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    TaKaRa mcherry gene
    Codon usage optimization of the amCyan and <t>mCherry</t> genes. ( a ) The fluorescing colonies were photographed under UV excitation. S . aureus strain MW2 containing pKAT (control), pFK62 (AmCyan), pFK64 (AmCyan(S.a)), pFK63 (mCherry), and pFK65 (mCherry(S.a)) were grown on TSB agar plates containing chloramphenicol. ( b ) SDS-PAGE analysis showed the relative quantities of AmCyan and mCherry in the whole cell lysates. Arrows indicate the position of AmCyan and mCherry in gel. ( c ) Western blot analysis of AmCyan or mCherry in the whole cell lysates. The Western blot gels were cropped and the full-length image are included in Supplemental Fig. 5(d) . The comparison of fluorescent intensities of pKAT (control), pFK62 (AmCyan), and pFK64 (AmCyan(S.a)) in S . aureus MW2. The fluorescent intensities at 489 nm were measured with a microplate reader, λ ex = 458 nm. The data represent mean values ± standard deviation. ( e ) The comparison of fluorescent intensities of pKAT (control), pFK63 (mCherry), and pFK65 (mChaerry(S.a)) in S . aureus MW2. The fluorescent intensities at 610 nm were measured with a microplate reader, λ ex = 586 nm. The data represent mean values ± standard deviation.
    Mcherry Gene, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Addgene inc mcherry gene
    Identification of the central crisis pathway. A , B The mouse connectivity data for AAV-EYFP injection in the NST ( A ) and the Sp5 ( B ) derived from the Allen Mouse Brain Connectivity Atlas (2011) (sagittal images in the left and enlarged coronal images in the upper columns in the right panels), and representative images of in situ hybridization of c-fos mRNA in response to 4E2MT (enlarged coronal images in the lower columns in the right panels). C Mean survival time in 4% oxygen in response to IP administration of the odorants indicated ( n = 6 for saline, n = 6 for 2MO, n = 6 for TO, n = 12 for 2MT, and n = 8 for 4E2MT). D – G Quantification of c-fos mRNA expression in response to IP administration of the indicated odorants in the PAG ( D ), SC ( E ), MDRN ( F ), and PBN ( G ) ( n = 6 for saline, 2MO, 2MT, and 4E2MT, and n = 8 for TO). Mean expression in saline conditions was set at 100%. H Experimental design for chemogenetic activation of the NST-PBN pathway. I , J Representative fluorescent ( I ) and bright ( J ) images of hM3Dq-fused <t>mCherry</t> expression in the NST for AAV-FLEX-hM3Dq-mCherry injected animal. K – M Temporal analyses of cutaneous temperature ( K ), freezing behavior ( L ), and oxygen consumption ( M ) after CNO administration are shown for AAV-FLEX-hM3Dq-infected (red) and control (gray) mice ( K ; n = 5 for control and n = 7 for hM3Dq, L ; n = 5 for control and n = 6 for hM3Dq, M ; n = 8 for control and n = 6 for hM3Dq). Statistical significance was assessed for 20 min after CNO administration. Data are shown as mean ± SEM. Student’s t test followed by Bonferroni correction or two-way ANOVA followed by Sidak’s multiple comparisons was used to assess significance. * P
    Mcherry Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcherry gene/product/Addgene inc
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mcherry gene - by Bioz Stars, 2022-09
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    99
    Addgene inc mcherry gene aav5 hsyn1 dio hm3d gq mcherry
    Identification of the central crisis pathway. A , B The mouse connectivity data for AAV-EYFP injection in the NST ( A ) and the Sp5 ( B ) derived from the Allen Mouse Brain Connectivity Atlas (2011) (sagittal images in the left and enlarged coronal images in the upper columns in the right panels), and representative images of in situ hybridization of c-fos mRNA in response to 4E2MT (enlarged coronal images in the lower columns in the right panels). C Mean survival time in 4% oxygen in response to IP administration of the odorants indicated ( n = 6 for saline, n = 6 for 2MO, n = 6 for TO, n = 12 for 2MT, and n = 8 for 4E2MT). D – G Quantification of c-fos mRNA expression in response to IP administration of the indicated odorants in the PAG ( D ), SC ( E ), MDRN ( F ), and PBN ( G ) ( n = 6 for saline, 2MO, 2MT, and 4E2MT, and n = 8 for TO). Mean expression in saline conditions was set at 100%. H Experimental design for chemogenetic activation of the NST-PBN pathway. I , J Representative fluorescent ( I ) and bright ( J ) images of hM3Dq-fused <t>mCherry</t> expression in the NST for AAV-FLEX-hM3Dq-mCherry injected animal. K – M Temporal analyses of cutaneous temperature ( K ), freezing behavior ( L ), and oxygen consumption ( M ) after CNO administration are shown for AAV-FLEX-hM3Dq-infected (red) and control (gray) mice ( K ; n = 5 for control and n = 7 for hM3Dq, L ; n = 5 for control and n = 6 for hM3Dq, M ; n = 8 for control and n = 6 for hM3Dq). Statistical significance was assessed for 20 min after CNO administration. Data are shown as mean ± SEM. Student’s t test followed by Bonferroni correction or two-way ANOVA followed by Sidak’s multiple comparisons was used to assess significance. * P
    Mcherry Gene Aav5 Hsyn1 Dio Hm3d Gq Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcherry gene aav5 hsyn1 dio hm3d gq mcherry/product/Addgene inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mcherry gene aav5 hsyn1 dio hm3d gq mcherry - by Bioz Stars, 2022-09
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    Image Search Results


    Codon usage optimization of the amCyan and mCherry genes. ( a ) The fluorescing colonies were photographed under UV excitation. S . aureus strain MW2 containing pKAT (control), pFK62 (AmCyan), pFK64 (AmCyan(S.a)), pFK63 (mCherry), and pFK65 (mCherry(S.a)) were grown on TSB agar plates containing chloramphenicol. ( b ) SDS-PAGE analysis showed the relative quantities of AmCyan and mCherry in the whole cell lysates. Arrows indicate the position of AmCyan and mCherry in gel. ( c ) Western blot analysis of AmCyan or mCherry in the whole cell lysates. The Western blot gels were cropped and the full-length image are included in Supplemental Fig. 5(d) . The comparison of fluorescent intensities of pKAT (control), pFK62 (AmCyan), and pFK64 (AmCyan(S.a)) in S . aureus MW2. The fluorescent intensities at 489 nm were measured with a microplate reader, λ ex = 458 nm. The data represent mean values ± standard deviation. ( e ) The comparison of fluorescent intensities of pKAT (control), pFK63 (mCherry), and pFK65 (mChaerry(S.a)) in S . aureus MW2. The fluorescent intensities at 610 nm were measured with a microplate reader, λ ex = 586 nm. The data represent mean values ± standard deviation.

    Journal: Scientific Reports

    Article Title: The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus

    doi: 10.1038/s41598-017-02930-7

    Figure Lengend Snippet: Codon usage optimization of the amCyan and mCherry genes. ( a ) The fluorescing colonies were photographed under UV excitation. S . aureus strain MW2 containing pKAT (control), pFK62 (AmCyan), pFK64 (AmCyan(S.a)), pFK63 (mCherry), and pFK65 (mCherry(S.a)) were grown on TSB agar plates containing chloramphenicol. ( b ) SDS-PAGE analysis showed the relative quantities of AmCyan and mCherry in the whole cell lysates. Arrows indicate the position of AmCyan and mCherry in gel. ( c ) Western blot analysis of AmCyan or mCherry in the whole cell lysates. The Western blot gels were cropped and the full-length image are included in Supplemental Fig. 5(d) . The comparison of fluorescent intensities of pKAT (control), pFK62 (AmCyan), and pFK64 (AmCyan(S.a)) in S . aureus MW2. The fluorescent intensities at 489 nm were measured with a microplate reader, λ ex = 458 nm. The data represent mean values ± standard deviation. ( e ) The comparison of fluorescent intensities of pKAT (control), pFK63 (mCherry), and pFK65 (mChaerry(S.a)) in S . aureus MW2. The fluorescent intensities at 610 nm were measured with a microplate reader, λ ex = 586 nm. The data represent mean values ± standard deviation.

    Article Snippet: In the first PCR, the hybrid blaZ /sodp (Con) promoter region, amCyan gene, and mCherry gene were amplified from pFK53, pAmCyan (Clontech, TaKaRa Bio Inc., Japan), and pmCherry (Clontech, TaKaRa Bio Inc., Japan) with the following primer sets (AmCyan: blaZp-F and blaZPR-Cyan and blaZP-CyanF and pUC-RH; mCherry: blaZp-F and blaZPR-Cherry and blaZP-mCherryF and pUC-RH), respectively.

    Techniques: SDS Page, Western Blot, Standard Deviation

    Confocal laser microscopic analysis in co-culture systems. ( a ) Co-culture of three clinically isolated S . aureus strains. S . aureus strain N315 containing pFK60 (Cerulean), strain TY34 containing pFK56 (Citrine), and strain MW2 containing pFK65 (mCherry(S.a)) were co-cultured in BHI broth. Panels show (1) Cerulean (N315), (2) Citrine (TY34), (3) mCherry(S.a) (MW2), (4) overlay of Cerulean, Citrine, and mCherry images, (5) DIC, (6) overlay of Cerulean, Citrine, mCherry, and DIC images. ( b ) Co-culture of S . aureus with E . coli . S . aureus N315 containing pFK65 (mCherry(S.a)) and E . coli DH5α containing pFK55 (EGFP) were co-cultured in BHI broth. Panels show (1) DAPI, (2) EGFP, (3) mCherry(S.a), (4) DIC, (5) overlay of DAPI and DIC images, and (6) overlay of EGFP, mCherry(S.a), and DIC images.

    Journal: Scientific Reports

    Article Title: The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus

    doi: 10.1038/s41598-017-02930-7

    Figure Lengend Snippet: Confocal laser microscopic analysis in co-culture systems. ( a ) Co-culture of three clinically isolated S . aureus strains. S . aureus strain N315 containing pFK60 (Cerulean), strain TY34 containing pFK56 (Citrine), and strain MW2 containing pFK65 (mCherry(S.a)) were co-cultured in BHI broth. Panels show (1) Cerulean (N315), (2) Citrine (TY34), (3) mCherry(S.a) (MW2), (4) overlay of Cerulean, Citrine, and mCherry images, (5) DIC, (6) overlay of Cerulean, Citrine, mCherry, and DIC images. ( b ) Co-culture of S . aureus with E . coli . S . aureus N315 containing pFK65 (mCherry(S.a)) and E . coli DH5α containing pFK55 (EGFP) were co-cultured in BHI broth. Panels show (1) DAPI, (2) EGFP, (3) mCherry(S.a), (4) DIC, (5) overlay of DAPI and DIC images, and (6) overlay of EGFP, mCherry(S.a), and DIC images.

    Article Snippet: In the first PCR, the hybrid blaZ /sodp (Con) promoter region, amCyan gene, and mCherry gene were amplified from pFK53, pAmCyan (Clontech, TaKaRa Bio Inc., Japan), and pmCherry (Clontech, TaKaRa Bio Inc., Japan) with the following primer sets (AmCyan: blaZp-F and blaZPR-Cyan and blaZP-CyanF and pUC-RH; mCherry: blaZp-F and blaZPR-Cherry and blaZP-mCherryF and pUC-RH), respectively.

    Techniques: Co-Culture Assay, Isolation, Cell Culture

    Identification of the central crisis pathway. A , B The mouse connectivity data for AAV-EYFP injection in the NST ( A ) and the Sp5 ( B ) derived from the Allen Mouse Brain Connectivity Atlas (2011) (sagittal images in the left and enlarged coronal images in the upper columns in the right panels), and representative images of in situ hybridization of c-fos mRNA in response to 4E2MT (enlarged coronal images in the lower columns in the right panels). C Mean survival time in 4% oxygen in response to IP administration of the odorants indicated ( n = 6 for saline, n = 6 for 2MO, n = 6 for TO, n = 12 for 2MT, and n = 8 for 4E2MT). D – G Quantification of c-fos mRNA expression in response to IP administration of the indicated odorants in the PAG ( D ), SC ( E ), MDRN ( F ), and PBN ( G ) ( n = 6 for saline, 2MO, 2MT, and 4E2MT, and n = 8 for TO). Mean expression in saline conditions was set at 100%. H Experimental design for chemogenetic activation of the NST-PBN pathway. I , J Representative fluorescent ( I ) and bright ( J ) images of hM3Dq-fused mCherry expression in the NST for AAV-FLEX-hM3Dq-mCherry injected animal. K – M Temporal analyses of cutaneous temperature ( K ), freezing behavior ( L ), and oxygen consumption ( M ) after CNO administration are shown for AAV-FLEX-hM3Dq-infected (red) and control (gray) mice ( K ; n = 5 for control and n = 7 for hM3Dq, L ; n = 5 for control and n = 6 for hM3Dq, M ; n = 8 for control and n = 6 for hM3Dq). Statistical significance was assessed for 20 min after CNO administration. Data are shown as mean ± SEM. Student’s t test followed by Bonferroni correction or two-way ANOVA followed by Sidak’s multiple comparisons was used to assess significance. * P

    Journal: Communications Biology

    Article Title: Artificial hibernation/life-protective state induced by thiazoline-related innate fear odors

    doi: 10.1038/s42003-020-01629-2

    Figure Lengend Snippet: Identification of the central crisis pathway. A , B The mouse connectivity data for AAV-EYFP injection in the NST ( A ) and the Sp5 ( B ) derived from the Allen Mouse Brain Connectivity Atlas (2011) (sagittal images in the left and enlarged coronal images in the upper columns in the right panels), and representative images of in situ hybridization of c-fos mRNA in response to 4E2MT (enlarged coronal images in the lower columns in the right panels). C Mean survival time in 4% oxygen in response to IP administration of the odorants indicated ( n = 6 for saline, n = 6 for 2MO, n = 6 for TO, n = 12 for 2MT, and n = 8 for 4E2MT). D – G Quantification of c-fos mRNA expression in response to IP administration of the indicated odorants in the PAG ( D ), SC ( E ), MDRN ( F ), and PBN ( G ) ( n = 6 for saline, 2MO, 2MT, and 4E2MT, and n = 8 for TO). Mean expression in saline conditions was set at 100%. H Experimental design for chemogenetic activation of the NST-PBN pathway. I , J Representative fluorescent ( I ) and bright ( J ) images of hM3Dq-fused mCherry expression in the NST for AAV-FLEX-hM3Dq-mCherry injected animal. K – M Temporal analyses of cutaneous temperature ( K ), freezing behavior ( L ), and oxygen consumption ( M ) after CNO administration are shown for AAV-FLEX-hM3Dq-infected (red) and control (gray) mice ( K ; n = 5 for control and n = 7 for hM3Dq, L ; n = 5 for control and n = 6 for hM3Dq, M ; n = 8 for control and n = 6 for hM3Dq). Statistical significance was assessed for 20 min after CNO administration. Data are shown as mean ± SEM. Student’s t test followed by Bonferroni correction or two-way ANOVA followed by Sidak’s multiple comparisons was used to assess significance. * P

    Article Snippet: An AAV virus carrying a double-floxed inverted hM3D(Gq) fused with mCherry gene (AAV5-hSyn1-DIO-hM3D(Gq)-mCherry , Addgene), double-floxed inverted hM4Di(Gi) fused with mCherry gene (AAV5-hSyn1-DIO-hM4Di(Gi)-mCherry , Addgene) or mCherry gene (AAV5-hSyn1-DIO-mCherry , Addgene) was infused into the NST with 80 nL of virus solution at the following coordinates: anterior–posterior (AP), 0.4 mm caudal from the caudal end of cerebellum; left–right (LR), ±0.2 mm; dorsoventral (DV), 0.6 mm from the surface.

    Techniques: Injection, Derivative Assay, In Situ Hybridization, Expressing, Activation Assay, Infection, Mouse Assay