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    Qiagen mda reaction mix
    Mda Reaction Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda reaction mix/product/Qiagen
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    mda reaction mix - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    85
    Becton Dickinson mda mb 231 cells mixed
    ANGPTL4 enhances breast cancer cell metastasis to the lungs. ( A–C ) pBabe and pAngptl4 subclones of <t>MDA-MB-231</t> DKD cells were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pBabe ( C ). *, P
    Mda Mb 231 Cells Mixed, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 231 cells mixed/product/Becton Dickinson
    Average 85 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    mda mb 231 cells mixed - by Bioz Stars, 2020-12
    85/100 stars
      Buy from Supplier

    Image Search Results


    ANGPTL4 enhances breast cancer cell metastasis to the lungs. ( A–C ) pBabe and pAngptl4 subclones of MDA-MB-231 DKD cells were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pBabe ( C ). *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: ANGPTL4 enhances breast cancer cell metastasis to the lungs. ( A–C ) pBabe and pAngptl4 subclones of MDA-MB-231 DKD cells were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pBabe ( C ). *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Injection, Mouse Assay, Staining, Expressing, Microscopy, Real-time Polymerase Chain Reaction

    L1CAM promotes breast cancer cell metastasis to the lungs. ( A–C ) MDA-MB-231 DKD cells stably transfected with pcDNA3 or pL1CAM were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pcDNA3 ( C ). *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: L1CAM promotes breast cancer cell metastasis to the lungs. ( A–C ) MDA-MB-231 DKD cells stably transfected with pcDNA3 or pL1CAM were injected into the tail vein of SCID mice. After 1 week, lung tissues were harvested, sections stained with isolectin B4, and GFP-expressing cancer cells ( A ) were counted under fluorescent microscopy ( B ). To determine the lung BrCa burden, lung DNA was analyzed by qPCR with GFP primers and the results (mean ± SEM, n = 5) were normalized to pcDNA3 ( C ). *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Stable Transfection, Transfection, Injection, Mouse Assay, Staining, Expressing, Microscopy, Real-time Polymerase Chain Reaction

    HIF-1 modulates interaction of breast cancer cells with endothelium. ( A–C ) Human umbilical vein endothelial cell (HUVEC) monolayers in a modified Boyden chamber were exposed to CM from ( A–B ) MDA-MB-231 subclones (EV or DKD) or ( C ) parental MDA-MB-231 cells (treated with vehicle or 100 nM digoxin) that were cultured at 20% or 1% O 2 for 48 h. CMFDA-labeled naive MDA-MB-231 cells were then added and the number of cells that invaded through the HUVEC monolayer was counted under fluorescent microscopy (mean ± SD; n = 9; *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: HIF-1 modulates interaction of breast cancer cells with endothelium. ( A–C ) Human umbilical vein endothelial cell (HUVEC) monolayers in a modified Boyden chamber were exposed to CM from ( A–B ) MDA-MB-231 subclones (EV or DKD) or ( C ) parental MDA-MB-231 cells (treated with vehicle or 100 nM digoxin) that were cultured at 20% or 1% O 2 for 48 h. CMFDA-labeled naive MDA-MB-231 cells were then added and the number of cells that invaded through the HUVEC monolayer was counted under fluorescent microscopy (mean ± SD; n = 9; *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Modification, Multiple Displacement Amplification, Cell Culture, Labeling, Microscopy

    HIF-1 promotes the extravasation of breast cancer cells. ( A–C ) GFP-expressing MDA-MB-231 cells (EV or DKD) were cultured at 20% or 1% O 2 for 48 h and then injected into the tail vein of SCID mice. After 1 week, the mice were sacrificed and lung sections were stained with fluorescently-labeled isolectin B4 ( A ). Extravasated GFP-expressing cancer cells ( yellow ) in isolectin B4-stained lung tissues ( red ) were counted ( B ). To quantify the lung breast cancer (BrCa) cell burden, total lung DNA was analyzed by qPCR with human-specific primers and the results were normalized to EV-20% ( C ). In B and C , mean ± SEM are shown ( n = 5); *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: HIF-1 promotes the extravasation of breast cancer cells. ( A–C ) GFP-expressing MDA-MB-231 cells (EV or DKD) were cultured at 20% or 1% O 2 for 48 h and then injected into the tail vein of SCID mice. After 1 week, the mice were sacrificed and lung sections were stained with fluorescently-labeled isolectin B4 ( A ). Extravasated GFP-expressing cancer cells ( yellow ) in isolectin B4-stained lung tissues ( red ) were counted ( B ). To quantify the lung breast cancer (BrCa) cell burden, total lung DNA was analyzed by qPCR with human-specific primers and the results were normalized to EV-20% ( C ). In B and C , mean ± SEM are shown ( n = 5); *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Expressing, Multiple Displacement Amplification, Cell Culture, Injection, Mouse Assay, Staining, Labeling, Real-time Polymerase Chain Reaction

    ANGPTL4 expression is regulated by HIF-1 and inhibits EC-EC interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h. ANGPTL4 mRNA expression was determined by reverse-transcription (RT) qPCR, relative to EV-20% (mean ± SD, n = 3); *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: ANGPTL4 expression is regulated by HIF-1 and inhibits EC-EC interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h. ANGPTL4 mRNA expression was determined by reverse-transcription (RT) qPCR, relative to EV-20% (mean ± SD, n = 3); *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Expressing, Multiple Displacement Amplification, Cell Culture, Quantitative RT-PCR

    L1CAM is regulated by HIF-1 and stimulates EC-cancer cell interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h and L1CAM mRNA was analyzed by RT-qPCR (mean ± SD, n = 3). *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: L1CAM is regulated by HIF-1 and stimulates EC-cancer cell interaction. ( A ) MDA-MB-231 subclones were cultured at 20% or 1% O 2 for 24 h and L1CAM mRNA was analyzed by RT-qPCR (mean ± SD, n = 3). *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Cell Culture, Quantitative RT-PCR

    HIF-1 promotes metastasis of breast cancer to the lungs. ( A ) MDA-MB-231 cells were stably transfected with a lentiviral vector encoding a short hairpin RNA directed against HIF-1α (sh1α), HIF-2α (sh2α), or both (DKD) or with empty vector (EV). Cells were exposed to 20% or 1% O 2 for 4 h and immunoblot assays were performed using whole cell lysates and antibodies against HIF-1α, HIF-2α, or β-actin. ( B–F ) Each of the MDA-MB-231 subclones was implanted into the mammary fat pad (MFP) of SCID mice ( n = 5 mice per subclone). Primary tumor volume ( B ) was determined from day 9 to day 24 (mean ± SEM; *, P

    Journal: Oncogene

    Article Title: HIF-1-dependent Expression of Angiopoietin-like 4 and L1CAM Mediates Vascular Metastasis of Hypoxic Breast Cancer Cells to the Lungs

    doi: 10.1038/onc.2011.365

    Figure Lengend Snippet: HIF-1 promotes metastasis of breast cancer to the lungs. ( A ) MDA-MB-231 cells were stably transfected with a lentiviral vector encoding a short hairpin RNA directed against HIF-1α (sh1α), HIF-2α (sh2α), or both (DKD) or with empty vector (EV). Cells were exposed to 20% or 1% O 2 for 4 h and immunoblot assays were performed using whole cell lysates and antibodies against HIF-1α, HIF-2α, or β-actin. ( B–F ) Each of the MDA-MB-231 subclones was implanted into the mammary fat pad (MFP) of SCID mice ( n = 5 mice per subclone). Primary tumor volume ( B ) was determined from day 9 to day 24 (mean ± SEM; *, P

    Article Snippet: For xenograft assays, 4–6 week-old athymic nude mice (NCI) received a flank injection of 2×106 MDA-MB-231 cells mixed 1:1 with Matrigel (BD Biosciences).

    Techniques: Multiple Displacement Amplification, Stable Transfection, Transfection, Plasmid Preparation, shRNA, Mouse Assay