Journal: Clinical and Vaccine Immunology
Article Title: Comparison of Human Immunodeficiency Virus Type 1-Specific Inhibitory Activities in Saliva and Other Human Mucosal Fluids ▿
Figure Lengend Snippet: Effect of antilactoferrin, anti-SLPI, and anti-MG2 antibodies on HIV-1 inhibitory activity of pooled whole saliva from four donors. Pooled, filtered whole saliva was immunoprecipitated with goat polyclonal anti-SLPI, sheep polyclonal antilactoferrin, anti-MG2, or control IgG antibodies for 1 h at 37°C in 5% CO 2 prior to the addition of C8166 T cells. Samples were assessed for depletion in HIV inhibitory activity by quantification of p24 antigen (p24Ag) after 72 h using the 3-day microtiter assay. All samples were assayed in triplicate in three independent assays, and the means ± standard errors of the means are shown. V/C, virus control; WS, whole saliva; Control IgG, control antibody; hLf Ab, antilactoferrin antibody; SLPI Ab, SLPI antibody; MUC7 Ab, MG2 antibody. ♦, significantly different from the IgG control ( P
Article Snippet: The positions of the marker proteins, MG2 (250 kDa; Sigma), sheep breast milk lactoferrin (80 kDa), and recombinant SLPI (14.7 kDa; R & D Systems, United Kingdom), were calibrated on a Superose 12 gel chromatography column (Pharmacia).
Techniques: Activity Assay, Immunoprecipitation