Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs
Figure Lengend Snippet: L1CAM promotes breast cancer cell metastasis to the lungs. ( A–C ) MDA-MB-231 DKD cells stably transfected with pcDNA3 or pL1CAM were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pcDNA3 ( C ). *, P
Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).
Techniques: Multiple Displacement Amplification, Stable Transfection, Transfection, Injection, Mouse Assay, Staining, Expressing, Microscopy, Real-time Polymerase Chain Reaction