microaerobic conditions Search Results


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  • 99
    Thermo Fisher campygen plus
    Campygen Plus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore brucella agar
    Brucella Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher microaerobic conditions
    Growth and survival of ( a ) C. jejuni and ( b ) C. coli strains in full strength (100%) retail juices and MHB at 37 °C in <t>microaerobic</t> condition for eight days. Statistical analyses and standard error bars are based on mean values of log CFU/ml from triplicate experiments.
    Microaerobic Conditions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Mitsubishi Gas Chemical Company microaerobic conditions
    Growth and survival of ( a ) C. jejuni and ( b ) C. coli strains in full strength (100%) retail juices and MHB at 37 °C in <t>microaerobic</t> condition for eight days. Statistical analyses and standard error bars are based on mean values of log CFU/ml from triplicate experiments.
    Microaerobic Conditions, supplied by Mitsubishi Gas Chemical Company, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Advanced Instruments Inc microaerobic conditions
    Growth and survival of ( a ) C. jejuni and ( b ) C. coli strains in full strength (100%) retail juices and MHB at 37 °C in <t>microaerobic</t> condition for eight days. Statistical analyses and standard error bars are based on mean values of log CFU/ml from triplicate experiments.
    Microaerobic Conditions, supplied by Advanced Instruments Inc, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Becton Dickinson microaerobic conditions
    Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. <t>Microaerobic:</t> 5% O 2 ; Anaerobic:
    Microaerobic Conditions, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Difco microaerobic conditions
    Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. <t>Microaerobic:</t> 5% O 2 ; Anaerobic:
    Microaerobic Conditions, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA microaerobic conditions
    Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. <t>Microaerobic:</t> 5% O 2 ; Anaerobic:
    Microaerobic Conditions, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    BOC Sciences microaerobic conditions
    Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. <t>Microaerobic:</t> 5% O 2 ; Anaerobic:
    Microaerobic Conditions, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore microaerobic conditions
    Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. <t>Microaerobic:</t> 5% O 2 ; Anaerobic:
    Microaerobic Conditions, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Air Liquide microaerobic conditions
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Microaerobic Conditions, supplied by Air Liquide, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    bioMerieux microaerobic conditions
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Microaerobic Conditions, supplied by bioMerieux, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    bioMerieux genbox anaer
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Genbox Anaer, supplied by bioMerieux, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher br38 gaspak system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Br38 Gaspak System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Merck & Co anaerocult c bags
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Anaerocult C Bags, supplied by Merck & Co, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson campy pouch system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Campy Pouch System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co anaerocult c
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Anaerocult C, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco mueller hinton mh agar
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Mueller Hinton Mh Agar, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher campypak system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Campypak System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher campygen packs
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Campygen Packs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher campygen gas packs
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Campygen Gas Packs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher campygen system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Campygen System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson gaspak jar
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Gaspak Jar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Becton Dickinson gaspak ez campypouch system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Gaspak Ez Campypouch System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher campygen 2 5l
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Campygen 2 5l, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Mitsubishi Gas Chemical Company gaspak jars
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Gaspak Jars, supplied by Mitsubishi Gas Chemical Company, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Merck & Co gas pack c
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Gas Pack C, supplied by Merck & Co, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Advanced Instruments Inc anoxomat
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Anoxomat, supplied by Advanced Instruments Inc, used in various techniques. Bioz Stars score: 92/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Becton Dickinson gazpack ez system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Gazpack Ez System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 83/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher microaerobic atmosphere generating system
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Microaerobic Atmosphere Generating System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck & Co anaerocult c pack
    Motility of C. jejuni 81–176 on BHI+0.6% agar in <t>microaerobic</t> (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.
    Anaerocult C Pack, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Growth and survival of ( a ) C. jejuni and ( b ) C. coli strains in full strength (100%) retail juices and MHB at 37 °C in microaerobic condition for eight days. Statistical analyses and standard error bars are based on mean values of log CFU/ml from triplicate experiments.

    Journal: Scientific Reports

    Article Title: Retail liver juices enhance the survivability of Campylobacter jejuni and Campylobacter coli at low temperatures

    doi: 10.1038/s41598-018-35820-7

    Figure Lengend Snippet: Growth and survival of ( a ) C. jejuni and ( b ) C. coli strains in full strength (100%) retail juices and MHB at 37 °C in microaerobic condition for eight days. Statistical analyses and standard error bars are based on mean values of log CFU/ml from triplicate experiments.

    Article Snippet: The eleven strains were subcultured from −70 °C stock cultures and grown on Mueller Hinton Agar (MHA) supplemented with 5% laked horse blood at 42 °C for 48 h in microaerobic conditions (6% O2 , 13% CO2 , 81% N2 , Thermo Forma incubator, model 3130).

    Techniques:

    Biofilm formation by ( a ) C. jejuni and ( b ) C. coli . Strains were incubated at 37 °C in MHB and retail juices (BLJ, CLJ, BJ, CJ) on polystyrene in microaerobic conditions. ( c ) Biofilm assay on borosilicate glass was conducted with C. jejuni NCTC11168. Error bars represent the standard error of mean values of absorbance (OD 492 ). Significance: * P

    Journal: Scientific Reports

    Article Title: Retail liver juices enhance the survivability of Campylobacter jejuni and Campylobacter coli at low temperatures

    doi: 10.1038/s41598-018-35820-7

    Figure Lengend Snippet: Biofilm formation by ( a ) C. jejuni and ( b ) C. coli . Strains were incubated at 37 °C in MHB and retail juices (BLJ, CLJ, BJ, CJ) on polystyrene in microaerobic conditions. ( c ) Biofilm assay on borosilicate glass was conducted with C. jejuni NCTC11168. Error bars represent the standard error of mean values of absorbance (OD 492 ). Significance: * P

    Article Snippet: The eleven strains were subcultured from −70 °C stock cultures and grown on Mueller Hinton Agar (MHA) supplemented with 5% laked horse blood at 42 °C for 48 h in microaerobic conditions (6% O2 , 13% CO2 , 81% N2 , Thermo Forma incubator, model 3130).

    Techniques: Incubation, Biofilm Production Assay

    Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. Microaerobic: 5% O 2 ; Anaerobic:

    Journal: Scientific Reports

    Article Title: Formate simultaneously reduces oxidase activity and enhances respiration in Campylobacter jejuni

    doi: 10.1038/srep40117

    Figure Lengend Snippet: Biofilm formation of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, and fumarate, respectively. Biofilm formation assays were performed using borosilicate tubes and crystal violet staining. After staining, the biofilms were suspended in DMSO (80%) and quantified using a spectrophotometer. Microaerobic: 5% O 2 ; Anaerobic:

    Article Snippet: C. jejuni strains and culture conditions C. jejuni 81–176 (wildtype) was cultured on Mueller-Hinton (MH) agar at 42 °C under microaerobic conditions (85% N2 , 10% CO2 , 5% O2 ) or under < 1% O2 (hereafter referred to as anaerobic conditions) which was generated using the BD GasPak Sachets system (BD Diagnostics, NJ, USA) .

    Techniques: Staining, Spectrophotometry

    ( a) Respiratory activity of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, or fumarate under microaerobic conditions.. The respiratory activity was determined using CTC (5-cyano-2,3-ditolyltetrazolium chloride), which is reduced to formazan. This colorimetric reaction was then quantified using a spectrophotometer. ( b) Respiratory activity of C. jejuni 81–176 in Mueller-Hinton broth supplemented with formate and nitrate. Anaero:

    Journal: Scientific Reports

    Article Title: Formate simultaneously reduces oxidase activity and enhances respiration in Campylobacter jejuni

    doi: 10.1038/srep40117

    Figure Lengend Snippet: ( a) Respiratory activity of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate, succinate, or fumarate under microaerobic conditions.. The respiratory activity was determined using CTC (5-cyano-2,3-ditolyltetrazolium chloride), which is reduced to formazan. This colorimetric reaction was then quantified using a spectrophotometer. ( b) Respiratory activity of C. jejuni 81–176 in Mueller-Hinton broth supplemented with formate and nitrate. Anaero:

    Article Snippet: C. jejuni strains and culture conditions C. jejuni 81–176 (wildtype) was cultured on Mueller-Hinton (MH) agar at 42 °C under microaerobic conditions (85% N2 , 10% CO2 , 5% O2 ) or under < 1% O2 (hereafter referred to as anaerobic conditions) which was generated using the BD GasPak Sachets system (BD Diagnostics, NJ, USA) .

    Techniques: Activity Assay, Spectrophotometry

    Aerotolerance of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate. The cultures were shifted from microaerobic conditions to ambient oxygen conditions with shaking (300 rpm). OD 600 was measured at different time points and CFU numbers were quantified at 8 h of incubation under ambient oxygen. Asterisk * indicates statistically significant differences ( P

    Journal: Scientific Reports

    Article Title: Formate simultaneously reduces oxidase activity and enhances respiration in Campylobacter jejuni

    doi: 10.1038/srep40117

    Figure Lengend Snippet: Aerotolerance of C. jejuni 81–176 in Mueller-Hinton broth supplemented with different concentrations of formate. The cultures were shifted from microaerobic conditions to ambient oxygen conditions with shaking (300 rpm). OD 600 was measured at different time points and CFU numbers were quantified at 8 h of incubation under ambient oxygen. Asterisk * indicates statistically significant differences ( P

    Article Snippet: C. jejuni strains and culture conditions C. jejuni 81–176 (wildtype) was cultured on Mueller-Hinton (MH) agar at 42 °C under microaerobic conditions (85% N2 , 10% CO2 , 5% O2 ) or under < 1% O2 (hereafter referred to as anaerobic conditions) which was generated using the BD GasPak Sachets system (BD Diagnostics, NJ, USA) .

    Techniques: Incubation

    Motility of C. jejuni 81–176 on BHI+0.6% agar in microaerobic (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.

    Journal: PLoS ONE

    Article Title: Enhanced Adhesion of Campylobacter jejuni to Abiotic Surfaces Is Mediated by Membrane Proteins in Oxygen-Enriched Conditions

    doi: 10.1371/journal.pone.0046402

    Figure Lengend Snippet: Motility of C. jejuni 81–176 on BHI+0.6% agar in microaerobic (5% O 2 ) and oxygen-enriched conditions (19% O 2 ) after 48 h at 42°C. Assays were performed with 2 µL of pure culture or 10 times diluted culture. (A) Mean diameters of three independent experiments. (B) Example of motility plates obtained after 48 h at 42°C with a diluted culture.

    Article Snippet: A loopful of frozen culture conserved at −80°C in Brain-Heart Infusion (BHI) broth (Biokar, Beauvais, France) containing 20% sterile glycerol was spread on fresh Karmali agar plates (Oxoid, Dardilly, France) and incubated in microaerobic conditions of 5% O2 , 10% CO2 and 85% N2 (Air Liquide, Paris, France) at 42°C for 48 h. Then, a subculture was performed in BHI in 24-well plates incubated for 18 h at 42°C under microaerobic conditions (5% O2 , 10% CO2 and 85% N2 , Air Liquide) or oxygen-acclimated conditions (19% O2 , 10% CO2 , and 71% N2 , Air Liquide) with shaking.

    Techniques: