mesenchymal stem cell basal medium Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Lonza mesenchymal stem cell basal medium
    Mesenchymal Stem Cell Basal Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mesenchymal stem cell basal medium/product/Lonza
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mesenchymal stem cell basal medium - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    mscs  (ATCC)
    99
    ATCC mscs
    Effect of <t>TAK1</t> on the expression levels of the osteoblastic marker genes during osteogenic differentiation of <t>MSCs.</t> qRT-PCR analysis of the osteoblastic marker genes bone sialoprotein (BSP), osteocalcin (OSC), Alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX2). GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p
    Mscs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mscs/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mscs - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of TAK1 on the expression levels of the osteoblastic marker genes during osteogenic differentiation of MSCs. qRT-PCR analysis of the osteoblastic marker genes bone sialoprotein (BSP), osteocalcin (OSC), Alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX2). GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Journal: Organogenesis

    Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway

    doi: 10.1080/15476278.2018.1455010

    Figure Lengend Snippet: Effect of TAK1 on the expression levels of the osteoblastic marker genes during osteogenic differentiation of MSCs. qRT-PCR analysis of the osteoblastic marker genes bone sialoprotein (BSP), osteocalcin (OSC), Alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX2). GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.

    Techniques: Expressing, Marker, Quantitative RT-PCR, ALP Assay, Transfection

    p38 and JNK down regulated the effect of TAK1 on the osteogenic differentiation of MSCs. (A) The mRNA level of BMP-2 with p38 inhibitor (SB203580). (B) The mRNA level of BMP-2 with JNK inhibitor (SP600125). (C) The mRNA level of BMP-2 with p38 inhibitor (SB203580) and JNK inhibitor (SP600125). Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Journal: Organogenesis

    Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway

    doi: 10.1080/15476278.2018.1455010

    Figure Lengend Snippet: p38 and JNK down regulated the effect of TAK1 on the osteogenic differentiation of MSCs. (A) The mRNA level of BMP-2 with p38 inhibitor (SB203580). (B) The mRNA level of BMP-2 with JNK inhibitor (SP600125). (C) The mRNA level of BMP-2 with p38 inhibitor (SB203580) and JNK inhibitor (SP600125). Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.

    Techniques: Transfection

    Effect of TAK1 on the expression levels of β-catenin and GSK-3b during osteogenic differentiation of MSCs. qRT-PCR (A, B) and western blot (C, D) analysis of the genes and corresponding proteins. GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, or added with 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Journal: Organogenesis

    Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway

    doi: 10.1080/15476278.2018.1455010

    Figure Lengend Snippet: Effect of TAK1 on the expression levels of β-catenin and GSK-3b during osteogenic differentiation of MSCs. qRT-PCR (A, B) and western blot (C, D) analysis of the genes and corresponding proteins. GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, or added with 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection

    Effect of TAK1 on the mineralization of MSCs. (A) The expression of TAK1 was downregulated by si-TAK1 and analyzed by western blot, with antibodies against TAK1 and GAPDH, respectively. (B) Mineralization was quantitated through the elution of Alizarin Red S from stained mineral deposits. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 10 and 20 days. Values are expressed as means ± S.E.M. of three independent experiments. **p

    Journal: Organogenesis

    Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway

    doi: 10.1080/15476278.2018.1455010

    Figure Lengend Snippet: Effect of TAK1 on the mineralization of MSCs. (A) The expression of TAK1 was downregulated by si-TAK1 and analyzed by western blot, with antibodies against TAK1 and GAPDH, respectively. (B) Mineralization was quantitated through the elution of Alizarin Red S from stained mineral deposits. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 10 and 20 days. Values are expressed as means ± S.E.M. of three independent experiments. **p

    Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.

    Techniques: Expressing, Western Blot, Staining, Transfection

    Effects of TAK1 on the expression levels of p38 and JNK during osteogenic differentiation of MSCs. (A) Western blot analysis of the protein expression levels. (B) The bar graph showed the intensities of protein expressions compared with GAPDH. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Journal: Organogenesis

    Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway

    doi: 10.1080/15476278.2018.1455010

    Figure Lengend Snippet: Effects of TAK1 on the expression levels of p38 and JNK during osteogenic differentiation of MSCs. (A) Western blot analysis of the protein expression levels. (B) The bar graph showed the intensities of protein expressions compared with GAPDH. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p

    Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.

    Techniques: Expressing, Western Blot, Transfection

    Effect of TAK1 on the expression levels of TGF-β and BMP-2 during osteogenic differentiation of MSCs. qRT-PCR (A,B) and western blot (C) analysis of the genes. GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. ** p

    Journal: Organogenesis

    Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway

    doi: 10.1080/15476278.2018.1455010

    Figure Lengend Snippet: Effect of TAK1 on the expression levels of TGF-β and BMP-2 during osteogenic differentiation of MSCs. qRT-PCR (A,B) and western blot (C) analysis of the genes. GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. ** p

    Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection