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  • 99
    Thermo Fisher 3730xl dna analyzer
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare megabace 1000
     Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of  Arabidopsis  sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled
    Megabace 1000, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare megabace 1000 dna analysis system
    Sequencing of <t>DNA</t> amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the DYEnamic ET terminator sequencing kit and run on a <t>MegaBACE</t> 1000 DNA sequencer.
    Megabace 1000 Dna Analysis System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare dyenamic et dye terminator cycle sequencing kit
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Dyenamic Et Dye Terminator Cycle Sequencing Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare dyenamic et dye terminator kit
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Dyenamic Et Dye Terminator Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare fragment profiler 1 2
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Fragment Profiler 1 2, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare gel band purification kit
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Gel Band Purification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 9485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo ta cloning kit
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Topo Ta Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare dna sequencer
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Dna Sequencer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Biotium agarose gel
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Agarose Gel, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher dyenamic et dye terminator kit
    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the <t>DYEnamic</t> ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.
    Dyenamic Et Dye Terminator Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


     Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of  Arabidopsis  sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled

    Journal: Nucleic Acids Research

    Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology

    doi: 10.1093/nar/gnh045

    Figure Lengend Snippet: Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of Arabidopsis sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled

    Article Snippet: SNPWave PCR products were desalted over Sephadex G-50 superfine columns in a 96-well plate format prior to detection by capillary electrophoresis on the MegaBACE 1000 (Amersham Biosciences).

    Techniques: Ligation, Amplification, Labeling

    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the DYEnamic ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.

    Journal: Genome Research

    Article Title: Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

    doi: 10.1101/gr.180501

    Figure Lengend Snippet: Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the DYEnamic ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.

    Article Snippet: The reaction was then ethanol precipitated and run on a MegaBACE 1000 DNA sequencer (Amersham Pharmacia Biotech) at 9 kV for 110 min.

    Techniques: Sequencing, Amplification, Plasmid Preparation

    Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the DYEnamic ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.

    Journal: Genome Research

    Article Title: Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

    doi: 10.1101/gr.180501

    Figure Lengend Snippet: Sequencing of DNA amplified from a saturated bacterial culture. A saturated culture of XL1-blue (Stratagene) containing a random library plasmid (2- to 3-kb inserts in pUC18, 2 μL) was amplified as described for 12 h (see Methods). The amplified DNA was treated with calf intestine phosphatase, heated to 95° for 3 min, and sequenced by using the DYEnamic ET terminator sequencing kit and run on a MegaBACE 1000 DNA sequencer.

    Article Snippet: Reactions were 30°C for 12 h. Reaction products were treated with calf intestine alkaline phosphatase (Amersham Pharmacia Biotech) at 37°C for 30 min and then heated to 95°C for 3 min. A 4-μL aliquot was used as a template for sequencing by using 5 pmoles of universal primer and a DYEnamic ET terminator sequencing kit (Amersham Pharmacia Biotech).

    Techniques: Sequencing, Amplification, Plasmid Preparation