mesangial mrna Search Results


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  • 99
    Thermo Fisher mesangial cell basal medium
    Mesangial Cell Basal Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL nucleotrap mrna kit
    Nucleotrap Mrna Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 88/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche mrna levels
    Mrna Levels, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 2450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IGAN Bio mesangial cells
    Circulating IgA1-containing immune complexes (cIgA1) activated human <t>mesangial</t> cells. Compared to those from healthy controls, cIgA1 from patients with IgA nephropathy significantly upregulated human mesangial cell proliferation ( a ; 1.15 ± 0.08 vs. 1.08 ± 0.06, p = 0.002) as well as secretion of MCP-1 ( b ; 1,470.4 ± 264.6 vs. 1,028.5 ± 166.0 pg/mL, p
    Mesangial Cells, supplied by IGAN Bio, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DS Pharma Biomedical human mesangial cells human mesangial cells
    Effect of podocan on TGF- β –induced profibrotic gene expression in human <t>mesangial</t> cells. Human mesangial cells were seeded on a type 1, collagen-coated, 24-well plate on day 0. On day 1, the cells were serum starved with CSC medium. On day 2, recombinant human podocan at final concentrations of 0, 1, 10, 100, and 1000 ng/mL with TGF- β (final concentration of 1 ng/mL) or PBS (vehicle, without TGF- β ) was added to the culture medium. The cells were lysed after 24 hours. mRNA levels of (a) podocan, (b) collagen 1A1, (c) fibronectin, (d) CTGF, (e) and PAI-1 were measured by quantitative real-time RT-PCR. Data are expressed as the mean ± SD (n = 4 per treatment group). * P
    Human Mesangial Cells Human Mesangial Cells, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IGAN Bio human mesangial cells
    Circulating IgA1-containing immune complexes (cIgA1) activated human <t>mesangial</t> cells. Compared to those from healthy controls, cIgA1 from patients with IgA nephropathy significantly upregulated human mesangial cell proliferation ( a ; 1.15 ± 0.08 vs. 1.08 ± 0.06, p = 0.002) as well as secretion of MCP-1 ( b ; 1,470.4 ± 264.6 vs. 1,028.5 ± 166.0 pg/mL, p
    Human Mesangial Cells, supplied by IGAN Bio, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega mesangial cells
    Glomerular histology (PAS × 200). A: 5 months. Glomeruli are normal. B: 22 months. The <t>mesangial</t> area is markedly expanded. C: 30 months. Glomeruli are large. Cell number is increased. The mesangial area is increased, compared to 22 months.
    Mesangial Cells, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mesangial cell
    Rat cultured <t>mesangial</t> cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,
    Mesangial Cell, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Schiff Nutrition International mesangial expansion data
    Rat cultured <t>mesangial</t> cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,
    Mesangial Expansion Data, supplied by Schiff Nutrition International, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC rat mesangial cells
    Rat cultured <t>mesangial</t> cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,
    Rat Mesangial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 89/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cambrex clonetics primary human mesangial cells
    Rat cultured <t>mesangial</t> cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,
    Clonetics Primary Human Mesangial Cells, supplied by Cambrex, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mes13 mouse mesangial cell line
    Rat cultured <t>mesangial</t> cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,
    Mes13 Mouse Mesangial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp col4a1 mm01210125 m1
    Presence and activation of ErbB receptors and effect of NRG-1 on urinary markers, glomerulosclerosis, and renal fibrotic markers. A : immunohistochemical staining of a mouse kidney for the presence of ErbB2, ErbB3, and ErbB4 tyrosine kinase receptors in renal tubular and glomerular cells. B : levels of phosphorylated ErbB2 and ErbB4 in cardiac and renal tissue of C57Bl/6 mice treated with either vehicle (−) or rhNRG-1 (+). GAPDH abundancy in equal amounts of samples used for immunoprecipitation is shown below. C : kidney function markers. Bar graphs showing urinary albumin and urinary NGAL concentrations, and creatinine clearance. D : representative images and quantification of renal glomerular fibrotic area. E : bar graphs showing mRNA expression in whole kidney tissue of TGFβ1, FSP-1, and <t>Col4a1,</t> expressed as fold induction to apoE −/− control + vehicle mice. FSP-1, fibroblast-specific protein-1; Col4a1, procollagen 4a1; TGFβ1, transforming growth factor-β1; UAE, urinary albumin excretion; NGAL, neutrophil gelatinase-associated lipocalin. * P
    Gene Exp Col4a1 Mm01210125 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp myh7 mm01319006 g1
    Presence and activation of ErbB receptors and effect of NRG-1 on urinary markers, glomerulosclerosis, and renal fibrotic markers. A : immunohistochemical staining of a mouse kidney for the presence of ErbB2, ErbB3, and ErbB4 tyrosine kinase receptors in renal tubular and glomerular cells. B : levels of phosphorylated ErbB2 and ErbB4 in cardiac and renal tissue of C57Bl/6 mice treated with either vehicle (−) or rhNRG-1 (+). GAPDH abundancy in equal amounts of samples used for immunoprecipitation is shown below. C : kidney function markers. Bar graphs showing urinary albumin and urinary NGAL concentrations, and creatinine clearance. D : representative images and quantification of renal glomerular fibrotic area. E : bar graphs showing mRNA expression in whole kidney tissue of TGFβ1, FSP-1, and <t>Col4a1,</t> expressed as fold induction to apoE −/− control + vehicle mice. FSP-1, fibroblast-specific protein-1; Col4a1, procollagen 4a1; TGFβ1, transforming growth factor-β1; UAE, urinary albumin excretion; NGAL, neutrophil gelatinase-associated lipocalin. * P
    Gene Exp Myh7 Mm01319006 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems ccl5 rantes
    Presence and activation of ErbB receptors and effect of NRG-1 on urinary markers, glomerulosclerosis, and renal fibrotic markers. A : immunohistochemical staining of a mouse kidney for the presence of ErbB2, ErbB3, and ErbB4 tyrosine kinase receptors in renal tubular and glomerular cells. B : levels of phosphorylated ErbB2 and ErbB4 in cardiac and renal tissue of C57Bl/6 mice treated with either vehicle (−) or rhNRG-1 (+). GAPDH abundancy in equal amounts of samples used for immunoprecipitation is shown below. C : kidney function markers. Bar graphs showing urinary albumin and urinary NGAL concentrations, and creatinine clearance. D : representative images and quantification of renal glomerular fibrotic area. E : bar graphs showing mRNA expression in whole kidney tissue of TGFβ1, FSP-1, and <t>Col4a1,</t> expressed as fold induction to apoE −/− control + vehicle mice. FSP-1, fibroblast-specific protein-1; Col4a1, procollagen 4a1; TGFβ1, transforming growth factor-β1; UAE, urinary albumin excretion; NGAL, neutrophil gelatinase-associated lipocalin. * P
    Ccl5 Rantes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems tnfα
    Effects of IL-17 on chemokine expression in mMCs. (A) Detection of IL-17RA and IL-17RC mRNA expression in mouse mMCs by RT-PCR. (B) mMCs were incubated with IL-17 (10 ng/ml) in the absence or presence of <t>TNFα</t> (10 ng/ml) for 4 h. The mRNA chemokine
    Tnfα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems ccl2 mcp
    Effects of IL-17 on chemokine expression in mMCs. (A) Detection of IL-17RA and IL-17RC mRNA expression in mouse mMCs by RT-PCR. (B) mMCs were incubated with IL-17 (10 ng/ml) in the absence or presence of <t>TNFα</t> (10 ng/ml) for 4 h. The mRNA chemokine
    Ccl2 Mcp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology icam 1
    Effects of IL-17 on chemokine expression in mMCs. (A) Detection of IL-17RA and IL-17RC mRNA expression in mouse mMCs by RT-PCR. (B) mMCs were incubated with IL-17 (10 ng/ml) in the absence or presence of <t>TNFα</t> (10 ng/ml) for 4 h. The mRNA chemokine
    Icam 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 2370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems ccl3 mip 1 α
    Effects of IL-17 on chemokine expression in mMCs. (A) Detection of IL-17RA and IL-17RC mRNA expression in mouse mMCs by RT-PCR. (B) mMCs were incubated with IL-17 (10 ng/ml) in the absence or presence of <t>TNFα</t> (10 ng/ml) for 4 h. The mRNA chemokine
    Ccl3 Mip 1 α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fgf2  (Abcam)
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    Abcam fgf2
    In Vitro Analysis of FGFRs, <t>FGF2,</t> and GPC5 Expression
    Fgf2, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad ptc 200 peltier thermal cycler
    In Vitro Analysis of FGFRs, <t>FGF2,</t> and GPC5 Expression
    Ptc 200 Peltier Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher viia 7 real time pcr system
    In Vitro Analysis of FGFRs, <t>FGF2,</t> and GPC5 Expression
    Viia 7 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher custom taqman array 384 well cards
    In Vitro Analysis of FGFRs, <t>FGF2,</t> and GPC5 Expression
    Custom Taqman Array 384 Well Cards, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cationic lipid lipofectamine 2000
    Viral dsRNA complexed with cationic lipids activates mesangial cells to produce high amounts of Il-6 and type I IFN via a Trif-independent pathway. Primary mesangial cells were isolated from wild-type C56BL/6 mice and Trif -mutant mice as described in Materials and Methods . Cells were stimulated with increasing doses of poly I:C (pI:C) RNA alone or complexed with the cationic lipid (CL) <t>Lipofectamine.</t> Supernatants were harvested after 24 hours and analyzed by ELISA for the following mediators: Il-6 ( A ), IFN-α ( B ), and IFN-β ( C ). Data are means ± SEM from three experiments each analyzed in duplicate and presented in a logarithmic scale. ∗ P
    Cationic Lipid Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    InvivoGen lipofectamine
    Viral dsRNA complexed with cationic lipids activates mesangial cells to produce high amounts of Il-6 and type I IFN via a Trif-independent pathway. Primary mesangial cells were isolated from wild-type C56BL/6 mice and Trif -mutant mice as described in Materials and Methods . Cells were stimulated with increasing doses of poly I:C (pI:C) RNA alone or complexed with the cationic lipid (CL) <t>Lipofectamine.</t> Supernatants were harvested after 24 hours and analyzed by ELISA for the following mediators: Il-6 ( A ), IFN-α ( B ), and IFN-β ( C ). Data are means ± SEM from three experiments each analyzed in duplicate and presented in a logarithmic scale. ∗ P
    Lipofectamine, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Circulating IgA1-containing immune complexes (cIgA1) activated human mesangial cells. Compared to those from healthy controls, cIgA1 from patients with IgA nephropathy significantly upregulated human mesangial cell proliferation ( a ; 1.15 ± 0.08 vs. 1.08 ± 0.06, p = 0.002) as well as secretion of MCP-1 ( b ; 1,470.4 ± 264.6 vs. 1,028.5 ± 166.0 pg/mL, p

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: Circulating IgA1-containing immune complexes (cIgA1) activated human mesangial cells. Compared to those from healthy controls, cIgA1 from patients with IgA nephropathy significantly upregulated human mesangial cell proliferation ( a ; 1.15 ± 0.08 vs. 1.08 ± 0.06, p = 0.002) as well as secretion of MCP-1 ( b ; 1,470.4 ± 264.6 vs. 1,028.5 ± 166.0 pg/mL, p

    Article Snippet: Positive correlations were observed between mesangial cell proliferation and the mRNA expression of TREM-1 (correlation coefficient = 0.315, p = 0.023; Fig. ) as well as ΔsTREM-1 levels (correlation coefficient = 0.347, p = 0.012; Fig. ) in the supernatant of cultured mesangial cells.

    Techniques:

    Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated TREM-1 expression in mesangial cells. a The mRNA expression of TREM-1 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (2.39 [1.35, 4.24] vs. 1.50 [1.18, 1.85], p = 0.049). b Additionally, the levels of ΔsTREM-1 in the supernatant of mesangial cells were also significantly higher after treatment with cIgA1 derived from IgAN patients, compared to those from healthy controls (18.7 [–36.1, 97.1] vs. −34.5 [–95.8, 9.1] pg/mL, p = 0.003).

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated TREM-1 expression in mesangial cells. a The mRNA expression of TREM-1 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (2.39 [1.35, 4.24] vs. 1.50 [1.18, 1.85], p = 0.049). b Additionally, the levels of ΔsTREM-1 in the supernatant of mesangial cells were also significantly higher after treatment with cIgA1 derived from IgAN patients, compared to those from healthy controls (18.7 [–36.1, 97.1] vs. −34.5 [–95.8, 9.1] pg/mL, p = 0.003).

    Article Snippet: Positive correlations were observed between mesangial cell proliferation and the mRNA expression of TREM-1 (correlation coefficient = 0.315, p = 0.023; Fig. ) as well as ΔsTREM-1 levels (correlation coefficient = 0.347, p = 0.012; Fig. ) in the supernatant of cultured mesangial cells.

    Techniques: Expressing, Derivative Assay

    Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated MMP9 expression in mesangial cells. a The mRNA expression of MMP9 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (1.01 [0.76, 1.99] vs. 0.57 [0.41, 0.97], p = 0.003). b Moreover, the expression of MMP9 and TREM-1 showed positive correlation in mesangial cells after cIgA1 treatment ( r = 0.551, p

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated MMP9 expression in mesangial cells. a The mRNA expression of MMP9 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (1.01 [0.76, 1.99] vs. 0.57 [0.41, 0.97], p = 0.003). b Moreover, the expression of MMP9 and TREM-1 showed positive correlation in mesangial cells after cIgA1 treatment ( r = 0.551, p

    Article Snippet: Positive correlations were observed between mesangial cell proliferation and the mRNA expression of TREM-1 (correlation coefficient = 0.315, p = 0.023; Fig. ) as well as ΔsTREM-1 levels (correlation coefficient = 0.347, p = 0.012; Fig. ) in the supernatant of cultured mesangial cells.

    Techniques: Expressing, Derivative Assay

    The expression of TREM-1 correlated with activation of mesangial cells. Mesangial cell proliferation showed a pos­itive correlation with both mRNA ex pression of TREM-1 ( a ; correlation coefficient = 0.315, p = 0.023) and ΔsTREM-1 levels in the supernatant of cultured mesangial cells ( b ; correlation coefficient = 0.347, p = 0.012).

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: The expression of TREM-1 correlated with activation of mesangial cells. Mesangial cell proliferation showed a pos­itive correlation with both mRNA ex pression of TREM-1 ( a ; correlation coefficient = 0.315, p = 0.023) and ΔsTREM-1 levels in the supernatant of cultured mesangial cells ( b ; correlation coefficient = 0.347, p = 0.012).

    Article Snippet: Positive correlations were observed between mesangial cell proliferation and the mRNA expression of TREM-1 (correlation coefficient = 0.315, p = 0.023; Fig. ) as well as ΔsTREM-1 levels (correlation coefficient = 0.347, p = 0.012; Fig. ) in the supernatant of cultured mesangial cells.

    Techniques: Expressing, Activation Assay, Cell Culture

    Effect of podocan on TGF- β –induced profibrotic gene expression in human mesangial cells. Human mesangial cells were seeded on a type 1, collagen-coated, 24-well plate on day 0. On day 1, the cells were serum starved with CSC medium. On day 2, recombinant human podocan at final concentrations of 0, 1, 10, 100, and 1000 ng/mL with TGF- β (final concentration of 1 ng/mL) or PBS (vehicle, without TGF- β ) was added to the culture medium. The cells were lysed after 24 hours. mRNA levels of (a) podocan, (b) collagen 1A1, (c) fibronectin, (d) CTGF, (e) and PAI-1 were measured by quantitative real-time RT-PCR. Data are expressed as the mean ± SD (n = 4 per treatment group). * P

    Journal: Journal of the Endocrine Society

    Article Title: Podocan Is Expressed in Blood and Adipose Tissue and Correlates Negatively With the Induction of Diabetic Nephropathy

    doi: 10.1210/js.2017-00123

    Figure Lengend Snippet: Effect of podocan on TGF- β –induced profibrotic gene expression in human mesangial cells. Human mesangial cells were seeded on a type 1, collagen-coated, 24-well plate on day 0. On day 1, the cells were serum starved with CSC medium. On day 2, recombinant human podocan at final concentrations of 0, 1, 10, 100, and 1000 ng/mL with TGF- β (final concentration of 1 ng/mL) or PBS (vehicle, without TGF- β ) was added to the culture medium. The cells were lysed after 24 hours. mRNA levels of (a) podocan, (b) collagen 1A1, (c) fibronectin, (d) CTGF, (e) and PAI-1 were measured by quantitative real-time RT-PCR. Data are expressed as the mean ± SD (n = 4 per treatment group). * P

    Article Snippet: L. mRNA Expression Analysis by Quantitative Real-Time PCR in Human Mesangial Cells Human mesangial cells were purchased from DS Pharma Biomedical (Osaka, Japan).

    Techniques: Expressing, Recombinant, Concentration Assay, Quantitative RT-PCR

    Circulating IgA1-containing immune complexes (cIgA1) activated human mesangial cells. Compared to those from healthy controls, cIgA1 from patients with IgA nephropathy significantly upregulated human mesangial cell proliferation ( a ; 1.15 ± 0.08 vs. 1.08 ± 0.06, p = 0.002) as well as secretion of MCP-1 ( b ; 1,470.4 ± 264.6 vs. 1,028.5 ± 166.0 pg/mL, p

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: Circulating IgA1-containing immune complexes (cIgA1) activated human mesangial cells. Compared to those from healthy controls, cIgA1 from patients with IgA nephropathy significantly upregulated human mesangial cell proliferation ( a ; 1.15 ± 0.08 vs. 1.08 ± 0.06, p = 0.002) as well as secretion of MCP-1 ( b ; 1,470.4 ± 264.6 vs. 1,028.5 ± 166.0 pg/mL, p

    Article Snippet: Here, we detected the expression of 2 kinds of matrix metalloproteinases, MMP9 and MMP2, in human mesangial cells.

    Techniques:

    Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated TREM-1 expression in mesangial cells. a The mRNA expression of TREM-1 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (2.39 [1.35, 4.24] vs. 1.50 [1.18, 1.85], p = 0.049). b Additionally, the levels of ΔsTREM-1 in the supernatant of mesangial cells were also significantly higher after treatment with cIgA1 derived from IgAN patients, compared to those from healthy controls (18.7 [–36.1, 97.1] vs. −34.5 [–95.8, 9.1] pg/mL, p = 0.003).

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated TREM-1 expression in mesangial cells. a The mRNA expression of TREM-1 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (2.39 [1.35, 4.24] vs. 1.50 [1.18, 1.85], p = 0.049). b Additionally, the levels of ΔsTREM-1 in the supernatant of mesangial cells were also significantly higher after treatment with cIgA1 derived from IgAN patients, compared to those from healthy controls (18.7 [–36.1, 97.1] vs. −34.5 [–95.8, 9.1] pg/mL, p = 0.003).

    Article Snippet: Here, we detected the expression of 2 kinds of matrix metalloproteinases, MMP9 and MMP2, in human mesangial cells.

    Techniques: Expressing, Derivative Assay

    Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated MMP9 expression in mesangial cells. a The mRNA expression of MMP9 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (1.01 [0.76, 1.99] vs. 0.57 [0.41, 0.97], p = 0.003). b Moreover, the expression of MMP9 and TREM-1 showed positive correlation in mesangial cells after cIgA1 treatment ( r = 0.551, p

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: Circulating IgA1-containing immune complexes (cIgA1) from IgA nephropathy (IgAN) patients induced upregulated MMP9 expression in mesangial cells. a The mRNA expression of MMP9 in human mesangial cells was significantly upregulated by cIgA1 derived from IgAN patients (1.01 [0.76, 1.99] vs. 0.57 [0.41, 0.97], p = 0.003). b Moreover, the expression of MMP9 and TREM-1 showed positive correlation in mesangial cells after cIgA1 treatment ( r = 0.551, p

    Article Snippet: Here, we detected the expression of 2 kinds of matrix metalloproteinases, MMP9 and MMP2, in human mesangial cells.

    Techniques: Expressing, Derivative Assay

    The expression of TREM-1 correlated with activation of mesangial cells. Mesangial cell proliferation showed a pos­itive correlation with both mRNA ex pression of TREM-1 ( a ; correlation coefficient = 0.315, p = 0.023) and ΔsTREM-1 levels in the supernatant of cultured mesangial cells ( b ; correlation coefficient = 0.347, p = 0.012).

    Journal: Kidney Diseases

    Article Title: TREM-1 Contributes to Inflammation in IgA Nephropathy

    doi: 10.1159/000485622

    Figure Lengend Snippet: The expression of TREM-1 correlated with activation of mesangial cells. Mesangial cell proliferation showed a pos­itive correlation with both mRNA ex pression of TREM-1 ( a ; correlation coefficient = 0.315, p = 0.023) and ΔsTREM-1 levels in the supernatant of cultured mesangial cells ( b ; correlation coefficient = 0.347, p = 0.012).

    Article Snippet: Here, we detected the expression of 2 kinds of matrix metalloproteinases, MMP9 and MMP2, in human mesangial cells.

    Techniques: Expressing, Activation Assay, Cell Culture

    Glomerular histology (PAS × 200). A: 5 months. Glomeruli are normal. B: 22 months. The mesangial area is markedly expanded. C: 30 months. Glomeruli are large. Cell number is increased. The mesangial area is increased, compared to 22 months.

    Journal: The American Journal of Pathology

    Article Title: Resistance to Glomerulosclerosis in B6 Mice Disappears after Menopause

    doi:

    Figure Lengend Snippet: Glomerular histology (PAS × 200). A: 5 months. Glomeruli are normal. B: 22 months. The mesangial area is markedly expanded. C: 30 months. Glomeruli are large. Cell number is increased. The mesangial area is increased, compared to 22 months.

    Article Snippet: Similarly, α1 type I collagen mRNA and protein levels were higher in cells isolated from 28-month-old mouse (Figure 11, B–D) . α1 type IV collagen mRNA levels were also increased 1.5-fold in 28-month-old compared to 5-month-old mesangial cells (Figure 11E) .

    Techniques:

    Glomerular histology of uninephrectomized mice. (PAS × 200). A: 2.5 months. There is increase in glomerular volume but not mesangial area. B: 20 months. There is significant increase in glomerular mesangial area.

    Journal: The American Journal of Pathology

    Article Title: Resistance to Glomerulosclerosis in B6 Mice Disappears after Menopause

    doi:

    Figure Lengend Snippet: Glomerular histology of uninephrectomized mice. (PAS × 200). A: 2.5 months. There is increase in glomerular volume but not mesangial area. B: 20 months. There is significant increase in glomerular mesangial area.

    Article Snippet: Similarly, α1 type I collagen mRNA and protein levels were higher in cells isolated from 28-month-old mouse (Figure 11, B–D) . α1 type IV collagen mRNA levels were also increased 1.5-fold in 28-month-old compared to 5-month-old mesangial cells (Figure 11E) .

    Techniques: Mouse Assay

    TGF-β transcriptional activities and mRNA expression in mesangial cells. A: Increased TGF-β transcriptional activities in mesangial cells isolated from 28-month-old mouse (28-month MC). Basal and TGF-β1-mediated activation of TGF-β responses were assessed in mesangial cells transfected with 250 ng of a TGF-β reporter construct and 250 ng of a Rous sarcoma virus β-galactosidase plasmid in the presence or absence of 2 ng/ml TGF-β1. (**, P

    Journal: The American Journal of Pathology

    Article Title: Resistance to Glomerulosclerosis in B6 Mice Disappears after Menopause

    doi:

    Figure Lengend Snippet: TGF-β transcriptional activities and mRNA expression in mesangial cells. A: Increased TGF-β transcriptional activities in mesangial cells isolated from 28-month-old mouse (28-month MC). Basal and TGF-β1-mediated activation of TGF-β responses were assessed in mesangial cells transfected with 250 ng of a TGF-β reporter construct and 250 ng of a Rous sarcoma virus β-galactosidase plasmid in the presence or absence of 2 ng/ml TGF-β1. (**, P

    Article Snippet: Similarly, α1 type I collagen mRNA and protein levels were higher in cells isolated from 28-month-old mouse (Figure 11, B–D) . α1 type IV collagen mRNA levels were also increased 1.5-fold in 28-month-old compared to 5-month-old mesangial cells (Figure 11E) .

    Techniques: Expressing, Isolation, Activation Assay, Transfection, Construct, Plasmid Preparation

    Type I collagen transcriptional activities and type I and type IV collagen expression in mesangial cells. A: Increased type I collagen transcriptional activity in mesangial cells isolated from 28-month-old mouse (28-month MC). Basal and TGF-β1-mediated activation of type I collagen transcription was assessed in mesangial cells transfected with 250 ng of a type I collagen promoter reporter construct and 250 ng of a Rous sarcoma virus β-galactosidase plasmid in the presence or absence of 2 ng/ml TGF-β1. (**, P

    Journal: The American Journal of Pathology

    Article Title: Resistance to Glomerulosclerosis in B6 Mice Disappears after Menopause

    doi:

    Figure Lengend Snippet: Type I collagen transcriptional activities and type I and type IV collagen expression in mesangial cells. A: Increased type I collagen transcriptional activity in mesangial cells isolated from 28-month-old mouse (28-month MC). Basal and TGF-β1-mediated activation of type I collagen transcription was assessed in mesangial cells transfected with 250 ng of a type I collagen promoter reporter construct and 250 ng of a Rous sarcoma virus β-galactosidase plasmid in the presence or absence of 2 ng/ml TGF-β1. (**, P

    Article Snippet: Similarly, α1 type I collagen mRNA and protein levels were higher in cells isolated from 28-month-old mouse (Figure 11, B–D) . α1 type IV collagen mRNA levels were also increased 1.5-fold in 28-month-old compared to 5-month-old mesangial cells (Figure 11E) .

    Techniques: Expressing, Activity Assay, Isolation, Activation Assay, Transfection, Construct, Plasmid Preparation

    Progressive increase in glomerular mesangial area with age. (**, P

    Journal: The American Journal of Pathology

    Article Title: Resistance to Glomerulosclerosis in B6 Mice Disappears after Menopause

    doi:

    Figure Lengend Snippet: Progressive increase in glomerular mesangial area with age. (**, P

    Article Snippet: Similarly, α1 type I collagen mRNA and protein levels were higher in cells isolated from 28-month-old mouse (Figure 11, B–D) . α1 type IV collagen mRNA levels were also increased 1.5-fold in 28-month-old compared to 5-month-old mesangial cells (Figure 11E) .

    Techniques:

    Rat cultured mesangial cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,

    Journal:

    Article Title: Hemoglobin Is Expressed by Mesangial Cells and Reduces Oxidant Stress

    doi: 10.1681/ASN.2007101085

    Figure Lengend Snippet: Rat cultured mesangial cells express globin subunit mRNA. Expression of globins in several cultured rat glomerular cells, such as primary cultured rat mesangial cells, podocytes, and endothelial cells, was confirmed by RT-PCR. MES, mesangial cells; POD,

    Article Snippet: To construct an optimal vector expressing both α- and β-globin subunits in mesangial cell, we used pIRES (Clontech Palo Alto, CA) vector that allows expression of two genes of interest by cloning them into multiple cloning sites (MCS) A and B.

    Techniques: Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction

    In situ hybridization studies showed globin subunit mRNA expression in the mesangial region. (A) Hybridization using antisense probe evidenced expression of α- and β-globin genes in rat glomeruli. Of note, the signals were localized in

    Journal:

    Article Title: Hemoglobin Is Expressed by Mesangial Cells and Reduces Oxidant Stress

    doi: 10.1681/ASN.2007101085

    Figure Lengend Snippet: In situ hybridization studies showed globin subunit mRNA expression in the mesangial region. (A) Hybridization using antisense probe evidenced expression of α- and β-globin genes in rat glomeruli. Of note, the signals were localized in

    Article Snippet: To construct an optimal vector expressing both α- and β-globin subunits in mesangial cell, we used pIRES (Clontech Palo Alto, CA) vector that allows expression of two genes of interest by cloning them into multiple cloning sites (MCS) A and B.

    Techniques: In Situ Hybridization, Expressing, Hybridization

    Double-immunofluorescence studies of rat kidney showed localization of Hb protein in mesangial region. (A) Immunofluorescence of the normal rat kidney with antibody to Hb was observed in the glomerular mesangial region in green. (B) Higher magnification

    Journal:

    Article Title: Hemoglobin Is Expressed by Mesangial Cells and Reduces Oxidant Stress

    doi: 10.1681/ASN.2007101085

    Figure Lengend Snippet: Double-immunofluorescence studies of rat kidney showed localization of Hb protein in mesangial region. (A) Immunofluorescence of the normal rat kidney with antibody to Hb was observed in the glomerular mesangial region in green. (B) Higher magnification

    Article Snippet: To construct an optimal vector expressing both α- and β-globin subunits in mesangial cell, we used pIRES (Clontech Palo Alto, CA) vector that allows expression of two genes of interest by cloning them into multiple cloning sites (MCS) A and B.

    Techniques: Immunofluorescence

    Transfection of murine mesangial cells with the globin-expressing vector suppressed intracellular ROS generation. (A) The construct pIRES/α-globin/β-globin was designed to express effectively both α- and β-globin subunits

    Journal:

    Article Title: Hemoglobin Is Expressed by Mesangial Cells and Reduces Oxidant Stress

    doi: 10.1681/ASN.2007101085

    Figure Lengend Snippet: Transfection of murine mesangial cells with the globin-expressing vector suppressed intracellular ROS generation. (A) The construct pIRES/α-globin/β-globin was designed to express effectively both α- and β-globin subunits

    Article Snippet: To construct an optimal vector expressing both α- and β-globin subunits in mesangial cell, we used pIRES (Clontech Palo Alto, CA) vector that allows expression of two genes of interest by cloning them into multiple cloning sites (MCS) A and B.

    Techniques: Transfection, Expressing, Plasmid Preparation, Construct

    Presence and activation of ErbB receptors and effect of NRG-1 on urinary markers, glomerulosclerosis, and renal fibrotic markers. A : immunohistochemical staining of a mouse kidney for the presence of ErbB2, ErbB3, and ErbB4 tyrosine kinase receptors in renal tubular and glomerular cells. B : levels of phosphorylated ErbB2 and ErbB4 in cardiac and renal tissue of C57Bl/6 mice treated with either vehicle (−) or rhNRG-1 (+). GAPDH abundancy in equal amounts of samples used for immunoprecipitation is shown below. C : kidney function markers. Bar graphs showing urinary albumin and urinary NGAL concentrations, and creatinine clearance. D : representative images and quantification of renal glomerular fibrotic area. E : bar graphs showing mRNA expression in whole kidney tissue of TGFβ1, FSP-1, and Col4a1, expressed as fold induction to apoE −/− control + vehicle mice. FSP-1, fibroblast-specific protein-1; Col4a1, procollagen 4a1; TGFβ1, transforming growth factor-β1; UAE, urinary albumin excretion; NGAL, neutrophil gelatinase-associated lipocalin. * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Neuregulin-1 attenuates development of nephropathy in a type 1 diabetes mouse model with high cardiovascular risk

    doi: 10.1152/ajpendo.00432.2015

    Figure Lengend Snippet: Presence and activation of ErbB receptors and effect of NRG-1 on urinary markers, glomerulosclerosis, and renal fibrotic markers. A : immunohistochemical staining of a mouse kidney for the presence of ErbB2, ErbB3, and ErbB4 tyrosine kinase receptors in renal tubular and glomerular cells. B : levels of phosphorylated ErbB2 and ErbB4 in cardiac and renal tissue of C57Bl/6 mice treated with either vehicle (−) or rhNRG-1 (+). GAPDH abundancy in equal amounts of samples used for immunoprecipitation is shown below. C : kidney function markers. Bar graphs showing urinary albumin and urinary NGAL concentrations, and creatinine clearance. D : representative images and quantification of renal glomerular fibrotic area. E : bar graphs showing mRNA expression in whole kidney tissue of TGFβ1, FSP-1, and Col4a1, expressed as fold induction to apoE −/− control + vehicle mice. FSP-1, fibroblast-specific protein-1; Col4a1, procollagen 4a1; TGFβ1, transforming growth factor-β1; UAE, urinary albumin excretion; NGAL, neutrophil gelatinase-associated lipocalin. * P

    Article Snippet: TGF-β1, procollagen 4a1 (Mm01210125_m1, Col4a1) and fibroblast-specific protein-1 (FSP-1, Mm01210125_m1, s100a4) mRNA expression was analyzed in whole kidney tissue.

    Techniques: Activation Assay, Immunohistochemistry, Staining, Mouse Assay, Immunoprecipitation, Expressing

    Direct effect of NRG-1 on glomerular arterioles and renal glomerular mesangial cells. A : graphs showing the effect of AngII on vasoconstriction in glomerular arterioles in the absence and the presence of rhNRG-1. The selective angiotensin type 1A receptor antagonist ZD7155 served as positive control for inhibition of AngII-induced vasoconstriction. B : digital microscopic images of a mouse efferent arteriole. The free end of the arteriole is aspirated into a pipette, visible on the left . A : basal situation of efferent arteriole. B : application of rhNRG-1 (50 ng/ml) + AngII (10 −12 to 10 −6 mol/l). C : Western blot analysis confirming the presence of ErbB2 and ErbB4 receptors in mesangial cells. D : bar graphs showing TGFβ1-induced mRNA expression of Fn-1 and Col4a1 in the absence and the presence of rhNRG-1 as fold induction to control. AngII, angiotensin II; Fn-1, fibronectin-1. * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Neuregulin-1 attenuates development of nephropathy in a type 1 diabetes mouse model with high cardiovascular risk

    doi: 10.1152/ajpendo.00432.2015

    Figure Lengend Snippet: Direct effect of NRG-1 on glomerular arterioles and renal glomerular mesangial cells. A : graphs showing the effect of AngII on vasoconstriction in glomerular arterioles in the absence and the presence of rhNRG-1. The selective angiotensin type 1A receptor antagonist ZD7155 served as positive control for inhibition of AngII-induced vasoconstriction. B : digital microscopic images of a mouse efferent arteriole. The free end of the arteriole is aspirated into a pipette, visible on the left . A : basal situation of efferent arteriole. B : application of rhNRG-1 (50 ng/ml) + AngII (10 −12 to 10 −6 mol/l). C : Western blot analysis confirming the presence of ErbB2 and ErbB4 receptors in mesangial cells. D : bar graphs showing TGFβ1-induced mRNA expression of Fn-1 and Col4a1 in the absence and the presence of rhNRG-1 as fold induction to control. AngII, angiotensin II; Fn-1, fibronectin-1. * P

    Article Snippet: TGF-β1, procollagen 4a1 (Mm01210125_m1, Col4a1) and fibroblast-specific protein-1 (FSP-1, Mm01210125_m1, s100a4) mRNA expression was analyzed in whole kidney tissue.

    Techniques: Positive Control, Inhibition, Transferring, Western Blot, Expressing

    Effects of IL-17 on chemokine expression in mMCs. (A) Detection of IL-17RA and IL-17RC mRNA expression in mouse mMCs by RT-PCR. (B) mMCs were incubated with IL-17 (10 ng/ml) in the absence or presence of TNFα (10 ng/ml) for 4 h. The mRNA chemokine

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: The IL-23/Th17 Axis Contributes to Renal Injury in Experimental Glomerulonephritis

    doi: 10.1681/ASN.2008050556

    Figure Lengend Snippet: Effects of IL-17 on chemokine expression in mMCs. (A) Detection of IL-17RA and IL-17RC mRNA expression in mouse mMCs by RT-PCR. (B) mMCs were incubated with IL-17 (10 ng/ml) in the absence or presence of TNFα (10 ng/ml) for 4 h. The mRNA chemokine

    Article Snippet: Before stimulation, confluent cells were incubated in serum-free DMEM for 24 h. mMCs were stimulated with IL-17 and TNFα (RD Systems, Wiesbaden Germany).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation

    In Vitro Analysis of FGFRs, FGF2, and GPC5 Expression

    Journal: The American Journal of Pathology

    Article Title: Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney

    doi: 10.1016/j.ajpath.2015.03.025

    Figure Lengend Snippet: In Vitro Analysis of FGFRs, FGF2, and GPC5 Expression

    Article Snippet: After transfection, cells were subjected to different concentrations of glucose (300 or 600 mg/dL) for 48 hours with subsequent incubation by using different concentrations of FGF2 for 24 hours.

    Techniques: In Vitro, Expressing

    Pathogenetic role of Gpc5 and fibroblast growth factor 2 in developing diabetic albuminuria in mice. Albuminuria (milligrams of urinary albumin to grams of creatinine) was increased significantly in Akita mice after FGF2 injection; days 5, 10, and 15,

    Journal: The American Journal of Pathology

    Article Title: Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney

    doi: 10.1016/j.ajpath.2015.03.025

    Figure Lengend Snippet: Pathogenetic role of Gpc5 and fibroblast growth factor 2 in developing diabetic albuminuria in mice. Albuminuria (milligrams of urinary albumin to grams of creatinine) was increased significantly in Akita mice after FGF2 injection; days 5, 10, and 15,

    Article Snippet: After transfection, cells were subjected to different concentrations of glucose (300 or 600 mg/dL) for 48 hours with subsequent incubation by using different concentrations of FGF2 for 24 hours.

    Techniques: Mouse Assay, Injection

    Accumulation of extraglomerular fibroblast growth factor (FGF)2 in diabetic glomerulus and efficacy of Gpc5 deletion. A: FGF2 protein levels in whole lysates of the kidney of C57BL/6 and Akita mice are shown ( left ). Immunofluorescence staining of kidney

    Journal: The American Journal of Pathology

    Article Title: Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney

    doi: 10.1016/j.ajpath.2015.03.025

    Figure Lengend Snippet: Accumulation of extraglomerular fibroblast growth factor (FGF)2 in diabetic glomerulus and efficacy of Gpc5 deletion. A: FGF2 protein levels in whole lysates of the kidney of C57BL/6 and Akita mice are shown ( left ). Immunofluorescence staining of kidney

    Article Snippet: After transfection, cells were subjected to different concentrations of glucose (300 or 600 mg/dL) for 48 hours with subsequent incubation by using different concentrations of FGF2 for 24 hours.

    Techniques: Mouse Assay, Immunofluorescence, Staining

    Viral dsRNA complexed with cationic lipids activates mesangial cells to produce high amounts of Il-6 and type I IFN via a Trif-independent pathway. Primary mesangial cells were isolated from wild-type C56BL/6 mice and Trif -mutant mice as described in Materials and Methods . Cells were stimulated with increasing doses of poly I:C (pI:C) RNA alone or complexed with the cationic lipid (CL) Lipofectamine. Supernatants were harvested after 24 hours and analyzed by ELISA for the following mediators: Il-6 ( A ), IFN-α ( B ), and IFN-β ( C ). Data are means ± SEM from three experiments each analyzed in duplicate and presented in a logarithmic scale. ∗ P

    Journal: The American Journal of Pathology

    Article Title: Viral RNA Induces Type I Interferon-Dependent Cytokine Release and Cell Death in Mesangial Cells via Melanoma-Differentiation-Associated Gene-5

    doi: 10.2353/ajpath.2009.080585

    Figure Lengend Snippet: Viral dsRNA complexed with cationic lipids activates mesangial cells to produce high amounts of Il-6 and type I IFN via a Trif-independent pathway. Primary mesangial cells were isolated from wild-type C56BL/6 mice and Trif -mutant mice as described in Materials and Methods . Cells were stimulated with increasing doses of poly I:C (pI:C) RNA alone or complexed with the cationic lipid (CL) Lipofectamine. Supernatants were harvested after 24 hours and analyzed by ELISA for the following mediators: Il-6 ( A ), IFN-α ( B ), and IFN-β ( C ). Data are means ± SEM from three experiments each analyzed in duplicate and presented in a logarithmic scale. ∗ P

    Article Snippet: Primary mesangial cells were stimulated with endotoxin-free poly I:C RNA (InvivoGen, Toulouse, France), poly I:C RNA transfected with the cationic lipid Lipofectamine 2000 (Invitrogen, Carlsbad, CA) or ultrapure LPS (InvivoGen) for 24 hours in RPMI 1640 containing 5% FCS in the presence or absence of murine IFN-α (AbD Serotec, Oxford, UK), IFN-β (PBL, Piscataway, NJ), or IFN-γ (PeproTech, Rocky Hill, NJ).

    Techniques: Isolation, Mouse Assay, Mutagenesis, Enzyme-linked Immunosorbent Assay

    Viral dsRNA complexed with cationic lipids activates mesangial cells to produce high amounts of Il-6 and type I IFN via a Trif-independent pathway. Primary mesangial cells were isolated from wild-type C56BL/6 mice and Trif -mutant mice as described in Materials and Methods . Cells were stimulated with increasing doses of poly I:C (pI:C) RNA alone or complexed with the cationic lipid (CL) Lipofectamine. Supernatants were harvested after 24 hours and analyzed by ELISA for the following mediators: Il-6 ( A ), IFN-α ( B ), and IFN-β ( C ). Data are means ± SEM from three experiments each analyzed in duplicate and presented in a logarithmic scale. ∗ P

    Journal: The American Journal of Pathology

    Article Title: Viral RNA Induces Type I Interferon-Dependent Cytokine Release and Cell Death in Mesangial Cells via Melanoma-Differentiation-Associated Gene-5

    doi: 10.2353/ajpath.2009.080585

    Figure Lengend Snippet: Viral dsRNA complexed with cationic lipids activates mesangial cells to produce high amounts of Il-6 and type I IFN via a Trif-independent pathway. Primary mesangial cells were isolated from wild-type C56BL/6 mice and Trif -mutant mice as described in Materials and Methods . Cells were stimulated with increasing doses of poly I:C (pI:C) RNA alone or complexed with the cationic lipid (CL) Lipofectamine. Supernatants were harvested after 24 hours and analyzed by ELISA for the following mediators: Il-6 ( A ), IFN-α ( B ), and IFN-β ( C ). Data are means ± SEM from three experiments each analyzed in duplicate and presented in a logarithmic scale. ∗ P

    Article Snippet: Poly I:C RNA and Lipofectamine were preincubated with polymyxin B (Invivogen) before use to block any residual LPS contamination.

    Techniques: Isolation, Mouse Assay, Mutagenesis, Enzyme-linked Immunosorbent Assay