microcentrifuge tubes Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    BrandTech microcentrifuge tubes
    Microcentrifuge Tubes, supplied by BrandTech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/BrandTech
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher microcentrifuge tubes
    Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a <t>microcentrifuge</t> tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.
    Microcentrifuge Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 622 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/Thermo Fisher
    Average 99 stars, based on 622 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Millipore microcentrifuge tubes
    Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a <t>microcentrifuge</t> tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.
    Microcentrifuge Tubes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tubes/product/Millipore
    Average 99 stars, based on 483 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tubes - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher microcentrifuge tube
    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a <t>microcentrifuge</t> tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .
    Microcentrifuge Tube, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tube/product/Thermo Fisher
    Average 94 stars, based on 1285 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tube - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    93
    BrandTech microcentrifuge tube
    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a <t>microcentrifuge</t> tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .
    Microcentrifuge Tube, supplied by BrandTech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tube/product/BrandTech
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tube - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    94
    Millipore microcentrifuge
    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a <t>microcentrifuge</t> tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .
    Microcentrifuge, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge/product/Millipore
    Average 94 stars, based on 389 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    99
    Millipore clean microcentrifuge tubes
    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a <t>microcentrifuge</t> tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .
    Clean Microcentrifuge Tubes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clean microcentrifuge tubes/product/Millipore
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    clean microcentrifuge tubes - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Journal: Archives of biochemistry and biophysics

    Article Title: The invertebrate Caenorhabditis elegans biosynthesizes ascorbate

    doi: 10.1016/j.abb.2015.02.002

    Figure Lengend Snippet: Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Article Snippet: Approximately 250 µl of worms were transferred to microcentrifuge tubes with ethanol (Acros, 200 proof, catalog # 61509-0010) diluted in M9 medium.

    Techniques: Incubation, Gas Chromatography-Mass Spectrometry, Two Tailed Test, High Performance Liquid Chromatography

    Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Journal: Archives of biochemistry and biophysics

    Article Title: The invertebrate Caenorhabditis elegans biosynthesizes ascorbate

    doi: 10.1016/j.abb.2015.02.002

    Figure Lengend Snippet: Paraquat and ethanol stress do not increase ascorbate levels in C. elegans (A) A mixed population of animals was obtained from approximately six NGM plates and incubated in S-medium supplemented with OP50 E. coli and 0, 50, 100, and 200 µM paraquat for 7 d as described in the “Materials and Methods.” GC-MS analysis was performed as described in the “Materials and Methods” and the amount of ascorbate in samples was analyzed by comparing the EIC area of ascorbate (332 m/z) to that of serine (204 m/z). The points represent four independent samples, the horizontal line is the average, and the error bars denote standard deviations. Statistical significance was determined using an unpaired, two-tailed Student’s t-test. (B) Mixed populations of animals from 80 NGM plates were incubated in a microcentrifuge tube at 20 °C and 160 rpm in M9 medium supplemented with 0, 1%, 5%, or 10% ethanol. After incubation for 39 h, the animals were pelleted, washed, lysed, and analyzed by reversed-phase HPLC according to the “Materials and Methods” section. The data points represent two independent samples and the horizontal line denotes the average. Statistical significance was determined using an unpaired, two-tailed Student’s t-test.

    Article Snippet: Gas chromatography-mass spectrometry (GC-MS) analysis After obtaining the animals as described above, pellets of worms were transferred to a microcentrifuge tubes and 150–300 µl of GC-MS extraction buffer (0.5 mM butylated hydroxytoluene (BHT, Sigma, catalog # B1378) in methanol) was added.

    Techniques: Incubation, Gas Chromatography-Mass Spectrometry, Two Tailed Test, High Performance Liquid Chromatography

    The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a microcentrifuge tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .

    Journal: Journal of Proteome Research

    Article Title: An Improved Method for Measuring Absolute Metabolite Concentrations in Small Biofluid or Tissue Samples

    doi: 10.1021/acs.jproteome.8b00773

    Figure Lengend Snippet: The pVDTS workflow for solid whole larval samples. An accurately measured volume ( V f ) of saline containing 13 C-formate at a known concentration [ f ] 0 is transferred to a number of larvae ( n ) in a microcentrifuge tube (a). An accurately measured volume ( V t ) is then removed from the larvae in the microcentrifuge tube for the “unopened” sample (b). Larvae are then homogenized using a motorized pellet pestle and the microcentrifuge tube as a mortar (c)—this will be the “opened” sample. An accurately measured volume ( V t ) is then removed from the homogenized larvae and transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (d). The remaining steps (e–i) are the same as (e–i) in Figure 1 . V WL : volume of homogenized whole larva. [X] WL : concentration of metabolite X in whole larval homogenate. I ′ 1 f and I ′ 2 f : “unopened” and “opened” sample signal intensities as per Figure 1 .

    Article Snippet: For the “unopened” or “control” sample, after 1 min of contact with the larvae, 7.5 μL (V t ) of the sodium formate-saline solution was transferred with a Hamilton syringe to 100 μL deionized water in a 0.22 μm filter unit and spun into a microcentrifuge tube for 1 min at 13 000 rpm (Thermo Scientific, 75002425).

    Techniques: Concentration Assay

    The pVDTS workflow for liquid hemolymph samples. An accurately measured volume ( V f ) of saline containing the chosen standard (sodium 13 C-formate) at a known concentration [ f ] 0 is transferred to a number of larvae ( n ), with a collective hemolymph volume V h (a). An accurately measured volume of the droplet ( V t ) is removed and transferred into water in a microcentrifuge column (b)—this constitutes the origin of the control “unopened” sample. Larval cuticles are then ruptured to release hemolymph into the droplet (c), and a second accurately measured volume ( V t ) is removed from the droplet and transferred to water in a second microcentrifuge column (d). The following steps are then performed in parallel for the two samplings: microcentrifuge tubes are spun to remove debris and clear hemocytes from the “opened” sample (e). The cleared filtrates are then each transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (f). After further separation of polar and nonpolar components via the Bligh–Dyer method (g), the upper aqueous phases containing polar species are aspirated to a second pair of microcentrifuge tubes (h). The solutions are evaporated to dryness (i) and the residues suspended in D 2 O (j) prior to transfer to a pair of NMR tubes (k). V h : Volume of released hemolymph. [X] h : Concentration of metabolite X in the hemolymph. I ′ 1 f : Intensity of 1 H resonance of the 13 C-formate standard when larvae do not have their cuticles ruptured (the “unopened” sample) in units relative the internal DSS concentration. I ′ 2 f : 1 H resonance of the standard when larvae have their cuticles ruptured (the “opened” sample).

    Journal: Journal of Proteome Research

    Article Title: An Improved Method for Measuring Absolute Metabolite Concentrations in Small Biofluid or Tissue Samples

    doi: 10.1021/acs.jproteome.8b00773

    Figure Lengend Snippet: The pVDTS workflow for liquid hemolymph samples. An accurately measured volume ( V f ) of saline containing the chosen standard (sodium 13 C-formate) at a known concentration [ f ] 0 is transferred to a number of larvae ( n ), with a collective hemolymph volume V h (a). An accurately measured volume of the droplet ( V t ) is removed and transferred into water in a microcentrifuge column (b)—this constitutes the origin of the control “unopened” sample. Larval cuticles are then ruptured to release hemolymph into the droplet (c), and a second accurately measured volume ( V t ) is removed from the droplet and transferred to water in a second microcentrifuge column (d). The following steps are then performed in parallel for the two samplings: microcentrifuge tubes are spun to remove debris and clear hemocytes from the “opened” sample (e). The cleared filtrates are then each transferred to a known volume ( V D ) of chloroform/methanol/water (green) containing a fixed concentration [ D ] 0 of DSS (f). After further separation of polar and nonpolar components via the Bligh–Dyer method (g), the upper aqueous phases containing polar species are aspirated to a second pair of microcentrifuge tubes (h). The solutions are evaporated to dryness (i) and the residues suspended in D 2 O (j) prior to transfer to a pair of NMR tubes (k). V h : Volume of released hemolymph. [X] h : Concentration of metabolite X in the hemolymph. I ′ 1 f : Intensity of 1 H resonance of the 13 C-formate standard when larvae do not have their cuticles ruptured (the “unopened” sample) in units relative the internal DSS concentration. I ′ 2 f : 1 H resonance of the standard when larvae have their cuticles ruptured (the “opened” sample).

    Article Snippet: For the “unopened” or “control” sample, after 1 min of contact with the larvae, 7.5 μL (V t ) of the sodium formate-saline solution was transferred with a Hamilton syringe to 100 μL deionized water in a 0.22 μm filter unit and spun into a microcentrifuge tube for 1 min at 13 000 rpm (Thermo Scientific, 75002425).

    Techniques: Concentration Assay, Nuclear Magnetic Resonance