mgso4 Integrated Dna Technologies Search Results


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  • 99
    New England Biolabs magnesium sulfate mgso4 solution
    Magnesium Sulfate Mgso4 Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mgso4
    Mgso4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 7247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio Basic Canada mgso4
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    New England Biolabs mgso4
    Mgso4, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega mgso4
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    Eurofins idt
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    Integrated DNA Technologies idt scitools
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    Millipore total mgso4
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    Thermo Fisher mgso4 7h2 o
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    Fisher Bioreagents mgso4
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    Merck & Co mgso4
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    PRIMER-E mgso4
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    Integrated DNA Technologies sal4 lb
    Sal4 Lb, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies b3
    B3, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies oligodeoxynucleotide primer
    Oligodeoxynucleotide Primer, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies digoxigenin labeled oligonucleotides
    Determining RIPs at the DBF4 locus in asynchronous HeLa cells. ( a ) Primer extension with the indicated <t>digoxigenin-labeled</t> primers (pA, pB and pC) using nascent DNA (1–2 kb) isolated from asynchronous HeLa cells that had been incubated for 1 h
    Digoxigenin Labeled Oligonucleotides, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Integrated DNA Technologies primer
    Determining RIPs at the DBF4 locus in asynchronous HeLa cells. ( a ) Primer extension with the indicated <t>digoxigenin-labeled</t> primers (pA, pB and pC) using nascent DNA (1–2 kb) isolated from asynchronous HeLa cells that had been incubated for 1 h
    Primer, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 2961 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies synthetic double stranded dna
    Determining RIPs at the DBF4 locus in asynchronous HeLa cells. ( a ) Primer extension with the indicated <t>digoxigenin-labeled</t> primers (pA, pB and pC) using nascent DNA (1–2 kb) isolated from asynchronous HeLa cells that had been incubated for 1 h
    Synthetic Double Stranded Dna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 88/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies antisense primer
    Determining RIPs at the DBF4 locus in asynchronous HeLa cells. ( a ) Primer extension with the indicated <t>digoxigenin-labeled</t> primers (pA, pB and pC) using nascent DNA (1–2 kb) isolated from asynchronous HeLa cells that had been incubated for 1 h
    Antisense Primer, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies template dna
    Representative active site conformation of (a) <t>E295K</t> mutant and (b) wild-type pol β in the final closed form. Dashed lines indicate hydrogen bonds. Mg(A), catalytic ion; Mg(B), nucleotide-binding ion; C10, the base in the upstream primer <t>DNA</t> strand
    Template Dna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies per1
    <t>Per1</t> gene activity is necessary for GRP-induced increases in neuronal activity of Per1 -expressing neurons. A , Examples of individual, extracellular recordings from slices that were treated with either vehicle (top trace) or GRP (bottom trace) at projected ZT 21. Calibration: 40 pA, 1.0 s. B , Bar graph indicating the mean spontaneous action potential (A.P.) frequency of Per1 fluorescent neurons after treatment with either vehicle or GRP at projected ZT 21. * p
    Per1, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies homepage
    <t>Per1</t> gene activity is necessary for GRP-induced increases in neuronal activity of Per1 -expressing neurons. A , Examples of individual, extracellular recordings from slices that were treated with either vehicle (top trace) or GRP (bottom trace) at projected ZT 21. Calibration: 40 pA, 1.0 s. B , Bar graph indicating the mean spontaneous action potential (A.P.) frequency of Per1 fluorescent neurons after treatment with either vehicle or GRP at projected ZT 21. * p
    Homepage, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies primerquestsm
    <t>Per1</t> gene activity is necessary for GRP-induced increases in neuronal activity of Per1 -expressing neurons. A , Examples of individual, extracellular recordings from slices that were treated with either vehicle (top trace) or GRP (bottom trace) at projected ZT 21. Calibration: 40 pA, 1.0 s. B , Bar graph indicating the mean spontaneous action potential (A.P.) frequency of Per1 fluorescent neurons after treatment with either vehicle or GRP at projected ZT 21. * p
    Primerquestsm, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 89/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher probes
    <t>Per1</t> gene activity is necessary for GRP-induced increases in neuronal activity of Per1 -expressing neurons. A , Examples of individual, extracellular recordings from slices that were treated with either vehicle (top trace) or GRP (bottom trace) at projected ZT 21. Calibration: 40 pA, 1.0 s. B , Bar graph indicating the mean spontaneous action potential (A.P.) frequency of Per1 fluorescent neurons after treatment with either vehicle or GRP at projected ZT 21. * p
    Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Determining RIPs at the DBF4 locus in asynchronous HeLa cells. ( a ) Primer extension with the indicated digoxigenin-labeled primers (pA, pB and pC) using nascent DNA (1–2 kb) isolated from asynchronous HeLa cells that had been incubated for 1 h

    Journal:

    Article Title: Asymmetric bidirectional replication at the human DBF4 origin

    doi: 10.1038/nsmb.1439

    Figure Lengend Snippet: Determining RIPs at the DBF4 locus in asynchronous HeLa cells. ( a ) Primer extension with the indicated digoxigenin-labeled primers (pA, pB and pC) using nascent DNA (1–2 kb) isolated from asynchronous HeLa cells that had been incubated for 1 h

    Article Snippet: One-way PCR reactions were carried out in a final volume of 30 μl containing 1×PCR buffer (ID Labs Biotechnology), 200 μM dNTPs, 3 mM MgSO4 , 400 nM digoxigenin-labeled oligonucleotides (Integrated DNA Technologies), 1 M betaine (Sigma) and 1 unit of IDPol DNA polymerase (ID Labs Biotechnology).

    Techniques: Labeling, Isolation, Incubation

    Representative active site conformation of (a) E295K mutant and (b) wild-type pol β in the final closed form. Dashed lines indicate hydrogen bonds. Mg(A), catalytic ion; Mg(B), nucleotide-binding ion; C10, the base in the upstream primer DNA strand

    Journal: Journal of the American Chemical Society

    Article Title: Unfavorable electrostatic and steric interactions in DNA polymerase ? E295K mutant interfere with the enzyme's pathway

    doi: 10.1021/ja300361r

    Figure Lengend Snippet: Representative active site conformation of (a) E295K mutant and (b) wild-type pol β in the final closed form. Dashed lines indicate hydrogen bonds. Mg(A), catalytic ion; Mg(B), nucleotide-binding ion; C10, the base in the upstream primer DNA strand

    Article Snippet: For E295K co-crystallization trials, the primer (5′-ATG TGA G-3′) and template DNA (5′-CAA ACT CAC AT-3′) (Integrated DNA Technology) were resuspended in water and annealed 1:1 in 20 mM MgSO4 (90°C for 2 minutes, 70°C for 2 minutes, 55°C for 1 minute, then cooled to 4°C by decreasing 0.5°C every cycle) in a DNA Dyad Peltier Thermal Cycler (MJ Research, Inc).

    Techniques: Mutagenesis, Binding Assay

    Per1 gene activity is necessary for GRP-induced increases in neuronal activity of Per1 -expressing neurons. A , Examples of individual, extracellular recordings from slices that were treated with either vehicle (top trace) or GRP (bottom trace) at projected ZT 21. Calibration: 40 pA, 1.0 s. B , Bar graph indicating the mean spontaneous action potential (A.P.) frequency of Per1 fluorescent neurons after treatment with either vehicle or GRP at projected ZT 21. * p

    Journal: The Journal of Neuroscience

    Article Title: Gastrin-Releasing Peptide Mediates Light-Like Resetting of the Suprachiasmatic Nucleus Circadian Pacemaker through cAMP Response Element-Binding Protein and Per1 Activation

    doi: 10.1523/JNEUROSCI.1109-07.2007

    Figure Lengend Snippet: Per1 gene activity is necessary for GRP-induced increases in neuronal activity of Per1 -expressing neurons. A , Examples of individual, extracellular recordings from slices that were treated with either vehicle (top trace) or GRP (bottom trace) at projected ZT 21. Calibration: 40 pA, 1.0 s. B , Bar graph indicating the mean spontaneous action potential (A.P.) frequency of Per1 fluorescent neurons after treatment with either vehicle or GRP at projected ZT 21. * p

    Article Snippet: Antisense ODN inhibition of Per1 as supplemental material).

    Techniques: Activity Assay, Expressing

    Representative confocal images of Per1 ::GFP mice injected with vehicle or GRP (top) and GRP plus TTX or TTX (bottom) into the SCN region at ZT 21. Mice were perfused at ZT 24. Alternating sections (40 μm thick) were double labeled for VIP–GFP (left column of each treatment group) or AVP–GFP (right column of each treatment group). Top row, Single-labeled images for AVP immunoreactivity or VIP immunoreactivity (red); middle row, single-labeled images for GFP immunoreactivity (green); bottom row, double-labeled composite of AVP–GFP or VIP–GFP (yellow cells are double labeled).

    Journal: The Journal of Neuroscience

    Article Title: Gastrin-Releasing Peptide Mediates Light-Like Resetting of the Suprachiasmatic Nucleus Circadian Pacemaker through cAMP Response Element-Binding Protein and Per1 Activation

    doi: 10.1523/JNEUROSCI.1109-07.2007

    Figure Lengend Snippet: Representative confocal images of Per1 ::GFP mice injected with vehicle or GRP (top) and GRP plus TTX or TTX (bottom) into the SCN region at ZT 21. Mice were perfused at ZT 24. Alternating sections (40 μm thick) were double labeled for VIP–GFP (left column of each treatment group) or AVP–GFP (right column of each treatment group). Top row, Single-labeled images for AVP immunoreactivity or VIP immunoreactivity (red); middle row, single-labeled images for GFP immunoreactivity (green); bottom row, double-labeled composite of AVP–GFP or VIP–GFP (yellow cells are double labeled).

    Article Snippet: Antisense ODN inhibition of Per1 as supplemental material).

    Techniques: Mouse Assay, Injection, Labeling

    Model of GRP mediation of photic phase resetting in the SCN. During photic phase resetting, we propose that GRP cells and GRP are involved in the following ways. “Transduction of the photic signal in the SCN,” Ventral SCN cells in the night phase expressing GRP and/or VIP (top cell) show a persistent increase in the frequency of spontaneous action potentials (far right, top), as well as the induction of Per1 ). “Communication of the intra-SCN photic signal,” GRP is released from ventral cells and activates BB 2 ; this study), resulting in activation of Per1 that is necessary for a persistent increase in the frequency of spontaneous action potentials to be induced (this study). “Phase shift of SCN rhythms and behavior,” Phase advance of the electrical activity rhythm, behavior, and gene expression.

    Journal: The Journal of Neuroscience

    Article Title: Gastrin-Releasing Peptide Mediates Light-Like Resetting of the Suprachiasmatic Nucleus Circadian Pacemaker through cAMP Response Element-Binding Protein and Per1 Activation

    doi: 10.1523/JNEUROSCI.1109-07.2007

    Figure Lengend Snippet: Model of GRP mediation of photic phase resetting in the SCN. During photic phase resetting, we propose that GRP cells and GRP are involved in the following ways. “Transduction of the photic signal in the SCN,” Ventral SCN cells in the night phase expressing GRP and/or VIP (top cell) show a persistent increase in the frequency of spontaneous action potentials (far right, top), as well as the induction of Per1 ). “Communication of the intra-SCN photic signal,” GRP is released from ventral cells and activates BB 2 ; this study), resulting in activation of Per1 that is necessary for a persistent increase in the frequency of spontaneous action potentials to be induced (this study). “Phase shift of SCN rhythms and behavior,” Phase advance of the electrical activity rhythm, behavior, and gene expression.

    Article Snippet: Antisense ODN inhibition of Per1 as supplemental material).

    Techniques: Expressing, Activation Assay, Activity Assay