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  • 99
    Millipore mg2 cl2
    Mg2 Cl2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher mg2 buffer
    Mg2 Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Biochrom mg2 ca2
    Mg2 Ca2, supplied by Biochrom, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Lonza ca2 mg2
    Ca2 Mg2, supplied by Lonza, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    GE Healthcare mg2 ca2
    Mg2 Ca2, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore mg2 so4
    Mg2 So4, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    PAN - Biotech ca2 mg2
    Ca2 Mg2, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher ca2 mg2
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Ca2 Mg2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore ca2 mg2
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Ca2 Mg2, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    FUJIFILM mg2 cl
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg2 Cl, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Fisher Scientific mg2 content
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg2 Content, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 84/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PerkinElmer mg2 buffer
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg2 Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Mediatech mg 2 dpbs
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg 2 Dpbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher dpbs ca2 mg2
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Dpbs Ca2 Mg2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher hbss mg2 ca2
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Hbss Mg2 Ca2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore mg2 atp
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg2 Atp, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega atp mg2
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Atp Mg2, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Lonza mg2 egta
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg2 Egta, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher mg2 free hbss
    40mM <t>Mg2+</t> increases facilitation, but 5 nM ω-Aga IVA does not
    Mg2 Free Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beyotime mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Carl Roth GmbH mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mg2  (Cellgro)
    93
    Cellgro mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Cellgro, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Corning Life Sciences mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 98/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    GE Healthcare mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 71 article reviews
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    88
    GL Sciences mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by GL Sciences, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mg2  (Lonza)
    98
    Lonza mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Lonza, used in various techniques. Bioz Stars score: 98/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Mediatech mg2
    Equilibrium binding of <t>Mg2+</t> to RecBC and RecBC-Reference DNA complex
    Mg2, supplied by Mediatech, used in various techniques. Bioz Stars score: 94/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore mg2
    FPLC gel filtration (size exclusion chromatography) of unstimulated pooled whole saliva. Unstimulated whole saliva was separated by gel filtration chromatography (FPLC) on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm. The seven fractions were tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant <t>MG2,</t> sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.
    Mg2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Novoprotein mg2
    FPLC gel filtration (size exclusion chromatography) of unstimulated pooled whole saliva. Unstimulated whole saliva was separated by gel filtration chromatography (FPLC) on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm. The seven fractions were tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant <t>MG2,</t> sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.
    Mg2, supplied by Novoprotein, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PAA Laboratories mg2
    FPLC gel filtration (size exclusion chromatography) of unstimulated pooled whole saliva. Unstimulated whole saliva was separated by gel filtration chromatography (FPLC) on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm. The seven fractions were tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant <t>MG2,</t> sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.
    Mg2, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    40mM Mg2+ increases facilitation, but 5 nM ω-Aga IVA does not

    Journal:

    Article Title: Effects of increasing Ca2+ channel-vesicle separation on facilitation at the crayfish inhibitory neuromuscular junction

    doi: 10.1016/j.neuroscience.2008.02.045

    Figure Lengend Snippet: 40mM Mg2+ increases facilitation, but 5 nM ω-Aga IVA does not

    Article Snippet: The calibration solution was prepared by dissolving the dye in a standard buffer with a final [Ca2+ ] of 100 nM (Ca2+ Calibration Buffer Kit with 1 mM Mg2+ , Molecular Probes, C3721).

    Techniques:

    Equilibrium binding of Mg2+ to RecBC and RecBC-Reference DNA complex

    Journal:

    Article Title: Kinetic Control of Mg2+-dependent Melting of Duplex DNA Ends by E. coli RecBC

    doi: 10.1016/j.jmb.2008.03.023

    Figure Lengend Snippet: Equilibrium binding of Mg2+ to RecBC and RecBC-Reference DNA complex

    Article Snippet: Expressions for BT , DT and total Mg2+ concentration (MgT ) in terms of Bf , Df , free Mg2+ concentration (Mgf ), K R , K R,no Mg , K Mg B and K Mg BD are given in , and respectively: B T = B f ( 1 + K Mg B Mg f + 2 ( K R K Mg B + K R , no Mg ) D f + 2 B f D f K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (10) D T = D f ( 1 + 2 B f ( K R K Mg B Mg f + K R , no Mg ) + B f 2 K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (11) Mg T = Mg f ( 1 + K Mg B B f ( 1 + 2 K R D f ) + 2 K R , no Mg 2 B f 2 D f ( 1 + K Mg BD Mg f ) ) (12) In our experiment, since all the reference DNA molecules were bound with two molecules of RecBC before addition of Mg2+ , [BD] = [BD]•Mg2+ = 0 and DT = [B2 D] + [B2 D] •Mg2+ + [B2 D] •(Mg2+ )2 .

    Techniques: Binding Assay

    Effects of Mg2+ on the observed enthalpy and heat capacity changes for RecBC binding to DNA ends

    Journal:

    Article Title: Kinetic Control of Mg2+-dependent Melting of Duplex DNA Ends by E. coli RecBC

    doi: 10.1016/j.jmb.2008.03.023

    Figure Lengend Snippet: Effects of Mg2+ on the observed enthalpy and heat capacity changes for RecBC binding to DNA ends

    Article Snippet: Expressions for BT , DT and total Mg2+ concentration (MgT ) in terms of Bf , Df , free Mg2+ concentration (Mgf ), K R , K R,no Mg , K Mg B and K Mg BD are given in , and respectively: B T = B f ( 1 + K Mg B Mg f + 2 ( K R K Mg B + K R , no Mg ) D f + 2 B f D f K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (10) D T = D f ( 1 + 2 B f ( K R K Mg B Mg f + K R , no Mg ) + B f 2 K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (11) Mg T = Mg f ( 1 + K Mg B B f ( 1 + 2 K R D f ) + 2 K R , no Mg 2 B f 2 D f ( 1 + K Mg BD Mg f ) ) (12) In our experiment, since all the reference DNA molecules were bound with two molecules of RecBC before addition of Mg2+ , [BD] = [BD]•Mg2+ = 0 and DT = [B2 D] + [B2 D] •Mg2+ + [B2 D] •(Mg2+ )2 .

    Techniques: Binding Assay

    The pre-existing ss-(dT)n tail length influence the effects of Mg2+ on RecBC binding to DNA ends

    Journal:

    Article Title: Kinetic Control of Mg2+-dependent Melting of Duplex DNA Ends by E. coli RecBC

    doi: 10.1016/j.jmb.2008.03.023

    Figure Lengend Snippet: The pre-existing ss-(dT)n tail length influence the effects of Mg2+ on RecBC binding to DNA ends

    Article Snippet: Expressions for BT , DT and total Mg2+ concentration (MgT ) in terms of Bf , Df , free Mg2+ concentration (Mgf ), K R , K R,no Mg , K Mg B and K Mg BD are given in , and respectively: B T = B f ( 1 + K Mg B Mg f + 2 ( K R K Mg B + K R , no Mg ) D f + 2 B f D f K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (10) D T = D f ( 1 + 2 B f ( K R K Mg B Mg f + K R , no Mg ) + B f 2 K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (11) Mg T = Mg f ( 1 + K Mg B B f ( 1 + 2 K R D f ) + 2 K R , no Mg 2 B f 2 D f ( 1 + K Mg BD Mg f ) ) (12) In our experiment, since all the reference DNA molecules were bound with two molecules of RecBC before addition of Mg2+ , [BD] = [BD]•Mg2+ = 0 and DT = [B2 D] + [B2 D] •Mg2+ + [B2 D] •(Mg2+ )2 .

    Techniques: Binding Assay

    Effects of Mg2+ on the observed enthalpy and heat capacity changes for RecBC binding to DNA ends

    Journal:

    Article Title: Kinetic Control of Mg2+-dependent Melting of Duplex DNA Ends by E. coli RecBC

    doi: 10.1016/j.jmb.2008.03.023

    Figure Lengend Snippet: Effects of Mg2+ on the observed enthalpy and heat capacity changes for RecBC binding to DNA ends

    Article Snippet: Expressions for BT , DT and total Mg2+ concentration (MgT ) in terms of Bf , Df , free Mg2+ concentration (Mgf ), K R , K R,no Mg , K Mg B and K Mg BD are given in , and respectively: B T = B f ( 1 + K Mg B Mg f + 2 ( K R K Mg B + K R , no Mg ) D f + 2 B f D f K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (10) D T = D f ( 1 + 2 B f ( K R K Mg B Mg f + K R , no Mg ) + B f 2 K R , no Mg 2 ( 1 + K Mg BD Mg f ) 2 ) (11) Mg T = Mg f ( 1 + K Mg B B f ( 1 + 2 K R D f ) + 2 K R , no Mg 2 B f 2 D f ( 1 + K Mg BD Mg f ) ) (12) In our experiment, since all the reference DNA molecules were bound with two molecules of RecBC before addition of Mg2+ , [BD] = [BD]•Mg2+ = 0 and DT = [B2 D] + [B2 D] •Mg2+ + [B2 D] •(Mg2+ )2 .

    Techniques: Binding Assay

    FPLC gel filtration (size exclusion chromatography) of unstimulated pooled whole saliva. Unstimulated whole saliva was separated by gel filtration chromatography (FPLC) on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm. The seven fractions were tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant MG2, sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.

    Journal: Clinical and Vaccine Immunology

    Article Title: Comparison of Human Immunodeficiency Virus Type 1-Specific Inhibitory Activities in Saliva and Other Human Mucosal Fluids ▿

    doi: 10.1128/CDLI.00426-05

    Figure Lengend Snippet: FPLC gel filtration (size exclusion chromatography) of unstimulated pooled whole saliva. Unstimulated whole saliva was separated by gel filtration chromatography (FPLC) on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm. The seven fractions were tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant MG2, sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.

    Article Snippet: The positions of the marker proteins, MG2 (250 kDa; Sigma), sheep breast milk lactoferrin (80 kDa), and recombinant SLPI (14.7 kDa; R & D Systems, United Kingdom), were calibrated on a Superose 12 gel chromatography column (Pharmacia).

    Techniques: Fast Protein Liquid Chromatography, Filtration, Size-exclusion Chromatography, Chromatography, Activity Assay, Marker, Recombinant

    Effect of antilactoferrin, anti-SLPI, and anti-MG2 antibodies on HIV-1 inhibitory activity of pooled whole saliva from four donors. Pooled, filtered whole saliva was immunoprecipitated with goat polyclonal anti-SLPI, sheep polyclonal antilactoferrin, anti-MG2, or control IgG antibodies for 1 h at 37°C in 5% CO 2 prior to the addition of C8166 T cells. Samples were assessed for depletion in HIV inhibitory activity by quantification of p24 antigen (p24Ag) after 72 h using the 3-day microtiter assay. All samples were assayed in triplicate in three independent assays, and the means ± standard errors of the means are shown. V/C, virus control; WS, whole saliva; Control IgG, control antibody; hLf Ab, antilactoferrin antibody; SLPI Ab, SLPI antibody; MUC7 Ab, MG2 antibody. ♦, significantly different from the IgG control ( P

    Journal: Clinical and Vaccine Immunology

    Article Title: Comparison of Human Immunodeficiency Virus Type 1-Specific Inhibitory Activities in Saliva and Other Human Mucosal Fluids ▿

    doi: 10.1128/CDLI.00426-05

    Figure Lengend Snippet: Effect of antilactoferrin, anti-SLPI, and anti-MG2 antibodies on HIV-1 inhibitory activity of pooled whole saliva from four donors. Pooled, filtered whole saliva was immunoprecipitated with goat polyclonal anti-SLPI, sheep polyclonal antilactoferrin, anti-MG2, or control IgG antibodies for 1 h at 37°C in 5% CO 2 prior to the addition of C8166 T cells. Samples were assessed for depletion in HIV inhibitory activity by quantification of p24 antigen (p24Ag) after 72 h using the 3-day microtiter assay. All samples were assayed in triplicate in three independent assays, and the means ± standard errors of the means are shown. V/C, virus control; WS, whole saliva; Control IgG, control antibody; hLf Ab, antilactoferrin antibody; SLPI Ab, SLPI antibody; MUC7 Ab, MG2 antibody. ♦, significantly different from the IgG control ( P

    Article Snippet: The positions of the marker proteins, MG2 (250 kDa; Sigma), sheep breast milk lactoferrin (80 kDa), and recombinant SLPI (14.7 kDa; R & D Systems, United Kingdom), were calibrated on a Superose 12 gel chromatography column (Pharmacia).

    Techniques: Activity Assay, Immunoprecipitation

    FPLC gel filtration chromatography of pooled breast milk. Pooled breast milk was separated by FPLC gel filtration chromatography on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm, and then divided into nine main fractions. Each fraction was tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant MG2, sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.

    Journal: Clinical and Vaccine Immunology

    Article Title: Comparison of Human Immunodeficiency Virus Type 1-Specific Inhibitory Activities in Saliva and Other Human Mucosal Fluids ▿

    doi: 10.1128/CDLI.00426-05

    Figure Lengend Snippet: FPLC gel filtration chromatography of pooled breast milk. Pooled breast milk was separated by FPLC gel filtration chromatography on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm, and then divided into nine main fractions. Each fraction was tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant MG2, sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.

    Article Snippet: The positions of the marker proteins, MG2 (250 kDa; Sigma), sheep breast milk lactoferrin (80 kDa), and recombinant SLPI (14.7 kDa; R & D Systems, United Kingdom), were calibrated on a Superose 12 gel chromatography column (Pharmacia).

    Techniques: Fast Protein Liquid Chromatography, Filtration, Chromatography, Activity Assay, Marker, Recombinant

    FPLC gel filtration whole-saliva fractions (WS) incubated with anti-MG2 (MUC7), -Lf (LF), and -SLPI (SLPI) and control (IgG) antibodies (AB) to determine the reduction in HIV-1 inhibitory activity. Whole-saliva gel filtration fractions (1 to 7) were incubated with control antibody (IgG), anti-MG2, anti-hLf, or goat polyclonal anti-SLPI antibody for 1 h at 37°C in 5% CO 2 prior to the addition of C8166 cells. Plates were then left for 72 h at 37°C in 5% CO 2 and then assessed for HIV inhibition by quantification of p24 antigen (p24Ag). Samples were assessed in triplicate in three independent assays, and the means ± standard errors of the means are shown. V/C, virus control; ♦, significantly different from the IgG control ( P

    Journal: Clinical and Vaccine Immunology

    Article Title: Comparison of Human Immunodeficiency Virus Type 1-Specific Inhibitory Activities in Saliva and Other Human Mucosal Fluids ▿

    doi: 10.1128/CDLI.00426-05

    Figure Lengend Snippet: FPLC gel filtration whole-saliva fractions (WS) incubated with anti-MG2 (MUC7), -Lf (LF), and -SLPI (SLPI) and control (IgG) antibodies (AB) to determine the reduction in HIV-1 inhibitory activity. Whole-saliva gel filtration fractions (1 to 7) were incubated with control antibody (IgG), anti-MG2, anti-hLf, or goat polyclonal anti-SLPI antibody for 1 h at 37°C in 5% CO 2 prior to the addition of C8166 cells. Plates were then left for 72 h at 37°C in 5% CO 2 and then assessed for HIV inhibition by quantification of p24 antigen (p24Ag). Samples were assessed in triplicate in three independent assays, and the means ± standard errors of the means are shown. V/C, virus control; ♦, significantly different from the IgG control ( P

    Article Snippet: The positions of the marker proteins, MG2 (250 kDa; Sigma), sheep breast milk lactoferrin (80 kDa), and recombinant SLPI (14.7 kDa; R & D Systems, United Kingdom), were calibrated on a Superose 12 gel chromatography column (Pharmacia).

    Techniques: Fast Protein Liquid Chromatography, Filtration, Incubation, Activity Assay, Inhibition