Journal: Frontiers in Microbiology

Article Title: Geomicrobiological Features of Ferruginous Sediments from Lake Towuti, Indonesia

doi: 10.3389/fmicb.2016.01007

Figure Lengend Snippet: Multiple profiles established on short sediment cores. For consistency, results for the three different sites are displayed vertically according to their water depths. (A) From left to right: Total organic carbon and total carbon (wt%); molar C org /N ratio measured in bulk sediments; calcium, magnesium, chloride, and sulfate concentrations (μM) measured in the pore water; potential sulfate reduction rates (nmol × cm -3 day -1 ) obtained after 24 h incubation experiments; ammonium concentrations (μM) in the pore water measured by cell dialysis. (B) From left to right: Cell counts in log scale (log 10 cells × cm -3 ); concentrations of extracellular DNA (gray dots) and total DNA (black squares) (μg × g wet sediment -1 ), with distance between the two curves corresponding to intracellular DNA concentrations; Shannon index established from bacterial and archaeal DGGE gel features, with eDNA (gray dots) and iDNA (black squares) displayed separately; range-weighted richness index for bacterial DNA (square) and archaeal DNA (dots), with eDNA (gray) and iDNA (black) displayed separately.

Article Snippet: PCR was performed with 2.5 μL of DNA template, 12.5 μL of MangoMix (Bioline, Life Science Company, London, UK), 0.5 μmol L-1 of each of the primers, 1 μL of MgCl2 (25 mM) and 8 μL of Ultra-pure 18.2 Mω PCR Water (Bioline).

Techniques: Incubation, Denaturing Gradient Gel Electrophoresis

Journal: Biochemistry

Article Title: Isozyme Specific Allosteric Regulation of Human Sulfotransferase 1A1

doi: 10.1021/acs.biochem.6b00401

Figure Lengend Snippet: EGCG binding to the E·(PAP)2 Complex. A.) The Binding-Reaction Progress Curve . Binding-induced fluorescence changes were monitored using a stopped-flow fluorimeter (λ ex = 290 nm, λ em ≥ 325 nm (cutoff filter). Reactions were initiated by rapidly mixing (1:1 v/v) a solution containing SULT1A1 (0.25μM, active sites), PAP (0.50 mM), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C with a solution that was identical except that it lacked SULT1A1 and contained EGCG. The curve through the data (red line) is the behavior predicted by the best fit to a double-exponential model. The insert shows the residuals from a single- (black) and doubleexponential (red) fit. The single-exponential model deviates substantially from the data, while the bi-phasic model provides an excellent fit. B.) k obs vs [EGCG] for the First (Fast) Phase . kobs values were obtained at the four indicated EGCG concentrations under the conditions given in Panel A. Each value is the average of three independent determinations. The reactions were pseudo-first-order in EGCG in all cases. C.) EGCG Dissociation from E·(PAP) 2 ·(EGCG) 2 Complex . A pulse solution containing SULT1A1 (20 μM, monomer), EGCG (24 μM (4.0 μM free, 100 × Kd)), and PAP (500 μM, 16 ▪ K d low affinity site ), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C, was diluted 200:1 into a chase solution containing 75 μM 4-hydroxytamoxifen (TAM, 100 ▪ K d cap open form ), PAP (0.50 mM, 16 ▪ K d low affinity site ), NaPO4 (50 mM), MgCl2 (5.0 mM), pH 7.5, 25 ± 2°C. The final EGCG concentration is 0.12 μM (0.20 ▪ K d cap open form ). Dissociation was monitored by a change in SULT1A1 fluorescence (λ ex = 290 nm, λ em = 370 nm). Three progress curves (black dots) are superposed. The curve through the data is the behavior predicted by a best-fit, single-exponential model.

Article Snippet: Ampicillin, HEPES, KCl, KOH, MgCl2 , NaCl and phenylmethylsulfonyl fluoride (PMSF) were purchased from Fisher Scientific.

Techniques: Binding Assay, Fluorescence, Flow Cytometry, Concentration Assay