Journal: Dermatology Research and Practice

Article Title: Amniotic Membrane as a Scaffold for Melanocyte Transplantation in Patients with Stable Vitiligo

doi: 10.1155/2011/532139

Figure Lengend Snippet: Piece of amniotic membrane (a); immunohistochemistry for HMB-45 applied to cells cultured on slide. A large number of melanocytes can be observed (b); micrograph of cross-sectioned AM stained with HMB-45 after cell culture (c). Original magnification: (b, c) X100.

Article Snippet: Melanocyte basal medium (MBM, PromoCell, Heidelberg, Germany) without PMA (phorbol 12-myristate 13-acetate) nor bovine pituitary extract, supplemented with basic fibroblast growth factor (1 ng/mL), human recombinant stem cell factor (50 ng/mL), and human recombinant granulocyte/macrophage colony-stimulating factor (10 ng/mL) for 10–14 days, was used for the melanocyte culture.

Techniques: Membrane, Immunohistochemistry, Cell Culture, Staining

Journal: Dermatology Research and Practice

Article Title: Amniotic Membrane as a Scaffold for Melanocyte Transplantation in Patients with Stable Vitiligo

doi: 10.1155/2011/532139

Figure Lengend Snippet: Patient 1. Preoperatively (a); the achromic epidermis was removed using CO 2 laser, and AM carrying cultured melanocytes on the basement membrane surface was applied on the denuded area with one layer of Vaseline gauze. The amniotic membranes were fixed with steri-strips and covered with dry gauze and adhesive tape (b); postoperative photography (>90% repigmentation at 16 weeks) (c).

Article Snippet: Melanocyte basal medium (MBM, PromoCell, Heidelberg, Germany) without PMA (phorbol 12-myristate 13-acetate) nor bovine pituitary extract, supplemented with basic fibroblast growth factor (1 ng/mL), human recombinant stem cell factor (50 ng/mL), and human recombinant granulocyte/macrophage colony-stimulating factor (10 ng/mL) for 10–14 days, was used for the melanocyte culture.

Techniques: Cell Culture, Membrane, Adhesive