methanol meoh Search Results


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  • 99
    Millipore methanol meoh
    Synthesis of novel 1 H -imidazo-[4,5- c ]quinolin-4-amine derivatives with structural variation at the 4 position. a a Reagents: (i) m -CPBA, CHCl 3 /CH 2 Cl 2 <t>/MeOH;</t> (ii) POCl 3 , <t>toluene/DMF;</t> (iii) R-PhNH 2 , DMF.
    Methanol Meoh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor methanol meoh
    Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) <t>ACN/</t> <t>MeOH/TFA/TEA</t> (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.
    Methanol Meoh, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Burdick & Jackson methanol meoh
    Comparison of separate lipid species from SchuS4 for anti-inflammatory activity. a Separation of crude SchuS4 lipids by TLC. Increasing concentrations of SchuS4 lipids were spotted onto silica plates and separated using ethyl acetate: <t>MeOH</t> (3: 2) and <t>Chl:</t> MeOH:H 2 O (7: 3: 0.25) into 4 distinct bands. b Each band (1–4) was scraped from the silica plate, processed to remove contaminating silica, and added to human dendritic cells (hDC) at 2 µg/mL (3–4 wells/group), followed by stimulation with E. coli LPS (5 ng/mL). Control samples for bands consisted of scrapings from the silica plate adjacent to each sample band. hDC were also treated with 10 µg/mL of crude lipid (CL). Control for crude lipid consisted of EtOH diluted similarly to the lipid. After 24 h, supernatants were assessed for IL-12p40 and TNF-α. Values from the control sample and corresponding band were analyzed using an unpaired, two-tailed, Student's t test. A–C indicate individual donors. Error bars represent standard deviation (SD).
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    EM Science Inc methanol meoh
    Comparison of separate lipid species from SchuS4 for anti-inflammatory activity. a Separation of crude SchuS4 lipids by TLC. Increasing concentrations of SchuS4 lipids were spotted onto silica plates and separated using ethyl acetate: <t>MeOH</t> (3: 2) and <t>Chl:</t> MeOH:H 2 O (7: 3: 0.25) into 4 distinct bands. b Each band (1–4) was scraped from the silica plate, processed to remove contaminating silica, and added to human dendritic cells (hDC) at 2 µg/mL (3–4 wells/group), followed by stimulation with E. coli LPS (5 ng/mL). Control samples for bands consisted of scrapings from the silica plate adjacent to each sample band. hDC were also treated with 10 µg/mL of crude lipid (CL). Control for crude lipid consisted of EtOH diluted similarly to the lipid. After 24 h, supernatants were assessed for IL-12p40 and TNF-α. Values from the control sample and corresponding band were analyzed using an unpaired, two-tailed, Student's t test. A–C indicate individual donors. Error bars represent standard deviation (SD).
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    92
    Fisher Scientific methanol meoh
    Chromatographic fractionation of <t>DCM</t> and EtOAc extract of D. suffruticosa roots. D/F4 and D/F5 represent the active fractions obtained from fractionation of DCM extract using hexane: EtOAc gradient as mobile phase. EA/P2 represents the active fraction obtained from fractionation of EtOAc extract using hexane: EtOAc: <t>MeOH</t> as mobile phase.
    Methanol Meoh, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co methanol meoh
    Chromatographic fractionation of <t>DCM</t> and EtOAc extract of D. suffruticosa roots. D/F4 and D/F5 represent the active fractions obtained from fractionation of DCM extract using hexane: EtOAc gradient as mobile phase. EA/P2 represents the active fraction obtained from fractionation of EtOAc extract using hexane: EtOAc: <t>MeOH</t> as mobile phase.
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    93
    Merck KGaA methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Titan Scientific methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by Shanghai Titan Scientific, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    scharlau methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by scharlau, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biosolve methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by Biosolve, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    honeywell international methanol meoh
    Reducing potential of Ganoderma lucidum extracts at various concentrations. <t>MeOH,</t> methanol; <t>EtOH,</t> ethanol; EtAC, ethylacetae.
    Methanol Meoh, supplied by honeywell international, used in various techniques. Bioz Stars score: 91/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tedia methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by Tedia, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carlo Erba Reagents methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by Carlo Erba Reagents, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dikma Technologies methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by Dikma Technologies, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kanto Chemical methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Burdick & Jackson chromatographic grade methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Chromatographic Grade Methanol Meoh, supplied by Burdick & Jackson, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ERBA Diagnostics methanol meoh carlo erba
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh Carlo Erba, supplied by ERBA Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PHARMCO-AAPER methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by PHARMCO-AAPER, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Scharlab methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Methanol Meoh, supplied by Scharlab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    honeywell international hplc grade methanol meoh
    Comparison of acetonitrile <t>(ACN)</t> and methanol <t>(MeOH)</t> solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P
    Hplc Grade Methanol Meoh, supplied by honeywell international, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Synthesis of novel 1 H -imidazo-[4,5- c ]quinolin-4-amine derivatives with structural variation at the 4 position. a a Reagents: (i) m -CPBA, CHCl 3 /CH 2 Cl 2 /MeOH; (ii) POCl 3 , toluene/DMF; (iii) R-PhNH 2 , DMF.

    Journal: Journal of medicinal chemistry

    Article Title: Novel 2- and 4- Substituted 1H-Imidazo[4,5-c]quinolin-4-amine Derivatives as Allosteric Modulators of the A3 Adenosine Receptor

    doi: 10.1021/jm801659w

    Figure Lengend Snippet: Synthesis of novel 1 H -imidazo-[4,5- c ]quinolin-4-amine derivatives with structural variation at the 4 position. a a Reagents: (i) m -CPBA, CHCl 3 /CH 2 Cl 2 /MeOH; (ii) POCl 3 , toluene/DMF; (iii) R-PhNH 2 , DMF.

    Article Snippet: Suppliers of some commercial compounds are listed as follows: 3,4-diaminoquinoline ( 30 ) was purchased from Tyger Scientific, Inc.; 3,5-bis(trifluoromethyl)aniline and 4-aminophthalonitrile were purchased from Acros Organics; polyphosphoric acid, m -CPBA, phosphorus oxychloride (POCl3 ), chloroform (CHCl3 ), methylene chloride (CH2 Cl2 ), DMF, toluene, diisopropyl ether, methanol (MeOH), tetrahydrofuran (THF), and most of other reagents and solvents were purchased from Sigma-Aldrich; dimethyl sulfoxide (DMSO)- d 6 , chloroform- d (CDCl3 ), and CD3 OD were purchased from Cambridge Isotope Laboratories.

    Techniques:

    Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) ACN/ MeOH/TFA/TEA (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Screening primary racemic amines for enantioseparation by derivatized polysaccharide and cyclofructan columns

    doi: 10.1016/j.jpha.2016.07.003

    Figure Lengend Snippet: Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) ACN/ MeOH/TFA/TEA (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.

    Article Snippet: Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received.

    Techniques: Flow Cytometry

    Separation of the enantiomers of (±) trans-1-Amino-2-indanol (Solute #21 in Table 1 ) on a 25 cm column in PO mode as (A) ChiralPak IB column with ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with ACN/MeOH (90:10, v/v) mobile phase with 0.3% TFA and 0.2% TEA ionic additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Screening primary racemic amines for enantioseparation by derivatized polysaccharide and cyclofructan columns

    doi: 10.1016/j.jpha.2016.07.003

    Figure Lengend Snippet: Separation of the enantiomers of (±) trans-1-Amino-2-indanol (Solute #21 in Table 1 ) on a 25 cm column in PO mode as (A) ChiralPak IB column with ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with ACN/MeOH (90:10, v/v) mobile phase with 0.3% TFA and 0.2% TEA ionic additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Article Snippet: Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received.

    Techniques: Indirect Immunoperoxidase Assay, Flow Cytometry

    Separation of the RS and SR enantiomers of phenylpropanolamine (Solute #26) on a 25 cm column in PO mode as (A) ChiralPak IE column with a ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with a ACN/MeOH (90:10, v/v) mobile phase with 0.3%TFA and 0.2%TEA additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Screening primary racemic amines for enantioseparation by derivatized polysaccharide and cyclofructan columns

    doi: 10.1016/j.jpha.2016.07.003

    Figure Lengend Snippet: Separation of the RS and SR enantiomers of phenylpropanolamine (Solute #26) on a 25 cm column in PO mode as (A) ChiralPak IE column with a ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with a ACN/MeOH (90:10, v/v) mobile phase with 0.3%TFA and 0.2%TEA additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Article Snippet: Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received.

    Techniques: Indirect Immunoperoxidase Assay, Flow Cytometry

    Comparison of separate lipid species from SchuS4 for anti-inflammatory activity. a Separation of crude SchuS4 lipids by TLC. Increasing concentrations of SchuS4 lipids were spotted onto silica plates and separated using ethyl acetate: MeOH (3: 2) and Chl: MeOH:H 2 O (7: 3: 0.25) into 4 distinct bands. b Each band (1–4) was scraped from the silica plate, processed to remove contaminating silica, and added to human dendritic cells (hDC) at 2 µg/mL (3–4 wells/group), followed by stimulation with E. coli LPS (5 ng/mL). Control samples for bands consisted of scrapings from the silica plate adjacent to each sample band. hDC were also treated with 10 µg/mL of crude lipid (CL). Control for crude lipid consisted of EtOH diluted similarly to the lipid. After 24 h, supernatants were assessed for IL-12p40 and TNF-α. Values from the control sample and corresponding band were analyzed using an unpaired, two-tailed, Student's t test. A–C indicate individual donors. Error bars represent standard deviation (SD).

    Journal: Journal of Innate Immunity

    Article Title: Unique Francisella Phosphatidylethanolamine Acts as a Potent Anti-Inflammatory Lipid

    doi: 10.1159/000489504

    Figure Lengend Snippet: Comparison of separate lipid species from SchuS4 for anti-inflammatory activity. a Separation of crude SchuS4 lipids by TLC. Increasing concentrations of SchuS4 lipids were spotted onto silica plates and separated using ethyl acetate: MeOH (3: 2) and Chl: MeOH:H 2 O (7: 3: 0.25) into 4 distinct bands. b Each band (1–4) was scraped from the silica plate, processed to remove contaminating silica, and added to human dendritic cells (hDC) at 2 µg/mL (3–4 wells/group), followed by stimulation with E. coli LPS (5 ng/mL). Control samples for bands consisted of scrapings from the silica plate adjacent to each sample band. hDC were also treated with 10 µg/mL of crude lipid (CL). Control for crude lipid consisted of EtOH diluted similarly to the lipid. After 24 h, supernatants were assessed for IL-12p40 and TNF-α. Values from the control sample and corresponding band were analyzed using an unpaired, two-tailed, Student's t test. A–C indicate individual donors. Error bars represent standard deviation (SD).

    Article Snippet: Primuline, ninhydrin, copper sulfate, isopropanol, acetone, hexane, ethyl acetate, and triglyceride standards were from Sigma-Aldrich, chloroform (Chl) from Macron (Center Valley, PA, USA), and methanol (MeOH) from Burdick Jackson (Muskegon, MI, USA).

    Techniques: Activity Assay, Thin Layer Chromatography, Two Tailed Test, Standard Deviation

    Phosphatidylethanolamine (PE) and phosphatidylcholine (PC) isolated from SchuS4 impair inflammatory responses in human dendritic cells (hDC). Total lipid from SchuS4 was fractionated into 3 classes (fractions I–III [FI–III]) using aminopropyl silica solid-phase extraction, and these were tested for their ability to inhibit cytokine production in hDC. a Each fraction (20 µg/mL) or crude lipid (2 µg/mL) was added to hDC (3–4 wells/group) and incubated overnight. Control samples were treated with EtOH diluted similarly to lipids. hDC were then stimulated with E. coli LPS (5 ng/mL), and supernatants were collected the next day to assess TNF-α and IL-12p40. A–C indicate individual donors. Data were analyzed using one-way-ANOVA followed by Tukey's multiple-comparisons analysis. Error bars represent SD. b Each fraction was analyzed for lipid content by thin-layer chromatography. The indicated lipid fractions were spotted onto a silica plate and developed with Chl: MeOH:H 2 O (7: 3: 025). Lipid standards (Std), consisting of lyso-PC (a), PC (b), lyso-PE (c), and PE (d) were also added to the plate. Bands were visualized using primuline and UV illumination. The arrow designated as (1) indicates the presence of PE and (2) designates PC in FIII. c FIII was further fractionated by silica solid-phase extraction into 2 fractions, enriched for either PE (silica fraction [SF]1) or PC (SF2). SF1, SF2 (at the indicated concentrations), and crude lipid (10 µg/mL) were incubated with hDC overnight, the cells were then stimulated with E. coli LPS (5 ng/mL), and supernatants collected the next day to assess TNF-α and IL-12p40. Mock, mock-treated control. A–C indicate individual donors. Error bars represent SD.

    Journal: Journal of Innate Immunity

    Article Title: Unique Francisella Phosphatidylethanolamine Acts as a Potent Anti-Inflammatory Lipid

    doi: 10.1159/000489504

    Figure Lengend Snippet: Phosphatidylethanolamine (PE) and phosphatidylcholine (PC) isolated from SchuS4 impair inflammatory responses in human dendritic cells (hDC). Total lipid from SchuS4 was fractionated into 3 classes (fractions I–III [FI–III]) using aminopropyl silica solid-phase extraction, and these were tested for their ability to inhibit cytokine production in hDC. a Each fraction (20 µg/mL) or crude lipid (2 µg/mL) was added to hDC (3–4 wells/group) and incubated overnight. Control samples were treated with EtOH diluted similarly to lipids. hDC were then stimulated with E. coli LPS (5 ng/mL), and supernatants were collected the next day to assess TNF-α and IL-12p40. A–C indicate individual donors. Data were analyzed using one-way-ANOVA followed by Tukey's multiple-comparisons analysis. Error bars represent SD. b Each fraction was analyzed for lipid content by thin-layer chromatography. The indicated lipid fractions were spotted onto a silica plate and developed with Chl: MeOH:H 2 O (7: 3: 025). Lipid standards (Std), consisting of lyso-PC (a), PC (b), lyso-PE (c), and PE (d) were also added to the plate. Bands were visualized using primuline and UV illumination. The arrow designated as (1) indicates the presence of PE and (2) designates PC in FIII. c FIII was further fractionated by silica solid-phase extraction into 2 fractions, enriched for either PE (silica fraction [SF]1) or PC (SF2). SF1, SF2 (at the indicated concentrations), and crude lipid (10 µg/mL) were incubated with hDC overnight, the cells were then stimulated with E. coli LPS (5 ng/mL), and supernatants collected the next day to assess TNF-α and IL-12p40. Mock, mock-treated control. A–C indicate individual donors. Error bars represent SD.

    Article Snippet: Primuline, ninhydrin, copper sulfate, isopropanol, acetone, hexane, ethyl acetate, and triglyceride standards were from Sigma-Aldrich, chloroform (Chl) from Macron (Center Valley, PA, USA), and methanol (MeOH) from Burdick Jackson (Muskegon, MI, USA).

    Techniques: Isolation, Incubation, Thin Layer Chromatography

    Phosphatidylcholine (PC) is not required for SchuS4 lipid-mediated inhibition of inflammation in mammalian cells. a Confirmation of absence of PC in SchuS4Δ pc s mutant. Lipids from wild-type (SchuS4) and SchuS4Δ pcs mutant were spotted onto silica plates at increasing concentrations, and developed with Chl: MeOH:H 2 O (7: 3: 0.25). Bands were visualized using primuline and UV illumination. Crude lipids from SchuS4 or SchuS4Δ pcs were added at the indicated concentrations to mouse bone marrow-derived macrophages (BMDM) ( b ) or human dendritic cells (hDC) ( c ) (3–4 wells/group), followed by the addition of R848 (5 ng/mL) or LPS (5 ng/mL), respectively. Mock-treated controls (–; Mock) were treated with EtOH diluted similarly to wells containing 1 µg/mL lipid. Supernatants were assessed for IL-12p40 by ELISA. Data were analyzed using one-way-ANOVA followed by Tukey's multiple-comparisons analysis. BMDM data represents 3 experiments of similar design. A–C indicate individual donors. Error bars represent SD.

    Journal: Journal of Innate Immunity

    Article Title: Unique Francisella Phosphatidylethanolamine Acts as a Potent Anti-Inflammatory Lipid

    doi: 10.1159/000489504

    Figure Lengend Snippet: Phosphatidylcholine (PC) is not required for SchuS4 lipid-mediated inhibition of inflammation in mammalian cells. a Confirmation of absence of PC in SchuS4Δ pc s mutant. Lipids from wild-type (SchuS4) and SchuS4Δ pcs mutant were spotted onto silica plates at increasing concentrations, and developed with Chl: MeOH:H 2 O (7: 3: 0.25). Bands were visualized using primuline and UV illumination. Crude lipids from SchuS4 or SchuS4Δ pcs were added at the indicated concentrations to mouse bone marrow-derived macrophages (BMDM) ( b ) or human dendritic cells (hDC) ( c ) (3–4 wells/group), followed by the addition of R848 (5 ng/mL) or LPS (5 ng/mL), respectively. Mock-treated controls (–; Mock) were treated with EtOH diluted similarly to wells containing 1 µg/mL lipid. Supernatants were assessed for IL-12p40 by ELISA. Data were analyzed using one-way-ANOVA followed by Tukey's multiple-comparisons analysis. BMDM data represents 3 experiments of similar design. A–C indicate individual donors. Error bars represent SD.

    Article Snippet: Primuline, ninhydrin, copper sulfate, isopropanol, acetone, hexane, ethyl acetate, and triglyceride standards were from Sigma-Aldrich, chloroform (Chl) from Macron (Center Valley, PA, USA), and methanol (MeOH) from Burdick Jackson (Muskegon, MI, USA).

    Techniques: Inhibition, Mutagenesis, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Chromatographic fractionation of DCM and EtOAc extract of D. suffruticosa roots. D/F4 and D/F5 represent the active fractions obtained from fractionation of DCM extract using hexane: EtOAc gradient as mobile phase. EA/P2 represents the active fraction obtained from fractionation of EtOAc extract using hexane: EtOAc: MeOH as mobile phase.

    Journal: Molecules

    Article Title: Dillenia Suffruticosa Extract Inhibits Proliferation of Human Breast Cancer Cell Lines (MCF-7 and MDA-MB-231) via Induction of G2/M Arrest and Apoptosis

    doi: 10.3390/molecules181113320

    Figure Lengend Snippet: Chromatographic fractionation of DCM and EtOAc extract of D. suffruticosa roots. D/F4 and D/F5 represent the active fractions obtained from fractionation of DCM extract using hexane: EtOAc gradient as mobile phase. EA/P2 represents the active fraction obtained from fractionation of EtOAc extract using hexane: EtOAc: MeOH as mobile phase.

    Article Snippet: Chemicals Hexane, dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH), dimethyl sulfoxide (DMSO), ethanol and sulfuric acid were purchased from Fisher Scientific (Loughborough, Leicestershire, UK); 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), RNase A, ferric (III) chloride, phosphate-buffered saline (PBS), trypan blue, propidium iodide (PI) were purchased from Sigma–Aldrich Co. (St. Louis, MO, USA); silica gel 60, 0.063–0.200 mm and thin layer chromatography (TLC) silica gel 60 F254 plates were purchased from Merck (Darmstadt, Germany); RPMI 1640, MycoplexTM fetal bovine serum (FBS), penicillin and streptomycin (100x), and trypsin EDTA (1×) were purchased from PAA Laboratories GmBH (Pasching, Austria); Annexin V–FITC Apoptosis Detection Kit I was purchased from BD Biosciences Pharmingen (Franklin Lakes, NJ, USA).

    Techniques: Fractionation

    Reducing potential of Ganoderma lucidum extracts at various concentrations. MeOH, methanol; EtOH, ethanol; EtAC, ethylacetae.

    Journal: Preventive Nutrition and Food Science

    Article Title: Wild Mushrooms: A Potential Source of Nutritional and Antioxidant Attributes with Acceptable Toxicity

    doi: 10.3746/pnf.2017.22.2.124

    Figure Lengend Snippet: Reducing potential of Ganoderma lucidum extracts at various concentrations. MeOH, methanol; EtOH, ethanol; EtAC, ethylacetae.

    Article Snippet: Solvents employed in present research i.e . methanol (MeOH), ethanol (EtOH), ethyl acetate (EtAC), and n- hexane were of analytical grade and supplied by Merck (Darmstadt, Germany).

    Techniques:

    DPPH radical scavenging activity (IC 50 ) of various extracts of Ganoderma lucidum . MeOH, methanol; EtOH, ethanol; EtAC, ethylacetae. Different letters (a–d) above the bars vary significantly ( P

    Journal: Preventive Nutrition and Food Science

    Article Title: Wild Mushrooms: A Potential Source of Nutritional and Antioxidant Attributes with Acceptable Toxicity

    doi: 10.3746/pnf.2017.22.2.124

    Figure Lengend Snippet: DPPH radical scavenging activity (IC 50 ) of various extracts of Ganoderma lucidum . MeOH, methanol; EtOH, ethanol; EtAC, ethylacetae. Different letters (a–d) above the bars vary significantly ( P

    Article Snippet: Solvents employed in present research i.e . methanol (MeOH), ethanol (EtOH), ethyl acetate (EtAC), and n- hexane were of analytical grade and supplied by Merck (Darmstadt, Germany).

    Techniques: Activity Assay

    Anti-hemolytic activity of various extracts of wild Ganoderma lucidum . MeOH, methanol; EtOH, ethanol; EtAC, ethylacetate; control 1, Triton X-100; control 2, potassium-buffered saline. Different letters (a–d) above the bars vary significantly ( P

    Journal: Preventive Nutrition and Food Science

    Article Title: Wild Mushrooms: A Potential Source of Nutritional and Antioxidant Attributes with Acceptable Toxicity

    doi: 10.3746/pnf.2017.22.2.124

    Figure Lengend Snippet: Anti-hemolytic activity of various extracts of wild Ganoderma lucidum . MeOH, methanol; EtOH, ethanol; EtAC, ethylacetate; control 1, Triton X-100; control 2, potassium-buffered saline. Different letters (a–d) above the bars vary significantly ( P

    Article Snippet: Solvents employed in present research i.e . methanol (MeOH), ethanol (EtOH), ethyl acetate (EtAC), and n- hexane were of analytical grade and supplied by Merck (Darmstadt, Germany).

    Techniques: Activity Assay

    Comparison of acetonitrile (ACN) and methanol (MeOH) solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P

    Journal: Frontiers in Plant Science

    Article Title: A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

    doi: 10.3389/fpls.2017.00064

    Figure Lengend Snippet: Comparison of acetonitrile (ACN) and methanol (MeOH) solvent on the extraction efficiencies and matrix effects of MLT. (A) Extraction recoveries of ACN and MeOH solvent for MLT. (B) MLT contents of rice shoot using ACN and MeOH as extraction solvent, respectively. (C) The matrix effects of ACN extract and MeOH extract for MLT analysis. The calculation of matrix factor (MF): the peak area response for MLT- d 4 in the presence of the plant matrix before injection was compared with peak area response in the absence of matrix. Asterisk symbols ∗ P

    Article Snippet: Stable isotope-labeled standard, [2 H4 ] MLT, was purchased from C/D/N Isotopes Inc. (Pointe-Claire, QC, Canada), Chromatography grade acetonitrile (ACN) and methanol (MeOH) were obtained from TEDIA Co. (Fairfield, OH, USA).

    Techniques: Injection