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  • 99
    Thermo Fisher mega script t7 transcription kit
    Mega Script T7 Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega script t7 transcription kit/product/Thermo Fisher
    Average 99 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    mega script t7 transcription kit - by Bioz Stars, 2020-09
    99/100 stars
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    92
    Millipore mega 10
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega 10, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega 10/product/Millipore
    Average 92 stars, based on 22 article reviews
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    mega 10 - by Bioz Stars, 2020-09
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    98
    Qiagen plasmid mega kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Plasmid Mega Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 1050 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid mega kit/product/Qiagen
    Average 98 stars, based on 1050 article reviews
    Price from $9.99 to $1999.99
    plasmid mega kit - by Bioz Stars, 2020-09
    98/100 stars
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    99
    Qiagen endofree plasmid mega kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Endofree Plasmid Mega Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endofree plasmid mega kit/product/Qiagen
    Average 99 stars, based on 940 article reviews
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    endofree plasmid mega kit - by Bioz Stars, 2020-09
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    90
    Thermo Fisher mega shortscript t7 kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega Shortscript T7 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega shortscript t7 kit/product/Thermo Fisher
    Average 90 stars, based on 159 article reviews
    Price from $9.99 to $1999.99
    mega shortscript t7 kit - by Bioz Stars, 2020-09
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    88
    Thermo Fisher mega clear kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega Clear Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega clear kit/product/Thermo Fisher
    Average 88 stars, based on 222 article reviews
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    mega clear kit - by Bioz Stars, 2020-09
    88/100 stars
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    89
    Thermo Fisher mega script t7 kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega Script T7 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega script t7 kit/product/Thermo Fisher
    Average 89 stars, based on 207 article reviews
    Price from $9.99 to $1999.99
    mega script t7 kit - by Bioz Stars, 2020-09
    89/100 stars
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    89
    Thermo Fisher mega script rnai kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega Script Rnai Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega script rnai kit/product/Thermo Fisher
    Average 89 stars, based on 141 article reviews
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    mega script rnai kit - by Bioz Stars, 2020-09
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    88
    Thermo Fisher mega shortscript kit
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega Shortscript Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega shortscript kit/product/Thermo Fisher
    Average 88 stars, based on 171 article reviews
    Price from $9.99 to $1999.99
    mega shortscript kit - by Bioz Stars, 2020-09
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    90
    Olympus mega view iii camera
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega View Iii Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 90/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega view iii camera/product/Olympus
    Average 90 stars, based on 222 article reviews
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    mega view iii camera - by Bioz Stars, 2020-09
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    91
    Illumina Inc multi ethnic genotyping array mega
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Multi Ethnic Genotyping Array Mega, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multi ethnic genotyping array mega/product/Illumina Inc
    Average 91 stars, based on 22 article reviews
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    multi ethnic genotyping array mega - by Bioz Stars, 2020-09
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    88
    Soft Imaging System GmbH mega view ii charge coupled device camera
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega View Ii Charge Coupled Device Camera, supplied by Soft Imaging System GmbH, used in various techniques. Bioz Stars score: 88/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega view ii charge coupled device camera/product/Soft Imaging System GmbH
    Average 88 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    mega view ii charge coupled device camera - by Bioz Stars, 2020-09
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    93
    Illumina Inc illumina mega array
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Illumina Mega Array, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina mega array/product/Illumina Inc
    Average 93 stars, based on 24 article reviews
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    illumina mega array - by Bioz Stars, 2020-09
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    89
    Bioedit Company mega 6
    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) <t>MEGA-10,</t> ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.
    Mega 6, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 89/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega 6/product/Bioedit Company
    Average 89 stars, based on 118 article reviews
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    mega 6 - by Bioz Stars, 2020-09
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    92
    Pacific Biosciences mega reads
    Construction of super-reads and <t>mega-reads</t> from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. <t>PacBio</t> reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).
    Mega Reads, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega reads/product/Pacific Biosciences
    Average 92 stars, based on 18 article reviews
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    mega reads - by Bioz Stars, 2020-09
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    89
    Olympus mega view iii ccd camera
    Construction of super-reads and <t>mega-reads</t> from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. <t>PacBio</t> reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).
    Mega View Iii Ccd Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 89/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mega view iii ccd camera/product/Olympus
    Average 89 stars, based on 115 article reviews
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    mega view iii ccd camera - by Bioz Stars, 2020-09
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    99
    Qiagen plasmid plus mega kit
    Construction of super-reads and <t>mega-reads</t> from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. <t>PacBio</t> reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).
    Plasmid Plus Mega Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid plus mega kit/product/Qiagen
    Average 99 stars, based on 60 article reviews
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    plasmid plus mega kit - by Bioz Stars, 2020-09
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    93
    Qiagen qiafilter plasmid mega kit
    Construction of super-reads and <t>mega-reads</t> from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. <t>PacBio</t> reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).
    Qiafilter Plasmid Mega Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiafilter plasmid mega kit/product/Qiagen
    Average 93 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    qiafilter plasmid mega kit - by Bioz Stars, 2020-09
    93/100 stars
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    91
    TaKaRa adeno x mega purification kit
    Construction of super-reads and <t>mega-reads</t> from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. <t>PacBio</t> reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).
    Adeno X Mega Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adeno x mega purification kit/product/TaKaRa
    Average 91 stars, based on 259 article reviews
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    adeno x mega purification kit - by Bioz Stars, 2020-09
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    Image Search Results


    Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) MEGA-10, ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.

    Journal: Nature Communications

    Article Title: Turning bubbles on and off during boiling using charged surfactants

    doi: 10.1038/ncomms9599

    Figure Lengend Snippet: Boiling curves showing tunability of charged surfactants. Plain DI water (black), −0.1 V (red) and −2.0 V (blue) boiling curves for 2.6 mM ( a ) NaBr, ( b ) MEGA-10, ( c ) SDS and ( d ) DTAB. Time averaged data points from an individual boiling experiment with error bars (2 standard deviations in data spread from time averaging) and moving averages (lines) are shown. Boiling was not affected by voltage for ( a ) NaBr and ( b ) MEGA-10. For ( c ) negatively charged SDS, the boiling curve at −2.0 V was right-shifted with higher CHF compared with −0.1 V. For ( d ) positively charged DTAB, the boiling curve at −2.0 V was left-shifted with lower CHF compared with −0.1 V. The maximum change in HTC (tunability) at constant q ′′ (horizontal arrows) and constant superheat (vertical arrows) are shown for SDS and DTAB.

    Article Snippet: Surfactant preparation NaBr, MEGA-10, S10S, SDS and DTAB (all from Sigma) were dissolved at a concentration of 173 mM in DI water.

    Techniques:

    Construction of super-reads and mega-reads from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. PacBio reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).

    Journal: GigaScience

    Article Title: An improved assembly of the loblolly pine mega-genome using long-read single-molecule sequencing

    doi: 10.1093/gigascience/giw016

    Figure Lengend Snippet: Construction of super-reads and mega-reads from Illumina reads. Illumina reads (top left) were used to build longer super-reads (green lines), which in turn were used to construct a database of all 15-mers in those reads. For P. taeda , each super-read replaced an average of ∼150 Illumina reads; Table 1 ) [ 5 ]. PacBio reads (purple lines) and super-reads were then aligned using the 15-mer database. Inconsistent super-reads are shown as kinked lines; these were discarded and the remaining super-reads were merged, using the PacBio reads as templates, to produce mega-reads. The sequence of the mega-reads was thus derived entirely from the low-error-rate super-reads, not from the raw PacBio reads (figure modified from Zimin et al. [ 7 ]).

    Article Snippet: To produce the mega-reads from the PacBio data, and then assemble the mega-reads into contigs, we used the MaSuRCA assembler [ ], which has been updated to handle very long reads.

    Techniques: Construct, Sequencing, Derivative Assay, Modification