Journal: Journal of Virology
Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus
Figure Lengend Snippet: Histopathological changes in the lungs of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. Representative images of lungs from hDPP4 +/− transgenic mouse line 2 on days 1, 3, 5, 7, 14, and 35 postinoculation. Mild but progressive interstitial infiltration was seen within 7 days postinoculation (dpi) (left column, A, D, G, J, M, and P). IHC staining of sequential sections revealed abundant MERS-CoV antigen-positive cells in affected areas (middle column, B, E, H, K, N, and Q). Severe inflammation, with many mononuclear cells in the alveolar spaces and regenerated type II pneumocytes in the alveolar wall, was observed within 7 days p.i. (right column, C, F, I, L, O, and R). Scale bars: 100 μm (left and middle columns), 50 μm (right column), and 20 μm (insets of middle column). HE, hematoxylin and eosin staining; IHC, immunohistochemistry using an anti-MERS-CoV nucleocapsid protein polyclonal antibody; Ag, antigen.
Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).
Techniques: Transgenic Assay, Mouse Assay, Immunohistochemistry, Staining