mgcl2 Roche Search Results


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  • 95
    Millipore mgcl2
    Mgcl2, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 39277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher mgcl2
    Mgcl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 77901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche magnesium chloride
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Magnesium Chloride, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 588 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche magnesium chloride solution
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Magnesium Chloride Solution, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Roche phosphotungstic acid magnesium chloride
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Phosphotungstic Acid Magnesium Chloride, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche hepes koh magnesium chloride
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Hepes Koh Magnesium Chloride, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    PerkinElmer mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 10314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche mgcl2 10x
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 10x, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche mmol mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mmol Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche m mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    M Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 3241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche 2 0m mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    2 0m Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche mgcl2 stock solution
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Stock Solution, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche hucv oc43 n mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Hucv Oc43 N Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Roche mgcl2 5
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 5, supplied by Roche, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche mgcl2 buffer
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche mgcl2 pcr buffer
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche mgcl2 free buffer fast start roche
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Free Buffer Fast Start Roche, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche pcr mgcl2 free buffer
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Pcr Mgcl2 Free Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Roche mgcl2 1 tablet
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 1 Tablet, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche mgcl2 plus protease
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Plus Protease, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Roche mgcl2 amplitaq gold dna polymerase
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Amplitaq Gold Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Roche mgcl2 plus protease inhibitor cocktail
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Plus Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche nacl 50mm mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Nacl 50mm Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche e13 primer pair mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    E13 Primer Pair Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche faststart taq polymerase pcr buffer mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Faststart Taq Polymerase Pcr Buffer Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche hucv 229e s1 gene mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Hucv 229e S1 Gene Mgcl2, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche mgcl2 complete mini protease inhibitor cocktail edta free
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2 Complete Mini Protease Inhibitor Cocktail Edta Free, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche buffer 10 mm tris cl at ph 7 5 4 mm mgcl2 2 mm cacl2 and complete protease inhibitor cocktail tablet
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Buffer 10 Mm Tris Cl At Ph 7 5 4 Mm Mgcl2 2 Mm Cacl2 And Complete Protease Inhibitor Cocktail Tablet, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche hypotonic low salt ls buffer buffer 10 mm hepes ph 7 5 20 mm kcl 10 mm mgcl2 complete protease inhibitor edta free tablets
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Hypotonic Low Salt Ls Buffer Buffer 10 Mm Hepes Ph 7 5 20 Mm Kcl 10 Mm Mgcl2 Complete Protease Inhibitor Edta Free Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche pcr buffer 1 roche
    IL-9 <t>RT-PCR.</t> A. Time course. PBMC were incubated for various times with OKT3, RNA was extracted, IL-9 real time RT-PCR was performed, and the mean threshold cycle (Ct) was determined. The data shown are from an experiment on one representative individual. The values are means of duplicate determinations. B. Gel electrophoresis. PBMC were incubated for 24 h with or without OKT3 and with or without Dex (10-6 M). RNA was extracted and IL-9 RT-PCR performed for 40 cycles. For each condition, duplicate PCRs were performed on <t>cDNA</t> from one representative individual. Products were analysed in a 2% agarose gel. The left lane contains HaeIII cut ΦX174 molecular size markers (Roche); the arrow indicates the position of the 281/271 bp markers.
    Pcr Buffer 1 Roche, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PCR optimization on mouse testis cDNA by MgCl2 gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).

    Journal: Journal of Reproduction & Infertility

    Article Title: Producing Recombinant mTEX101; a Murine Testis Specific Protein

    doi:

    Figure Lengend Snippet: PCR optimization on mouse testis cDNA by MgCl2 gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).

    Article Snippet: PCR amplifications for mTEX101 transcript was performed in a volume of 25µl using 10-20ng of testis cDNA, forward and reverse primers (10pmol each), specific for mTEX101 transcript, 10X PCR buffer (2.5µl ), dNTP mixture (0.2mM each), 1mM MgCl2, and 1 unit of Taq DNA polymerase (Roche, Mannheim, Germany).

    Techniques: Polymerase Chain Reaction, Negative Control

    IL-9 RT-PCR. A. Time course. PBMC were incubated for various times with OKT3, RNA was extracted, IL-9 real time RT-PCR was performed, and the mean threshold cycle (Ct) was determined. The data shown are from an experiment on one representative individual. The values are means of duplicate determinations. B. Gel electrophoresis. PBMC were incubated for 24 h with or without OKT3 and with or without Dex (10-6 M). RNA was extracted and IL-9 RT-PCR performed for 40 cycles. For each condition, duplicate PCRs were performed on cDNA from one representative individual. Products were analysed in a 2% agarose gel. The left lane contains HaeIII cut ΦX174 molecular size markers (Roche); the arrow indicates the position of the 281/271 bp markers.

    Journal: Journal of Inflammation (London, England)

    Article Title: Dexamethasone inhibits IL-9 production by human T cells

    doi: 10.1186/1476-9255-2-3

    Figure Lengend Snippet: IL-9 RT-PCR. A. Time course. PBMC were incubated for various times with OKT3, RNA was extracted, IL-9 real time RT-PCR was performed, and the mean threshold cycle (Ct) was determined. The data shown are from an experiment on one representative individual. The values are means of duplicate determinations. B. Gel electrophoresis. PBMC were incubated for 24 h with or without OKT3 and with or without Dex (10-6 M). RNA was extracted and IL-9 RT-PCR performed for 40 cycles. For each condition, duplicate PCRs were performed on cDNA from one representative individual. Products were analysed in a 2% agarose gel. The left lane contains HaeIII cut ΦX174 molecular size markers (Roche); the arrow indicates the position of the 281/271 bp markers.

    Article Snippet: 1 μL cDNA was amplified in 25 μL in PCR buffer (10 mM Tris-HCl, 1.5 mM MgCl2 , 50 mM KCl) (Roche), 0.25 mM dNTP (Roche), 1 X SybGr (Molecular Probes, Eugene, OR, USA), 0.75 U Taq polymerase (Roche), 2 mM MgCl2 and 0.32 μM of forward and reverse β-actin primer (Geneworks) with sequences 5'CCAACTGGGACGACATG3' and 5'CAGGGATAGCACAGCCT3' respectively [ ].

    Techniques: Reverse Transcription Polymerase Chain Reaction, Incubation, Quantitative RT-PCR, Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis