mi 49930 Search Results


box 65  (ATCC)
93
ATCC box 65
Box 65, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/box 65/product/ATCC
Average 93 stars, based on 1 article reviews
box 65 - by Bioz Stars, 2025-04
93/100 stars
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lama5  (Thermo Fisher)
86
Thermo Fisher lama5
(A and B) Primary human LECs exposed to OSS for 24 hours became cuboidal shapes. (A) Immunofluorescence staining for F-actin and (B) a box-and-whisker plot showing the ratio of length/width of the cells. (C and D) OSS upregulated FOXC2, GATA2, CX37, <t>LAMA5,</t> and ITGA9 in LECs, based on (C) Western blot analyses and (D) quantitative PCR (qPCR). The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. (E and F) Piezo1 knockdown prevented the OSS-mediated upregulation of lymphatic valve genes. Piezo1 expression in primary LECs was inhibited by Piezo1 siRNA (siPiezo1), or not inhibited by control siRNA (siCTR), for 24 hours. Cells were then exposed to OSS for (E) 4 hours or (F) 24 hours before (E) qPCR data assay or (F) Western blot analysis, respectively. OSS with approximately at 6 dyne/cm2 maximum was applied directly onto the cells by reversing the flow at 0.5 Hz (peak). **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test compared with the static culture.
Lama5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lama5/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
lama5 - by Bioz Stars, 2025-04
86/100 stars
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3 4 dihydro 2 2 dimethyl 4 oxo 2h 1 benzopyran 6 carboxylic acid  (Santa Cruz Biotechnology)
N/A
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1-(5-fluoro-1h-indol-3-yl)propan-2-one  (Biosynth Carbosynth)
N/A
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5β dihydrocortisol  (Santa Cruz Biotechnology)
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Image Search Results


(A and B) Primary human LECs exposed to OSS for 24 hours became cuboidal shapes. (A) Immunofluorescence staining for F-actin and (B) a box-and-whisker plot showing the ratio of length/width of the cells. (C and D) OSS upregulated FOXC2, GATA2, CX37, LAMA5, and ITGA9 in LECs, based on (C) Western blot analyses and (D) quantitative PCR (qPCR). The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. (E and F) Piezo1 knockdown prevented the OSS-mediated upregulation of lymphatic valve genes. Piezo1 expression in primary LECs was inhibited by Piezo1 siRNA (siPiezo1), or not inhibited by control siRNA (siCTR), for 24 hours. Cells were then exposed to OSS for (E) 4 hours or (F) 24 hours before (E) qPCR data assay or (F) Western blot analysis, respectively. OSS with approximately at 6 dyne/cm2 maximum was applied directly onto the cells by reversing the flow at 0.5 Hz (peak). **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test compared with the static culture.

Journal: JCI Insight

Article Title: Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance

doi: 10.1172/jci.insight.125068

Figure Lengend Snippet: (A and B) Primary human LECs exposed to OSS for 24 hours became cuboidal shapes. (A) Immunofluorescence staining for F-actin and (B) a box-and-whisker plot showing the ratio of length/width of the cells. (C and D) OSS upregulated FOXC2, GATA2, CX37, LAMA5, and ITGA9 in LECs, based on (C) Western blot analyses and (D) quantitative PCR (qPCR). The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. (E and F) Piezo1 knockdown prevented the OSS-mediated upregulation of lymphatic valve genes. Piezo1 expression in primary LECs was inhibited by Piezo1 siRNA (siPiezo1), or not inhibited by control siRNA (siCTR), for 24 hours. Cells were then exposed to OSS for (E) 4 hours or (F) 24 hours before (E) qPCR data assay or (F) Western blot analysis, respectively. OSS with approximately at 6 dyne/cm2 maximum was applied directly onto the cells by reversing the flow at 0.5 Hz (peak). **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test compared with the static culture.

Article Snippet: The sources of the reagents are: Yoda1 (R&D Systems); tamoxifen (MP Biomedicals); and antibodies for FOXC2 (R&D Systems, AF5044), GATA2 (R&D Systems, AF2046), LAMA5 (Thermo Fisher Scientific, PA5-49930), CX37 (Abcam, ab181701), ITGA9 (R&D Systems, MAB4574), RFP/tdTomato (Rockland, 600-401-379), and β-actin (MilliporeSigma, A5441).

Techniques: Immunofluorescence, Staining, Whisker Assay, Western Blot, Real-time Polymerase Chain Reaction, Expressing

(A and B) Primary LECs were transfected with a Piezo1/EGFP-expressing plasmid and cultured for 48 hours without OSS, before immunostaining for lymphatic valve genes FOXC2, GATA2, CX37, LAMA5, and ITGA9. (A) Arrowheads point to transfected EGFP+ (thus Piezo1-overexpressing) cells, whereas arrows point to EGFP–, untransfected cells. O/E, overexpression; LV, lymphatic valve. (B) The intensity of these cells was measured and charted in a box-and-whisker plot. (C and D) Expression of the lymphatic valve genes in LECs that were transfected with a control (CTR) or Piezo1/EGFP-expressing plasmid (Piezo1) was determined by qRT-PCR after (C) 24 hours, or by Western blot assay after (D) 48 hours, in the absence of OSS. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. Scale bars: 50 μm (A). **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test compared with (B) the untransfected cells or (C) the control plasmid.

Journal: JCI Insight

Article Title: Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance

doi: 10.1172/jci.insight.125068

Figure Lengend Snippet: (A and B) Primary LECs were transfected with a Piezo1/EGFP-expressing plasmid and cultured for 48 hours without OSS, before immunostaining for lymphatic valve genes FOXC2, GATA2, CX37, LAMA5, and ITGA9. (A) Arrowheads point to transfected EGFP+ (thus Piezo1-overexpressing) cells, whereas arrows point to EGFP–, untransfected cells. O/E, overexpression; LV, lymphatic valve. (B) The intensity of these cells was measured and charted in a box-and-whisker plot. (C and D) Expression of the lymphatic valve genes in LECs that were transfected with a control (CTR) or Piezo1/EGFP-expressing plasmid (Piezo1) was determined by qRT-PCR after (C) 24 hours, or by Western blot assay after (D) 48 hours, in the absence of OSS. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. Scale bars: 50 μm (A). **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test compared with (B) the untransfected cells or (C) the control plasmid.

Article Snippet: The sources of the reagents are: Yoda1 (R&D Systems); tamoxifen (MP Biomedicals); and antibodies for FOXC2 (R&D Systems, AF5044), GATA2 (R&D Systems, AF2046), LAMA5 (Thermo Fisher Scientific, PA5-49930), CX37 (Abcam, ab181701), ITGA9 (R&D Systems, MAB4574), RFP/tdTomato (Rockland, 600-401-379), and β-actin (MilliporeSigma, A5441).

Techniques: Transfection, Expressing, Plasmid Preparation, Cell Culture, Immunostaining, Over Expression, Whisker Assay, Quantitative RT-PCR, Western Blot

(A and B) Regulation of (A) mRNA and (B) protein of lymphatic valve genes in LECs by Yoda1 was determined by qPCR and Western blot analyses, respectively. Primary human LECs were treated by Yoda1 at the indicated concentrations for 24 hours. Yoda1 upregulated the expression of GATA2, CX37, LAMA5, and ITGA9 but not FOXC2 and PROX1. (C and D) Piezo1 is required for the Yoda1-induced upregulation of GATA2, CX37, and LAMA5 but not ITGA9. LECs were transfected with control siRNA (siCTR) or Piezo1 siRNA (siPiezo1) for 24 hours, followed by Yoda1 treatment (250 nM) for 24 hours, before isolation of (C) RNA or (D) whole-cell lysates for qPCR and Western blot analyses, respectively. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. *P < 0.05; **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test.

Journal: JCI Insight

Article Title: Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance

doi: 10.1172/jci.insight.125068

Figure Lengend Snippet: (A and B) Regulation of (A) mRNA and (B) protein of lymphatic valve genes in LECs by Yoda1 was determined by qPCR and Western blot analyses, respectively. Primary human LECs were treated by Yoda1 at the indicated concentrations for 24 hours. Yoda1 upregulated the expression of GATA2, CX37, LAMA5, and ITGA9 but not FOXC2 and PROX1. (C and D) Piezo1 is required for the Yoda1-induced upregulation of GATA2, CX37, and LAMA5 but not ITGA9. LECs were transfected with control siRNA (siCTR) or Piezo1 siRNA (siPiezo1) for 24 hours, followed by Yoda1 treatment (250 nM) for 24 hours, before isolation of (C) RNA or (D) whole-cell lysates for qPCR and Western blot analyses, respectively. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. *P < 0.05; **P < 0.01; ***P < 0.001; unpaired, 2-tailed t test.

Article Snippet: The sources of the reagents are: Yoda1 (R&D Systems); tamoxifen (MP Biomedicals); and antibodies for FOXC2 (R&D Systems, AF5044), GATA2 (R&D Systems, AF2046), LAMA5 (Thermo Fisher Scientific, PA5-49930), CX37 (Abcam, ab181701), ITGA9 (R&D Systems, MAB4574), RFP/tdTomato (Rockland, 600-401-379), and β-actin (MilliporeSigma, A5441).

Techniques: Western Blot, Expressing, Transfection, Isolation