mers cov s rabbit polyclonal antibodies Search Results


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  • 95
    Sino Biological rabbit polyclonal antibody
    A) <t>ELISA</t> response for two different <t>PAb</t> anti-SARS-CoV-2 1 μg/mL (Sinobiological and ProSci) towards two different Spike proteins coated at 2 ng/mL. B) Binding curve of colorimetric ELISA for MAb anti-SARS-CoV-2 ranging from 0.12 – 2 μg/mL. Coating of Spike protein: 2 ng/mL. C) Electrochemical response using the MBs-based assay using CB-based modified electrode (blue line) and bare electrode (black line). The mean value (n = 3) with the corresponding standard deviation was reported for each measurement. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Rabbit Polyclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibody - by Bioz Stars, 2021-07
    95/100 stars
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    94
    Sino Biological mers cov nucleocapsid protein antibody rabbit pab
    <t>MERS-CoV</t> loads in the swabs and tissues of immunised rhesus macaques following MERS-CoV infection detected by Real-time-PCR. a. Viral loads in swab samples at 1 dpi and 3 dpi. b. The viral loads in lung and trachea tissue 3 days after MERS-CoV infection. All of the detections were replicated for three times. M, H and L represent mock, high- and low-dose groups, respectively. * p
    Mers Cov Nucleocapsid Protein Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov nucleocapsid protein antibody rabbit pab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov nucleocapsid protein antibody rabbit pab - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    94
    Sino Biological rabbit polyclonal anti mers cov spike protein antibody
    Characterization of <t>anti-MERS-S2P</t> IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their <t>MERS-CoV</t> specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    Rabbit Polyclonal Anti Mers Cov Spike Protein Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti mers cov spike protein antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti mers cov spike protein antibody - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    A) ELISA response for two different PAb anti-SARS-CoV-2 1 μg/mL (Sinobiological and ProSci) towards two different Spike proteins coated at 2 ng/mL. B) Binding curve of colorimetric ELISA for MAb anti-SARS-CoV-2 ranging from 0.12 – 2 μg/mL. Coating of Spike protein: 2 ng/mL. C) Electrochemical response using the MBs-based assay using CB-based modified electrode (blue line) and bare electrode (black line). The mean value (n = 3) with the corresponding standard deviation was reported for each measurement. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biosensors & Bioelectronics

    Article Title: Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva

    doi: 10.1016/j.bios.2020.112686

    Figure Lengend Snippet: A) ELISA response for two different PAb anti-SARS-CoV-2 1 μg/mL (Sinobiological and ProSci) towards two different Spike proteins coated at 2 ng/mL. B) Binding curve of colorimetric ELISA for MAb anti-SARS-CoV-2 ranging from 0.12 – 2 μg/mL. Coating of Spike protein: 2 ng/mL. C) Electrochemical response using the MBs-based assay using CB-based modified electrode (blue line) and bare electrode (black line). The mean value (n = 3) with the corresponding standard deviation was reported for each measurement. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: In A the response of ELISA was reported testing the two PAb and two S proteins and demonstrating the better affinity between PAb belonging from Sinobiological, Germany and Recombinant Spike protein SARS-CoV, S1 subunit from Sinobiological, Germany, thus these reagents were selected for further studies.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Modification, Standard Deviation

    MERS-CoV loads in the swabs and tissues of immunised rhesus macaques following MERS-CoV infection detected by Real-time-PCR. a. Viral loads in swab samples at 1 dpi and 3 dpi. b. The viral loads in lung and trachea tissue 3 days after MERS-CoV infection. All of the detections were replicated for three times. M, H and L represent mock, high- and low-dose groups, respectively. * p

    Journal: EBioMedicine

    Article Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge

    doi: 10.1016/j.ebiom.2015.08.031

    Figure Lengend Snippet: MERS-CoV loads in the swabs and tissues of immunised rhesus macaques following MERS-CoV infection detected by Real-time-PCR. a. Viral loads in swab samples at 1 dpi and 3 dpi. b. The viral loads in lung and trachea tissue 3 days after MERS-CoV infection. All of the detections were replicated for three times. M, H and L represent mock, high- and low-dose groups, respectively. * p

    Article Snippet: A rabbit-serum-derived polyclonal antibody against nucleoprotein (Sino Biological Inc., cat: 100213-RP02) at 1:1000 dilution, was then incubated with the sections.

    Techniques: Infection, Real-time Polymerase Chain Reaction

    IHC staining of immunised rhesus macaque lung and trachea 3 days after MERS-CoV infection by a polyclonal antibody against nucleoprotein. a–c. IHC analysis of lung tissue. d–f. IHC staining of trachea tissue. Black arrows indicate the distribution of viral antigen. M, H and L represent mock, high- and low-dose groups, respectively.

    Journal: EBioMedicine

    Article Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge

    doi: 10.1016/j.ebiom.2015.08.031

    Figure Lengend Snippet: IHC staining of immunised rhesus macaque lung and trachea 3 days after MERS-CoV infection by a polyclonal antibody against nucleoprotein. a–c. IHC analysis of lung tissue. d–f. IHC staining of trachea tissue. Black arrows indicate the distribution of viral antigen. M, H and L represent mock, high- and low-dose groups, respectively.

    Article Snippet: A rabbit-serum-derived polyclonal antibody against nucleoprotein (Sino Biological Inc., cat: 100213-RP02) at 1:1000 dilution, was then incubated with the sections.

    Techniques: Immunohistochemistry, Staining, Infection

    Schematic diagram of the rRBD vaccination and MERS-CoV challenge schedule. Monkeys were immunised three times intramuscularly (i.m.), at intervals of 8 or 17 weeks, and inoculated intratracheally with hCoV-EMC at a dosage of 6.5 × 10 7 TCID 50 (red arrow). Monkeys were bled periodically at 2, 10, and 25 weeks (− 14 days), and at − 1 day, 1 day and 3 days. Additionally, nasal, oral and rectal swabs were collected at − 1 day, 1 day and 3 days. Chest X-rays were performed 1 day before inoculation, and at 1 and 3 dpi. Following euthanasia, lung, trachea, spleen and kidney specimens of challenged monkeys were acquired at 3 dpi for detection.

    Journal: EBioMedicine

    Article Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge

    doi: 10.1016/j.ebiom.2015.08.031

    Figure Lengend Snippet: Schematic diagram of the rRBD vaccination and MERS-CoV challenge schedule. Monkeys were immunised three times intramuscularly (i.m.), at intervals of 8 or 17 weeks, and inoculated intratracheally with hCoV-EMC at a dosage of 6.5 × 10 7 TCID 50 (red arrow). Monkeys were bled periodically at 2, 10, and 25 weeks (− 14 days), and at − 1 day, 1 day and 3 days. Additionally, nasal, oral and rectal swabs were collected at − 1 day, 1 day and 3 days. Chest X-rays were performed 1 day before inoculation, and at 1 and 3 dpi. Following euthanasia, lung, trachea, spleen and kidney specimens of challenged monkeys were acquired at 3 dpi for detection.

    Article Snippet: A rabbit-serum-derived polyclonal antibody against nucleoprotein (Sino Biological Inc., cat: 100213-RP02) at 1:1000 dilution, was then incubated with the sections.

    Techniques:

    Radiographic alterations and lung pathology. a. X-rays from rhesus macaques imaged prior to (− 1 day) and post-MERS-CoV inoculation (1 day and 3 days). Areas of interstitial infiltration, indicative of pneumonia, are highlighted (circle). b. Gross pathology of the lungs from necropsied animals at 3 dpi. Haemorrhage and necrosis indicated by the black circle in both high and low dose immunisation groups were small and local, which happened in the mock group were large and disperse. M, H and L represent the mock, high- and low-dose groups, respectively.

    Journal: EBioMedicine

    Article Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge

    doi: 10.1016/j.ebiom.2015.08.031

    Figure Lengend Snippet: Radiographic alterations and lung pathology. a. X-rays from rhesus macaques imaged prior to (− 1 day) and post-MERS-CoV inoculation (1 day and 3 days). Areas of interstitial infiltration, indicative of pneumonia, are highlighted (circle). b. Gross pathology of the lungs from necropsied animals at 3 dpi. Haemorrhage and necrosis indicated by the black circle in both high and low dose immunisation groups were small and local, which happened in the mock group were large and disperse. M, H and L represent the mock, high- and low-dose groups, respectively.

    Article Snippet: A rabbit-serum-derived polyclonal antibody against nucleoprotein (Sino Biological Inc., cat: 100213-RP02) at 1:1000 dilution, was then incubated with the sections.

    Techniques:

    Histopathological changes in the lungs and tracheas of immunised rhesus macaques following MERS-CoV challenge. Three days after MERS-CoV inoculation, all monkeys were euthanised. Tissues were collected and stained with haematoxylin and eosin (H E). a–f. Pathological changes in the lungs of immunised monkeys. The black triangle indicates acute interstitial pneumonia diffused in lung tissue. The unfilled arrow indicates the hyaline member resulting from effusion of fibrin. The black arrow indicates the influx of inflammatory cells. g–i. Pathological findings of tracheas in immunised rhesus macaques. The black arrow highlights the infiltration of inflammatory cells in the tunica mucosa bronchiorum. The unfilled arrow indicates epithelium impairment in tunica mucosa bronchiorum. M, H and L represent the mock, high- and low-dose groups, respectively.

    Journal: EBioMedicine

    Article Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge

    doi: 10.1016/j.ebiom.2015.08.031

    Figure Lengend Snippet: Histopathological changes in the lungs and tracheas of immunised rhesus macaques following MERS-CoV challenge. Three days after MERS-CoV inoculation, all monkeys were euthanised. Tissues were collected and stained with haematoxylin and eosin (H E). a–f. Pathological changes in the lungs of immunised monkeys. The black triangle indicates acute interstitial pneumonia diffused in lung tissue. The unfilled arrow indicates the hyaline member resulting from effusion of fibrin. The black arrow indicates the influx of inflammatory cells. g–i. Pathological findings of tracheas in immunised rhesus macaques. The black arrow highlights the infiltration of inflammatory cells in the tunica mucosa bronchiorum. The unfilled arrow indicates epithelium impairment in tunica mucosa bronchiorum. M, H and L represent the mock, high- and low-dose groups, respectively.

    Article Snippet: A rabbit-serum-derived polyclonal antibody against nucleoprotein (Sino Biological Inc., cat: 100213-RP02) at 1:1000 dilution, was then incubated with the sections.

    Techniques: Staining

    Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Article Snippet: Then, human anti-MERS-S2P antibodies (S2A3, S2A6, and S2D5) and a rabbit polyclonal anti-MERS-CoV spike protein antibody, used as a positive control (Sino biological), in TBS (0.1% Triton X-100, 1% BSA in PBS) solution were incubated with the Vero cells infected with MERS-CoV.

    Techniques: Immunofluorescence, Infection, Size-exclusion Chromatography, SPR Assay, Chromatin Immunoprecipitation, Binding Assay

    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Article Snippet: Then, human anti-MERS-S2P antibodies (S2A3, S2A6, and S2D5) and a rabbit polyclonal anti-MERS-CoV spike protein antibody, used as a positive control (Sino biological), in TBS (0.1% Triton X-100, 1% BSA in PBS) solution were incubated with the Vero cells infected with MERS-CoV.

    Techniques: Clone Assay, Enzyme-linked Immunosorbent Assay

    Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Article Snippet: Then, human anti-MERS-S2P antibodies (S2A3, S2A6, and S2D5) and a rabbit polyclonal anti-MERS-CoV spike protein antibody, used as a positive control (Sino biological), in TBS (0.1% Triton X-100, 1% BSA in PBS) solution were incubated with the Vero cells infected with MERS-CoV.

    Techniques: Enzyme-linked Immunosorbent Assay, Sandwich ELISA