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  • 99
    New England Biolabs mgcl2
    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM <t>MgCl2</t> (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.
    Mgcl2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mgcl 2
    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM <t>MgCl2</t> (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.
    Mgcl 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 529 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mgcl 2
    Physicochemical characterization of 100 nm PS nanoparticles in calcium solutions. (a) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of aqueous calcium chloride solutions as measured by DLS (n = 3). (b) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of magnesium chloride solutions as measured by DLS (n = 3). All values are reported as mean ± SEM. Significance as compared with 0 mM condition are indicated by * p
    Mgcl 2, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega mgcl 2
    Physicochemical characterization of 100 nm PS nanoparticles in calcium solutions. (a) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of aqueous calcium chloride solutions as measured by DLS (n = 3). (b) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of magnesium chloride solutions as measured by DLS (n = 3). All values are reported as mean ± SEM. Significance as compared with 0 mM condition are indicated by * p
    Mgcl 2, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    PerkinElmer mgcl 2
    Physicochemical characterization of 100 nm PS nanoparticles in calcium solutions. (a) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of aqueous calcium chloride solutions as measured by DLS (n = 3). (b) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of magnesium chloride solutions as measured by DLS (n = 3). All values are reported as mean ± SEM. Significance as compared with 0 mM condition are indicated by * p
    Mgcl 2, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa mgcl 2
    Fluorescence intensity increment versus time of molecular beacon arrays immobilized on ( A ) a glutaraldehyde-derived glass slide and ( B ) an agarose film coated on a glass slide. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0 and 500 mM <t>MgCl2.</t>
    Mgcl 2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche mgcl 2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl 2, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher mgcl 2 solution
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl 2 Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mgcl 2 applied biosystems amplitaq gold dna
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl 2 Applied Biosystems Amplitaq Gold Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad mgcl2
    PCR optimization on mouse testis cDNA by <t>MgCl2</t> gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).
    Mgcl2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 77982 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jena Bioscience mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Amresco mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Amresco, used in various techniques. Bioz Stars score: 94/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    scharlau mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by scharlau, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    EuroClone mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by EuroClone, used in various techniques. Bioz Stars score: 93/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Quality Biological Inc mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Quality Biological Inc, used in various techniques. Bioz Stars score: 90/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Avantor mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Avantor, used in various techniques. Bioz Stars score: 97/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Metabion International AG mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Solis BioDyne mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CinnaGen Co mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by CinnaGen Co, used in various techniques. Bioz Stars score: 93/100, based on 1085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 8697 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 99/100, based on 771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Valiant mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Valiant, used in various techniques. Bioz Stars score: 95/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Applichem mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Applichem, used in various techniques. Bioz Stars score: 97/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mallinckrodt mgcl2
    <t>MgCl2:</t> Melt® results.
    Mgcl2, supplied by Mallinckrodt, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Nacalai mgcl2
    Compaction of T4 DNA and reconstituted chromatin by divalent cation (Mg 2+ ). Changes in the average long-axis length of YOYO-labeled T4 DNA (0.2 μM) and T4 DNA reconstituted with HOs (0.2 μM) at different loading degrees at various concentrations of magnesium chloride (MgCl 2 ) in a bulk solution of TE buffer with 10 mM of KCl. Blue areas correspond to Mg 2+  concentrations at which DNA or chromatin molecules are completely compacted (globule state). The error bars indicate the standard deviations of the average values measured over at least 100 individual DNA or chromatin molecules.
    Mgcl2, supplied by Nacalai, used in various techniques. Bioz Stars score: 99/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Kapa Biosystems mgcl2
    Compaction of T4 DNA and reconstituted chromatin by divalent cation (Mg 2+ ). Changes in the average long-axis length of YOYO-labeled T4 DNA (0.2 μM) and T4 DNA reconstituted with HOs (0.2 μM) at different loading degrees at various concentrations of magnesium chloride (MgCl 2 ) in a bulk solution of TE buffer with 10 mM of KCl. Blue areas correspond to Mg 2+  concentrations at which DNA or chromatin molecules are completely compacted (globule state). The error bars indicate the standard deviations of the average values measured over at least 100 individual DNA or chromatin molecules.
    Mgcl2, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 95/100, based on 796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Fisher Scientific mgcl2
    EGCG binding to the E·(PAP)2 Complex. A.) The Binding-Reaction Progress Curve . Binding-induced fluorescence changes were monitored using a stopped-flow fluorimeter (λ ex = 290 nm, λ em ≥ 325 nm (cutoff filter). Reactions were initiated by rapidly mixing (1:1 v/v) a solution containing SULT1A1 (0.25μM, active sites), PAP (0.50 mM), <t>MgCl2</t> (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C with a solution that was identical except that it lacked SULT1A1 and contained EGCG. The curve through the data (red line) is the behavior predicted by the best fit to a double-exponential model. The insert shows the residuals from a single- (black) and doubleexponential (red) fit. The single-exponential model deviates substantially from the data, while the bi-phasic model provides an excellent fit. B.) k obs vs [EGCG] for the First (Fast) Phase . kobs values were obtained at the four indicated EGCG concentrations under the conditions given in Panel A. Each value is the average of three independent determinations. The reactions were pseudo-first-order in EGCG in all cases. C.) EGCG Dissociation from E·(PAP) 2 ·(EGCG) 2 Complex . A pulse solution containing SULT1A1 (20 μM, monomer), EGCG (24 μM (4.0 μM free, 100 × Kd)), and PAP (500 μM, 16 ▪ K d low affinity site ), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C, was diluted 200:1 into a chase solution containing 75 μM 4-hydroxytamoxifen (TAM, 100 ▪ K d cap open form ), PAP (0.50 mM, 16 ▪ K d low affinity site ), NaPO4 (50 mM), MgCl2 (5.0 mM), pH 7.5, 25 ± 2°C. The final EGCG concentration is 0.12 μM (0.20 ▪ K d cap open form ). Dissociation was monitored by a change in SULT1A1 fluorescence (λ ex = 290 nm, λ em = 370 nm). Three progress curves (black dots) are superposed. The curve through the data is the behavior predicted by a best-fit, single-exponential model.
    Mgcl2, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 97/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Bangalore Genei mgcl2
    EGCG binding to the E·(PAP)2 Complex. A.) The Binding-Reaction Progress Curve . Binding-induced fluorescence changes were monitored using a stopped-flow fluorimeter (λ ex = 290 nm, λ em ≥ 325 nm (cutoff filter). Reactions were initiated by rapidly mixing (1:1 v/v) a solution containing SULT1A1 (0.25μM, active sites), PAP (0.50 mM), <t>MgCl2</t> (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C with a solution that was identical except that it lacked SULT1A1 and contained EGCG. The curve through the data (red line) is the behavior predicted by the best fit to a double-exponential model. The insert shows the residuals from a single- (black) and doubleexponential (red) fit. The single-exponential model deviates substantially from the data, while the bi-phasic model provides an excellent fit. B.) k obs vs [EGCG] for the First (Fast) Phase . kobs values were obtained at the four indicated EGCG concentrations under the conditions given in Panel A. Each value is the average of three independent determinations. The reactions were pseudo-first-order in EGCG in all cases. C.) EGCG Dissociation from E·(PAP) 2 ·(EGCG) 2 Complex . A pulse solution containing SULT1A1 (20 μM, monomer), EGCG (24 μM (4.0 μM free, 100 × Kd)), and PAP (500 μM, 16 ▪ K d low affinity site ), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C, was diluted 200:1 into a chase solution containing 75 μM 4-hydroxytamoxifen (TAM, 100 ▪ K d cap open form ), PAP (0.50 mM, 16 ▪ K d low affinity site ), NaPO4 (50 mM), MgCl2 (5.0 mM), pH 7.5, 25 ± 2°C. The final EGCG concentration is 0.12 μM (0.20 ▪ K d cap open form ). Dissociation was monitored by a change in SULT1A1 fluorescence (λ ex = 290 nm, λ em = 370 nm). Three progress curves (black dots) are superposed. The curve through the data is the behavior predicted by a best-fit, single-exponential model.
    Mgcl2, supplied by Bangalore Genei, used in various techniques. Bioz Stars score: 96/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    BIORON GmbH mgcl2
    EGCG binding to the E·(PAP)2 Complex. A.) The Binding-Reaction Progress Curve . Binding-induced fluorescence changes were monitored using a stopped-flow fluorimeter (λ ex = 290 nm, λ em ≥ 325 nm (cutoff filter). Reactions were initiated by rapidly mixing (1:1 v/v) a solution containing SULT1A1 (0.25μM, active sites), PAP (0.50 mM), <t>MgCl2</t> (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C with a solution that was identical except that it lacked SULT1A1 and contained EGCG. The curve through the data (red line) is the behavior predicted by the best fit to a double-exponential model. The insert shows the residuals from a single- (black) and doubleexponential (red) fit. The single-exponential model deviates substantially from the data, while the bi-phasic model provides an excellent fit. B.) k obs vs [EGCG] for the First (Fast) Phase . kobs values were obtained at the four indicated EGCG concentrations under the conditions given in Panel A. Each value is the average of three independent determinations. The reactions were pseudo-first-order in EGCG in all cases. C.) EGCG Dissociation from E·(PAP) 2 ·(EGCG) 2 Complex . A pulse solution containing SULT1A1 (20 μM, monomer), EGCG (24 μM (4.0 μM free, 100 × Kd)), and PAP (500 μM, 16 ▪ K d low affinity site ), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C, was diluted 200:1 into a chase solution containing 75 μM 4-hydroxytamoxifen (TAM, 100 ▪ K d cap open form ), PAP (0.50 mM, 16 ▪ K d low affinity site ), NaPO4 (50 mM), MgCl2 (5.0 mM), pH 7.5, 25 ± 2°C. The final EGCG concentration is 0.12 μM (0.20 ▪ K d cap open form ). Dissociation was monitored by a change in SULT1A1 fluorescence (λ ex = 290 nm, λ em = 370 nm). Three progress curves (black dots) are superposed. The curve through the data is the behavior predicted by a best-fit, single-exponential model.
    Mgcl2, supplied by BIORON GmbH, used in various techniques. Bioz Stars score: 90/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Journal: Journal of Insect Science

    Article Title: Rapid Identification of Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) Using Ribosomal RNA Internal Transcribed Spacer 1

    doi: 10.1093/jisesa/iev137

    Figure Lengend Snippet: Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Article Snippet: LongAmp Taq polymerase and reaction buffer with 2.5 mM MgCl2 (New England Biolabs) produced dissociation curves with lower intensity than ready-to-use master mixes but sufficient to detect H. armigera in 1:24 ratio of H. armigera : H. zea tissue lysate ( a and b).

    Techniques: Produced, Modification, Amplification

    Physicochemical characterization of 100 nm PS nanoparticles in calcium solutions. (a) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of aqueous calcium chloride solutions as measured by DLS (n = 3). (b) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of magnesium chloride solutions as measured by DLS (n = 3). All values are reported as mean ± SEM. Significance as compared with 0 mM condition are indicated by * p

    Journal: Colloids and surfaces. B, Biointerfaces

    Article Title: Colloidal stability as a determinant of nanoparticle behavior in the brain

    doi: 10.1016/j.colsurfb.2018.06.050

    Figure Lengend Snippet: Physicochemical characterization of 100 nm PS nanoparticles in calcium solutions. (a) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of aqueous calcium chloride solutions as measured by DLS (n = 3). (b) Hydrodynamic diameters of PS-COOH and PS-PEG nanoparticles in varying concentrations of magnesium chloride solutions as measured by DLS (n = 3). All values are reported as mean ± SEM. Significance as compared with 0 mM condition are indicated by * p

    Article Snippet: ACSF was prepared with the addition of the following concentrations of reagents to deionized (DI) water: 119 mM NaCl (MilliporeSigma), 26.2 mM NaHCO3 (MilliporeSigma), 2.5 mM KCl (MilliporeSigma), 1 mM NaH2 PO4 (MilliporeSigma), 1.3 mM MgCl2 (MilliporeSigma), and 10 mM glucose (MilliporeSigma).

    Techniques:

    Fluorescence intensity increment versus time of molecular beacon arrays immobilized on ( A ) a glutaraldehyde-derived glass slide and ( B ) an agarose film coated on a glass slide. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0 and 500 mM MgCl2.

    Journal: Nucleic Acids Research

    Article Title: Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film

    doi:

    Figure Lengend Snippet: Fluorescence intensity increment versus time of molecular beacon arrays immobilized on ( A ) a glutaraldehyde-derived glass slide and ( B ) an agarose film coated on a glass slide. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0 and 500 mM MgCl2.

    Article Snippet: The symmetric PCRs were performed in a total volume of 25 µl containing 75 mM Tris–HCl pH 9.0, 20 mM ammonium sulfate, 0.1 ml/l Tween, 1.5 mM MgCl2 , 500 nM each primer, 200 µM dNTPs, 100 ml/l DMSO and 1 U Taq polymerase (Takara Shuzo Co. Ltd).

    Techniques: Fluorescence, Derivative Assay, Hybridization

    Hybridization dynamics images of molecular beacon arrays immobilized on ( A ) a glutaraldehyde-derived glass slide and ( B ) an agarose film. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0 and 500 mM MgCl2.

    Journal: Nucleic Acids Research

    Article Title: Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film

    doi:

    Figure Lengend Snippet: Hybridization dynamics images of molecular beacon arrays immobilized on ( A ) a glutaraldehyde-derived glass slide and ( B ) an agarose film. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0 and 500 mM MgCl2.

    Article Snippet: The symmetric PCRs were performed in a total volume of 25 µl containing 75 mM Tris–HCl pH 9.0, 20 mM ammonium sulfate, 0.1 ml/l Tween, 1.5 mM MgCl2 , 500 nM each primer, 200 µM dNTPs, 100 ml/l DMSO and 1 U Taq polymerase (Takara Shuzo Co. Ltd).

    Techniques: Hybridization, Derivative Assay

    Mismatch discrimination ratios of the different molecular beacons in bulk solution, immobilized on a glutaraldehyde-derived glass slide and on an agarose film, respectively. Discrimination ratio is defined as (PM – MM)/PM. PM is the fluorescence increment of perfectly matched probes and MM is the average fluorescence increment of three 1 base mismatched probes. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0, with different concentrations of MgCl2.

    Journal: Nucleic Acids Research

    Article Title: Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film

    doi:

    Figure Lengend Snippet: Mismatch discrimination ratios of the different molecular beacons in bulk solution, immobilized on a glutaraldehyde-derived glass slide and on an agarose film, respectively. Discrimination ratio is defined as (PM – MM)/PM. PM is the fluorescence increment of perfectly matched probes and MM is the average fluorescence increment of three 1 base mismatched probes. The hybridization solution was 10 nM target T2 in 20 mM Tris–HCl pH 8.0, with different concentrations of MgCl2.

    Article Snippet: The symmetric PCRs were performed in a total volume of 25 µl containing 75 mM Tris–HCl pH 9.0, 20 mM ammonium sulfate, 0.1 ml/l Tween, 1.5 mM MgCl2 , 500 nM each primer, 200 µM dNTPs, 100 ml/l DMSO and 1 U Taq polymerase (Takara Shuzo Co. Ltd).

    Techniques: Derivative Assay, Fluorescence, Hybridization

    PCR optimization on mouse testis cDNA by MgCl2 gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).

    Journal: Journal of Reproduction & Infertility

    Article Title: Producing Recombinant mTEX101; a Murine Testis Specific Protein

    doi:

    Figure Lengend Snippet: PCR optimization on mouse testis cDNA by MgCl2 gradients. 1-4: MgCl2 concentrations from 1 to 4 mM, respectively, 5: 1 Kb DNA ladder, 6: negative control (no DNA).

    Article Snippet: PCR amplifications for mTEX101 transcript was performed in a volume of 25µl using 10-20ng of testis cDNA, forward and reverse primers (10pmol each), specific for mTEX101 transcript, 10X PCR buffer (2.5µl ), dNTP mixture (0.2mM each), 1mM MgCl2, and 1 unit of Taq DNA polymerase (Roche, Mannheim, Germany).

    Techniques: Polymerase Chain Reaction, Negative Control

    MgCl2: Melt® results.

    Journal: Biological Procedures Online

    Article Title: Rapid and simple comparison of messenger RNA levels using real-time PCR

    doi: 10.1251/bpo114

    Figure Lengend Snippet: MgCl2: Melt® results.

    Article Snippet: Each sample consisted of: 50 ng cDNA, 3 mM MgCl2 , 200 μM dNTP, 500 nM of primers, 2 μl of 10X PCR buffer, 0.1 unit of Taq polymerase and SYBR® Green (Molecular Probe, Eugene, OR; 1/30 000 dilution), in a reaction volume of 20 μl.

    Techniques:

    Optimization of MgCl2 concentration in samples.

    Journal: Biological Procedures Online

    Article Title: Rapid and simple comparison of messenger RNA levels using real-time PCR

    doi: 10.1251/bpo114

    Figure Lengend Snippet: Optimization of MgCl2 concentration in samples.

    Article Snippet: Each sample consisted of: 50 ng cDNA, 3 mM MgCl2 , 200 μM dNTP, 500 nM of primers, 2 μl of 10X PCR buffer, 0.1 unit of Taq polymerase and SYBR® Green (Molecular Probe, Eugene, OR; 1/30 000 dilution), in a reaction volume of 20 μl.

    Techniques: Concentration Assay

    Compaction of T4 DNA and reconstituted chromatin by divalent cation (Mg 2+ ). Changes in the average long-axis length of YOYO-labeled T4 DNA (0.2 μM) and T4 DNA reconstituted with HOs (0.2 μM) at different loading degrees at various concentrations of magnesium chloride (MgCl 2 ) in a bulk solution of TE buffer with 10 mM of KCl. Blue areas correspond to Mg 2+  concentrations at which DNA or chromatin molecules are completely compacted (globule state). The error bars indicate the standard deviations of the average values measured over at least 100 individual DNA or chromatin molecules.

    Journal: Nucleic Acids Research

    Article Title: Single-molecule compaction of megabase-long chromatin molecules by multivalent cations

    doi: 10.1093/nar/gkx1135

    Figure Lengend Snippet: Compaction of T4 DNA and reconstituted chromatin by divalent cation (Mg 2+ ). Changes in the average long-axis length of YOYO-labeled T4 DNA (0.2 μM) and T4 DNA reconstituted with HOs (0.2 μM) at different loading degrees at various concentrations of magnesium chloride (MgCl 2 ) in a bulk solution of TE buffer with 10 mM of KCl. Blue areas correspond to Mg 2+ concentrations at which DNA or chromatin molecules are completely compacted (globule state). The error bars indicate the standard deviations of the average values measured over at least 100 individual DNA or chromatin molecules.

    Article Snippet: NaCl, MgCl2 , and spermine tetrahydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Labeling

    EGCG binding to the E·(PAP)2 Complex. A.) The Binding-Reaction Progress Curve . Binding-induced fluorescence changes were monitored using a stopped-flow fluorimeter (λ ex = 290 nm, λ em ≥ 325 nm (cutoff filter). Reactions were initiated by rapidly mixing (1:1 v/v) a solution containing SULT1A1 (0.25μM, active sites), PAP (0.50 mM), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C with a solution that was identical except that it lacked SULT1A1 and contained EGCG. The curve through the data (red line) is the behavior predicted by the best fit to a double-exponential model. The insert shows the residuals from a single- (black) and doubleexponential (red) fit. The single-exponential model deviates substantially from the data, while the bi-phasic model provides an excellent fit. B.) k obs vs [EGCG] for the First (Fast) Phase . kobs values were obtained at the four indicated EGCG concentrations under the conditions given in Panel A. Each value is the average of three independent determinations. The reactions were pseudo-first-order in EGCG in all cases. C.) EGCG Dissociation from E·(PAP) 2 ·(EGCG) 2 Complex . A pulse solution containing SULT1A1 (20 μM, monomer), EGCG (24 μM (4.0 μM free, 100 × Kd)), and PAP (500 μM, 16 ▪ K d low affinity site ), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C, was diluted 200:1 into a chase solution containing 75 μM 4-hydroxytamoxifen (TAM, 100 ▪ K d cap open form ), PAP (0.50 mM, 16 ▪ K d low affinity site ), NaPO4 (50 mM), MgCl2 (5.0 mM), pH 7.5, 25 ± 2°C. The final EGCG concentration is 0.12 μM (0.20 ▪ K d cap open form ). Dissociation was monitored by a change in SULT1A1 fluorescence (λ ex = 290 nm, λ em = 370 nm). Three progress curves (black dots) are superposed. The curve through the data is the behavior predicted by a best-fit, single-exponential model.

    Journal: Biochemistry

    Article Title: Isozyme Specific Allosteric Regulation of Human Sulfotransferase 1A1

    doi: 10.1021/acs.biochem.6b00401

    Figure Lengend Snippet: EGCG binding to the E·(PAP)2 Complex. A.) The Binding-Reaction Progress Curve . Binding-induced fluorescence changes were monitored using a stopped-flow fluorimeter (λ ex = 290 nm, λ em ≥ 325 nm (cutoff filter). Reactions were initiated by rapidly mixing (1:1 v/v) a solution containing SULT1A1 (0.25μM, active sites), PAP (0.50 mM), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C with a solution that was identical except that it lacked SULT1A1 and contained EGCG. The curve through the data (red line) is the behavior predicted by the best fit to a double-exponential model. The insert shows the residuals from a single- (black) and doubleexponential (red) fit. The single-exponential model deviates substantially from the data, while the bi-phasic model provides an excellent fit. B.) k obs vs [EGCG] for the First (Fast) Phase . kobs values were obtained at the four indicated EGCG concentrations under the conditions given in Panel A. Each value is the average of three independent determinations. The reactions were pseudo-first-order in EGCG in all cases. C.) EGCG Dissociation from E·(PAP) 2 ·(EGCG) 2 Complex . A pulse solution containing SULT1A1 (20 μM, monomer), EGCG (24 μM (4.0 μM free, 100 × Kd)), and PAP (500 μM, 16 ▪ K d low affinity site ), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.5, 25 ± 2°C, was diluted 200:1 into a chase solution containing 75 μM 4-hydroxytamoxifen (TAM, 100 ▪ K d cap open form ), PAP (0.50 mM, 16 ▪ K d low affinity site ), NaPO4 (50 mM), MgCl2 (5.0 mM), pH 7.5, 25 ± 2°C. The final EGCG concentration is 0.12 μM (0.20 ▪ K d cap open form ). Dissociation was monitored by a change in SULT1A1 fluorescence (λ ex = 290 nm, λ em = 370 nm). Three progress curves (black dots) are superposed. The curve through the data is the behavior predicted by a best-fit, single-exponential model.

    Article Snippet: Ampicillin, HEPES, KCl, KOH, MgCl2 , NaCl and phenylmethylsulfonyl fluoride (PMSF) were purchased from Fisher Scientific.

    Techniques: Binding Assay, Fluorescence, Flow Cytometry, Concentration Assay