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  • 79
    Thermo Fisher microcentrifuge tubes
    The effect of exosite 2-directed ligands on 125 I-YPR-α-thrombin binding to γA /γA -fibrin clots. 20 n m 125 I-YPR-α-thrombin and 5 n m α-thrombin were added to <t>microcentrifuge</t> tubes containing 2 μ m γA /γA -fibrinogen, 2 m m CaCl2 , and HD22 (●), γ′-peptide (▴), or F2 (■) at the indicated concentrations. After incubation for 45 min, clots were pelleted by centrifugation, and free 125 I-YPR-α-thrombin in the supernatant was used to calculate the bound fraction. Data are plotted as the relative amount of thrombin bound to the clot versus the concentration of exosite-directed ligand and represent the means ± S.E. of three experiments, each done in duplicate, while the lines represent non-linear regression analysis of the data. HD22 was the most potent of the three ligands, having a Ki obs of 673 ± 83 n m . γ′-Peptide and F2 are less potent than HD22, and reduce thrombin binding with Ki obs values of 15.6 ± 3.8 and 18.5 ± 3.3 μ m , respectively.
    Microcentrifuge Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/Thermo Fisher
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2019-10
    79/100 stars
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    79
    Millipore microcentrifuge tubes
    A cartoon schematic representing the apple tissue decellularization and mammalian cell seeding protocol used in this study. A) McIntosh Red apples were exposed to −20°C temperatures for a max duration of 5 minutes, to increase the firmness of the outer apple hypanthium tissue. B) Uniform 1.2±0.1 mm thick slices of the apples were obtained using a mandolin slicer. Slices containing any of the ovary core of the apple were removed. C) The apple slices were cut into uniform 2.0 by 0.5 cm segments that were placed in individual <t>microcentrifuge</t> tubes. D) A 0.5% SDS solution was added to the microcentrifuge tubes and placed on a shaker for 12 hours at room temperature. The scaffolds were then rinsed repeatedly with PBS and allowed to incubate in a PBS solution with 1% streptomycin/penicillin and 1% amphotericin B for 6 hours at room temperature. E) The scaffolds were then coated with Type 1 collagen, chemically cross linked with glutaraldehyde or incubated in PBS. F) All the samples were then incubated in mammalian cell culture medium (DMEM) for 12 hours in a standard tissue culture incubator maintained at 37°C and 5% CO 2 . G) The scaffolds were placed in PDMS coated 24 well plates and a 40 µL cell suspension was placed on each. After 6 hours in the incubator the wells were filled with DMEM and cells cultured for up to 12 weeks.
    Microcentrifuge Tubes, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2019-10
    79/100 stars
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    99
    Eppendorf AG microcentrifuge tube
    Preparation of two-layer Percoll gradient in 2 ml conical <t>microcentrifuge</t> tube. The 0% Percoll layer containing the opalescent sample (L1) is overlaid onto the 15% Percoll solution (L2) using an extremely low flow rate. To do so, the pipette tip should be first conducted along the tube wall to build a narrow fluid bridge that helps the suspension (L1) of sample to flow gently on top of the L2 layer.
    Microcentrifuge Tube, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tube/product/Eppendorf AG
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tube - by Bioz Stars, 2019-10
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    79
    Corning Life Sciences microcentrifuge tubes
    Basic laboratory reagents and supplies needed to evaluate/discriminate the blood source from engorged mosquitoes. Materials are: 1. Tulle fabric; 2. Rounded container; 3. Glass beaker containing distilled water; 4. 96-well plate, flat bottom; 5. 1 mL syringe with needle; 6. plastic pestle; 7. 1.7 mL <t>microcentrifuge</t> tube; 8. 20–200 µL universal tip; 9. 15 mL tube; 10. EB solution; 11. 20–200 µL pipette.
    Microcentrifuge Tubes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/Corning Life Sciences
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2019-10
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    79
    USA Scientific Inc microcentrifuge tubes
    Basic laboratory reagents and supplies needed to evaluate/discriminate the blood source from engorged mosquitoes. Materials are: 1. Tulle fabric; 2. Rounded container; 3. Glass beaker containing distilled water; 4. 96-well plate, flat bottom; 5. 1 mL syringe with needle; 6. plastic pestle; 7. 1.7 mL <t>microcentrifuge</t> tube; 8. 20–200 µL universal tip; 9. 15 mL tube; 10. EB solution; 11. 20–200 µL pipette.
    Microcentrifuge Tubes, supplied by USA Scientific Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/USA Scientific Inc
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2019-10
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    77
    Thermo Fisher non sticky rnase free microcentrifuge tubes
    <t>RNase</t> A detection workflow. First, a nanopore experiment is conducted and at the end the solution from each chamber is collected and transferred to a <t>microcentrifuge</t> tube. Second, mRNA is added to the solution collected in step 1 and incubated for 24°C. Third, after incubation the solution from step 2 is used as source of template RNA for RT-PCR reaction. Fourth, RT-PCR is performed. In the fifth step, the end product from RT-PCR is run on an agarose gel. If there is RNase A present in solutions collected in step 1 then there will be a faint band or no band (depending on RNase A quantity) on the agarose gel.
    Non Sticky Rnase Free Microcentrifuge Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non sticky rnase free microcentrifuge tubes/product/Thermo Fisher
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    non sticky rnase free microcentrifuge tubes - by Bioz Stars, 2019-10
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    82
    Corning Life Sciences maxyclear snaplock microcentrifuge tube
    Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of <t>microcentrifuge</t> tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. <t>MaxyClear,</t> Axygen® 1.7 mL MaxyClear <t>Snaplock</t> Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Maxyclear Snaplock Microcentrifuge Tube, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 82/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxyclear snaplock microcentrifuge tube/product/Corning Life Sciences
    Average 82 stars, based on 4 article reviews
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    maxyclear snaplock microcentrifuge tube - by Bioz Stars, 2019-10
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    78
    Thermo Fisher microcentrifuge tube
    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a <t>microcentrifuge</t> tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .
    Microcentrifuge Tube, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tube/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tube - by Bioz Stars, 2019-10
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    Image Search Results


    The effect of exosite 2-directed ligands on 125 I-YPR-α-thrombin binding to γA /γA -fibrin clots. 20 n m 125 I-YPR-α-thrombin and 5 n m α-thrombin were added to microcentrifuge tubes containing 2 μ m γA /γA -fibrinogen, 2 m m CaCl2 , and HD22 (●), γ′-peptide (▴), or F2 (■) at the indicated concentrations. After incubation for 45 min, clots were pelleted by centrifugation, and free 125 I-YPR-α-thrombin in the supernatant was used to calculate the bound fraction. Data are plotted as the relative amount of thrombin bound to the clot versus the concentration of exosite-directed ligand and represent the means ± S.E. of three experiments, each done in duplicate, while the lines represent non-linear regression analysis of the data. HD22 was the most potent of the three ligands, having a Ki obs of 673 ± 83 n m . γ′-Peptide and F2 are less potent than HD22, and reduce thrombin binding with Ki obs values of 15.6 ± 3.8 and 18.5 ± 3.3 μ m , respectively.

    Journal:

    Article Title: Long Range Communication between Exosites 1 and 2 Modulates Thrombin Function

    doi: 10.1074/jbc.M109.000042

    Figure Lengend Snippet: The effect of exosite 2-directed ligands on 125 I-YPR-α-thrombin binding to γA /γA -fibrin clots. 20 n m 125 I-YPR-α-thrombin and 5 n m α-thrombin were added to microcentrifuge tubes containing 2 μ m γA /γA -fibrinogen, 2 m m CaCl2 , and HD22 (●), γ′-peptide (▴), or F2 (■) at the indicated concentrations. After incubation for 45 min, clots were pelleted by centrifugation, and free 125 I-YPR-α-thrombin in the supernatant was used to calculate the bound fraction. Data are plotted as the relative amount of thrombin bound to the clot versus the concentration of exosite-directed ligand and represent the means ± S.E. of three experiments, each done in duplicate, while the lines represent non-linear regression analysis of the data. HD22 was the most potent of the three ligands, having a Ki obs of 673 ± 83 n m . γ′-Peptide and F2 are less potent than HD22, and reduce thrombin binding with Ki obs values of 15.6 ± 3.8 and 18.5 ± 3.3 μ m , respectively.

    Article Snippet: Briefly, 10 μ m γA /γA -fibrinogen, 17 n m 125 I-YPR-α-thrombin, and 15 n m factor XIII in HBS with 0.005% Tween 20 were added to microcentrifuge tubes containing a plastic inoculation loop (Bac-Loop, Thermo-Fisher Scientific, Waltham, MA).

    Techniques: Binding Assay, Incubation, Centrifugation, Concentration Assay

    Effect of HD22 on the affinity of thrombin for γA /γA -fibrin clots. 20 n m 125 I-YPR-α-thrombin and 5 n m α-thrombin were added to a series of microcentrifuge tubes containing 0–12.5 μ m γA /γA -fibrinogen and 2 m m CaCl2 in the absence (♦) or presence of 20 μ m HD22 (●). After incubation for 45 min, fibrin was pelleted by centrifugation, and free 125 I-YPR-α-thrombin in the supernatant was used to calculate the bound fraction. Data are plotted as the relative amount of thrombin bound to the fibrin clot versus the γA /γA -fibrin concentration and represent the means ± S.E. of three experiments, each done in duplicate, while the lines represent non-linear regression analysis of the data. The affinity of 125 I-YPR-α-thrombin for γA /γA -fibrin is 6-fold lower in the presence of HD22 than it is in its absence ( Kd values of 12.6 ± 1.4 and 2.1 ± 0.7 μ m , respectively).

    Journal:

    Article Title: Long Range Communication between Exosites 1 and 2 Modulates Thrombin Function

    doi: 10.1074/jbc.M109.000042

    Figure Lengend Snippet: Effect of HD22 on the affinity of thrombin for γA /γA -fibrin clots. 20 n m 125 I-YPR-α-thrombin and 5 n m α-thrombin were added to a series of microcentrifuge tubes containing 0–12.5 μ m γA /γA -fibrinogen and 2 m m CaCl2 in the absence (♦) or presence of 20 μ m HD22 (●). After incubation for 45 min, fibrin was pelleted by centrifugation, and free 125 I-YPR-α-thrombin in the supernatant was used to calculate the bound fraction. Data are plotted as the relative amount of thrombin bound to the fibrin clot versus the γA /γA -fibrin concentration and represent the means ± S.E. of three experiments, each done in duplicate, while the lines represent non-linear regression analysis of the data. The affinity of 125 I-YPR-α-thrombin for γA /γA -fibrin is 6-fold lower in the presence of HD22 than it is in its absence ( Kd values of 12.6 ± 1.4 and 2.1 ± 0.7 μ m , respectively).

    Article Snippet: Briefly, 10 μ m γA /γA -fibrinogen, 17 n m 125 I-YPR-α-thrombin, and 15 n m factor XIII in HBS with 0.005% Tween 20 were added to microcentrifuge tubes containing a plastic inoculation loop (Bac-Loop, Thermo-Fisher Scientific, Waltham, MA).

    Techniques: Incubation, Centrifugation, Concentration Assay

    Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Journal: Archives of biochemistry and biophysics

    Article Title: The invertebrate Caenorhabditis elegans biosynthesizes ascorbate

    doi: 10.1016/j.abb.2015.02.002

    Figure Lengend Snippet: Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Article Snippet: Approximately 250 µl of worms were transferred to microcentrifuge tubes with ethanol (Acros, 200 proof, catalog # 61509-0010) diluted in M9 medium.

    Techniques: Incubation, Gas Chromatography, Mass Spectrometry, Two Tailed Test, High Performance Liquid Chromatography

    A cartoon schematic representing the apple tissue decellularization and mammalian cell seeding protocol used in this study. A) McIntosh Red apples were exposed to −20°C temperatures for a max duration of 5 minutes, to increase the firmness of the outer apple hypanthium tissue. B) Uniform 1.2±0.1 mm thick slices of the apples were obtained using a mandolin slicer. Slices containing any of the ovary core of the apple were removed. C) The apple slices were cut into uniform 2.0 by 0.5 cm segments that were placed in individual microcentrifuge tubes. D) A 0.5% SDS solution was added to the microcentrifuge tubes and placed on a shaker for 12 hours at room temperature. The scaffolds were then rinsed repeatedly with PBS and allowed to incubate in a PBS solution with 1% streptomycin/penicillin and 1% amphotericin B for 6 hours at room temperature. E) The scaffolds were then coated with Type 1 collagen, chemically cross linked with glutaraldehyde or incubated in PBS. F) All the samples were then incubated in mammalian cell culture medium (DMEM) for 12 hours in a standard tissue culture incubator maintained at 37°C and 5% CO 2 . G) The scaffolds were placed in PDMS coated 24 well plates and a 40 µL cell suspension was placed on each. After 6 hours in the incubator the wells were filled with DMEM and cells cultured for up to 12 weeks.

    Journal: PLoS ONE

    Article Title: Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture

    doi: 10.1371/journal.pone.0097835

    Figure Lengend Snippet: A cartoon schematic representing the apple tissue decellularization and mammalian cell seeding protocol used in this study. A) McIntosh Red apples were exposed to −20°C temperatures for a max duration of 5 minutes, to increase the firmness of the outer apple hypanthium tissue. B) Uniform 1.2±0.1 mm thick slices of the apples were obtained using a mandolin slicer. Slices containing any of the ovary core of the apple were removed. C) The apple slices were cut into uniform 2.0 by 0.5 cm segments that were placed in individual microcentrifuge tubes. D) A 0.5% SDS solution was added to the microcentrifuge tubes and placed on a shaker for 12 hours at room temperature. The scaffolds were then rinsed repeatedly with PBS and allowed to incubate in a PBS solution with 1% streptomycin/penicillin and 1% amphotericin B for 6 hours at room temperature. E) The scaffolds were then coated with Type 1 collagen, chemically cross linked with glutaraldehyde or incubated in PBS. F) All the samples were then incubated in mammalian cell culture medium (DMEM) for 12 hours in a standard tissue culture incubator maintained at 37°C and 5% CO 2 . G) The scaffolds were placed in PDMS coated 24 well plates and a 40 µL cell suspension was placed on each. After 6 hours in the incubator the wells were filled with DMEM and cells cultured for up to 12 weeks.

    Article Snippet: Individual apple tissue samples were placed in sterilized 2.5 mL microcentrifuge tubes and 2 mL of 0.5% sodium dodecyl sulphate (SDS) (Sigma-Aldrich) solution was added to each tube.

    Techniques: Incubation, Cell Culture

    Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Journal: Archives of biochemistry and biophysics

    Article Title: The invertebrate Caenorhabditis elegans biosynthesizes ascorbate

    doi: 10.1016/j.abb.2015.02.002

    Figure Lengend Snippet: Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Article Snippet: After obtaining the animals as described above, pellets of worms were transferred to a microcentrifuge tubes and 150–300 µl of GC-MS extraction buffer (0.5 mM butylated hydroxytoluene (BHT, Sigma, catalog # B1378) in methanol) was added.

    Techniques: Incubation, Gas Chromatography, Mass Spectrometry, Two Tailed Test, High Performance Liquid Chromatography

    Preparation of two-layer Percoll gradient in 2 ml conical microcentrifuge tube. The 0% Percoll layer containing the opalescent sample (L1) is overlaid onto the 15% Percoll solution (L2) using an extremely low flow rate. To do so, the pipette tip should be first conducted along the tube wall to build a narrow fluid bridge that helps the suspension (L1) of sample to flow gently on top of the L2 layer.

    Journal: MethodsX

    Article Title: Preparation of purified perikaryal and synaptosomal mitochondrial fractions from relatively small hypothalamic brain samples

    doi: 10.1016/j.mex.2016.05.004

    Figure Lengend Snippet: Preparation of two-layer Percoll gradient in 2 ml conical microcentrifuge tube. The 0% Percoll layer containing the opalescent sample (L1) is overlaid onto the 15% Percoll solution (L2) using an extremely low flow rate. To do so, the pipette tip should be first conducted along the tube wall to build a narrow fluid bridge that helps the suspension (L1) of sample to flow gently on top of the L2 layer.

    Article Snippet: After homogenization, all buffer that contains mitochondria and cell debris has to be recollected from the homogenizer and put into a microcentrifuge tube (1.5 ml Eppendorf tube).

    Techniques: Flow Cytometry, Transferring

    Influence of standing saliva samples at RT on stability of 3HB ( a ), 3HIB ( b ), 3HMB ( c ), and 2HB ( d ). Saliva samples collected from healthy volunteers ( n = 4) were aliquoted into seven microcentrifuge tubes and standing at RT for various times (0, 1, 2, 4, 6, 10, and 24 h). Thereafter, the saliva samples were stored at −20 °C until analysis. RT room temperature. See legends of Figs. 1 , 2 , and 3 for other abbreviations. Data are shown as mean ± SEM. Statistical analysis by one-way ANOVA multiple comparison and a post hoc Dunnett’s multiple comparison test. ANOVA P values are a P = 0.9949, b P = 0.9968, c P = 0.7058, and d P

    Journal: SpringerPlus

    Article Title: Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as possible markers of amino acid and fatty acid catabolic pathways by LC–ESI–MS/MS

    doi: 10.1186/s40064-015-1304-0

    Figure Lengend Snippet: Influence of standing saliva samples at RT on stability of 3HB ( a ), 3HIB ( b ), 3HMB ( c ), and 2HB ( d ). Saliva samples collected from healthy volunteers ( n = 4) were aliquoted into seven microcentrifuge tubes and standing at RT for various times (0, 1, 2, 4, 6, 10, and 24 h). Thereafter, the saliva samples were stored at −20 °C until analysis. RT room temperature. See legends of Figs. 1 , 2 , and 3 for other abbreviations. Data are shown as mean ± SEM. Statistical analysis by one-way ANOVA multiple comparison and a post hoc Dunnett’s multiple comparison test. ANOVA P values are a P = 0.9949, b P = 0.9968, c P = 0.7058, and d P

    Article Snippet: In addition, to examine the influence of standing sample at room temperature (RT) on stability of four examined hydroxybutyrates, collected saliva samples from healthy volunteers (n = 4) were aliquoted into a microcentrifuge tube (1.5 mL, Eppendorf, Hamburg, Germany) and kept in incubator set at 25 °C for 0, 1, 2, 4, 6, 10, and 24 h, and then, transferred at −20 °C.

    Techniques:

    Growth promotion of tobacco by exposure to TC09. (A) Control tobacco plants without fungal exposure (left) and plants exposed to physically separated TC09 contained in filter-sealed microcentrifuge tubes (right). Arrows indicated the fungus with dark-colored mycelium. Photograph was taken 10 days after fungal exposure. (B) Tobacco plants exposed to TC09 contained in open-end tube closures. Images were taken 20 days after introduction of fungal cultures. Left and upper row: control plants without TC09 exposure in triplicates per vessel, Left and lower row: plants with exposure to fungal cultures in triplicates per vessel. Arrows indicate TC09 mycelium in closure. Right panel: representative plants of control (left) and TC09-exposed treatment (right). (C) Evaluation of plant growth in greenhouse following in vitro seedling treatment with or without TC09 exposure. Plants with (right three) or without (left two) exposure to TC09 for 20 days were transplanted to soil and maintained in greenhouse using standard management practices. Photograph was taken 70 days after seed sowing.

    Journal: Frontiers in Plant Science

    Article Title: Exposure in vitro to an Environmentally Isolated Strain TC09 of Cladosporium sphaerospermum Triggers Plant Growth Promotion, Early Flowering, and Fruit Yield Increase

    doi: 10.3389/fpls.2018.01959

    Figure Lengend Snippet: Growth promotion of tobacco by exposure to TC09. (A) Control tobacco plants without fungal exposure (left) and plants exposed to physically separated TC09 contained in filter-sealed microcentrifuge tubes (right). Arrows indicated the fungus with dark-colored mycelium. Photograph was taken 10 days after fungal exposure. (B) Tobacco plants exposed to TC09 contained in open-end tube closures. Images were taken 20 days after introduction of fungal cultures. Left and upper row: control plants without TC09 exposure in triplicates per vessel, Left and lower row: plants with exposure to fungal cultures in triplicates per vessel. Arrows indicate TC09 mycelium in closure. Right panel: representative plants of control (left) and TC09-exposed treatment (right). (C) Evaluation of plant growth in greenhouse following in vitro seedling treatment with or without TC09 exposure. Plants with (right three) or without (left two) exposure to TC09 for 20 days were transplanted to soil and maintained in greenhouse using standard management practices. Photograph was taken 70 days after seed sowing.

    Article Snippet: First, aliquots of 300 μl warm MS medium were poured into 1.5 ml Eppendorf microcentrifuge tubes.

    Techniques: In Vitro

    Relaxation times of SPIONdex concentration series. The 0.2 mL microcentrifuge tubes were filled with 0, 1:10,000, and 1:1,000 SPIONdex (7.57 mg Fe/mL stock solution) in 1% agarose gel and embedded in 5% agarose gel. Notes: ( A ) Color-coded maps of agarose-embedded SPIONdex show reduced T 1 -, T 2 -, and T 2 *-times with the increasing iron content. Diameter of the vials shown =8 mm. ( B ) T 1 -, ( C ) T 2 - and ( D ) T 2 *-relaxation times. Data are expressed as mean ± standard deviation of quadruple measurements. * P

    Journal: International Journal of Nanomedicine

    Article Title: Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging

    doi: 10.2147/IJN.S138108

    Figure Lengend Snippet: Relaxation times of SPIONdex concentration series. The 0.2 mL microcentrifuge tubes were filled with 0, 1:10,000, and 1:1,000 SPIONdex (7.57 mg Fe/mL stock solution) in 1% agarose gel and embedded in 5% agarose gel. Notes: ( A ) Color-coded maps of agarose-embedded SPIONdex show reduced T 1 -, T 2 -, and T 2 *-times with the increasing iron content. Diameter of the vials shown =8 mm. ( B ) T 1 -, ( C ) T 2 - and ( D ) T 2 *-relaxation times. Data are expressed as mean ± standard deviation of quadruple measurements. * P

    Article Snippet: For measurement, nanoparticle dilutions were molded in 1% agarose within 0.2 mL microcentrifuge tubes (Eppendorf, Hamburg, Germany) to obtain the test concentrations [0, 1:1, 1:1,000, and 1:10,000 SPIONdex (7.57 mg/mL Fe)].

    Techniques: Concentration Assay, Agarose Gel Electrophoresis, Standard Deviation

    Palmitate contamination from plasticware affects quantitative analysis. a Nanomoles of palmitate detected in: methanol from plastic microcentrifuge tubes that were bath sonicated, methanol incubated in plastic microcentrifuge tubes (no sonication), methanol

    Journal:

    Article Title: Inaccurate quantitation of palmitate in metabolomics and isotope tracer studies due to plastics

    doi: 10.1007/s11306-016-1081-y

    Figure Lengend Snippet: Palmitate contamination from plasticware affects quantitative analysis. a Nanomoles of palmitate detected in: methanol from plastic microcentrifuge tubes that were bath sonicated, methanol incubated in plastic microcentrifuge tubes (no sonication), methanol

    Article Snippet: We tested polypropylene microcentrifuge tubes from three vendors (Eppendorf, Fisherbrand, and VWR) and polypropylene 1000 μL pipette tips from three vendors (Fisherbrand, VWR, and Thermo) to evaluate palmitate and stearate contamination.

    Techniques: Sonication, Incubation

    Standard curves of measured palmitate concentration versus actual palmitate concentration for samples extracted with plastic microcentrifuge tubes and plastic pipettes ( a ), all glassware ( b ), or plastic microcentrifuge tubes rinsed with methanol and glass

    Journal:

    Article Title: Inaccurate quantitation of palmitate in metabolomics and isotope tracer studies due to plastics

    doi: 10.1007/s11306-016-1081-y

    Figure Lengend Snippet: Standard curves of measured palmitate concentration versus actual palmitate concentration for samples extracted with plastic microcentrifuge tubes and plastic pipettes ( a ), all glassware ( b ), or plastic microcentrifuge tubes rinsed with methanol and glass

    Article Snippet: We tested polypropylene microcentrifuge tubes from three vendors (Eppendorf, Fisherbrand, and VWR) and polypropylene 1000 μL pipette tips from three vendors (Fisherbrand, VWR, and Thermo) to evaluate palmitate and stearate contamination.

    Techniques: Concentration Assay

    3.2 Measuring contamination from plastic microcentrifuge tubes

    Journal:

    Article Title: Inaccurate quantitation of palmitate in metabolomics and isotope tracer studies due to plastics

    doi: 10.1007/s11306-016-1081-y

    Figure Lengend Snippet: 3.2 Measuring contamination from plastic microcentrifuge tubes

    Article Snippet: We tested polypropylene microcentrifuge tubes from three vendors (Eppendorf, Fisherbrand, and VWR) and polypropylene 1000 μL pipette tips from three vendors (Fisherbrand, VWR, and Thermo) to evaluate palmitate and stearate contamination.

    Techniques:

    Doxorubicin remaining in solution in polypropylene microcentrifuge tubes at 37°C over 48 h at pH 4.8 a , pH 6.5 b , and pH 7.4 c . Each value represents the mean ± SD ( n = 4).

    Journal:

    Article Title: Adsorption and Degradation of Doxorubicin from Aqueous Solution in Polypropylene Containers

    doi: 10.1208/s12249-012-9885-1

    Figure Lengend Snippet: Doxorubicin remaining in solution in polypropylene microcentrifuge tubes at 37°C over 48 h at pH 4.8 a , pH 6.5 b , and pH 7.4 c . Each value represents the mean ± SD ( n = 4).

    Article Snippet: Doxorubicin-buffered solutions at pH values of 4.8, 6.5, and 7.4 were evaluated for adsorption and degradation at 37°C in polypropylene microcentrifuge tubes (Eppendorf, cat. no. 022363352, Hauppauge, New York).

    Techniques:

    Basic laboratory reagents and supplies needed to evaluate/discriminate the blood source from engorged mosquitoes. Materials are: 1. Tulle fabric; 2. Rounded container; 3. Glass beaker containing distilled water; 4. 96-well plate, flat bottom; 5. 1 mL syringe with needle; 6. plastic pestle; 7. 1.7 mL microcentrifuge tube; 8. 20–200 µL universal tip; 9. 15 mL tube; 10. EB solution; 11. 20–200 µL pipette.

    Journal: PLoS ONE

    Article Title: Evans Blue as a Simple Method to Discriminate Mosquitoes’ Feeding Choice on Small Laboratory Animals

    doi: 10.1371/journal.pone.0110551

    Figure Lengend Snippet: Basic laboratory reagents and supplies needed to evaluate/discriminate the blood source from engorged mosquitoes. Materials are: 1. Tulle fabric; 2. Rounded container; 3. Glass beaker containing distilled water; 4. 96-well plate, flat bottom; 5. 1 mL syringe with needle; 6. plastic pestle; 7. 1.7 mL microcentrifuge tube; 8. 20–200 µL universal tip; 9. 15 mL tube; 10. EB solution; 11. 20–200 µL pipette.

    Article Snippet: Dead mosquitoes were individually placed in 1.7 mL microcentrifuge tubes (Corning Life Sciences – Axygen Inc., Union City, CA, USA) containing 250 µL of distilled water and grinded with small plastic disposable pestles (Corning – Axygen) until total homogenization.

    Techniques: Transferring

    RNase A detection workflow. First, a nanopore experiment is conducted and at the end the solution from each chamber is collected and transferred to a microcentrifuge tube. Second, mRNA is added to the solution collected in step 1 and incubated for 24°C. Third, after incubation the solution from step 2 is used as source of template RNA for RT-PCR reaction. Fourth, RT-PCR is performed. In the fifth step, the end product from RT-PCR is run on an agarose gel. If there is RNase A present in solutions collected in step 1 then there will be a faint band or no band (depending on RNase A quantity) on the agarose gel.

    Journal: PLoS ONE

    Article Title: RNase A Does Not Translocate the Alpha-Hemolysin Pore

    doi: 10.1371/journal.pone.0088004

    Figure Lengend Snippet: RNase A detection workflow. First, a nanopore experiment is conducted and at the end the solution from each chamber is collected and transferred to a microcentrifuge tube. Second, mRNA is added to the solution collected in step 1 and incubated for 24°C. Third, after incubation the solution from step 2 is used as source of template RNA for RT-PCR reaction. Fourth, RT-PCR is performed. In the fifth step, the end product from RT-PCR is run on an agarose gel. If there is RNase A present in solutions collected in step 1 then there will be a faint band or no band (depending on RNase A quantity) on the agarose gel.

    Article Snippet: After 1 hr incubation, 245 µL of the electrolyte solution was collected from each chamber and transferred to non-sticky RNase-free microcentrifuge tubes (Life Technologies, Burlington, ON).

    Techniques: Incubation, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube

    Article Snippet: The following tubes were tested: Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube (Cat. # MCT-175-C) (Corning, New York), Eppendorf DNA LoBind Snap Cap PCR Tube (Cat. # 022431021) (Hauppauge, NY), Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube (Cat. # 02–681-331) (Waltham, MA), and Fisherbrand™ Premium Microcentrifuge Tube (Cat. # 05–408-129).

    Techniques: Purification, Chromatin Immunoprecipitation, Concentration Assay, Sensitive Assay, Polymerase Chain Reaction

    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a microcentrifuge tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .

    Journal: Journal of Proteome Research

    Article Title: An Improved Method for Measuring Absolute Metabolite Concentrations in Small Biofluid or Tissue Samples

    doi: 10.1021/acs.jproteome.8b00773

    Figure Lengend Snippet: The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a microcentrifuge tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .

    Article Snippet: For the “unopened” or “control” sample, after 1 min of contact with the larvae, 7.5 μL (V t ) of the sodium formate-saline solution was transferred with a Hamilton syringe to 100 μL deionized water in a 0.22 μm filter unit and spun into a microcentrifuge tube for 1 min at 13 000 rpm (Thermo Scientific, 75002425).

    Techniques: Concentration Assay

    The pVDTS workflow for liquid hemolymph samples. An accurately measured volume ( V f ) of saline containing the chosen standard (sodium 13 C-formate) at a known concentration [ f ] 0 is transferred to a number of larvae ( n ), with a collective hemolymph volume V h (a). An accurately measured volume of the droplet ( V t ) is removed and transferred into water in a microcentrifuge column (b)—this constitutes the origin of the control “unopened” sample. Larval cuticles are then ruptured to release hemolymph into the droplet (c), and a second accurately measured volume ( V t ) is removed from the droplet and transferred to water in a second microcentrifuge column (d). The following steps are then performed in parallel for the two samplings: microcentrifuge tubes are spun to remove debris and clear hemocytes from the “opened” sample (e). The cleared filtrates are then each transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (f). After further separation of polar and nonpolar components via the Bligh–Dyer method (g), the upper aqueous phases containing polar species are aspirated to a second pair of microcentrifuge tubes (h). The solutions are evaporated to dryness (i) and the residues suspended in D 2 O (j) prior to transfer to a pair of NMR tubes (k). V h : Volume of released hemolymph. [X] h : Concentration of metabolite X in the hemolymph. I ′ 1 f : Intensity of 1 H resonance of the 13 C-formate standard when larvae do not have their cuticles ruptured (the “unopened” sample) in units relative the internal DSS concentration. I ′ 2 f : 1 H resonance of the standard when larvae have their cuticles ruptured (the “opened” sample).

    Journal: Journal of Proteome Research

    Article Title: An Improved Method for Measuring Absolute Metabolite Concentrations in Small Biofluid or Tissue Samples

    doi: 10.1021/acs.jproteome.8b00773

    Figure Lengend Snippet: The pVDTS workflow for liquid hemolymph samples. An accurately measured volume ( V f ) of saline containing the chosen standard (sodium 13 C-formate) at a known concentration [ f ] 0 is transferred to a number of larvae ( n ), with a collective hemolymph volume V h (a). An accurately measured volume of the droplet ( V t ) is removed and transferred into water in a microcentrifuge column (b)—this constitutes the origin of the control “unopened” sample. Larval cuticles are then ruptured to release hemolymph into the droplet (c), and a second accurately measured volume ( V t ) is removed from the droplet and transferred to water in a second microcentrifuge column (d). The following steps are then performed in parallel for the two samplings: microcentrifuge tubes are spun to remove debris and clear hemocytes from the “opened” sample (e). The cleared filtrates are then each transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (f). After further separation of polar and nonpolar components via the Bligh–Dyer method (g), the upper aqueous phases containing polar species are aspirated to a second pair of microcentrifuge tubes (h). The solutions are evaporated to dryness (i) and the residues suspended in D 2 O (j) prior to transfer to a pair of NMR tubes (k). V h : Volume of released hemolymph. [X] h : Concentration of metabolite X in the hemolymph. I ′ 1 f : Intensity of 1 H resonance of the 13 C-formate standard when larvae do not have their cuticles ruptured (the “unopened” sample) in units relative the internal DSS concentration. I ′ 2 f : 1 H resonance of the standard when larvae have their cuticles ruptured (the “opened” sample).

    Article Snippet: For the “unopened” or “control” sample, after 1 min of contact with the larvae, 7.5 μL (V t ) of the sodium formate-saline solution was transferred with a Hamilton syringe to 100 μL deionized water in a 0.22 μm filter unit and spun into a microcentrifuge tube for 1 min at 13 000 rpm (Thermo Scientific, 75002425).

    Techniques: Concentration Assay, Nuclear Magnetic Resonance