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    New England Biolabs mcherry ptp1b d181a
    Identification of <t>PTP1B</t> (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant <t>D181A</t> and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without RHBDL4mycFLAG. The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.
    Mcherry Ptp1b D181a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcherry ptp1b d181a/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
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    mcherry ptp1b d181a - by Bioz Stars, 2022-09
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    Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without RHBDL4mycFLAG. The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.

    Journal: The Journal of Biological Chemistry

    Article Title: Spatial proteomics reveal that the protein phosphatase PTP1B interacts with and may modify tyrosine phosphorylation of the rhomboid protease RHBDL4

    doi: 10.1074/jbc.RA118.007074

    Figure Lengend Snippet: Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without RHBDL4mycFLAG. The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.

    Article Snippet: The PCR product was cloned into SgfI/XhoI–excised RC210708 plasmid, by Gibson assembly (NEB). mCherry-PTP1B D181A was obtained from Addgene (40270). mCherry-PTP1B WT was generated by reverting the Ala181 codon back to aspartate, whereas super-trapping mutant D181A/Y46F was generated by site-directed mutagenesis of Tyr46 to Phe on the D181A mutant (Addgene, 40270).

    Techniques: Mutagenesis, Immunoprecipitation, Expressing