotyped and brain imaged animals, we previously demonstrated that individual differences in NEC-associated PET metabolism are related to individual differences in AT (yellow, ( Oler et al., 2010 ). We identified the peak AT voxels (pink) to be in the Ce region by demonstrating an overlap with serotonin transporter binding (blue), which, relative to surrounding regions, is elevated in the CeL. (B) Brain slabs containing the amygdala were identified and the A-P location was determined before sectioning. Tissue was sectioned and mounted on LCM slides (orange and blue labels). Adjacent slides were stained with AChE (green labels) to determine the location of the CeL. LCM slides were stained with an abbreviated NeuN protocol and CeL neurons were captured into the lid of a <t>microfuge</t> tube. Post-cut overlays were made for every slide to confirm capture location. RNA from 500-600 CeL neurons was pooled and used for RNA-Seq (n=47). Reads were processed and aligned to MacaM ( Zimin et al., 2014 ).
    Microfuge Tubes, supplied by USA Scientific Inc, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor microfuge tubes
    Schematic representation of the in situ PCR pipeline. Plant tissue is fixed in an ethanol/acetic acid/formaldehyde solution, followed by embedding in agarose and sectioning. Sections are collected into a <t>microfuge</t> tube in which DNase treatment, reverse transcription and in situ PCR are carried out using a thermocycler. During in situ PCR, DIG labeled nucleotides are incorporated into the PCR products. An anti-DIG antibody conjugated with alkaline phosphatase and an alkaline phosphatase substrate are used for the detection of the DIG labeled PCR products. These are visualized under a microscope. Thin fragile sections are placed onto a glass slide after sectioning, while *non-embedded, non-sectioned samples (i.e. epidermal peels) are placed directly onto slides for all processes from fixation to the final PCR step (dotted arrow).
    Microfuge Tubes, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biomatrica microfuge tubes
    Schematic representation of the in situ PCR pipeline. Plant tissue is fixed in an ethanol/acetic acid/formaldehyde solution, followed by embedding in agarose and sectioning. Sections are collected into a <t>microfuge</t> tube in which DNase treatment, reverse transcription and in situ PCR are carried out using a thermocycler. During in situ PCR, DIG labeled nucleotides are incorporated into the PCR products. An anti-DIG antibody conjugated with alkaline phosphatase and an alkaline phosphatase substrate are used for the detection of the DIG labeled PCR products. These are visualized under a microscope. Thin fragile sections are placed onto a glass slide after sectioning, while *non-embedded, non-sectioned samples (i.e. epidermal peels) are placed directly onto slides for all processes from fixation to the final PCR step (dotted arrow).
    Microfuge Tubes, supplied by Biomatrica, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beckman Coulter microfuge tubes
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Microfuge Tubes, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sarstedt microfuge tubes
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Microfuge Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad microfuge tube
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Microfuge Tube, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfuge tube/product/Bio-Rad
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    99
    Millipore microfuge tube
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Microfuge Tube, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    USA Scientific Inc copolymer microfuge tubes
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Copolymer Microfuge Tubes, supplied by USA Scientific Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore microfuge tubes
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Microfuge Tubes, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    New England Biolabs microfuge tube
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Microfuge Tube, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Beckman Coulter polyallomer microfuge tube
    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a <t>microfuge</t> and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .
    Polyallomer Microfuge Tube, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    AT-associated PET metabolism in the Ce as a rationale for laser capture microdissection and sequencing of CeL neurons. (A) Using data from our large sample of AT-phenotyped and brain imaged animals, we previously demonstrated that individual differences in NEC-associated PET metabolism are related to individual differences in AT (yellow, ( Oler et al., 2010 ). We identified the peak AT voxels (pink) to be in the Ce region by demonstrating an overlap with serotonin transporter binding (blue), which, relative to surrounding regions, is elevated in the CeL. (B) Brain slabs containing the amygdala were identified and the A-P location was determined before sectioning. Tissue was sectioned and mounted on LCM slides (orange and blue labels). Adjacent slides were stained with AChE (green labels) to determine the location of the CeL. LCM slides were stained with an abbreviated NeuN protocol and CeL neurons were captured into the lid of a microfuge tube. Post-cut overlays were made for every slide to confirm capture location. RNA from 500-600 CeL neurons was pooled and used for RNA-Seq (n=47). Reads were processed and aligned to MacaM ( Zimin et al., 2014 ).

    Journal: bioRxiv

    Article Title: Transcriptional Profiling of Primate Central Nucleus of the Amygdala Neurons to Understand the Molecular Underpinnings of Early Life Anxious Temperament

    doi: 10.1101/808279

    Figure Lengend Snippet: AT-associated PET metabolism in the Ce as a rationale for laser capture microdissection and sequencing of CeL neurons. (A) Using data from our large sample of AT-phenotyped and brain imaged animals, we previously demonstrated that individual differences in NEC-associated PET metabolism are related to individual differences in AT (yellow, ( Oler et al., 2010 ). We identified the peak AT voxels (pink) to be in the Ce region by demonstrating an overlap with serotonin transporter binding (blue), which, relative to surrounding regions, is elevated in the CeL. (B) Brain slabs containing the amygdala were identified and the A-P location was determined before sectioning. Tissue was sectioned and mounted on LCM slides (orange and blue labels). Adjacent slides were stained with AChE (green labels) to determine the location of the CeL. LCM slides were stained with an abbreviated NeuN protocol and CeL neurons were captured into the lid of a microfuge tube. Post-cut overlays were made for every slide to confirm capture location. RNA from 500-600 CeL neurons was pooled and used for RNA-Seq (n=47). Reads were processed and aligned to MacaM ( Zimin et al., 2014 ).

    Article Snippet: Neurons were identified at 20x magnification, dissected with the laser, and fell into the lid of a microfuge tube (PCR-05-C, Axygen, Corning, NY) that was filled with 40uL of lysis buffer.

    Techniques: Positron Emission Tomography, Laser Capture Microdissection, Sequencing, Binding Assay, Staining, RNA Sequencing Assay

    Schematic representation of the in situ PCR pipeline. Plant tissue is fixed in an ethanol/acetic acid/formaldehyde solution, followed by embedding in agarose and sectioning. Sections are collected into a microfuge tube in which DNase treatment, reverse transcription and in situ PCR are carried out using a thermocycler. During in situ PCR, DIG labeled nucleotides are incorporated into the PCR products. An anti-DIG antibody conjugated with alkaline phosphatase and an alkaline phosphatase substrate are used for the detection of the DIG labeled PCR products. These are visualized under a microscope. Thin fragile sections are placed onto a glass slide after sectioning, while *non-embedded, non-sectioned samples (i.e. epidermal peels) are placed directly onto slides for all processes from fixation to the final PCR step (dotted arrow).

    Journal: Plant Methods

    Article Title: Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue

    doi: 10.1186/1746-4811-10-29

    Figure Lengend Snippet: Schematic representation of the in situ PCR pipeline. Plant tissue is fixed in an ethanol/acetic acid/formaldehyde solution, followed by embedding in agarose and sectioning. Sections are collected into a microfuge tube in which DNase treatment, reverse transcription and in situ PCR are carried out using a thermocycler. During in situ PCR, DIG labeled nucleotides are incorporated into the PCR products. An anti-DIG antibody conjugated with alkaline phosphatase and an alkaline phosphatase substrate are used for the detection of the DIG labeled PCR products. These are visualized under a microscope. Thin fragile sections are placed onto a glass slide after sectioning, while *non-embedded, non-sectioned samples (i.e. epidermal peels) are placed directly onto slides for all processes from fixation to the final PCR step (dotted arrow).

    Article Snippet: •2 mL microfuge tubes (VWR, cat. no. 211–2120).

    Techniques: In Situ, Polymerase Chain Reaction, Labeling, Microscopy

    Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a microfuge and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .

    Journal: The Journal of Cell Biology

    Article Title: Mutations in the Middle of the Transmembrane Domain Reverse the Polarity of Transport of the Influenza Virus Hemagglutinin in MDCK Epithelial Cells

    doi:

    Figure Lengend Snippet: Mutations in the HA TM affect transport to the apical cell surface and solubility in Triton X-100. ( A ) MDCK monolayers expressing the HA types shown were grown on filter culture inserts for 5 d and then subjected to a pulse-chase protocol in which trypsin was present during the chase in either the apical ( Ap ) or basolateral ( Bl ) compartment. After the chase, HAs were immunoprecipitated, separated by PAGE, and then analyzed by a PhosphorImager. A representative image comparing HA and two mutants is shown. ( B ) MDCK cells expressing the mutants shown were pulse-labeled and chased for 40 min, then the cells were lysed in 1% Triton X-100 on ice. The cell lysate was centrifuged in a microfuge and separated into supernatant ( S ) and pellet ( P ) fractions. HA was immunoprecipitated from each fraction and analyzed as in A .

    Article Snippet: Cells were scraped into 1.5-ml microfuge tubes and were centrifuged at 12,000 g for 5 min (model Microfuge R; Beckman Coulter, Fullerton, CA).

    Techniques: Solubility, Expressing, Pulse Chase, Immunoprecipitation, Polyacrylamide Gel Electrophoresis, Labeling