Journal: PLoS ONE
Article Title: Insights into the Molecular Basis of L-Form Formation and Survival in Escherichia coli
Figure Lengend Snippet: Comparison of L-form E. coli and classical E. coli morphologies. A E. coli colony on L-form induction media (LIM) exhibiting typical “fried egg” morphology. B Classical E. coli colony on Brain Heart Infusion (BHI) agar. C E. coli colony on BHI+ 10% Sucrose and 0.125% MgSO4 (BHI+ sucrose control media). D E. coli penicillin G mutant colony on BHI+ Pen G. E Phase contrast of rod-shaped E. coli cells within a classical colony. Scale bar, 3 µm. F Phase contrast of coccoid cells within E. coli L-form colony agar squash. Scale bar, 3 µm. G Individual coccoid cells in soft agar LIM. Scale bar, 10 µm. H Transmission electron microscopy (TEM) photo of a coccoid cell within an L-form colony. Scale bar, 200 nm. All images are by Hoffman modulation and scale bars are 100 µm unless specified.
Article Snippet: Undiluted along with serial tenfold dilutions of cells were spread (100 µl) or spotted (10 µl) onto L-form induction media (LIM) which consisted of brain heart infusion broth (BHI) Becton Dickinson (BD) supplemented with 1% agar (BD), 10% sucrose, 0.125% MgSO4 , and 6000 µg (10,000 units )/ml of Penicillin G (Sigma).
Techniques: Mutagenesis, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy