mers cov spike antibody rabbit pab Search Results


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  • 94
    Sino Biological rabbit polyclonal anti mers cov spike protein antibody
    Neutralizing activity of mAbs specific for S1 and the RBD of the S protein. Neutralization potency of RBD and S1-specific mAbs evaluated using a <t>MERS-CoV</t> EMC pseudovirus-neutralization assay (A) and a live-virus PRNT assay (B). The percent inhibition by the mAbs was determined by comparison with untreated cells. Each experiment was performed in triplicate, and data represent the mean of each experiment with standard errors.
    Rabbit Polyclonal Anti Mers Cov Spike Protein Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti mers cov spike protein antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    92
    Sino Biological mers cov s rabbit polyclonal antibodies
    Ability of mAbs to inhibit <t>MERS-CoV</t> S protein binding to DPP4-expressing cells. Inhibition of MERS-CoV S binding to DPP4 by D12 mAb was measured by image cytometry (A) and fluorescence intensity was analyzed by FlowJo histograms (B). As a control, a non-relevant isotype monoclonal Ab was used. Dose response curves of Log mAb concentration versus percent inhibition were generated for D12 (C), G2 (D), and G4 (E) mAbs. (C-E) Each dot represents duplicates. Error bars represent SEM.
    Mers Cov S Rabbit Polyclonal Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov s rabbit polyclonal antibodies/product/Sino Biological
    Average 92 stars, based on 1 article reviews
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    mers cov s rabbit polyclonal antibodies - by Bioz Stars, 2021-09
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    93
    Sino Biological mers cov spike protein s2 antibody rabbit pab
    Ability of mAbs to inhibit <t>MERS-CoV</t> S protein binding to DPP4-expressing cells. Inhibition of MERS-CoV S binding to DPP4 by D12 mAb was measured by image cytometry (A) and fluorescence intensity was analyzed by FlowJo histograms (B). As a control, a non-relevant isotype monoclonal Ab was used. Dose response curves of Log mAb concentration versus percent inhibition were generated for D12 (C), G2 (D), and G4 (E) mAbs. (C-E) Each dot represents duplicates. Error bars represent SEM.
    Mers Cov Spike Protein S2 Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov spike protein s2 antibody rabbit pab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov spike protein s2 antibody rabbit pab - by Bioz Stars, 2021-09
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    93
    Lifespan Biosciences polyclonal antibody
    Ability of mAbs to inhibit <t>MERS-CoV</t> S protein binding to DPP4-expressing cells. Inhibition of MERS-CoV S binding to DPP4 by D12 mAb was measured by image cytometry (A) and fluorescence intensity was analyzed by FlowJo histograms (B). As a control, a non-relevant isotype monoclonal Ab was used. Dose response curves of Log mAb concentration versus percent inhibition were generated for D12 (C), G2 (D), and G4 (E) mAbs. (C-E) Each dot represents duplicates. Error bars represent SEM.
    Polyclonal Antibody, supplied by Lifespan Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Lifespan Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2021-09
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    Image Search Results


    Neutralizing activity of mAbs specific for S1 and the RBD of the S protein. Neutralization potency of RBD and S1-specific mAbs evaluated using a MERS-CoV EMC pseudovirus-neutralization assay (A) and a live-virus PRNT assay (B). The percent inhibition by the mAbs was determined by comparison with untreated cells. Each experiment was performed in triplicate, and data represent the mean of each experiment with standard errors.

    Journal: PLoS ONE

    Article Title: Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus

    doi: 10.1371/journal.pone.0232757

    Figure Lengend Snippet: Neutralizing activity of mAbs specific for S1 and the RBD of the S protein. Neutralization potency of RBD and S1-specific mAbs evaluated using a MERS-CoV EMC pseudovirus-neutralization assay (A) and a live-virus PRNT assay (B). The percent inhibition by the mAbs was determined by comparison with untreated cells. Each experiment was performed in triplicate, and data represent the mean of each experiment with standard errors.

    Article Snippet: As positive controls, a rabbit polyclonal anti-MERS-CoV spike protein antibody (Sino biological Inc., Beijing, China) for MERS-CoV, a mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany) for HCoV-229E, a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA) for HCoV-OC43, a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological Inc., Beijing, China) for hCoV-NL63, a human serum of a Korean SARS-CoV-2 convalescent person were also incubated.

    Techniques: Activity Assay, Neutralization, Plaque Reduction Neutralization Test, Inhibition

    Binding of human mAbs to S1 and RBD of the MERS-CoV S protein. Binding specificity of the mAbs was assessed by ELISA with a soluble S-2P trimer, S1, RBD sub-domains, and S2-mFc. Among the 11 purified mAbs, six were specific to RBD and five were specific to S1 (non-RBD). RBD-specific mAbs bound to both RBD and S1 protein. No S2-specific mAbs (blue line) were identified. Data points represent the mean of three technical replicates with standard errors.

    Journal: PLoS ONE

    Article Title: Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus

    doi: 10.1371/journal.pone.0232757

    Figure Lengend Snippet: Binding of human mAbs to S1 and RBD of the MERS-CoV S protein. Binding specificity of the mAbs was assessed by ELISA with a soluble S-2P trimer, S1, RBD sub-domains, and S2-mFc. Among the 11 purified mAbs, six were specific to RBD and five were specific to S1 (non-RBD). RBD-specific mAbs bound to both RBD and S1 protein. No S2-specific mAbs (blue line) were identified. Data points represent the mean of three technical replicates with standard errors.

    Article Snippet: As positive controls, a rabbit polyclonal anti-MERS-CoV spike protein antibody (Sino biological Inc., Beijing, China) for MERS-CoV, a mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany) for HCoV-229E, a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA) for HCoV-OC43, a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological Inc., Beijing, China) for hCoV-NL63, a human serum of a Korean SARS-CoV-2 convalescent person were also incubated.

    Techniques: Binding Assay, Protein Binding, Enzyme-linked Immunosorbent Assay, Purification

    MERS-CoV RBD-specific mAb inhibits viral replication in mouse tissues. Four mice were treated with the KNIH90-F1 mAb (8 mg/kg) 24 h after MERS-CoV KNIH-002 strain lethal challenge (1 × 10 5 pfu/mouse) under therapeutic conditions. Moreover, four mice were infected with MERS-CoV 24 h after administration of the same amount of mAb under prophylactic conditions. At 4-days post-challenge, all mice were humanely sacrificed, and tissues (lung and brain) were collected for viral titer analysis by plaque assays (A) and real-time RT-PCR (B). Viral genome copies in tissues were determined by real-time RT-PCR targeting the Orf1a gene. Data represent the mean ± standard deviation. * P ≤ 0.05; ** P ≤ 0.005; *** P ≤ 0.0005.

    Journal: PLoS ONE

    Article Title: Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus

    doi: 10.1371/journal.pone.0232757

    Figure Lengend Snippet: MERS-CoV RBD-specific mAb inhibits viral replication in mouse tissues. Four mice were treated with the KNIH90-F1 mAb (8 mg/kg) 24 h after MERS-CoV KNIH-002 strain lethal challenge (1 × 10 5 pfu/mouse) under therapeutic conditions. Moreover, four mice were infected with MERS-CoV 24 h after administration of the same amount of mAb under prophylactic conditions. At 4-days post-challenge, all mice were humanely sacrificed, and tissues (lung and brain) were collected for viral titer analysis by plaque assays (A) and real-time RT-PCR (B). Viral genome copies in tissues were determined by real-time RT-PCR targeting the Orf1a gene. Data represent the mean ± standard deviation. * P ≤ 0.05; ** P ≤ 0.005; *** P ≤ 0.0005.

    Article Snippet: As positive controls, a rabbit polyclonal anti-MERS-CoV spike protein antibody (Sino biological Inc., Beijing, China) for MERS-CoV, a mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany) for HCoV-229E, a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA) for HCoV-OC43, a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological Inc., Beijing, China) for hCoV-NL63, a human serum of a Korean SARS-CoV-2 convalescent person were also incubated.

    Techniques: Mouse Assay, Infection, Quantitative RT-PCR, Standard Deviation

    Histopathological changes of the lungs and brains in MERS-CoV-infected mice. Six mice were treated with the KNIH90-F1 mAb (8 mg/kg) 24 h after lethal challenge. At 4- and 7-days post-challenge, all mice were sacrificed, and lungs (A) and brains (B) were collected. Tissue sections were stained with H E (magnification: 400×).

    Journal: PLoS ONE

    Article Title: Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus

    doi: 10.1371/journal.pone.0232757

    Figure Lengend Snippet: Histopathological changes of the lungs and brains in MERS-CoV-infected mice. Six mice were treated with the KNIH90-F1 mAb (8 mg/kg) 24 h after lethal challenge. At 4- and 7-days post-challenge, all mice were sacrificed, and lungs (A) and brains (B) were collected. Tissue sections were stained with H E (magnification: 400×).

    Article Snippet: As positive controls, a rabbit polyclonal anti-MERS-CoV spike protein antibody (Sino biological Inc., Beijing, China) for MERS-CoV, a mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany) for HCoV-229E, a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA) for HCoV-OC43, a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological Inc., Beijing, China) for hCoV-NL63, a human serum of a Korean SARS-CoV-2 convalescent person were also incubated.

    Techniques: Infection, Mouse Assay, Staining

    MERS-CoV RBD-specific mAb protects mice from lethal challenge. Five hDPP4 TG mice were intraperitoneally administered different doses of KNIH90-F1 24 h after intranasal inoculation with the MERS-CoV KNIH-002 strain (1 × 10 5 pfu/30 μl). Body weight change (A) and survival (B) were monitored for 14 days.

    Journal: PLoS ONE

    Article Title: Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus

    doi: 10.1371/journal.pone.0232757

    Figure Lengend Snippet: MERS-CoV RBD-specific mAb protects mice from lethal challenge. Five hDPP4 TG mice were intraperitoneally administered different doses of KNIH90-F1 24 h after intranasal inoculation with the MERS-CoV KNIH-002 strain (1 × 10 5 pfu/30 μl). Body weight change (A) and survival (B) were monitored for 14 days.

    Article Snippet: As positive controls, a rabbit polyclonal anti-MERS-CoV spike protein antibody (Sino biological Inc., Beijing, China) for MERS-CoV, a mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany) for HCoV-229E, a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA) for HCoV-OC43, a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological Inc., Beijing, China) for hCoV-NL63, a human serum of a Korean SARS-CoV-2 convalescent person were also incubated.

    Techniques: Mouse Assay

    Ability of mAbs to inhibit MERS-CoV S protein binding to DPP4-expressing cells. Inhibition of MERS-CoV S binding to DPP4 by D12 mAb was measured by image cytometry (A) and fluorescence intensity was analyzed by FlowJo histograms (B). As a control, a non-relevant isotype monoclonal Ab was used. Dose response curves of Log mAb concentration versus percent inhibition were generated for D12 (C), G2 (D), and G4 (E) mAbs. (C-E) Each dot represents duplicates. Error bars represent SEM.

    Journal: Journal of virological methods

    Article Title: A high-throughput inhibition assay to study MERS-CoV antibody interactions using image cytometry

    doi: 10.1016/j.jviromet.2018.11.009

    Figure Lengend Snippet: Ability of mAbs to inhibit MERS-CoV S protein binding to DPP4-expressing cells. Inhibition of MERS-CoV S binding to DPP4 by D12 mAb was measured by image cytometry (A) and fluorescence intensity was analyzed by FlowJo histograms (B). As a control, a non-relevant isotype monoclonal Ab was used. Dose response curves of Log mAb concentration versus percent inhibition were generated for D12 (C), G2 (D), and G4 (E) mAbs. (C-E) Each dot represents duplicates. Error bars represent SEM.

    Article Snippet: Subsequently, 100 μL of MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) was pipetted into each well, incubated for 60 min at RT, and washed twice.

    Techniques: Protein Binding, Expressing, Inhibition, Binding Assay, Cytometry, Fluorescence, Concentration Assay, Generated

    MERS-CoV S1 and S proteins binding to DPP4-expressing cells. Whole well fluorescence images of MERS-CoV S1 (A) and MERS-CoV S (B) proteins binding DPP4-expressing BHK21 cells. Both S1 monomer and S trimer proteins were stained with anti-MERSCoV S polyclonal AF488, demonstrating binding to DPP4-expressing cells in green. Analysis of the image-based fluorescence intensity data by FlowJo for MERS-CoV S1 monomer (C) and S trimer (D) shows the peak shift results from binding.

    Journal: Journal of virological methods

    Article Title: A high-throughput inhibition assay to study MERS-CoV antibody interactions using image cytometry

    doi: 10.1016/j.jviromet.2018.11.009

    Figure Lengend Snippet: MERS-CoV S1 and S proteins binding to DPP4-expressing cells. Whole well fluorescence images of MERS-CoV S1 (A) and MERS-CoV S (B) proteins binding DPP4-expressing BHK21 cells. Both S1 monomer and S trimer proteins were stained with anti-MERSCoV S polyclonal AF488, demonstrating binding to DPP4-expressing cells in green. Analysis of the image-based fluorescence intensity data by FlowJo for MERS-CoV S1 monomer (C) and S trimer (D) shows the peak shift results from binding.

    Article Snippet: Subsequently, 100 μL of MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) was pipetted into each well, incubated for 60 min at RT, and washed twice.

    Techniques: Binding Assay, Expressing, Fluorescence, Staining