Journal: Journal of Reproduction & Infertility

Article Title: Producing Recombinant mTEX101; a Murine Testis Specific Protein

doi:

Figure Lengend Snippet: PCR optimization on mouse testis cDNA by MgCl2 gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).

Article Snippet: PCR amplifications for mTEX101 transcript was performed in a volume of 25µl using 10-20ng of testis cDNA, forward and reverse primers (10pmol each), specific for mTEX101 transcript, 10X PCR buffer (2.5µl ), dNTP mixture (0.2mM each), 1mM MgCl2, and 1 unit of Taq DNA polymerase (Roche, Mannheim, Germany).

Techniques: Polymerase Chain Reaction, Negative Control

Journal: Biological Procedures Online

Article Title: Rapid and simple comparison of messenger RNA levels using real-time PCR

doi: 10.1251/bpo114

Figure Lengend Snippet: MgCl2: Melt® results.

Article Snippet: Each sample consisted of: 50 ng cDNA, 3 mM MgCl2 , 200 μM dNTP, 500 nM of primers, 2 μl of 10X PCR buffer, 0.1 unit of Taq polymerase and SYBR® Green (Molecular Probe, Eugene, OR; 1/30 000 dilution), in a reaction volume of 20 μl.

Techniques:

Journal: Biological Procedures Online

Article Title: Rapid and simple comparison of messenger RNA levels using real-time PCR

doi: 10.1251/bpo114

Figure Lengend Snippet: Optimization of MgCl2 concentration in samples.

Article Snippet: Each sample consisted of: 50 ng cDNA, 3 mM MgCl2 , 200 μM dNTP, 500 nM of primers, 2 μl of 10X PCR buffer, 0.1 unit of Taq polymerase and SYBR® Green (Molecular Probe, Eugene, OR; 1/30 000 dilution), in a reaction volume of 20 μl.

Techniques: Concentration Assay