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  • 97
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    Millipore mouse anti cnpase
    Immunohistochemistry analysis. Double immunostaining of OX42/CD163, <t>CNPase/CD44</t> and <t>CD86/NG2</t> in the lumbar spinal cord of control and EAE rats . a – d Double labeling for microglial cells marker (OX42) and M2 macrophages marker (CD163) in the white matter of control ( a ) and EAE experimental groups: 8, 11, and 18 DPI ( b – d ). Plus in b indicates the activated microglia expressing CD163. e – h Double labeling for oligodendrocyte marker (CNPase) and T lymphocyte marker (CD44) (see also the included high-power magnifications). CD44 is expressed by oligodendrocytes in control. Arrows in e indicate co-localization, although this expression changed at 8 DPI in f. i – l Double labeling for oligodendrocyte precursor cells (NG2) and M1 macrophages marker (CD86). The immunoreactive area of CD163 ( m ), CNPase ( n ), CD44 ( o ), and CD86 ( p ) in EAE rats compared to controls. Statistical analysis: one-way ANOVA and Dunnett’s multiple comparison test (* P
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    Merck & Co luminata western chemiluminescent hrp kit
    Immunohistochemistry analysis. Double immunostaining of OX42/CD163, <t>CNPase/CD44</t> and <t>CD86/NG2</t> in the lumbar spinal cord of control and EAE rats . a – d Double labeling for microglial cells marker (OX42) and M2 macrophages marker (CD163) in the white matter of control ( a ) and EAE experimental groups: 8, 11, and 18 DPI ( b – d ). Plus in b indicates the activated microglia expressing CD163. e – h Double labeling for oligodendrocyte marker (CNPase) and T lymphocyte marker (CD44) (see also the included high-power magnifications). CD44 is expressed by oligodendrocytes in control. Arrows in e indicate co-localization, although this expression changed at 8 DPI in f. i – l Double labeling for oligodendrocyte precursor cells (NG2) and M1 macrophages marker (CD86). The immunoreactive area of CD163 ( m ), CNPase ( n ), CD44 ( o ), and CD86 ( p ) in EAE rats compared to controls. Statistical analysis: one-way ANOVA and Dunnett’s multiple comparison test (* P
    Luminata Western Chemiluminescent Hrp Kit, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
    Immunohistochemistry analysis. Double immunostaining of OX42/CD163, <t>CNPase/CD44</t> and <t>CD86/NG2</t> in the lumbar spinal cord of control and EAE rats . a – d Double labeling for microglial cells marker (OX42) and M2 macrophages marker (CD163) in the white matter of control ( a ) and EAE experimental groups: 8, 11, and 18 DPI ( b – d ). Plus in b indicates the activated microglia expressing CD163. e – h Double labeling for oligodendrocyte marker (CNPase) and T lymphocyte marker (CD44) (see also the included high-power magnifications). CD44 is expressed by oligodendrocytes in control. Arrows in e indicate co-localization, although this expression changed at 8 DPI in f. i – l Double labeling for oligodendrocyte precursor cells (NG2) and M1 macrophages marker (CD86). The immunoreactive area of CD163 ( m ), CNPase ( n ), CD44 ( o ), and CD86 ( p ) in EAE rats compared to controls. Statistical analysis: one-way ANOVA and Dunnett’s multiple comparison test (* P
    Mouse Monoclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunohistochemistry analysis. Double immunostaining of OX42/CD163, CNPase/CD44 and CD86/NG2 in the lumbar spinal cord of control and EAE rats . a – d Double labeling for microglial cells marker (OX42) and M2 macrophages marker (CD163) in the white matter of control ( a ) and EAE experimental groups: 8, 11, and 18 DPI ( b – d ). Plus in b indicates the activated microglia expressing CD163. e – h Double labeling for oligodendrocyte marker (CNPase) and T lymphocyte marker (CD44) (see also the included high-power magnifications). CD44 is expressed by oligodendrocytes in control. Arrows in e indicate co-localization, although this expression changed at 8 DPI in f. i – l Double labeling for oligodendrocyte precursor cells (NG2) and M1 macrophages marker (CD86). The immunoreactive area of CD163 ( m ), CNPase ( n ), CD44 ( o ), and CD86 ( p ) in EAE rats compared to controls. Statistical analysis: one-way ANOVA and Dunnett’s multiple comparison test (* P

    Journal: Journal of Neuroinflammation

    Article Title: Cytokine and chemokine alterations in tissue, CSF, and plasma in early presymptomatic phase of experimental allergic encephalomyelitis (EAE), in a rat model of multiple sclerosis

    doi: 10.1186/s12974-016-0757-6

    Figure Lengend Snippet: Immunohistochemistry analysis. Double immunostaining of OX42/CD163, CNPase/CD44 and CD86/NG2 in the lumbar spinal cord of control and EAE rats . a – d Double labeling for microglial cells marker (OX42) and M2 macrophages marker (CD163) in the white matter of control ( a ) and EAE experimental groups: 8, 11, and 18 DPI ( b – d ). Plus in b indicates the activated microglia expressing CD163. e – h Double labeling for oligodendrocyte marker (CNPase) and T lymphocyte marker (CD44) (see also the included high-power magnifications). CD44 is expressed by oligodendrocytes in control. Arrows in e indicate co-localization, although this expression changed at 8 DPI in f. i – l Double labeling for oligodendrocyte precursor cells (NG2) and M1 macrophages marker (CD86). The immunoreactive area of CD163 ( m ), CNPase ( n ), CD44 ( o ), and CD86 ( p ) in EAE rats compared to controls. Statistical analysis: one-way ANOVA and Dunnett’s multiple comparison test (* P

    Article Snippet: The primary antibodies and dilutions used were as follows: rabbit anti-CD44 (1:100, Cluster of differentiation 44, Acris Antibodies, Inc, San Diego, USA), rabbit anti-CD163 (1:100, Cluster of differentiation 163, Bioss Inc, Woburn, USA), mouse anti-CD86 (1:250, Cluster of differentiation 86, Novus Biological Europe, Cambridge, UK), mouse anti-NF200 (1:200, Neurofilament, Sigma, Saint Louse, USA), rabbit anti CSF1 (1:100, colony-stimulating factor 1, Novus Biological), mouse anti-OX42 (1:250, AbD Serotec Inc., Raleigh, USA), mouse anti-NG2 (1:100, membrane-spanning chondroitin sulfate proteoglycan, Millipore, Merck S.p.a., Milan, Italy), mouse anti-CNPase (1:250, 2′, 3′-cyclic nucleotide 3′-phosphodiesterase, Millipore, Merck S.p.a., Milan, Italy), rabbit anti-GFAP (1:500, glial fibrillary acidic protein, Dako, Milan, Italy), and mouse anti-GFAP (1:500, Sigma, Saint Louse, USA).

    Techniques: Immunohistochemistry, Double Immunostaining, Labeling, Marker, Expressing