microarray slides Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher superchip microarray slides
    Superchip Microarray Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superchip microarray slides/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    superchip microarray slides - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    89
    Agilent technologies microarrays slides
    Validation of <t>microarray</t> data by real-time qPCR . Mean (± SEM, n = 3) expression of the genes ef1alpha (housekeeping gene) , atoh1a , cdkn1b , JunB, gh1 , mlc1 , and myhc , as measured by microarray and qPCR. The expression profiles were similar between microarray and qPCR data.
    Microarrays Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarrays slides/product/Agilent technologies
    Average 89 stars, based on 624 article reviews
    Price from $9.99 to $1999.99
    microarrays slides - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray slide
    LGP2 regulates miRNA-mediated gene expression of endogenous genes in human cells. ( A ) XY plot of <t>microarray</t> signals of all transcripts expressed in WT and LGP2 −/− cells. ( B ) MA plots (above) and cumulative distributions (below) of all transcripts in LGP2 −/− cells normalized by their expression levels in WT cells. The predicted targets of miR-106b (left) and miR-19b-1 (right) are colored blue and pink, respectively. ZNFX1 and DERL2 are the predicted targets of miR-106b, but not miR-19b-1. ( C ) The differences of integral values of cumulative distribution between all transcripts and predicted targets were calculated on all of the predicted targets of each TRBP-bound pre-miRNA (sequence read enrichment by immunoprecipitation > 10) and TRBP-non-bound pre-miRNA (sequence read enrichment by immunoprecipitation
    Microarray Slide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slide/product/Agilent technologies
    Average 99 stars, based on 614 article reviews
    Price from $9.99 to $1999.99
    microarray slide - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    80
    MWG-Biotech slide microarrays
    LGP2 regulates miRNA-mediated gene expression of endogenous genes in human cells. ( A ) XY plot of <t>microarray</t> signals of all transcripts expressed in WT and LGP2 −/− cells. ( B ) MA plots (above) and cumulative distributions (below) of all transcripts in LGP2 −/− cells normalized by their expression levels in WT cells. The predicted targets of miR-106b (left) and miR-19b-1 (right) are colored blue and pink, respectively. ZNFX1 and DERL2 are the predicted targets of miR-106b, but not miR-19b-1. ( C ) The differences of integral values of cumulative distribution between all transcripts and predicted targets were calculated on all of the predicted targets of each TRBP-bound pre-miRNA (sequence read enrichment by immunoprecipitation > 10) and TRBP-non-bound pre-miRNA (sequence read enrichment by immunoprecipitation
    Slide Microarrays, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slide microarrays/product/MWG-Biotech
    Average 80 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    slide microarrays - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    88
    CombiMatrix microarray slide
    Venn diagrams showing numbers of barley genes shown by the <t>microarray</t> analysis to be significantly induced (A) or repressed (B) in barley embryos after inoculation with P. graminea for various combinations of genotypes and time‐points. The genotypes are Mirco‐ rdg2a (M‐ rdg2a ) and NIL3876‐ Rdg2a (NIL‐ Rdg2a ), and the time points are 7 and 14 dai. For each individual genotype/time‐point combination, the total number of induced/repressed genes is shown in parentheses.
    Microarray Slide, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slide/product/CombiMatrix
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    microarray slide - by Bioz Stars, 2020-03
    88/100 stars
      Buy from Supplier

    90
    Roche microarray slides
    Expression profiles of the gene pac1 and the homologues of palC and palF genes in the <t>microarray</t> data. A) Expression of the genes in cultures of the strain QM9414 at pH 3, 4.5 and 6. B) Expression of the genes in cultures of the Δ pac1 strain and in QM9414 at pH 6. Expression level of the genes is the normalised signal in the microarray data (not log2 transformed values), data on the three biological replicates is averaged. The time point and pH of the culture, as well as the strain, is indicated in the X axis labels. QM is an abbreviation for QM9414.
    Microarray Slides, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slides/product/Roche
    Average 90 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    microarray slides - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    90
    Corning Life Sciences microarray slides
    Volcano plot of M. sedula <t>microarray</t> dye-flip results (YE versus YE+FeSO 4 ). Negative x -axis values represent ORFs more highly transcribed on YE+FeSO 4 while positive x -axis values represent ORFs more highly transcribed on YE alone. As points
    Microarray Slides, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slides/product/Corning Life Sciences
    Average 90 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    microarray slides - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    89
    Full Moon BioSystems microarray slides
    Three types of spot morphology in a real <t>microarray</t> image . (A) The doughnut-shaped spot. (B) The peak-shaped spot. (C) The volcano-shaped spot.
    Microarray Slides, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slides/product/Full Moon BioSystems
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    microarray slides - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    89
    Genisphere microarray slide
    Three types of spot morphology in a real <t>microarray</t> image . (A) The doughnut-shaped spot. (B) The peak-shaped spot. (C) The volcano-shaped spot.
    Microarray Slide, supplied by Genisphere, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slide/product/Genisphere
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    microarray slide - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    89
    Kaneka Corp microarray slides
    Validation of the 9 genes signature by qRT-PCR on 5 new PTC and 5 new ATC. The <t>microarray</t> expressions is also represented. Log 2 ratios represent the expression ratios of the genes in the tumors versus a pool of 23 normal thyroid tissues.
    Microarray Slides, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 89/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slides/product/Kaneka Corp
    Average 89 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    microarray slides - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    99
    Thermo Fisher microarray slides
    Validation of the 10 differentially expressed genes obtained from <t>microarray</t> analysis by qRT-PCR, n = 3. The p -values of the qRT-PCR data are as follows: 0.0067 (C6ORF52), 0.3920 (CCDC84), 0.3661 (THYMOSIN), 0.8195 (PRVE), 0.3154 (HSPCB), 0.6534 (CYP2J2), 0.002 (AMPD3), 0.1622 (TOR1AIP2), 0.8329 (PTGES3), 0.8808 (ACOX3).
    Microarray Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slides/product/Thermo Fisher
    Average 99 stars, based on 422 article reviews
    Price from $9.99 to $1999.99
    microarray slides - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    91
    Corning Life Sciences microarray slide
    Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the <t>microarray.</t> The average fluorescence signal for each C. jejuni probe
    Microarray Slide, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slide/product/Corning Life Sciences
    Average 91 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    microarray slide - by Bioz Stars, 2020-03
    91/100 stars
      Buy from Supplier

    80
    Biodiscovery LLC microarray hybridizations microarray slides
    Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the <t>microarray.</t> The average fluorescence signal for each C. jejuni probe
    Microarray Hybridizations Microarray Slides, supplied by Biodiscovery LLC, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray hybridizations microarray slides/product/Biodiscovery LLC
    Average 80 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    microarray hybridizations microarray slides - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    83
    Agilent technologies 105k microarray slides
    Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the <t>microarray.</t> The average fluorescence signal for each C. jejuni probe
    105k Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/105k microarray slides/product/Agilent technologies
    Average 83 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    105k microarray slides - by Bioz Stars, 2020-03
    83/100 stars
      Buy from Supplier

    87
    Corning Life Sciences ultragap microarray slides
    Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the <t>microarray.</t> The average fluorescence signal for each C. jejuni probe
    Ultragap Microarray Slides, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 87/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultragap microarray slides/product/Corning Life Sciences
    Average 87 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    ultragap microarray slides - by Bioz Stars, 2020-03
    87/100 stars
      Buy from Supplier

    82
    SurModics codelink microarray slides
    Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the <t>microarray.</t> The average fluorescence signal for each C. jejuni probe
    Codelink Microarray Slides, supplied by SurModics, used in various techniques. Bioz Stars score: 82/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/codelink microarray slides/product/SurModics
    Average 82 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    codelink microarray slides - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    85
    Agilent technologies microarray gasket slide
    Transcriptomic response of S. pombe as a function of changes in iron levels and effect of php4 ∆ /php4 ∆ disruption relative to wild-type cells during meiotic differentiation. (A) Cultures of pat1-114/pat1-114 php4 + /php4 + cells were precultivated in the presence of Fe (0.74 µM) and Dip (50 µM) for 16 hr. Pretreated cells were further exposed to Fe (100 µM) and Dip (75 µM), respectively, and then immediately induced to undergo synchronous meiosis. After 7 hr of meiotic induction, total RNA was extracted and used for <t>microarray</t> experiments. The graph represents a genome-wide picture of differentially expressed genes (X axis) in iron-replete vs. iron-limited cells. For simplicity, only a few differentially expressed transcripts are labeled on the graph. (B) Genome-wide picture of differentially expressed genes in pat1-114/pat1-114 php4 Δ /php4 Δ vs. pat1-114/pat1-114 php4 + /php4 + cells that had been precultured (50 µM) and cultured (75 µM) in the presence of Dip. Genome-wide transcripts (X axis) were analyzed by DNA microarrays. All differentially expressed genes are depicted, although only a few of them are labeled on the graph for the sake of clarity. (C) Venn diagram representing the number of genes that were induced in iron-treated php4 + /php4 + cells compared to php4 + /php4 + cells grown under iron-limiting conditions and the number of genes that exhibited higher expression levels in iron-starved php4 ∆/ php4 ∆ compared to php4 + /php4 + cells grown under the same conditions. Dip, 2,2’-dipyridyl; Fe, iron(III) chloride.
    Microarray Gasket Slide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray gasket slide/product/Agilent technologies
    Average 85 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    microarray gasket slide - by Bioz Stars, 2020-03
    85/100 stars
      Buy from Supplier

    82
    OakLabs microarray slide hybridization
    Summary of <t>microarray</t> analysis of two different rice varieties, MTU9 (sensitive) and SML242 (tolerant) in response to B stress treatment. Data are from experiment Btox3. Differentially expressed genes (DEGs) are shown in red.
    Microarray Slide Hybridization, supplied by OakLabs, used in various techniques. Bioz Stars score: 82/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slide hybridization/product/OakLabs
    Average 82 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    microarray slide hybridization - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    77
    Kaneka Corp glass microarray slides
    Summary of <t>microarray</t> analysis of two different rice varieties, MTU9 (sensitive) and SML242 (tolerant) in response to B stress treatment. Data are from experiment Btox3. Differentially expressed genes (DEGs) are shown in red.
    Glass Microarray Slides, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glass microarray slides/product/Kaneka Corp
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    glass microarray slides - by Bioz Stars, 2020-03
    77/100 stars
      Buy from Supplier

    91
    Agilent technologies v19 microarray slides
    Summary of <t>microarray</t> analysis of two different rice varieties, MTU9 (sensitive) and SML242 (tolerant) in response to B stress treatment. Data are from experiment Btox3. Differentially expressed genes (DEGs) are shown in red.
    V19 Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v19 microarray slides/product/Agilent technologies
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    v19 microarray slides - by Bioz Stars, 2020-03
    91/100 stars
      Buy from Supplier

    89
    Agilent technologies v3 microarray slides
    Summary of <t>microarray</t> analysis of two different rice varieties, MTU9 (sensitive) and SML242 (tolerant) in response to B stress treatment. Data are from experiment Btox3. Differentially expressed genes (DEGs) are shown in red.
    V3 Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v3 microarray slides/product/Agilent technologies
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    v3 microarray slides - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    80
    Roche plex microarray slides
    Transcriptional profiling data comparing the production strategies. (A) Volcano plots of the combined triplicate RNA <t>microarray</t> data at the points of highest interest, 22 and 27 hours showing the spread of gene expression events in terms of up and down regulation between each production strategy comparison. (B) A table showing the transcriptional changes exceeding the 2-fold change threshold for each comparison and also listed by gene category throughout the cultivations. The total number of hits shows the combined number of transcriptional changes for all of the production strategies and the final column shows the mutual transcriptional changes observed between secretion and intracellular production. In the case of intracellular production most of these transcriptional changes observed are also found in the mutual category showing that many similar genes are highlighted between both types of production.
    Plex Microarray Slides, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plex microarray slides/product/Roche
    Average 80 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    plex microarray slides - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    86
    Corning Life Sciences microarray glass slides
    Experimental design. We used a total of four rats in the present <t>microarray</t> studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.
    Microarray Glass Slides, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray glass slides/product/Corning Life Sciences
    Average 86 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    microarray glass slides - by Bioz Stars, 2020-03
    86/100 stars
      Buy from Supplier

    89
    Agilent technologies 44k microarray slide
    Experimental design. We used a total of four rats in the present <t>microarray</t> studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.
    44k Microarray Slide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/44k microarray slide/product/Agilent technologies
    Average 89 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    44k microarray slide - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    94
    Agilent technologies 60k microarray slides
    Experimental design. We used a total of four rats in the present <t>microarray</t> studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.
    60k Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/60k microarray slides/product/Agilent technologies
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    60k microarray slides - by Bioz Stars, 2020-03
    94/100 stars
      Buy from Supplier

    81
    Millipore sigmascreen microarray slides
    Experimental design. We used a total of four rats in the present <t>microarray</t> studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.
    Sigmascreen Microarray Slides, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sigmascreen microarray slides/product/Millipore
    Average 81 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    sigmascreen microarray slides - by Bioz Stars, 2020-03
    81/100 stars
      Buy from Supplier

    94
    Thermo Fisher ultraclean microarray slides
    Experimental design. We used a total of four rats in the present <t>microarray</t> studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.
    Ultraclean Microarray Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultraclean microarray slides/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ultraclean microarray slides - by Bioz Stars, 2020-03
    94/100 stars
      Buy from Supplier

    90
    Millipore microarray slides
    Transcriptional profile of AI-2-treated cells versus the negative control determined by <t>microarray</t> analysis. Genes with negative z scores are upregulated in the AI-2-treated samples.
    Microarray Slides, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray slides/product/Millipore
    Average 90 stars, based on 149 article reviews
    Price from $9.99 to $1999.99
    microarray slides - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    77
    Toray microarray microarray slides
    Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent <t>microarray</t> chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .
    Microarray Microarray Slides, supplied by Toray, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray microarray slides/product/Toray
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microarray microarray slides - by Bioz Stars, 2020-03
    77/100 stars
      Buy from Supplier

    92
    US Biomax tissue microarray slide
    Correlation of TrkC with CRC pathogenesis and patient survival (A) Box-and-whisker (Tukey) plots of the mean expression of TrkC and NT-3 in CRC patients. TrkC and NT-3 levels were extracted from the Skrzypczak <t>microarray</t> dataset (GSE20916) and averaged in each tumor. Points below and above the whiskers are drawn as individual dots. P
    Tissue Microarray Slide, supplied by US Biomax, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissue microarray slide/product/US Biomax
    Average 92 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    tissue microarray slide - by Bioz Stars, 2020-03
    92/100 stars
      Buy from Supplier

    99
    US Biomax tissue microarray slides
    The correlation between RACK1 and Nanog expression in clinical HCC tissues. ( A ) 136 formaldehyde-fixed and paraffin-embedded clinical HCC samples were subjected to immunohistochemistry for RACK1 and Nanog staining on tissue <t>microarray</t> slides. Representative paired samples are shown. Scale bar: 50 μm. ( B ) Box plot of Nanog expression in HCC based on RACK1 expression in the tumors as revealed by immunohistochemistry. Kruskal-Wallis P =0.0009. ( C ) The protein levels of RACK1 positively correlated with those of Nanog in clinical HCC tissues as revealed by immunohistochemistry. Spearman r =0.2921, P =0.0006. ( D ) Fluorescent multiplex immunohistochemistry (mIHC) with tyramide signal amplification was performed on tissue microarray slides. Representative clinical HCC samples are shown. Scale bar: 400 μm.
    Tissue Microarray Slides, supplied by US Biomax, used in various techniques. Bioz Stars score: 99/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissue microarray slides/product/US Biomax
    Average 99 stars, based on 137 article reviews
    Price from $9.99 to $1999.99
    tissue microarray slides - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Validation of microarray data by real-time qPCR . Mean (± SEM, n = 3) expression of the genes ef1alpha (housekeeping gene) , atoh1a , cdkn1b , JunB, gh1 , mlc1 , and myhc , as measured by microarray and qPCR. The expression profiles were similar between microarray and qPCR data.

    Journal: BMC Neuroscience

    Article Title: Transcriptomic analysis of the zebrafish inner ear points to growth hormone mediated regeneration following acoustic trauma

    doi: 10.1186/1471-2202-12-88

    Figure Lengend Snippet: Validation of microarray data by real-time qPCR . Mean (± SEM, n = 3) expression of the genes ef1alpha (housekeeping gene) , atoh1a , cdkn1b , JunB, gh1 , mlc1 , and myhc , as measured by microarray and qPCR. The expression profiles were similar between microarray and qPCR data.

    Article Snippet: After hybridization, the microarray slides were washed with Agilent gene expression wash buffers.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Expressing

    Canonical pathway analysis reveals growth hormone-related gene regulation . Canonical pathway analysis showing known growth-hormone related pathways. Red-colored genes are up-regulated and gray-colored genes are down-regulated in the zebrafish microarray data set at two days following acoustic trauma. Numbers below genes represent fold changes and P -values.

    Journal: BMC Neuroscience

    Article Title: Transcriptomic analysis of the zebrafish inner ear points to growth hormone mediated regeneration following acoustic trauma

    doi: 10.1186/1471-2202-12-88

    Figure Lengend Snippet: Canonical pathway analysis reveals growth hormone-related gene regulation . Canonical pathway analysis showing known growth-hormone related pathways. Red-colored genes are up-regulated and gray-colored genes are down-regulated in the zebrafish microarray data set at two days following acoustic trauma. Numbers below genes represent fold changes and P -values.

    Article Snippet: After hybridization, the microarray slides were washed with Agilent gene expression wash buffers.

    Techniques: Microarray

    Volcano plots and heat maps showing the time course of acoustic trauma-related differential gene expression in the zebrafish inner ear . RNA samples from zebrafish inner ears of controls or subjects exposed to acoustic trauma and allowed to recover for either two or four days and then hybridized to a zebrafish microarray, which measures expression (mRNA abundance) for 21,000 gene transcipts. Volcano plots show differential gene expression between (A) controls and two days post-trauma (D2), (B) controls and four days post-trauma (D4), and (C) two versus four days post-trauma. The Y-axes of the volcano plots display the negative log (base 10) of P values from paired t -tests, while the X-axes show the log (base 2) of the fold differences between the groups. The horizontal line represents the P = 0.05 value, and the vertical lines corresponding to fold differences of 1.4 and -1.4, respectively. Genes with paired t-test P

    Journal: BMC Neuroscience

    Article Title: Transcriptomic analysis of the zebrafish inner ear points to growth hormone mediated regeneration following acoustic trauma

    doi: 10.1186/1471-2202-12-88

    Figure Lengend Snippet: Volcano plots and heat maps showing the time course of acoustic trauma-related differential gene expression in the zebrafish inner ear . RNA samples from zebrafish inner ears of controls or subjects exposed to acoustic trauma and allowed to recover for either two or four days and then hybridized to a zebrafish microarray, which measures expression (mRNA abundance) for 21,000 gene transcipts. Volcano plots show differential gene expression between (A) controls and two days post-trauma (D2), (B) controls and four days post-trauma (D4), and (C) two versus four days post-trauma. The Y-axes of the volcano plots display the negative log (base 10) of P values from paired t -tests, while the X-axes show the log (base 2) of the fold differences between the groups. The horizontal line represents the P = 0.05 value, and the vertical lines corresponding to fold differences of 1.4 and -1.4, respectively. Genes with paired t-test P

    Article Snippet: After hybridization, the microarray slides were washed with Agilent gene expression wash buffers.

    Techniques: Expressing, Microarray

    Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent microarray chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent microarray chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .

    Article Snippet: (1) Agilent microarray Microarray slides were scanned using an Agilent microarray scanner G2505B (Agilent technology) and microarray images were automatically analyzed using Feature extraction™ software, version 9.5.1.1 (Agilent technology).

    Techniques: Expressing, Microarray

    MicroRNA expression level in human liver and prostate tissues. For the microarray platforms, log2 transformed values of representative signal intensity for detection call-positive microRNAs were plotted. For the Taqman analysis, Ct values of microRNAs were plotted. Red and blue lines indicate Y = X line and regressed linear line, respectively. In all scatter plots. blue lines were shifted upward, which indicated that the general microRNA expression level in human prostate was higher than in human liver.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: MicroRNA expression level in human liver and prostate tissues. For the microarray platforms, log2 transformed values of representative signal intensity for detection call-positive microRNAs were plotted. For the Taqman analysis, Ct values of microRNAs were plotted. Red and blue lines indicate Y = X line and regressed linear line, respectively. In all scatter plots. blue lines were shifted upward, which indicated that the general microRNA expression level in human prostate was higher than in human liver.

    Article Snippet: (1) Agilent microarray Microarray slides were scanned using an Agilent microarray scanner G2505B (Agilent technology) and microarray images were automatically analyzed using Feature extraction™ software, version 9.5.1.1 (Agilent technology).

    Techniques: Expressing, Microarray, Transformation Assay

    Correlation between microarray and Taqman data. The scatter plots compare the log-ratio differential expression values from each microarray platform relative to values obtained by the Taqman assays. Each point represents a microRNA that was measured on both the microarray and Taqman assays. Only microRNAs that were generally detected in both human liver and prostate were used in the comparisons. Among 142 total microRNAs assayed by the Taqman system, the number of microRNAs analyzed for correlation to the Taqman assays are listed in the table. The red and blue lines shown are the ideal Y = X line, and the regressed line from the scatter plots, respectively. Spearman's correlation coefficients (Rs), slope and Y intercept of regressed line were shown in the table.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Correlation between microarray and Taqman data. The scatter plots compare the log-ratio differential expression values from each microarray platform relative to values obtained by the Taqman assays. Each point represents a microRNA that was measured on both the microarray and Taqman assays. Only microRNAs that were generally detected in both human liver and prostate were used in the comparisons. Among 142 total microRNAs assayed by the Taqman system, the number of microRNAs analyzed for correlation to the Taqman assays are listed in the table. The red and blue lines shown are the ideal Y = X line, and the regressed line from the scatter plots, respectively. Spearman's correlation coefficients (Rs), slope and Y intercept of regressed line were shown in the table.

    Article Snippet: (1) Agilent microarray Microarray slides were scanned using an Agilent microarray scanner G2505B (Agilent technology) and microarray images were automatically analyzed using Feature extraction™ software, version 9.5.1.1 (Agilent technology).

    Techniques: Microarray, Expressing

    Agreement of microRNA list between microarray platforms and the Taqman assay. MicroRNAs that were listed, or not listed in both microarray and the Taqman assay, were considered as true positive (TP) or true negative (TN), respectively. MicroRNAs that were listed in either microarray or Taqman assays, were handled as false positive (FP) or false negative (FN), respectively. 7A and B: These graphs indicated the concordance of detection call between microarray platforms and the Taqman assay, using 142 microRNAs assayed by both microarray and the Taqman system. 7C: This bar graph demonstrated the concordance of microRNAs identified as differentially expressed between microarray platforms and the Taqman assay system.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Agreement of microRNA list between microarray platforms and the Taqman assay. MicroRNAs that were listed, or not listed in both microarray and the Taqman assay, were considered as true positive (TP) or true negative (TN), respectively. MicroRNAs that were listed in either microarray or Taqman assays, were handled as false positive (FP) or false negative (FN), respectively. 7A and B: These graphs indicated the concordance of detection call between microarray platforms and the Taqman assay, using 142 microRNAs assayed by both microarray and the Taqman system. 7C: This bar graph demonstrated the concordance of microRNAs identified as differentially expressed between microarray platforms and the Taqman assay system.

    Article Snippet: (1) Agilent microarray Microarray slides were scanned using an Agilent microarray scanner G2505B (Agilent technology) and microarray images were automatically analyzed using Feature extraction™ software, version 9.5.1.1 (Agilent technology).

    Techniques: Microarray, TaqMan Assay

    LGP2 regulates miRNA-mediated gene expression of endogenous genes in human cells. ( A ) XY plot of microarray signals of all transcripts expressed in WT and LGP2 −/− cells. ( B ) MA plots (above) and cumulative distributions (below) of all transcripts in LGP2 −/− cells normalized by their expression levels in WT cells. The predicted targets of miR-106b (left) and miR-19b-1 (right) are colored blue and pink, respectively. ZNFX1 and DERL2 are the predicted targets of miR-106b, but not miR-19b-1. ( C ) The differences of integral values of cumulative distribution between all transcripts and predicted targets were calculated on all of the predicted targets of each TRBP-bound pre-miRNA (sequence read enrichment by immunoprecipitation > 10) and TRBP-non-bound pre-miRNA (sequence read enrichment by immunoprecipitation

    Journal: Nucleic Acids Research

    Article Title: LGP2 virus sensor regulates gene expression network mediated by TRBP-bound microRNAs

    doi: 10.1093/nar/gky575

    Figure Lengend Snippet: LGP2 regulates miRNA-mediated gene expression of endogenous genes in human cells. ( A ) XY plot of microarray signals of all transcripts expressed in WT and LGP2 −/− cells. ( B ) MA plots (above) and cumulative distributions (below) of all transcripts in LGP2 −/− cells normalized by their expression levels in WT cells. The predicted targets of miR-106b (left) and miR-19b-1 (right) are colored blue and pink, respectively. ZNFX1 and DERL2 are the predicted targets of miR-106b, but not miR-19b-1. ( C ) The differences of integral values of cumulative distribution between all transcripts and predicted targets were calculated on all of the predicted targets of each TRBP-bound pre-miRNA (sequence read enrichment by immunoprecipitation > 10) and TRBP-non-bound pre-miRNA (sequence read enrichment by immunoprecipitation

    Article Snippet: Cy-3 labeled RNAs were fragmented by Gene Expression Hybridization kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, microarray slide was scanned by DNA Microarray Scanner (Agilent Technologies) and quantified signals by Feature Extraction 10.5.1.1 (Agilent Technologies).

    Techniques: Expressing, Microarray, Sequencing, Immunoprecipitation

    Venn diagrams showing numbers of barley genes shown by the microarray analysis to be significantly induced (A) or repressed (B) in barley embryos after inoculation with P. graminea for various combinations of genotypes and time‐points. The genotypes are Mirco‐ rdg2a (M‐ rdg2a ) and NIL3876‐ Rdg2a (NIL‐ Rdg2a ), and the time points are 7 and 14 dai. For each individual genotype/time‐point combination, the total number of induced/repressed genes is shown in parentheses.

    Journal: Molecular Plant Pathology

    Article Title: Histological and molecular analysis of Rdg2a barley resistance to leaf stripe

    doi: 10.1111/j.1364-3703.2008.00479.x

    Figure Lengend Snippet: Venn diagrams showing numbers of barley genes shown by the microarray analysis to be significantly induced (A) or repressed (B) in barley embryos after inoculation with P. graminea for various combinations of genotypes and time‐points. The genotypes are Mirco‐ rdg2a (M‐ rdg2a ) and NIL3876‐ Rdg2a (NIL‐ Rdg2a ), and the time points are 7 and 14 dai. For each individual genotype/time‐point combination, the total number of induced/repressed genes is shown in parentheses.

    Article Snippet: A total of 2240 35–40 mer probes were synthesized onto each sector of a CustomArray 4 × 2000 microarray slide (CombiMatrix Corp., WA).

    Techniques: Microarray

    Expression profiles of the gene pac1 and the homologues of palC and palF genes in the microarray data. A) Expression of the genes in cultures of the strain QM9414 at pH 3, 4.5 and 6. B) Expression of the genes in cultures of the Δ pac1 strain and in QM9414 at pH 6. Expression level of the genes is the normalised signal in the microarray data (not log2 transformed values), data on the three biological replicates is averaged. The time point and pH of the culture, as well as the strain, is indicated in the X axis labels. QM is an abbreviation for QM9414.

    Journal: Microbial Cell Factories

    Article Title: The effects of extracellular pH and of the transcriptional regulator PACI on the transcriptome of Trichoderma reesei

    doi: 10.1186/s12934-015-0247-z

    Figure Lengend Snippet: Expression profiles of the gene pac1 and the homologues of palC and palF genes in the microarray data. A) Expression of the genes in cultures of the strain QM9414 at pH 3, 4.5 and 6. B) Expression of the genes in cultures of the Δ pac1 strain and in QM9414 at pH 6. Expression level of the genes is the normalised signal in the microarray data (not log2 transformed values), data on the three biological replicates is averaged. The time point and pH of the culture, as well as the strain, is indicated in the X axis labels. QM is an abbreviation for QM9414.

    Article Snippet: The probe design and manufacturing of the microarray slides was carried out by RocheNimbleGen using an array design based on the T. reesei genome version 2.0 [ ].

    Techniques: Expressing, Microarray, Transformation Assay

    Volcano plot of M. sedula microarray dye-flip results (YE versus YE+FeSO 4 ). Negative x -axis values represent ORFs more highly transcribed on YE+FeSO 4 while positive x -axis values represent ORFs more highly transcribed on YE alone. As points

    Journal: Applied and Environmental Microbiology

    Article Title: The Genome Sequence of the Metal-Mobilizing, Extremely Thermoacidophilic Archaeon Metallosphaera sedula Provides Insights into Bioleaching-Associated Metabolism ▿ Provides Insights into Bioleaching-Associated Metabolism ▿ †

    doi: 10.1128/AEM.02019-07

    Figure Lengend Snippet: Volcano plot of M. sedula microarray dye-flip results (YE versus YE+FeSO 4 ). Negative x -axis values represent ORFs more highly transcribed on YE+FeSO 4 while positive x -axis values represent ORFs more highly transcribed on YE alone. As points

    Article Snippet: RNA was extracted and purified (RNAqueous; Ambion), reverse transcribed (Superscript III, Invitrogen), repurified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to one of two microarray slides (Corning).

    Techniques: Microarray

    Three types of spot morphology in a real microarray image . (A) The doughnut-shaped spot. (B) The peak-shaped spot. (C) The volcano-shaped spot.

    Journal: BMC Bioinformatics

    Article Title: Characterization and simulation of cDNA microarray spots using a novel mathematical model

    doi: 10.1186/1471-2105-8-485

    Figure Lengend Snippet: Three types of spot morphology in a real microarray image . (A) The doughnut-shaped spot. (B) The peak-shaped spot. (C) The volcano-shaped spot.

    Article Snippet: While homemade poly-L-lysine-coated glass slides have been widely used, several commercial microarray slides are now preferred, such as the FMB cDNA slide (Full Moon Biosystems Inc.), ArrayIt SuperAmine and SuperAldehyde slides (TeleChem International, Inc.).

    Techniques: Microarray

    Various spot shapes in real microarray images . (A) Real microarray images contain spots in various shapes [38] [40]. (B) The 3D image of spots in the real microarray images were reconstructed by plotting image intensity on the z-axis (C) The radial profiles of the 3D images were obtained where the section passed through the centre.

    Journal: BMC Bioinformatics

    Article Title: Characterization and simulation of cDNA microarray spots using a novel mathematical model

    doi: 10.1186/1471-2105-8-485

    Figure Lengend Snippet: Various spot shapes in real microarray images . (A) Real microarray images contain spots in various shapes [38] [40]. (B) The 3D image of spots in the real microarray images were reconstructed by plotting image intensity on the z-axis (C) The radial profiles of the 3D images were obtained where the section passed through the centre.

    Article Snippet: While homemade poly-L-lysine-coated glass slides have been widely used, several commercial microarray slides are now preferred, such as the FMB cDNA slide (Full Moon Biosystems Inc.), ArrayIt SuperAmine and SuperAldehyde slides (TeleChem International, Inc.).

    Techniques: Microarray

    Validation of the 9 genes signature by qRT-PCR on 5 new PTC and 5 new ATC. The microarray expressions is also represented. Log 2 ratios represent the expression ratios of the genes in the tumors versus a pool of 23 normal thyroid tissues.

    Journal: PLoS ONE

    Article Title: mRNA Expression in Papillary and Anaplastic Thyroid Carcinoma: Molecular Anatomy of a Killing Switch

    doi: 10.1371/journal.pone.0037807

    Figure Lengend Snippet: Validation of the 9 genes signature by qRT-PCR on 5 new PTC and 5 new ATC. The microarray expressions is also represented. Log 2 ratios represent the expression ratios of the genes in the tumors versus a pool of 23 normal thyroid tissues.

    Article Snippet: Validation of Microarray Results by Quantitative Real-time RT-PCR (qRT-PCR) A number of modulated genes on the microarray slides were validated using qRT-PCR (SYBR Green, Eurogentec, Liege, Belgium).

    Techniques: Quantitative RT-PCR, Microarray, Expressing

    Validation of the 10 differentially expressed genes obtained from microarray analysis by qRT-PCR, n = 3. The p -values of the qRT-PCR data are as follows: 0.0067 (C6ORF52), 0.3920 (CCDC84), 0.3661 (THYMOSIN), 0.8195 (PRVE), 0.3154 (HSPCB), 0.6534 (CYP2J2), 0.002 (AMPD3), 0.1622 (TOR1AIP2), 0.8329 (PTGES3), 0.8808 (ACOX3).

    Journal: PLoS ONE

    Article Title: Genome-Wide Gene Expression Profiles in Lung Tissues of Pig Breeds Differing in Resistance to Porcine Reproductive and Respiratory Syndrome Virus

    doi: 10.1371/journal.pone.0086101

    Figure Lengend Snippet: Validation of the 10 differentially expressed genes obtained from microarray analysis by qRT-PCR, n = 3. The p -values of the qRT-PCR data are as follows: 0.0067 (C6ORF52), 0.3920 (CCDC84), 0.3661 (THYMOSIN), 0.8195 (PRVE), 0.3154 (HSPCB), 0.6534 (CYP2J2), 0.002 (AMPD3), 0.1622 (TOR1AIP2), 0.8329 (PTGES3), 0.8808 (ACOX3).

    Article Snippet: After hybridization, the microarray slides were washed and dyed with the Affymetrix Fluidics Station 450.

    Techniques: Microarray, Quantitative RT-PCR

    Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent microarray chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent microarray chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .

    Article Snippet: (4) Invitrogen microarray Microarray slides were scanned using Agilent microarray scanner G2505B and the microarray image was analyzed using the Genepix Pro™ 4.0 software.

    Techniques: Expressing, Microarray

    MicroRNA expression level in human liver and prostate tissues. For the microarray platforms, log2 transformed values of representative signal intensity for detection call-positive microRNAs were plotted. For the Taqman analysis, Ct values of microRNAs were plotted. Red and blue lines indicate Y = X line and regressed linear line, respectively. In all scatter plots. blue lines were shifted upward, which indicated that the general microRNA expression level in human prostate was higher than in human liver.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: MicroRNA expression level in human liver and prostate tissues. For the microarray platforms, log2 transformed values of representative signal intensity for detection call-positive microRNAs were plotted. For the Taqman analysis, Ct values of microRNAs were plotted. Red and blue lines indicate Y = X line and regressed linear line, respectively. In all scatter plots. blue lines were shifted upward, which indicated that the general microRNA expression level in human prostate was higher than in human liver.

    Article Snippet: (4) Invitrogen microarray Microarray slides were scanned using Agilent microarray scanner G2505B and the microarray image was analyzed using the Genepix Pro™ 4.0 software.

    Techniques: Expressing, Microarray, Transformation Assay

    Correlation between microarray and Taqman data. The scatter plots compare the log-ratio differential expression values from each microarray platform relative to values obtained by the Taqman assays. Each point represents a microRNA that was measured on both the microarray and Taqman assays. Only microRNAs that were generally detected in both human liver and prostate were used in the comparisons. Among 142 total microRNAs assayed by the Taqman system, the number of microRNAs analyzed for correlation to the Taqman assays are listed in the table. The red and blue lines shown are the ideal Y = X line, and the regressed line from the scatter plots, respectively. Spearman's correlation coefficients (Rs), slope and Y intercept of regressed line were shown in the table.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Correlation between microarray and Taqman data. The scatter plots compare the log-ratio differential expression values from each microarray platform relative to values obtained by the Taqman assays. Each point represents a microRNA that was measured on both the microarray and Taqman assays. Only microRNAs that were generally detected in both human liver and prostate were used in the comparisons. Among 142 total microRNAs assayed by the Taqman system, the number of microRNAs analyzed for correlation to the Taqman assays are listed in the table. The red and blue lines shown are the ideal Y = X line, and the regressed line from the scatter plots, respectively. Spearman's correlation coefficients (Rs), slope and Y intercept of regressed line were shown in the table.

    Article Snippet: (4) Invitrogen microarray Microarray slides were scanned using Agilent microarray scanner G2505B and the microarray image was analyzed using the Genepix Pro™ 4.0 software.

    Techniques: Microarray, Expressing

    Agreement of microRNA list between microarray platforms and the Taqman assay. MicroRNAs that were listed, or not listed in both microarray and the Taqman assay, were considered as true positive (TP) or true negative (TN), respectively. MicroRNAs that were listed in either microarray or Taqman assays, were handled as false positive (FP) or false negative (FN), respectively. 7A and B: These graphs indicated the concordance of detection call between microarray platforms and the Taqman assay, using 142 microRNAs assayed by both microarray and the Taqman system. 7C: This bar graph demonstrated the concordance of microRNAs identified as differentially expressed between microarray platforms and the Taqman assay system.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Agreement of microRNA list between microarray platforms and the Taqman assay. MicroRNAs that were listed, or not listed in both microarray and the Taqman assay, were considered as true positive (TP) or true negative (TN), respectively. MicroRNAs that were listed in either microarray or Taqman assays, were handled as false positive (FP) or false negative (FN), respectively. 7A and B: These graphs indicated the concordance of detection call between microarray platforms and the Taqman assay, using 142 microRNAs assayed by both microarray and the Taqman system. 7C: This bar graph demonstrated the concordance of microRNAs identified as differentially expressed between microarray platforms and the Taqman assay system.

    Article Snippet: (4) Invitrogen microarray Microarray slides were scanned using Agilent microarray scanner G2505B and the microarray image was analyzed using the Genepix Pro™ 4.0 software.

    Techniques: Microarray, TaqMan Assay

    ELP expression in the tammar mammary gland throughout the reproductive cycle. A. Northern analysis of total RNA (10 μg) extracted from the mammary glands of tammar wallabies during pregnancy (P, Phase 1) and lactation (L, Phase 2A, Phase 2B and Phase 3), from non-sucked (NS) glands and from a virgin female (~220 days of age). Tammar ELP expression was undetected in the virgin gland, minimal during pregnancy (Phase 1) and then induced at parturition and expressed during early lactation (Phase 2A). ELP was down-regulated at mid-lactation (Phase 2B), consistent with [ 13 , 20 , 21 ]. ELP transcripts were not detected in Phase 3. ELP expression also declined postpartum in non-sucked glands. Tammar LGB was used as a positive control for lactation and exhibited a similar expression pattern to ELP , but with LGB expression increased (but not significantly so) during Phases 2B and 3, as reported previously [ 13 , 68 , 69 ]. Ribosomal RNA bands indicate RNA integrity and loading. B. Microarray analysis of the tammar mammary gland [ArrayExpress: E-MTAB-1057] supported the quantitative analysis of Northern blot (data not shown) and microarray data reported by [ 69 ]. Expression of the ELP and LGB milk protein genes and the housekeeping gene GAPDH (glyceraldehyde 3-phosphate dehydrogenase) is depicted as average normalised raw intensity based upon the expression n = 3, 7 and 2 clones on each microarray respectively ± SEM (Additional file 12: Table S5). Whilst ELP (red) and LGB (blue) expression differed during the reproductive cycle, GAPDH (green) expression was constant.

    Journal: BMC Evolutionary Biology

    Article Title: The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene

    doi: 10.1186/1471-2148-12-80

    Figure Lengend Snippet: ELP expression in the tammar mammary gland throughout the reproductive cycle. A. Northern analysis of total RNA (10 μg) extracted from the mammary glands of tammar wallabies during pregnancy (P, Phase 1) and lactation (L, Phase 2A, Phase 2B and Phase 3), from non-sucked (NS) glands and from a virgin female (~220 days of age). Tammar ELP expression was undetected in the virgin gland, minimal during pregnancy (Phase 1) and then induced at parturition and expressed during early lactation (Phase 2A). ELP was down-regulated at mid-lactation (Phase 2B), consistent with [ 13 , 20 , 21 ]. ELP transcripts were not detected in Phase 3. ELP expression also declined postpartum in non-sucked glands. Tammar LGB was used as a positive control for lactation and exhibited a similar expression pattern to ELP , but with LGB expression increased (but not significantly so) during Phases 2B and 3, as reported previously [ 13 , 68 , 69 ]. Ribosomal RNA bands indicate RNA integrity and loading. B. Microarray analysis of the tammar mammary gland [ArrayExpress: E-MTAB-1057] supported the quantitative analysis of Northern blot (data not shown) and microarray data reported by [ 69 ]. Expression of the ELP and LGB milk protein genes and the housekeeping gene GAPDH (glyceraldehyde 3-phosphate dehydrogenase) is depicted as average normalised raw intensity based upon the expression n = 3, 7 and 2 clones on each microarray respectively ± SEM (Additional file 12: Table S5). Whilst ELP (red) and LGB (blue) expression differed during the reproductive cycle, GAPDH (green) expression was constant.

    Article Snippet: Glass microarray slides were printed by the Peter MacCallum Cancer Centre Microarray Core Facility, Melbourne, Australia and contained 10,368 tammar cDNA spots which were derived from a commercially prepared (Life Technologies, Rockville, MD, USA), normalised 15,001 tammar mammary gland EST (expressed sequence tag) library.

    Techniques: Expressing, Northern Blot, Positive Control, Microarray, Clone Assay

    Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the microarray. The average fluorescence signal for each C. jejuni probe

    Journal: Applied and Environmental Microbiology

    Article Title: Detection and Genotyping of Arcobacter and Campylobacter Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ †

    doi: 10.1128/AEM.02984-06

    Figure Lengend Snippet: Threshold sensitivity of the DNA oligonucleotide array. Genomic DNA from various concentrations of C. jejuni strain NCTC11168 cells were isolated, CyDye labeled, and hybridized to the microarray. The average fluorescence signal for each C. jejuni probe

    Article Snippet: The microarray slide was placed in a hybridization chamber (Corning) and was incubated at 42°C for 18 h, as in previous studies ( ).

    Techniques: Isolation, Labeling, Microarray, Fluorescence

    Validation of the DNA oligonucleotide array for identifying pathogens in food. (A) Genomic DNA from bacterial isolates recovered from a 24-h enrichment broth was labeled with CyDye and hybridized to the microarray. The average fluorescence signal for

    Journal: Applied and Environmental Microbiology

    Article Title: Detection and Genotyping of Arcobacter and Campylobacter Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ †

    doi: 10.1128/AEM.02984-06

    Figure Lengend Snippet: Validation of the DNA oligonucleotide array for identifying pathogens in food. (A) Genomic DNA from bacterial isolates recovered from a 24-h enrichment broth was labeled with CyDye and hybridized to the microarray. The average fluorescence signal for

    Article Snippet: The microarray slide was placed in a hybridization chamber (Corning) and was incubated at 42°C for 18 h, as in previous studies ( ).

    Techniques: Labeling, Microarray, Fluorescence

    Probe specificity of the DNA oligonucleotide array. Genomic DNA from A. butzleri ( Ab ), A. cryaerophilus ( Ac ), C. coli ( Cc ), and C. jejuni ( Cj ) reference strains (Table ) was labeled separately with CyDye and hybridized to the microarray.

    Journal: Applied and Environmental Microbiology

    Article Title: Detection and Genotyping of Arcobacter and Campylobacter Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ †

    doi: 10.1128/AEM.02984-06

    Figure Lengend Snippet: Probe specificity of the DNA oligonucleotide array. Genomic DNA from A. butzleri ( Ab ), A. cryaerophilus ( Ac ), C. coli ( Cc ), and C. jejuni ( Cj ) reference strains (Table ) was labeled separately with CyDye and hybridized to the microarray.

    Article Snippet: The microarray slide was placed in a hybridization chamber (Corning) and was incubated at 42°C for 18 h, as in previous studies ( ).

    Techniques: Labeling, Microarray

    Multiplex PCR for species identification of Arcobacter ) was performed to validate the microarray data for species identification of Arcobacter isolates from retail chicken samples as determined by microarray analysis.

    Journal: Applied and Environmental Microbiology

    Article Title: Detection and Genotyping of Arcobacter and Campylobacter Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ Isolates from Retail Chicken Samples by Use of DNA Oligonucleotide Arrays ▿ †

    doi: 10.1128/AEM.02984-06

    Figure Lengend Snippet: Multiplex PCR for species identification of Arcobacter ) was performed to validate the microarray data for species identification of Arcobacter isolates from retail chicken samples as determined by microarray analysis.

    Article Snippet: The microarray slide was placed in a hybridization chamber (Corning) and was incubated at 42°C for 18 h, as in previous studies ( ).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Microarray

    Transcriptomic response of S. pombe as a function of changes in iron levels and effect of php4 ∆ /php4 ∆ disruption relative to wild-type cells during meiotic differentiation. (A) Cultures of pat1-114/pat1-114 php4 + /php4 + cells were precultivated in the presence of Fe (0.74 µM) and Dip (50 µM) for 16 hr. Pretreated cells were further exposed to Fe (100 µM) and Dip (75 µM), respectively, and then immediately induced to undergo synchronous meiosis. After 7 hr of meiotic induction, total RNA was extracted and used for microarray experiments. The graph represents a genome-wide picture of differentially expressed genes (X axis) in iron-replete vs. iron-limited cells. For simplicity, only a few differentially expressed transcripts are labeled on the graph. (B) Genome-wide picture of differentially expressed genes in pat1-114/pat1-114 php4 Δ /php4 Δ vs. pat1-114/pat1-114 php4 + /php4 + cells that had been precultured (50 µM) and cultured (75 µM) in the presence of Dip. Genome-wide transcripts (X axis) were analyzed by DNA microarrays. All differentially expressed genes are depicted, although only a few of them are labeled on the graph for the sake of clarity. (C) Venn diagram representing the number of genes that were induced in iron-treated php4 + /php4 + cells compared to php4 + /php4 + cells grown under iron-limiting conditions and the number of genes that exhibited higher expression levels in iron-starved php4 ∆/ php4 ∆ compared to php4 + /php4 + cells grown under the same conditions. Dip, 2,2’-dipyridyl; Fe, iron(III) chloride.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells

    doi: 10.1534/g3.116.031898

    Figure Lengend Snippet: Transcriptomic response of S. pombe as a function of changes in iron levels and effect of php4 ∆ /php4 ∆ disruption relative to wild-type cells during meiotic differentiation. (A) Cultures of pat1-114/pat1-114 php4 + /php4 + cells were precultivated in the presence of Fe (0.74 µM) and Dip (50 µM) for 16 hr. Pretreated cells were further exposed to Fe (100 µM) and Dip (75 µM), respectively, and then immediately induced to undergo synchronous meiosis. After 7 hr of meiotic induction, total RNA was extracted and used for microarray experiments. The graph represents a genome-wide picture of differentially expressed genes (X axis) in iron-replete vs. iron-limited cells. For simplicity, only a few differentially expressed transcripts are labeled on the graph. (B) Genome-wide picture of differentially expressed genes in pat1-114/pat1-114 php4 Δ /php4 Δ vs. pat1-114/pat1-114 php4 + /php4 + cells that had been precultured (50 µM) and cultured (75 µM) in the presence of Dip. Genome-wide transcripts (X axis) were analyzed by DNA microarrays. All differentially expressed genes are depicted, although only a few of them are labeled on the graph for the sake of clarity. (C) Venn diagram representing the number of genes that were induced in iron-treated php4 + /php4 + cells compared to php4 + /php4 + cells grown under iron-limiting conditions and the number of genes that exhibited higher expression levels in iron-starved php4 ∆/ php4 ∆ compared to php4 + /php4 + cells grown under the same conditions. Dip, 2,2’-dipyridyl; Fe, iron(III) chloride.

    Article Snippet: First, the microarray gasket slide from Agilent Technologies was improved in that it contained a larger number of probes (15,000) which allowed a ∼2–3 × increased coverage for each S. pombe ORF.

    Techniques: Microarray, Genome Wide, Labeling, Cell Culture, Expressing

    Summary of microarray analysis of two different rice varieties, MTU9 (sensitive) and SML242 (tolerant) in response to B stress treatment. Data are from experiment Btox3. Differentially expressed genes (DEGs) are shown in red.

    Journal: Journal of Experimental Botany

    Article Title: Genetic factors underlying boron toxicity tolerance in rice: genome-wide association study and transcriptomic analysis

    doi: 10.1093/jxb/erw423

    Figure Lengend Snippet: Summary of microarray analysis of two different rice varieties, MTU9 (sensitive) and SML242 (tolerant) in response to B stress treatment. Data are from experiment Btox3. Differentially expressed genes (DEGs) are shown in red.

    Article Snippet: Only samples with RNA integrity number > 8 were used for microarray slide hybridization (OakLabs GmbH, Hennigsdorf, Germany).

    Techniques: Microarray

    Transcriptional profiling data comparing the production strategies. (A) Volcano plots of the combined triplicate RNA microarray data at the points of highest interest, 22 and 27 hours showing the spread of gene expression events in terms of up and down regulation between each production strategy comparison. (B) A table showing the transcriptional changes exceeding the 2-fold change threshold for each comparison and also listed by gene category throughout the cultivations. The total number of hits shows the combined number of transcriptional changes for all of the production strategies and the final column shows the mutual transcriptional changes observed between secretion and intracellular production. In the case of intracellular production most of these transcriptional changes observed are also found in the mutual category showing that many similar genes are highlighted between both types of production.

    Journal: BMC Biotechnology

    Article Title: Comparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei

    doi: 10.1186/s12896-014-0091-y

    Figure Lengend Snippet: Transcriptional profiling data comparing the production strategies. (A) Volcano plots of the combined triplicate RNA microarray data at the points of highest interest, 22 and 27 hours showing the spread of gene expression events in terms of up and down regulation between each production strategy comparison. (B) A table showing the transcriptional changes exceeding the 2-fold change threshold for each comparison and also listed by gene category throughout the cultivations. The total number of hits shows the combined number of transcriptional changes for all of the production strategies and the final column shows the mutual transcriptional changes observed between secretion and intracellular production. In the case of intracellular production most of these transcriptional changes observed are also found in the mutual category showing that many similar genes are highlighted between both types of production.

    Article Snippet: The labelled cDNA was hybridised on an automated HS12 system (Roche) to custom-made four plex microarray slides (Roche) designed from the T. reesei genome version 2.0 [ , ].

    Techniques: Microarray, Expressing

    Experimental design. We used a total of four rats in the present microarray studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.

    Journal: Genome Biology

    Article Title: Systems analysis of circadian time-dependent neuronal epidermal growth factor receptor signaling

    doi: 10.1186/gb-2006-7-6-r48

    Figure Lengend Snippet: Experimental design. We used a total of four rats in the present microarray studies, two for the circadian day (8 hours after lights on, rats (a) and (b)), and two for the circadian night (2 hours after lights off, rats (c) and (d)). From each rat we obtained coronal slices that contained two SCN (left and right), separated by the third ventricle. Slices were separated along the third ventricle and placed in media containing EGF (20 nM) or control vehicle (C) for one hour. RNA for use with the microarrays was then extracted from SCN punches from the slices.

    Article Snippet: Microarrays In-house constructed cDNA microarrays containing 2,700 unique University of Iowa Rat clones from all rat tissues spotted at least twice per slide (5,464 spots split across 48 subarrays per slide) onto 1' by 3' glass microarray slides (Corning, Corning, NY, USA) were used in the present study.

    Techniques: Microarray

    Transcriptional profile of AI-2-treated cells versus the negative control determined by microarray analysis. Genes with negative z scores are upregulated in the AI-2-treated samples.

    Journal:

    Article Title: Autoinducer-2 Triggers the Oxidative Stress Response in Mycobacterium avium, Leading to Biofilm Formation ▿, Leading to Biofilm Formation ▿ †

    doi: 10.1128/AEM.02066-07

    Figure Lengend Snippet: Transcriptional profile of AI-2-treated cells versus the negative control determined by microarray analysis. Genes with negative z scores are upregulated in the AI-2-treated samples.

    Article Snippet: Each of the 4,158 probes (MetaBion GmbH, Martinsried, Germany) was printed in duplicate onto microarray slides (Sigmascreen; Sigma).

    Techniques: Negative Control, Microarray

    Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent microarray chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Rank correlation of log-ratios between intra- and inter-platform replications. For each platform, microRNA expression profiles in the liver and the prostate were measured three times by independent microarray chips. Therefore, 9 ( = 3×3) combinations of log-ratios (liver/prostate) for each microRNA was calculated. Then, 81 ( = 9×9) Spearman's correlation coefficients (Rs) values were calculated, and visualized in blue-white heat map. White indicates high correlation, whereas blue means low correlation. Heatmaps by Pearson's and Kendall's correlation coefficients were available in Figure S4 .

    Article Snippet: (5) Toray microarray Microarray slides were scanned using ProScanArray™ microarray scanner where the photomultiplier settings of the red channel were manually adjusted according to the procedures recommended by the manufacturer.

    Techniques: Expressing, Microarray

    MicroRNA expression level in human liver and prostate tissues. For the microarray platforms, log2 transformed values of representative signal intensity for detection call-positive microRNAs were plotted. For the Taqman analysis, Ct values of microRNAs were plotted. Red and blue lines indicate Y = X line and regressed linear line, respectively. In all scatter plots. blue lines were shifted upward, which indicated that the general microRNA expression level in human prostate was higher than in human liver.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: MicroRNA expression level in human liver and prostate tissues. For the microarray platforms, log2 transformed values of representative signal intensity for detection call-positive microRNAs were plotted. For the Taqman analysis, Ct values of microRNAs were plotted. Red and blue lines indicate Y = X line and regressed linear line, respectively. In all scatter plots. blue lines were shifted upward, which indicated that the general microRNA expression level in human prostate was higher than in human liver.

    Article Snippet: (5) Toray microarray Microarray slides were scanned using ProScanArray™ microarray scanner where the photomultiplier settings of the red channel were manually adjusted according to the procedures recommended by the manufacturer.

    Techniques: Expressing, Microarray, Transformation Assay

    Correlation between microarray and Taqman data. The scatter plots compare the log-ratio differential expression values from each microarray platform relative to values obtained by the Taqman assays. Each point represents a microRNA that was measured on both the microarray and Taqman assays. Only microRNAs that were generally detected in both human liver and prostate were used in the comparisons. Among 142 total microRNAs assayed by the Taqman system, the number of microRNAs analyzed for correlation to the Taqman assays are listed in the table. The red and blue lines shown are the ideal Y = X line, and the regressed line from the scatter plots, respectively. Spearman's correlation coefficients (Rs), slope and Y intercept of regressed line were shown in the table.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Correlation between microarray and Taqman data. The scatter plots compare the log-ratio differential expression values from each microarray platform relative to values obtained by the Taqman assays. Each point represents a microRNA that was measured on both the microarray and Taqman assays. Only microRNAs that were generally detected in both human liver and prostate were used in the comparisons. Among 142 total microRNAs assayed by the Taqman system, the number of microRNAs analyzed for correlation to the Taqman assays are listed in the table. The red and blue lines shown are the ideal Y = X line, and the regressed line from the scatter plots, respectively. Spearman's correlation coefficients (Rs), slope and Y intercept of regressed line were shown in the table.

    Article Snippet: (5) Toray microarray Microarray slides were scanned using ProScanArray™ microarray scanner where the photomultiplier settings of the red channel were manually adjusted according to the procedures recommended by the manufacturer.

    Techniques: Microarray, Expressing

    Agreement of microRNA list between microarray platforms and the Taqman assay. MicroRNAs that were listed, or not listed in both microarray and the Taqman assay, were considered as true positive (TP) or true negative (TN), respectively. MicroRNAs that were listed in either microarray or Taqman assays, were handled as false positive (FP) or false negative (FN), respectively. 7A and B: These graphs indicated the concordance of detection call between microarray platforms and the Taqman assay, using 142 microRNAs assayed by both microarray and the Taqman system. 7C: This bar graph demonstrated the concordance of microRNAs identified as differentially expressed between microarray platforms and the Taqman assay system.

    Journal: PLoS ONE

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

    doi: 10.1371/journal.pone.0005540

    Figure Lengend Snippet: Agreement of microRNA list between microarray platforms and the Taqman assay. MicroRNAs that were listed, or not listed in both microarray and the Taqman assay, were considered as true positive (TP) or true negative (TN), respectively. MicroRNAs that were listed in either microarray or Taqman assays, were handled as false positive (FP) or false negative (FN), respectively. 7A and B: These graphs indicated the concordance of detection call between microarray platforms and the Taqman assay, using 142 microRNAs assayed by both microarray and the Taqman system. 7C: This bar graph demonstrated the concordance of microRNAs identified as differentially expressed between microarray platforms and the Taqman assay system.

    Article Snippet: (5) Toray microarray Microarray slides were scanned using ProScanArray™ microarray scanner where the photomultiplier settings of the red channel were manually adjusted according to the procedures recommended by the manufacturer.

    Techniques: Microarray, TaqMan Assay

    Correlation of TrkC with CRC pathogenesis and patient survival (A) Box-and-whisker (Tukey) plots of the mean expression of TrkC and NT-3 in CRC patients. TrkC and NT-3 levels were extracted from the Skrzypczak microarray dataset (GSE20916) and averaged in each tumor. Points below and above the whiskers are drawn as individual dots. P

    Journal: Oncotarget

    Article Title: TrkC promotes colorectal cancer growth and metastasis

    doi: 10.18632/oncotarget.17289

    Figure Lengend Snippet: Correlation of TrkC with CRC pathogenesis and patient survival (A) Box-and-whisker (Tukey) plots of the mean expression of TrkC and NT-3 in CRC patients. TrkC and NT-3 levels were extracted from the Skrzypczak microarray dataset (GSE20916) and averaged in each tumor. Points below and above the whiskers are drawn as individual dots. P

    Article Snippet: Immunohistochemistry For human tissues, a tissue microarray slide (CO702b) was purchased from US Biomax.

    Techniques: Whisker Assay, Expressing, Microarray

    The correlation between RACK1 and Nanog expression in clinical HCC tissues. ( A ) 136 formaldehyde-fixed and paraffin-embedded clinical HCC samples were subjected to immunohistochemistry for RACK1 and Nanog staining on tissue microarray slides. Representative paired samples are shown. Scale bar: 50 μm. ( B ) Box plot of Nanog expression in HCC based on RACK1 expression in the tumors as revealed by immunohistochemistry. Kruskal-Wallis P =0.0009. ( C ) The protein levels of RACK1 positively correlated with those of Nanog in clinical HCC tissues as revealed by immunohistochemistry. Spearman r =0.2921, P =0.0006. ( D ) Fluorescent multiplex immunohistochemistry (mIHC) with tyramide signal amplification was performed on tissue microarray slides. Representative clinical HCC samples are shown. Scale bar: 400 μm.

    Journal: Theranostics

    Article Title: RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog

    doi: 10.7150/thno.29271

    Figure Lengend Snippet: The correlation between RACK1 and Nanog expression in clinical HCC tissues. ( A ) 136 formaldehyde-fixed and paraffin-embedded clinical HCC samples were subjected to immunohistochemistry for RACK1 and Nanog staining on tissue microarray slides. Representative paired samples are shown. Scale bar: 50 μm. ( B ) Box plot of Nanog expression in HCC based on RACK1 expression in the tumors as revealed by immunohistochemistry. Kruskal-Wallis P =0.0009. ( C ) The protein levels of RACK1 positively correlated with those of Nanog in clinical HCC tissues as revealed by immunohistochemistry. Spearman r =0.2921, P =0.0006. ( D ) Fluorescent multiplex immunohistochemistry (mIHC) with tyramide signal amplification was performed on tissue microarray slides. Representative clinical HCC samples are shown. Scale bar: 400 μm.

    Article Snippet: The correlation between RACK1 and Nanog expression in clinical HCC tissues The protein levels of RACK1 and Nanog in clinical HCC tissues were analyzed by immunohistochemical staining on tissue microarray slides.

    Techniques: Expressing, Immunohistochemistry, Staining, Microarray, Fluorescent Multiplex Immunohistochemistry, Amplification