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    Millipore mix mda mb 231
    <t>MDA-MB-231</t> and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 130 µg/mL FN. ( A , E ) Untreated MDA-MB-231 cells displayed three different cell phenotypes: more numerous globular cells, large flattened-polygonal cells and fewer elongated-fusiform ones. Very few short filopodia and only few intercellular TNTs are observable. ( B , F ) With E2 treatment, MDA-MB-231 cells show isolated or grouped globular cells usually growing in more than one layer on more numerous elongated-fusiform and flattened-polygonal ones. Long conical filopodia and intercellular TNTs are visible. ( C , G ) After treatment with AG1478, MDA-MB-231 cells appear more grouped and include globular phenotypes growing on fewer elongated-fusiform and increased very large flattened-polygonal ones vs. control group. Few filopodia and TNTs are also visible. ( D , H ) With mix treatment, MDA-MB-231 cells appear grouped and show globular, large flattened-polygonal and elongated-fusiform shapes; long filopodia and TNTs. ( I , M ) Untreated shERβ MDA-MB-231 cells mainly show grouped globular cells but some isolated globular cells with few microvilli, and few EVs are also present. No filopodia are observable, whereas few short TNTs connect adjacent cells. ( J , N ) After E2 treatment, shERβ MDA-MB-231 cells display isolated and grouped globular cells growing in different layers on flattened-polygonal and isolated elongated-fusiform cells. Cells display microvilli and few EVs, long filopodia and TNTs. ( K , O ) After AG1478 treatment, the shERβ MDA-MB-231 cells include many large flattened-polygonal, globular and elongated-fusiform cells which appeared a little more grouped when compared to the E2 group. Few microvilli and few EVs, long filopodia and TNTs are observable. ( L , P ) The same cells treated with both E2 and AG1478 (mix) comprise globular, flattened-polygonal and elongated-fusiform cells equally represented and exhibiting few EVs and few microvilli, long filopodia and TNTs. Bar = 0.1 mm.
    Mix Mda Mb 231, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mix mda mb 231/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mix mda mb 231 - by Bioz Stars, 2021-07
    97/100 stars
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    99
    Qiagen mda reaction mix
    <t>MDA-MB-231</t> and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 130 µg/mL FN. ( A , E ) Untreated MDA-MB-231 cells displayed three different cell phenotypes: more numerous globular cells, large flattened-polygonal cells and fewer elongated-fusiform ones. Very few short filopodia and only few intercellular TNTs are observable. ( B , F ) With E2 treatment, MDA-MB-231 cells show isolated or grouped globular cells usually growing in more than one layer on more numerous elongated-fusiform and flattened-polygonal ones. Long conical filopodia and intercellular TNTs are visible. ( C , G ) After treatment with AG1478, MDA-MB-231 cells appear more grouped and include globular phenotypes growing on fewer elongated-fusiform and increased very large flattened-polygonal ones vs. control group. Few filopodia and TNTs are also visible. ( D , H ) With mix treatment, MDA-MB-231 cells appear grouped and show globular, large flattened-polygonal and elongated-fusiform shapes; long filopodia and TNTs. ( I , M ) Untreated shERβ MDA-MB-231 cells mainly show grouped globular cells but some isolated globular cells with few microvilli, and few EVs are also present. No filopodia are observable, whereas few short TNTs connect adjacent cells. ( J , N ) After E2 treatment, shERβ MDA-MB-231 cells display isolated and grouped globular cells growing in different layers on flattened-polygonal and isolated elongated-fusiform cells. Cells display microvilli and few EVs, long filopodia and TNTs. ( K , O ) After AG1478 treatment, the shERβ MDA-MB-231 cells include many large flattened-polygonal, globular and elongated-fusiform cells which appeared a little more grouped when compared to the E2 group. Few microvilli and few EVs, long filopodia and TNTs are observable. ( L , P ) The same cells treated with both E2 and AG1478 (mix) comprise globular, flattened-polygonal and elongated-fusiform cells equally represented and exhibiting few EVs and few microvilli, long filopodia and TNTs. Bar = 0.1 mm.
    Mda Reaction Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda reaction mix/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda reaction mix - by Bioz Stars, 2021-07
    99/100 stars
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    86
    Becton Dickinson mda mb 231 cells mixed
    ANGPTL4 enhances breast cancer cell metastasis to the lungs. ( A–C ) pBabe and pAngptl4 subclones of <t>MDA-MB-231</t> DKD cells were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pBabe ( C ). *, P
    Mda Mb 231 Cells Mixed, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 231 cells mixed/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 231 cells mixed - by Bioz Stars, 2021-07
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    98
    ATCC cell mixtures breast cancer cell lines mda mb 361
    Bland–Altman plot describing the agreement between tumor genome fraction (TGF) measures obtained with ichorCNA ( A ), control-FREEC ( B ) and the real tumor fractions (TFs). Differences between triplicate TGF measures and real TFs are separately reported for <t>MDA-MB-361</t> (red triangles) and MDA-MB-453 (green squares) as a function of the measures’ averages. Linear fit for each cell line is reported with intervals of confidence. Dashed lines from top to bottom represent the mean of difference plus two standard deviations, mean of difference and mean of difference minus two standard deviations.
    Cell Mixtures Breast Cancer Cell Lines Mda Mb 361, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell mixtures breast cancer cell lines mda mb 361/product/ATCC
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell mixtures breast cancer cell lines mda mb 361 - by Bioz Stars, 2021-07
    98/100 stars
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    Image Search Results


    MDA-MB-231 and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 130 µg/mL FN. ( A , E ) Untreated MDA-MB-231 cells displayed three different cell phenotypes: more numerous globular cells, large flattened-polygonal cells and fewer elongated-fusiform ones. Very few short filopodia and only few intercellular TNTs are observable. ( B , F ) With E2 treatment, MDA-MB-231 cells show isolated or grouped globular cells usually growing in more than one layer on more numerous elongated-fusiform and flattened-polygonal ones. Long conical filopodia and intercellular TNTs are visible. ( C , G ) After treatment with AG1478, MDA-MB-231 cells appear more grouped and include globular phenotypes growing on fewer elongated-fusiform and increased very large flattened-polygonal ones vs. control group. Few filopodia and TNTs are also visible. ( D , H ) With mix treatment, MDA-MB-231 cells appear grouped and show globular, large flattened-polygonal and elongated-fusiform shapes; long filopodia and TNTs. ( I , M ) Untreated shERβ MDA-MB-231 cells mainly show grouped globular cells but some isolated globular cells with few microvilli, and few EVs are also present. No filopodia are observable, whereas few short TNTs connect adjacent cells. ( J , N ) After E2 treatment, shERβ MDA-MB-231 cells display isolated and grouped globular cells growing in different layers on flattened-polygonal and isolated elongated-fusiform cells. Cells display microvilli and few EVs, long filopodia and TNTs. ( K , O ) After AG1478 treatment, the shERβ MDA-MB-231 cells include many large flattened-polygonal, globular and elongated-fusiform cells which appeared a little more grouped when compared to the E2 group. Few microvilli and few EVs, long filopodia and TNTs are observable. ( L , P ) The same cells treated with both E2 and AG1478 (mix) comprise globular, flattened-polygonal and elongated-fusiform cells equally represented and exhibiting few EVs and few microvilli, long filopodia and TNTs. Bar = 0.1 mm.

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: MDA-MB-231 and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 130 µg/mL FN. ( A , E ) Untreated MDA-MB-231 cells displayed three different cell phenotypes: more numerous globular cells, large flattened-polygonal cells and fewer elongated-fusiform ones. Very few short filopodia and only few intercellular TNTs are observable. ( B , F ) With E2 treatment, MDA-MB-231 cells show isolated or grouped globular cells usually growing in more than one layer on more numerous elongated-fusiform and flattened-polygonal ones. Long conical filopodia and intercellular TNTs are visible. ( C , G ) After treatment with AG1478, MDA-MB-231 cells appear more grouped and include globular phenotypes growing on fewer elongated-fusiform and increased very large flattened-polygonal ones vs. control group. Few filopodia and TNTs are also visible. ( D , H ) With mix treatment, MDA-MB-231 cells appear grouped and show globular, large flattened-polygonal and elongated-fusiform shapes; long filopodia and TNTs. ( I , M ) Untreated shERβ MDA-MB-231 cells mainly show grouped globular cells but some isolated globular cells with few microvilli, and few EVs are also present. No filopodia are observable, whereas few short TNTs connect adjacent cells. ( J , N ) After E2 treatment, shERβ MDA-MB-231 cells display isolated and grouped globular cells growing in different layers on flattened-polygonal and isolated elongated-fusiform cells. Cells display microvilli and few EVs, long filopodia and TNTs. ( K , O ) After AG1478 treatment, the shERβ MDA-MB-231 cells include many large flattened-polygonal, globular and elongated-fusiform cells which appeared a little more grouped when compared to the E2 group. Few microvilli and few EVs, long filopodia and TNTs are observable. ( L , P ) The same cells treated with both E2 and AG1478 (mix) comprise globular, flattened-polygonal and elongated-fusiform cells equally represented and exhibiting few EVs and few microvilli, long filopodia and TNTs. Bar = 0.1 mm.

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Multiple Displacement Amplification, Cell Culture, Isolation

    ( A ) MDA-MB-231 cells treated with E2 and cultivated on a Millipore filter coated by 130 µg/mL FN: Flattened-polygonal cells adhering to the collagen substrate are covered by globular and elongated-fusiform cells showing microvilli and EVs. Long conical-shaped filopodia (large arrows) including microvilli and EVs on their surface arise from elongated-fusiform cells. Bar = 10 µm. ( B ) shERβ MDA-MB-231 cells treated with both E2 and AG1478 (mix) and cultivated on a Millipore filter coated by 130 µg/mL FN: Globular and elongated-fusiform cells on the Millipore filter are detectable. Long filopodia (large arrows) developing from elongate cells and straight intercellular TNTs (narrow arrows) with EVs on their surface are visible. Bar = 10 µm.

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: ( A ) MDA-MB-231 cells treated with E2 and cultivated on a Millipore filter coated by 130 µg/mL FN: Flattened-polygonal cells adhering to the collagen substrate are covered by globular and elongated-fusiform cells showing microvilli and EVs. Long conical-shaped filopodia (large arrows) including microvilli and EVs on their surface arise from elongated-fusiform cells. Bar = 10 µm. ( B ) shERβ MDA-MB-231 cells treated with both E2 and AG1478 (mix) and cultivated on a Millipore filter coated by 130 µg/mL FN: Globular and elongated-fusiform cells on the Millipore filter are detectable. Long filopodia (large arrows) developing from elongate cells and straight intercellular TNTs (narrow arrows) with EVs on their surface are visible. Bar = 10 µm.

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Multiple Displacement Amplification

    EGFR/ERβ axis regulates functional properties of MDA-MB-231 and shERβ MDA-MB-231 breast cancer cells. ( A ) Collagen type I adhesion. ( B ) Cell invasion in collagen type I matrix. Treatments with AG1478 (2 μM) and/or E2 (10 nM) were performed for 24 h prior in MDA-MB-231 and shERβ MDA-MB-231 cells. Each bar represents mean ± SD values from triplicate samples. Statistically significant differences are indicated accordingly: * p

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: EGFR/ERβ axis regulates functional properties of MDA-MB-231 and shERβ MDA-MB-231 breast cancer cells. ( A ) Collagen type I adhesion. ( B ) Cell invasion in collagen type I matrix. Treatments with AG1478 (2 μM) and/or E2 (10 nM) were performed for 24 h prior in MDA-MB-231 and shERβ MDA-MB-231 cells. Each bar represents mean ± SD values from triplicate samples. Statistically significant differences are indicated accordingly: * p

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Functional Assay, Multiple Displacement Amplification

    ( A ) Untreated MDA-MB-231 cells cultivated on a Millipore filter coated by 3000 µg/mL type I collagen: Flattened-polygonal cells (F) adhering to the collagen substrate are covered by globular (G) and elongated-fusiform (E) cells exhibiting microvilli and EVs. Long conical-shaped filopodia (large arrows) showing microvilli and EVs on their surface originate from elongated-fusiform cells. Straight intercellular TNTs (narrow arrow) are also present. Bar = 10 µm. ( B ) shERβ MDA-MB-231 cells treated with both E2 and AG1478 (mix) and cultivated on a Millipore filter coated by 3.0 mg/mL type I collagen: Globular and elongated cells showing microvilli on the cytoplasmic surface gro w on flattened-polygonal cells just adhering to the collagen layer. A conical filopodia (large arrow) develops from an elongated-fusiform cell. Short straight intercellular TNTs (narrow arrows) are detectable. Bar = 10 µm.

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: ( A ) Untreated MDA-MB-231 cells cultivated on a Millipore filter coated by 3000 µg/mL type I collagen: Flattened-polygonal cells (F) adhering to the collagen substrate are covered by globular (G) and elongated-fusiform (E) cells exhibiting microvilli and EVs. Long conical-shaped filopodia (large arrows) showing microvilli and EVs on their surface originate from elongated-fusiform cells. Straight intercellular TNTs (narrow arrow) are also present. Bar = 10 µm. ( B ) shERβ MDA-MB-231 cells treated with both E2 and AG1478 (mix) and cultivated on a Millipore filter coated by 3.0 mg/mL type I collagen: Globular and elongated cells showing microvilli on the cytoplasmic surface gro w on flattened-polygonal cells just adhering to the collagen layer. A conical filopodia (large arrow) develops from an elongated-fusiform cell. Short straight intercellular TNTs (narrow arrows) are detectable. Bar = 10 µm.

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Multiple Displacement Amplification

    MDA-MB-231 and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 3000 µg/mL type I collagen. ( A , E ) Untreated MDA-MB-231 cells include globular and elongated-fusiform phenotypes growing on flattened-polygonal cells strongly adhering to the collagen layer. Intercellular TNTs and long filopodia mainly originating from elongated-fusiform cells are visible. ( B , F ) When compared to the untreated samples, the same cells treated with E2 show a slight increase of globular and elongated-fusiform cells on flattened-polygonal ones adhering to the collagen substrate. Horizontal long conical filopodia and short intercellular TNTs are present. ( C , G ) MDA-MB-231 cells treated with AG1478 still exhibited globular and elongated-fusiform phenotypes on slightly increased flattened-polygonal cells. Long filopodia and TNTs are visible. ( D , H ) After a mix (E2 and AG1478) treatment, the MDA-MB-231 cells show a layer of flattened-polygonal cells adhering to the collagen substrate and covered by slightly decreased globular and elongated-fusiform cells with both long filopodia and TNTs. ( I , M ) Untreated shERβ MDA-MB-231 cells mainly include a globular cell phenotype. Cells appear mainly grouped and are occasionally connected by short intercellular TNTs; no filopodia are detectable. ( J , N ) When the same cells were treated with E2, mainly isolated globular and elongated-fusiform cells grew on a layer of flattened-polygonal cells. Cells developed EVs and microvilli, long filopodia and TNTs vs. control group. ( K , O ) shERβ MDA-MB-231 cells treated with AG1478 include many globular cells and few elongated-fusiform cells, all covering the flattened-polygonal ones. Cells are in very tight contact with each other, are uniformly distributed and show a smoother surface with few microvilli and fewer EVs, if compared to control and E2 groups. Long filopodia and TNTs were detectable. ( L , P ) After both E2 and AG 1478 treatments (mix group), shERB MDA-MB-231 cells included few elongated-fusiform- and many globular-shaped cells growing grouped in more than one layer on the flattened-polygonal ones. Filopodia are detectable. Bar = 0.1 mm.

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: MDA-MB-231 and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 3000 µg/mL type I collagen. ( A , E ) Untreated MDA-MB-231 cells include globular and elongated-fusiform phenotypes growing on flattened-polygonal cells strongly adhering to the collagen layer. Intercellular TNTs and long filopodia mainly originating from elongated-fusiform cells are visible. ( B , F ) When compared to the untreated samples, the same cells treated with E2 show a slight increase of globular and elongated-fusiform cells on flattened-polygonal ones adhering to the collagen substrate. Horizontal long conical filopodia and short intercellular TNTs are present. ( C , G ) MDA-MB-231 cells treated with AG1478 still exhibited globular and elongated-fusiform phenotypes on slightly increased flattened-polygonal cells. Long filopodia and TNTs are visible. ( D , H ) After a mix (E2 and AG1478) treatment, the MDA-MB-231 cells show a layer of flattened-polygonal cells adhering to the collagen substrate and covered by slightly decreased globular and elongated-fusiform cells with both long filopodia and TNTs. ( I , M ) Untreated shERβ MDA-MB-231 cells mainly include a globular cell phenotype. Cells appear mainly grouped and are occasionally connected by short intercellular TNTs; no filopodia are detectable. ( J , N ) When the same cells were treated with E2, mainly isolated globular and elongated-fusiform cells grew on a layer of flattened-polygonal cells. Cells developed EVs and microvilli, long filopodia and TNTs vs. control group. ( K , O ) shERβ MDA-MB-231 cells treated with AG1478 include many globular cells and few elongated-fusiform cells, all covering the flattened-polygonal ones. Cells are in very tight contact with each other, are uniformly distributed and show a smoother surface with few microvilli and fewer EVs, if compared to control and E2 groups. Long filopodia and TNTs were detectable. ( L , P ) After both E2 and AG 1478 treatments (mix group), shERB MDA-MB-231 cells included few elongated-fusiform- and many globular-shaped cells growing grouped in more than one layer on the flattened-polygonal ones. Filopodia are detectable. Bar = 0.1 mm.

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Multiple Displacement Amplification, Cell Culture, Isolation

    MDA-MB-231 and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 200 µg/mL type I collagen. ( A ) Untreated MDA-MB-231 globular-shaped cells are grouped in clusters of a few cells showing long, thin filopodia and tunneling nanotubes (TNTs). ( B ) Most of MDA-MB-231 cells treated with E2 show a globular shape, but elongated-fusiform and flattened-polygonal cells are also observable. ( C ) Thin long filopodia are present similarly to untreated cells, but TNTs seem more numerous vs. control group MDA-MB-231 cells treated with AG1478 include globular, elongated-fusiform but a higher number of flattened-polygonal-shaped cells vs. control and E2 treated groups. Thin, long filopodia and TNTs are still detectable. ( D ) After both E2 and AG1478 treatment (mix group) MDA-MB-231 cells show globular, flattened-polygonal and few elongated-fusiform phenotypes. TNTs and many long, thin filopodia adhering to the collagen substrate and radially arising from cells give the cells a “spider” aspect. ( E ) Untreated shERβ MDA-MB-231 mainly includes smooth globular cells grouped in tight contact and showing short thin filopodia and few TNTs. ( F ) The same cells treated with E2 still exhibit a globular shape but appear in isolated groups of two or three cells showing longer filopodia vs. untreated control group. ( G ) After AG1478 treatment, the shERβ MDA-MB-231 globular-shaped cells again look grouped and show more contact one to each other. Fewer short and thin filopodia and intercellular TNTs are present. ( H ) After the combined treatment of E2 and AG1478, shERβ MDA-MB-231 cells look relatively grouped, displaying the same globular shape and show few short filopodia and TNTs. Bar = 0.1 mm.

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: MDA-MB-231 and shERβ MDA-MB-231 cells cultured on Millipore filter covered by 200 µg/mL type I collagen. ( A ) Untreated MDA-MB-231 globular-shaped cells are grouped in clusters of a few cells showing long, thin filopodia and tunneling nanotubes (TNTs). ( B ) Most of MDA-MB-231 cells treated with E2 show a globular shape, but elongated-fusiform and flattened-polygonal cells are also observable. ( C ) Thin long filopodia are present similarly to untreated cells, but TNTs seem more numerous vs. control group MDA-MB-231 cells treated with AG1478 include globular, elongated-fusiform but a higher number of flattened-polygonal-shaped cells vs. control and E2 treated groups. Thin, long filopodia and TNTs are still detectable. ( D ) After both E2 and AG1478 treatment (mix group) MDA-MB-231 cells show globular, flattened-polygonal and few elongated-fusiform phenotypes. TNTs and many long, thin filopodia adhering to the collagen substrate and radially arising from cells give the cells a “spider” aspect. ( E ) Untreated shERβ MDA-MB-231 mainly includes smooth globular cells grouped in tight contact and showing short thin filopodia and few TNTs. ( F ) The same cells treated with E2 still exhibit a globular shape but appear in isolated groups of two or three cells showing longer filopodia vs. untreated control group. ( G ) After AG1478 treatment, the shERβ MDA-MB-231 globular-shaped cells again look grouped and show more contact one to each other. Fewer short and thin filopodia and intercellular TNTs are present. ( H ) After the combined treatment of E2 and AG1478, shERβ MDA-MB-231 cells look relatively grouped, displaying the same globular shape and show few short filopodia and TNTs. Bar = 0.1 mm.

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Multiple Displacement Amplification, Cell Culture, Isolation

    EGFR inhibition affects the expression and activity levels of proteases in MDA-MB-231 and shERβ MDA-MB-231 breast cancer cells. ( A ) Quantitative RT-PCR analysis of MMP7 and MT1-MMP mRNA levels after 24 h without and with treatments (AG1478, E2 and mix). ( B , C ) MMP2/MMP9 gelatinolytic activities (as assayed by gelatin zymography) in MDA-MB-231 and shERβ MDA-MB-231 cells, before and after treatments (AG1478, E2, mix and 24 h). Each bar represents mean ± SD values from triplicate samples. Statistically significant differences are indicated accordingly: * p

    Journal: Cells

    Article Title: ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer

    doi: 10.3390/cells9102256

    Figure Lengend Snippet: EGFR inhibition affects the expression and activity levels of proteases in MDA-MB-231 and shERβ MDA-MB-231 breast cancer cells. ( A ) Quantitative RT-PCR analysis of MMP7 and MT1-MMP mRNA levels after 24 h without and with treatments (AG1478, E2 and mix). ( B , C ) MMP2/MMP9 gelatinolytic activities (as assayed by gelatin zymography) in MDA-MB-231 and shERβ MDA-MB-231 cells, before and after treatments (AG1478, E2, mix and 24 h). Each bar represents mean ± SD values from triplicate samples. Statistically significant differences are indicated accordingly: * p

    Article Snippet: Morphology of Breast Cancer Cells Cultured on Millipore Filter Covered by Type I Collagen (3000 µg/mL)Untreated (control) and treated (E2, AG1478 and mix) MDA-MB-231 and shERβ MDA-MB-231 cells cultivated on a Millipore filter covered by type I collagen 3000 µg/mL were observed at SEM.

    Techniques: Inhibition, Expressing, Activity Assay, Multiple Displacement Amplification, Quantitative RT-PCR, Zymography

    ANGPTL4 enhances breast cancer cell metastasis to the lungs. ( A–C ) pBabe and pAngptl4 subclones of MDA-MB-231 DKD cells were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pBabe ( C ). *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: ANGPTL4 enhances breast cancer cell metastasis to the lungs. ( A–C ) pBabe and pAngptl4 subclones of MDA-MB-231 DKD cells were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pBabe ( C ). *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Injection, Mouse Assay, Staining, Expressing, Microscopy, Real-time Polymerase Chain Reaction

    L1CAM promotes breast cancer cell metastasis to the lungs. ( A–C ) MDA-MB-231 DKD cells stably transfected with pcDNA3 or pL1CAM were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pcDNA3 ( C ). *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: L1CAM promotes breast cancer cell metastasis to the lungs. ( A–C ) MDA-MB-231 DKD cells stably transfected with pcDNA3 or pL1CAM were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pcDNA3 ( C ). *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Stable Transfection, Transfection, Injection, Mouse Assay, Staining, Expressing, Microscopy, Real-time Polymerase Chain Reaction

    HIF-1 modulates interaction of breast cancer cells with endothelium. ( A–C ) Human umbilical vein endothelial cell (HUVEC) monolayers in a modified Boyden chamber were exposed to CM from ( A–B ) MDA-MB-231 subclones (EV or DKD) or ( C ) parental MDA-MB-231 cells (treated with vehicle or 100 nM digoxin) that were cultured at 20% or 1% O 2 for 48 h. CMFDA-labeled naive MDA-MB-231 cells were then added and the number of cells that invaded through the HUVEC monolayer was counted under fluorescent microscopy (mean ± SD; n = 9; *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: HIF-1 modulates interaction of breast cancer cells with endothelium. ( A–C ) Human umbilical vein endothelial cell (HUVEC) monolayers in a modified Boyden chamber were exposed to CM from ( A–B ) MDA-MB-231 subclones (EV or DKD) or ( C ) parental MDA-MB-231 cells (treated with vehicle or 100 nM digoxin) that were cultured at 20% or 1% O 2 for 48 h. CMFDA-labeled naive MDA-MB-231 cells were then added and the number of cells that invaded through the HUVEC monolayer was counted under fluorescent microscopy (mean ± SD; n = 9; *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Modification, Multiple Displacement Amplification, Cell Culture, Labeling, Microscopy

    HIF-1 promotes the extravasation of breast cancer cells. ( A–C ) GFP-expressing MDA-MB-231 cells (EV or DKD) were cultured at 20% or 1% O 2 for 48 h and then injected into the tail vein of SCID mice. After 1 week, the mice were sacrificed and lung sections were stained with fluorescently-labeled isolectin B4 ( A ). Extravasated GFP-expressing cancer cells ( yellow ) in isolectin B4-stained lung tissues ( red ) were counted ( B ). To quantify the lung breast cancer (BrCa) cell burden, total lung DNA was analyzed by qPCR with human-specific primers and the results were normalized to EV-20% ( C ). In B and C , mean ± SEM are shown ( n = 5); *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: HIF-1 promotes the extravasation of breast cancer cells. ( A–C ) GFP-expressing MDA-MB-231 cells (EV or DKD) were cultured at 20% or 1% O 2 for 48 h and then injected into the tail vein of SCID mice. After 1 week, the mice were sacrificed and lung sections were stained with fluorescently-labeled isolectin B4 ( A ). Extravasated GFP-expressing cancer cells ( yellow ) in isolectin B4-stained lung tissues ( red ) were counted ( B ). To quantify the lung breast cancer (BrCa) cell burden, total lung DNA was analyzed by qPCR with human-specific primers and the results were normalized to EV-20% ( C ). In B and C , mean ± SEM are shown ( n = 5); *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Expressing, Multiple Displacement Amplification, Cell Culture, Injection, Mouse Assay, Staining, Labeling, Real-time Polymerase Chain Reaction

    ANGPTL4 expression is regulated by HIF-1 and inhibits EC-EC interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h. ANGPTL4 mRNA expression was determined by reverse-transcription (RT) qPCR, relative to EV-20% (mean ± SD, n = 3); *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: ANGPTL4 expression is regulated by HIF-1 and inhibits EC-EC interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h. ANGPTL4 mRNA expression was determined by reverse-transcription (RT) qPCR, relative to EV-20% (mean ± SD, n = 3); *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Expressing, Multiple Displacement Amplification, Cell Culture, Quantitative RT-PCR

    L1CAM is regulated by HIF-1 and stimulates EC-cancer cell interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h and L1CAM mRNA was analyzed by RT-qPCR (mean ± SD, n = 3). *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: L1CAM is regulated by HIF-1 and stimulates EC-cancer cell interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h and L1CAM mRNA was analyzed by RT-qPCR (mean ± SD, n = 3). *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Cell Culture, Quantitative RT-PCR

    HIF-1 promotes metastasis of breast cancer to the lungs. ( A ) MDA-MB-231 cells were stably transfected with a lentiviral vector encoding a short hairpin RNA directed against HIF-1α (sh1α), HIF-2α (sh2α), or both (DKD) or with empty vector (EV). Cells were exposed to 20% or 1% O 2 for 4 h and immunoblot assays were performed using whole cell lysates and antibodies against HIF-1α, HIF-2α, or β-actin. ( B–F ) Each of the MDA-MB-231 subclones was implanted into the mammary fat pad (MFP) of SCID mice ( n = 5 mice per subclone). Primary tumor volume ( B ) was determined from day 9 to day 24 (mean ± SEM; *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: HIF-1 promotes metastasis of breast cancer to the lungs. ( A ) MDA-MB-231 cells were stably transfected with a lentiviral vector encoding a short hairpin RNA directed against HIF-1α (sh1α), HIF-2α (sh2α), or both (DKD) or with empty vector (EV). Cells were exposed to 20% or 1% O 2 for 4 h and immunoblot assays were performed using whole cell lysates and antibodies against HIF-1α, HIF-2α, or β-actin. ( B–F ) Each of the MDA-MB-231 subclones was implanted into the mammary fat pad (MFP) of SCID mice ( n = 5 mice per subclone). Primary tumor volume ( B ) was determined from day 9 to day 24 (mean ± SEM; *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Stable Transfection, Transfection, Plasmid Preparation, shRNA, Mouse Assay

    Bland–Altman plot describing the agreement between tumor genome fraction (TGF) measures obtained with ichorCNA ( A ), control-FREEC ( B ) and the real tumor fractions (TFs). Differences between triplicate TGF measures and real TFs are separately reported for MDA-MB-361 (red triangles) and MDA-MB-453 (green squares) as a function of the measures’ averages. Linear fit for each cell line is reported with intervals of confidence. Dashed lines from top to bottom represent the mean of difference plus two standard deviations, mean of difference and mean of difference minus two standard deviations.

    Journal: Cancers

    Article Title: Detection of Genomically Aberrant Cells within Circulating Tumor Microemboli (CTMs) Isolated from Early-Stage Breast Cancer Patients

    doi: 10.3390/cancers13061409

    Figure Lengend Snippet: Bland–Altman plot describing the agreement between tumor genome fraction (TGF) measures obtained with ichorCNA ( A ), control-FREEC ( B ) and the real tumor fractions (TFs). Differences between triplicate TGF measures and real TFs are separately reported for MDA-MB-361 (red triangles) and MDA-MB-453 (green squares) as a function of the measures’ averages. Linear fit for each cell line is reported with intervals of confidence. Dashed lines from top to bottom represent the mean of difference plus two standard deviations, mean of difference and mean of difference minus two standard deviations.

    Article Snippet: Cell Lines and Generation of Cell Mixtures Breast cancer cell lines MDA-MB-361 (passage number 22) and MDA-MB-453 (passage number 13) [ ] were obtained from the American Type Culture Collection (ATCC).

    Techniques: Multiple Displacement Amplification

    Comparison between ichorCNA and control-FREEC. Copy number alteration (CNA) profiles obtained with low-pass whole genome sequencing (lpWGS) from samples formed by 100% MDA-MB-361 cells (top row), 100% peripheral blood lymphocytes (PBL, bottom row), and artificially-generated mixed samples containing 60% and 20% MDA-MB-361 cells mixed with PBL (second and third row from the top, respectively). CNA profiles reported on the left-hand side were obtained with control-FREEC algorithm; CNA profiles on the right hand side were obtained with ichorCNA. In the case of control-FREEC profiles, different colors refer to loss, gain or their absence (normal) in each genomic region; in the case of ichorCNA, color codes refer to 1 copy, 2 copies, 3 copies, more than 4 copies for each single genomic region. Color codes are reported at the bottom of the figure.

    Journal: Cancers

    Article Title: Detection of Genomically Aberrant Cells within Circulating Tumor Microemboli (CTMs) Isolated from Early-Stage Breast Cancer Patients

    doi: 10.3390/cancers13061409

    Figure Lengend Snippet: Comparison between ichorCNA and control-FREEC. Copy number alteration (CNA) profiles obtained with low-pass whole genome sequencing (lpWGS) from samples formed by 100% MDA-MB-361 cells (top row), 100% peripheral blood lymphocytes (PBL, bottom row), and artificially-generated mixed samples containing 60% and 20% MDA-MB-361 cells mixed with PBL (second and third row from the top, respectively). CNA profiles reported on the left-hand side were obtained with control-FREEC algorithm; CNA profiles on the right hand side were obtained with ichorCNA. In the case of control-FREEC profiles, different colors refer to loss, gain or their absence (normal) in each genomic region; in the case of ichorCNA, color codes refer to 1 copy, 2 copies, 3 copies, more than 4 copies for each single genomic region. Color codes are reported at the bottom of the figure.

    Article Snippet: Cell Lines and Generation of Cell Mixtures Breast cancer cell lines MDA-MB-361 (passage number 22) and MDA-MB-453 (passage number 13) [ ] were obtained from the American Type Culture Collection (ATCC).

    Techniques: Sequencing, Multiple Displacement Amplification, Generated