Article Title: Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway
Figure Lengend Snippet: Effect of TAK1 on the expression levels of the osteoblastic marker genes during osteogenic differentiation of MSCs. qRT-PCR analysis of the osteoblastic marker genes bone sialoprotein (BSP), osteocalcin (OSC), Alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX2). GAPDH was used as an internal control. Cells were plated at a density of 2 × 10 4 cells/cm 2 in 6-well plates. After 2 days, the medium was replaced with an osteogenic -inducing medium. Then, the cells were transfected with si-TAK1, 1 ng/ml rhTAK1 (rh1), 5 ng/ml rhTAK1 (rh5) or 50 ng/ml rhTAK1 (rh50), respectively, for 14 days. Values are expressed as means ± S.E.M. of three independent experiments. * p
Article Snippet: To knockdown TAK1 in MSCs, siRNAs targeting TAK1 were transfected in MSCs using GeneXPlus transfection reagent (ATCC ACS-4004) according to the manufacture's instruction.
Techniques: Expressing, Marker, Quantitative RT-PCR, ALP Assay, Transfection