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    Sino Biological mers cov nucleocapsid protein antibody rabbit pab
    Mers Cov Nucleocapsid Protein Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov nucleocapsid protein antibody rabbit pab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov nucleocapsid protein antibody rabbit pab - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    94
    Sino Biological mers cov nucleocapsid protein rabbit antibody
    In vivo replication of different <t>MERS-CoV</t> strains. hDPP4 mice were inoculated I.N. with 10 3 TCID 50 MERS-CoV. Four mice were euthanized on D3, and the remaining 6 mice were monitored for survival. A.) Relative weight loss of hDPP4 mice. B.) Survival of hDPP4 mice. C.) Oropharyngeal shedding of MERS-CoV as measured via UpE qRT-PCR. D.) Area under the curve of oropharyngeal MERS-CoV shedding per virus strain. E.) Viral load in lung tissue obtained from mice euthanized at D3. F.) Viral mRNA load in lung tissue obtained from mice euthanized at D3. G.) Lung tissue were stained for MERS-CoV antigen and % of positive pixels was quantified. Statistical significance was compared using an unpaired two-tailed Student’s t-test. P-value = *
    Mers Cov Nucleocapsid Protein Rabbit Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov nucleocapsid protein rabbit antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov nucleocapsid protein rabbit antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    94
    Sino Biological rabbit anti mers cov nucleocapsid antibody
    Susceptibility of adult human dipeptidyl peptidase 4 (hDPP4)-transgenic mice to <t>MERS-CoV</t> infection. Tg2, hDPP4 +/− transgenic mouse line 2; non-Tg, hDPP4 −/− mouse. (A) The body weight of 25-week-old mice was monitored daily after intranasal inoculation with MERS-CoV ( n = 6 Tg2 mice and n = 7 non-Tg mice). *, P
    Rabbit Anti Mers Cov Nucleocapsid Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mers cov nucleocapsid antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mers cov nucleocapsid antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    In vivo replication of different MERS-CoV strains. hDPP4 mice were inoculated I.N. with 10 3 TCID 50 MERS-CoV. Four mice were euthanized on D3, and the remaining 6 mice were monitored for survival. A.) Relative weight loss of hDPP4 mice. B.) Survival of hDPP4 mice. C.) Oropharyngeal shedding of MERS-CoV as measured via UpE qRT-PCR. D.) Area under the curve of oropharyngeal MERS-CoV shedding per virus strain. E.) Viral load in lung tissue obtained from mice euthanized at D3. F.) Viral mRNA load in lung tissue obtained from mice euthanized at D3. G.) Lung tissue were stained for MERS-CoV antigen and % of positive pixels was quantified. Statistical significance was compared using an unpaired two-tailed Student’s t-test. P-value = *

    Journal: bioRxiv

    Article Title: Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 - 2018

    doi: 10.1101/2021.02.11.429193

    Figure Lengend Snippet: In vivo replication of different MERS-CoV strains. hDPP4 mice were inoculated I.N. with 10 3 TCID 50 MERS-CoV. Four mice were euthanized on D3, and the remaining 6 mice were monitored for survival. A.) Relative weight loss of hDPP4 mice. B.) Survival of hDPP4 mice. C.) Oropharyngeal shedding of MERS-CoV as measured via UpE qRT-PCR. D.) Area under the curve of oropharyngeal MERS-CoV shedding per virus strain. E.) Viral load in lung tissue obtained from mice euthanized at D3. F.) Viral mRNA load in lung tissue obtained from mice euthanized at D3. G.) Lung tissue were stained for MERS-CoV antigen and % of positive pixels was quantified. Statistical significance was compared using an unpaired two-tailed Student’s t-test. P-value = *

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: In Vivo, Mouse Assay, Quantitative RT-PCR, Staining, Two Tailed Test

    Phylogenetic tree of MERS-CoV strains. Maximum likelihood tree of 446 full MERS CoV genomes showing distribution of isolates used in this study. Human-derived MERS-CoV isolates used in this study are highlighted in red, camel-derived MERS-CoV isolates are highlighted in blue. Phylogenetic tree reconstructed with PhyML and rooted at the midpoint.

    Journal: bioRxiv

    Article Title: Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 - 2018

    doi: 10.1101/2021.02.11.429193

    Figure Lengend Snippet: Phylogenetic tree of MERS-CoV strains. Maximum likelihood tree of 446 full MERS CoV genomes showing distribution of isolates used in this study. Human-derived MERS-CoV isolates used in this study are highlighted in red, camel-derived MERS-CoV isolates are highlighted in blue. Phylogenetic tree reconstructed with PhyML and rooted at the midpoint.

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Derivative Assay

    Stability of MERS-CoV strains on surfaces and in aerosols compared to SARS-CoV-2. A.) 50 µl of MERS-CoV or SARS-CoV-2 was spread on surface, either polypropylene, stainless steel, copper or silver. 1 mL of DMEM was added at T=0, 1, 24, 48 or 72 hours and titrated. B.) MERS-CoV or SARS-CoV-2 containing aerosols were sprayed into the Goldberg drum, and samples were taken at T=0, 30, 60, 120 and 180 minutes and titrated. Linear regression was calculated per virus and displayed in the graph as a line. A-B.) Statistically significant differences between EMC/12 and other strains were calculated using an unpaired Student’s two-tailed t-test corrected for multiple comparisons via Bonferroni. Dotted line = limit of detection; p-values = *

    Journal: bioRxiv

    Article Title: Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 - 2018

    doi: 10.1101/2021.02.11.429193

    Figure Lengend Snippet: Stability of MERS-CoV strains on surfaces and in aerosols compared to SARS-CoV-2. A.) 50 µl of MERS-CoV or SARS-CoV-2 was spread on surface, either polypropylene, stainless steel, copper or silver. 1 mL of DMEM was added at T=0, 1, 24, 48 or 72 hours and titrated. B.) MERS-CoV or SARS-CoV-2 containing aerosols were sprayed into the Goldberg drum, and samples were taken at T=0, 30, 60, 120 and 180 minutes and titrated. Linear regression was calculated per virus and displayed in the graph as a line. A-B.) Statistically significant differences between EMC/12 and other strains were calculated using an unpaired Student’s two-tailed t-test corrected for multiple comparisons via Bonferroni. Dotted line = limit of detection; p-values = *

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Two Tailed Test

    Susceptibility of adult human dipeptidyl peptidase 4 (hDPP4)-transgenic mice to MERS-CoV infection. Tg2, hDPP4 +/− transgenic mouse line 2; non-Tg, hDPP4 −/− mouse. (A) The body weight of 25-week-old mice was monitored daily after intranasal inoculation with MERS-CoV ( n = 6 Tg2 mice and n = 7 non-Tg mice). *, P

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Susceptibility of adult human dipeptidyl peptidase 4 (hDPP4)-transgenic mice to MERS-CoV infection. Tg2, hDPP4 +/− transgenic mouse line 2; non-Tg, hDPP4 −/− mouse. (A) The body weight of 25-week-old mice was monitored daily after intranasal inoculation with MERS-CoV ( n = 6 Tg2 mice and n = 7 non-Tg mice). *, P

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Transgenic Assay, Mouse Assay, Infection

    Histopathological changes in the lungs of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. Representative images of lungs from hDPP4 +/− transgenic mouse line 2 on days 1, 3, 5, 7, 14, and 35 postinoculation. Mild but progressive interstitial infiltration was seen within 7 days postinoculation (dpi) (left column, A, D, G, J, M, and P). IHC staining of sequential sections revealed abundant MERS-CoV antigen-positive cells in affected areas (middle column, B, E, H, K, N, and Q). Severe inflammation, with many mononuclear cells in the alveolar spaces and regenerated type II pneumocytes in the alveolar wall, was observed within 7 days p.i. (right column, C, F, I, L, O, and R). Scale bars: 100 μm (left and middle columns), 50 μm (right column), and 20 μm (insets of middle column). HE, hematoxylin and eosin staining; IHC, immunohistochemistry using an anti-MERS-CoV nucleocapsid protein polyclonal antibody; Ag, antigen.

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Histopathological changes in the lungs of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. Representative images of lungs from hDPP4 +/− transgenic mouse line 2 on days 1, 3, 5, 7, 14, and 35 postinoculation. Mild but progressive interstitial infiltration was seen within 7 days postinoculation (dpi) (left column, A, D, G, J, M, and P). IHC staining of sequential sections revealed abundant MERS-CoV antigen-positive cells in affected areas (middle column, B, E, H, K, N, and Q). Severe inflammation, with many mononuclear cells in the alveolar spaces and regenerated type II pneumocytes in the alveolar wall, was observed within 7 days p.i. (right column, C, F, I, L, O, and R). Scale bars: 100 μm (left and middle columns), 50 μm (right column), and 20 μm (insets of middle column). HE, hematoxylin and eosin staining; IHC, immunohistochemistry using an anti-MERS-CoV nucleocapsid protein polyclonal antibody; Ag, antigen.

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Transgenic Assay, Mouse Assay, Immunohistochemistry, Staining

    Identification of cells infiltrating the lung of Tg2 mice infected with MERS-CoV. Representative images of lungs from 10-week-old (young) and 25-week-old (adult) hDPP4 +/− transgenic mice (line 2) on day 7 postinoculation (p.i.). Infiltrating cells were positive for Iba-1 (green) or CD3 (brown). Bar, 20 μm.: Hematoxylin and eosin staining (HE) was used for the images in the upper panels, and anti-Iba-1 polyclonal antibody and anti-CD3 monoclonal antibody were used for IHC in the lower panels.

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Identification of cells infiltrating the lung of Tg2 mice infected with MERS-CoV. Representative images of lungs from 10-week-old (young) and 25-week-old (adult) hDPP4 +/− transgenic mice (line 2) on day 7 postinoculation (p.i.). Infiltrating cells were positive for Iba-1 (green) or CD3 (brown). Bar, 20 μm.: Hematoxylin and eosin staining (HE) was used for the images in the upper panels, and anti-Iba-1 polyclonal antibody and anti-CD3 monoclonal antibody were used for IHC in the lower panels.

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Mouse Assay, Infection, Transgenic Assay, Staining, Immunohistochemistry

    Cytokine and chemokine levels and expression of type I interferon (IFN) genes in the lungs of Tg2 mice infected with MERS-CoV. Cytokine and chemokine levels in lung samples from 10-week-old (A) and 25-week-old (B) mice are shown. Tg2 mice were inoculated with MERS-CoV or cell culture medium containing 2% FBS. Lungs were collected at the indicated times post-viral inoculation ( n = 3 to 4 mice per time point). Data represent the means ± standard deviations. A dotted line denotes the detection limit of the assay. *, P

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Cytokine and chemokine levels and expression of type I interferon (IFN) genes in the lungs of Tg2 mice infected with MERS-CoV. Cytokine and chemokine levels in lung samples from 10-week-old (A) and 25-week-old (B) mice are shown. Tg2 mice were inoculated with MERS-CoV or cell culture medium containing 2% FBS. Lungs were collected at the indicated times post-viral inoculation ( n = 3 to 4 mice per time point). Data represent the means ± standard deviations. A dotted line denotes the detection limit of the assay. *, P

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Mouse Assay, Infection, Cell Culture

    Histopathological changes in the brain of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. (A, D, and G) Sagittal sections of the head, including the nasal cavity, olfactory bulb, and brain, of a Tg2 mouse infected with MERS-CoV (images taken at 3, 7, and 35 days p.i. [dpi]). Right panels show the brain cortex from samples from panels A, D, and G, respectively, with hematoxylin and eosin staining (B, E, and F) and immunohistochemical analysis of MERS-CoV antigen (C, F, I). Neither lesions nor MERS-CoV antigen-positive cells were detected in the brain. Scale bars, 1 mm (A, D, and G) and 20 μm (B, C, E, F, H, and I).

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Histopathological changes in the brain of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. (A, D, and G) Sagittal sections of the head, including the nasal cavity, olfactory bulb, and brain, of a Tg2 mouse infected with MERS-CoV (images taken at 3, 7, and 35 days p.i. [dpi]). Right panels show the brain cortex from samples from panels A, D, and G, respectively, with hematoxylin and eosin staining (B, E, and F) and immunohistochemical analysis of MERS-CoV antigen (C, F, I). Neither lesions nor MERS-CoV antigen-positive cells were detected in the brain. Scale bars, 1 mm (A, D, and G) and 20 μm (B, C, E, F, H, and I).

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Transgenic Assay, Mouse Assay, Infection, Staining, Immunohistochemistry

    Double-immunofluorescence images taken at 1 day p.i. showing human dipeptidyl peptidase 4 (hDPP4) (green) and MERS-CoV antigen (red) in the lungs of Tg2 mice infected with MERS-CoV. Viral antigen-positive cells in the lungs were hDPP4-positive bronchiolar epithelial cells (upper panels) and alveolar epithelial cells (lower panels). Original magnification, ×600.

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Double-immunofluorescence images taken at 1 day p.i. showing human dipeptidyl peptidase 4 (hDPP4) (green) and MERS-CoV antigen (red) in the lungs of Tg2 mice infected with MERS-CoV. Viral antigen-positive cells in the lungs were hDPP4-positive bronchiolar epithelial cells (upper panels) and alveolar epithelial cells (lower panels). Original magnification, ×600.

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Immunofluorescence, Mouse Assay, Infection

    Histopathological changes in the lungs of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. Representative histopathological images of the lungs from 25-week-old Tg2 mice at 1, 3, 5, 7, 14, and 35 days post-MERS-CoV infection. Images in the left (A, D, G, J, M, and P) and right (C, F, I, L, O, and R) columns show time-dependent recruitment of inflammatory cells to the lung. Marked inflammatory cell infiltration was noted at 7 days postinoculation (dpi) in panels J and L. Images in the middle column (B, E, H, K, N, and Q) show immunohistochemical staining for MERS-CoV antigen (Ag). Scale bars: 100 μm (left and middle columns), 50 μm (right column), and 20 μm (insets of middle column). HE, hematoxylin and eosin staining; IHC, immunohistochemistry using an anti-MERS-CoV nucleocapsid protein polyclonal antibody.

    Journal: Journal of Virology

    Article Title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus

    doi: 10.1128/JVI.01818-18

    Figure Lengend Snippet: Histopathological changes in the lungs of human dipeptidyl peptidase 4 (hDPP4)-transgenic mice inoculated with MERS-CoV. Representative histopathological images of the lungs from 25-week-old Tg2 mice at 1, 3, 5, 7, 14, and 35 days post-MERS-CoV infection. Images in the left (A, D, G, J, M, and P) and right (C, F, I, L, O, and R) columns show time-dependent recruitment of inflammatory cells to the lung. Marked inflammatory cell infiltration was noted at 7 days postinoculation (dpi) in panels J and L. Images in the middle column (B, E, H, K, N, and Q) show immunohistochemical staining for MERS-CoV antigen (Ag). Scale bars: 100 μm (left and middle columns), 50 μm (right column), and 20 μm (insets of middle column). HE, hematoxylin and eosin staining; IHC, immunohistochemistry using an anti-MERS-CoV nucleocapsid protein polyclonal antibody.

    Article Snippet: For IHC, antigen retrieval of formalin-fixed mouse tissue sections was performed by autoclaving at 121°C for 10 min in retrieval solution at pH 6.0 (Nichirei, Tokyo, Japan). hDPP4 and MERS-CoV antigens were detected using a standard immunoperoxidase method and a goat anti-hDPP4 antibody (R & D Systems) and a rabbit anti-MERS-CoV nucleocapsid antibody (40068-RP01; Sino Biological, Inc., Beijing, China).

    Techniques: Transgenic Assay, Mouse Assay, Infection, Immunohistochemistry, Staining