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  • 99
    New England Biolabs mboi
    Digestion pattern in the presence of 0.1 µg hybrid 3:4 ( A ) or 2:4 ( B ) and 1 or 10 U <t>MboI</t> or <t>DpnII</t> (indicated on top of phosphor image). The reaction temperature was 37°C and the reaction time 10 (lane 1), 30 (lane 2) or 120 min (lane 3).
    Mboi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mboi/product/New England Biolabs
    Average 99 stars, based on 766 article reviews
    Price from $9.99 to $1999.99
    mboi - by Bioz Stars, 2020-08
    99/100 stars
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    92
    Thermo Fisher 4 cutter fastdigest mboi
    Digestion pattern in the presence of 0.1 µg hybrid 3:4 ( A ) or 2:4 ( B ) and 1 or 10 U <t>MboI</t> or <t>DpnII</t> (indicated on top of phosphor image). The reaction temperature was 37°C and the reaction time 10 (lane 1), 30 (lane 2) or 120 min (lane 3).
    4 Cutter Fastdigest Mboi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 cutter fastdigest mboi/product/Thermo Fisher
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    4 cutter fastdigest mboi - by Bioz Stars, 2020-08
    92/100 stars
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    88
    Promega mboi
    Effects of host plant, Aquilaria crassna (agarwood) and Tectona grandis (teak), and source of samples (root and soil) on mean number of terminal restriction fragments (TRFs) per sample using three restriction enzymes <t>MboI</t> (open bars), <t>HinfI</t> (hatched bars) and Hsp92II (cross-hatched bars). Values are mean ± SEM (n = 4 for teak and n = 3 for agarwood).
    Mboi, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mboi/product/Promega
    Average 88 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    mboi - by Bioz Stars, 2020-08
    88/100 stars
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    99
    New England Biolabs mboi endonuclease
    Effects of host plant, Aquilaria crassna (agarwood) and Tectona grandis (teak), and source of samples (root and soil) on mean number of terminal restriction fragments (TRFs) per sample using three restriction enzymes <t>MboI</t> (open bars), <t>HinfI</t> (hatched bars) and Hsp92II (cross-hatched bars). Values are mean ± SEM (n = 4 for teak and n = 3 for agarwood).
    Mboi Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mboi endonuclease/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mboi endonuclease - by Bioz Stars, 2020-08
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    mboi  (Roche)
    91
    Roche mboi
    Effects of host plant, Aquilaria crassna (agarwood) and Tectona grandis (teak), and source of samples (root and soil) on mean number of terminal restriction fragments (TRFs) per sample using three restriction enzymes <t>MboI</t> (open bars), <t>HinfI</t> (hatched bars) and Hsp92II (cross-hatched bars). Values are mean ± SEM (n = 4 for teak and n = 3 for agarwood).
    Mboi, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mboi/product/Roche
    Average 91 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
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    91
    Stratagene mboi
    Effects of host plant, Aquilaria crassna (agarwood) and Tectona grandis (teak), and source of samples (root and soil) on mean number of terminal restriction fragments (TRFs) per sample using three restriction enzymes <t>MboI</t> (open bars), <t>HinfI</t> (hatched bars) and Hsp92II (cross-hatched bars). Values are mean ± SEM (n = 4 for teak and n = 3 for agarwood).
    Mboi, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mboi/product/Stratagene
    Average 91 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    mboi - by Bioz Stars, 2020-08
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    94
    Thermo Fisher mboi
    Identification of methylated recognition sequences in S. acidocaldarius genomic DNA by digestion assays. Restriction enzymes <t>BsuRI</t> (A) and BamHI, DpnI, and <t>MboI</t> (B) were used to highlight the presence/absence of methylated 5′-GGCC-3′ and 5′-GATC-3′ palindromes, respectively, in genomic DNA of S. acidocaldarius . Two types of substrates were digested: genomic DNA (gDNA) and a whole genome amplification of the genomic DNA (WGA). WGA is identical to gDNA in terms of nucleic sequence but it does not contain epigenetic marks (see material and methods). This negative control is only used in (A) . The addition of different restriction enzymes is symbolized by a positive sign “+” while reaction mix without restriction enzyme is represented by a negative sign “–”. Molecular size markers (GeneRuler DNA Ladder, Thermo Scientific) were loaded in both gels (M). Digestion patterns were obtained in 0.8% agarose gel stained with ethidium bromide and visualized under UV light.
    Mboi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa restriction enzyme mboi
    Identification of methylated recognition sequences in S. acidocaldarius genomic DNA by digestion assays. Restriction enzymes <t>BsuRI</t> (A) and BamHI, DpnI, and <t>MboI</t> (B) were used to highlight the presence/absence of methylated 5′-GGCC-3′ and 5′-GATC-3′ palindromes, respectively, in genomic DNA of S. acidocaldarius . Two types of substrates were digested: genomic DNA (gDNA) and a whole genome amplification of the genomic DNA (WGA). WGA is identical to gDNA in terms of nucleic sequence but it does not contain epigenetic marks (see material and methods). This negative control is only used in (A) . The addition of different restriction enzymes is symbolized by a positive sign “+” while reaction mix without restriction enzyme is represented by a negative sign “–”. Molecular size markers (GeneRuler DNA Ladder, Thermo Scientific) were loaded in both gels (M). Digestion patterns were obtained in 0.8% agarose gel stained with ethidium bromide and visualized under UV light.
    Restriction Enzyme Mboi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Digestion pattern in the presence of 0.1 µg hybrid 3:4 ( A ) or 2:4 ( B ) and 1 or 10 U MboI or DpnII (indicated on top of phosphor image). The reaction temperature was 37°C and the reaction time 10 (lane 1), 30 (lane 2) or 120 min (lane 3).

    Journal: Nucleic Acids Research

    Article Title: Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside

    doi: 10.1093/nar/gkg911

    Figure Lengend Snippet: Digestion pattern in the presence of 0.1 µg hybrid 3:4 ( A ) or 2:4 ( B ) and 1 or 10 U MboI or DpnII (indicated on top of phosphor image). The reaction temperature was 37°C and the reaction time 10 (lane 1), 30 (lane 2) or 120 min (lane 3).

    Article Snippet: The final reaction volume contained 0.1 µg hybrid 3:4 or 3:5 or 2:4 or 2:5 and 1 or 10 U MboI or DpnII (all from New England Biolabs).

    Techniques:

    hCtc1(OB) deletion increases ExoI-resistant single-stranded telomere DNA. ( A ) Analysis of single-stranded telomeric DNA from 293T cell lines expressing full length, WT and mutant (double and deletion) hCtc1 with ExoI prior to AluI/MboI digestion. ExoI treated (+) and untreated (−) (ss)TTAGGG signal was detected with 32 P-labeled (CCCTAA) 4 probe under native condition (left) and denaturing conditions (right). The ssTTAGGG signal was normalized to the total TTAGGG signal in the same lane as indicated at the bottom. ( B ) Probing for the presence of single stranded ssCCCTAA signal in the same DNA samples as of panel (A). DNA samples were probed with 32 P-labeled (TTAGGG) 4 under native and denaturing conditions; the results show no CCCTAA signal under native conditions (left) which indicates the absence of single-stranded internal CCCTAA DNA.

    Journal: Nucleic Acids Research

    Article Title: Structural and functional analysis of an OB-fold in human Ctc1 implicated in telomere maintenance and bone marrow syndromes

    doi: 10.1093/nar/gkx1213

    Figure Lengend Snippet: hCtc1(OB) deletion increases ExoI-resistant single-stranded telomere DNA. ( A ) Analysis of single-stranded telomeric DNA from 293T cell lines expressing full length, WT and mutant (double and deletion) hCtc1 with ExoI prior to AluI/MboI digestion. ExoI treated (+) and untreated (−) (ss)TTAGGG signal was detected with 32 P-labeled (CCCTAA) 4 probe under native condition (left) and denaturing conditions (right). The ssTTAGGG signal was normalized to the total TTAGGG signal in the same lane as indicated at the bottom. ( B ) Probing for the presence of single stranded ssCCCTAA signal in the same DNA samples as of panel (A). DNA samples were probed with 32 P-labeled (TTAGGG) 4 under native and denaturing conditions; the results show no CCCTAA signal under native conditions (left) which indicates the absence of single-stranded internal CCCTAA DNA.

    Article Snippet: A total of 15 μg of Exo I treated and untreated genomic DNA was then digested with 15U of AluI and MboI restriction endonucleases (New England Biolabs) overnight at 37°C, and purified by phenol-chloroform extraction.

    Techniques: Expressing, Mutagenesis, Labeling

    Human Ku86-deficient cell lines have shortened telomeres. Genomic DNA was purified from the indicated cell lines, digested to completion with MboI and AluI, and then subjected to terminal restriction fragment Southern blot analysis under denaturing conditions with a (C 3 TA 2 ) 3 5′-end-radiolabeled oligonucleotide probe. Lanes: 1, HCT116 parental cell line; 2 and 3, HCT116 Ku86-heterozygous cell lines #44 and #70, respectively; 4, HCT116 p53-null cell line; 5 and 6, HCT116 p53-null Ku86-heterozygous cell lines #13 and #20, respectively. Approximate molecular size markers are shown on the far left.

    Journal: Molecular and Cellular Biology

    Article Title: Regulation of Telomere Length and Suppression of Genomic Instability in Human Somatic Cells by Ku86

    doi: 10.1128/MCB.24.11.5050-5059.2004

    Figure Lengend Snippet: Human Ku86-deficient cell lines have shortened telomeres. Genomic DNA was purified from the indicated cell lines, digested to completion with MboI and AluI, and then subjected to terminal restriction fragment Southern blot analysis under denaturing conditions with a (C 3 TA 2 ) 3 5′-end-radiolabeled oligonucleotide probe. Lanes: 1, HCT116 parental cell line; 2 and 3, HCT116 Ku86-heterozygous cell lines #44 and #70, respectively; 4, HCT116 p53-null cell line; 5 and 6, HCT116 p53-null Ku86-heterozygous cell lines #13 and #20, respectively. Approximate molecular size markers are shown on the far left.

    Article Snippet: TRF (terminal restriction fragment) and G-strand overhang assays were performed exactly as previously described ( , ), with the restriction enzymes MboI and AluI (New England Biolabs).

    Techniques: Purification, Southern Blot

    Yield of ligation products for different fragments of the mouse beta-globin gene domain. (A) The results for Hind III-digested fragments. (B) The results for Mbo I-digested fragments. Above each graph, a map of the domain is shown (beta-globin genes, red arrows; olfactory receptor genes, blue arrows; DNase I hypersensitive sites, black vertical lines). Black horizontal lines below the map show the positions and sizes of the analyzed restriction fragments. The individual fragments are designated by lowcase letters. An anchor symbol indicates the anchor restriction fragment. The ligation yield was calculated using the equation presented in Figure 2C . The ligation products are named according to the fragment designations; e/e–self-ligated product. The error bars represent the SEM of three independent experiments.

    Journal: PLoS ONE

    Article Title: Actual Ligation Frequencies in the Chromosome Conformation Capture Procedure

    doi: 10.1371/journal.pone.0060403

    Figure Lengend Snippet: Yield of ligation products for different fragments of the mouse beta-globin gene domain. (A) The results for Hind III-digested fragments. (B) The results for Mbo I-digested fragments. Above each graph, a map of the domain is shown (beta-globin genes, red arrows; olfactory receptor genes, blue arrows; DNase I hypersensitive sites, black vertical lines). Black horizontal lines below the map show the positions and sizes of the analyzed restriction fragments. The individual fragments are designated by lowcase letters. An anchor symbol indicates the anchor restriction fragment. The ligation yield was calculated using the equation presented in Figure 2C . The ligation products are named according to the fragment designations; e/e–self-ligated product. The error bars represent the SEM of three independent experiments.

    Article Snippet: The DNA was digested by overnight incubation with 600 units of Hind III or 800 units of Mbo I (New England Biolabs) at 37°C with shaking.

    Techniques: Ligation

    Effects of host plant, Aquilaria crassna (agarwood) and Tectona grandis (teak), and source of samples (root and soil) on mean number of terminal restriction fragments (TRFs) per sample using three restriction enzymes MboI (open bars), HinfI (hatched bars) and Hsp92II (cross-hatched bars). Values are mean ± SEM (n = 4 for teak and n = 3 for agarwood).

    Journal: PLoS ONE

    Article Title: Characterization of Arbuscular Mycorrhizal Fungus Communities of Aquilaria crassna and Tectona grandis Roots and Soils in Thailand Plantations

    doi: 10.1371/journal.pone.0112591

    Figure Lengend Snippet: Effects of host plant, Aquilaria crassna (agarwood) and Tectona grandis (teak), and source of samples (root and soil) on mean number of terminal restriction fragments (TRFs) per sample using three restriction enzymes MboI (open bars), HinfI (hatched bars) and Hsp92II (cross-hatched bars). Values are mean ± SEM (n = 4 for teak and n = 3 for agarwood).

    Article Snippet: The purified PCR products were digested separately with the selected restriction enzymes, HinfI, Hsp92II and MboI (Promega) , for 3 h at 37°C.

    Techniques:

    Occurrence of TRFs from roots and soils in (a) Tectona grandis and (b) Aquilaria crassna . Bars indicate the proportion of samples that yielded each TRF; dots indicate the average intensity of that fragment (± SEM) in those samples. The letters indicate the restriction enzyme involved in each fragment size, a: MboI , b: HinfI and c: Hsp92II .

    Journal: PLoS ONE

    Article Title: Characterization of Arbuscular Mycorrhizal Fungus Communities of Aquilaria crassna and Tectona grandis Roots and Soils in Thailand Plantations

    doi: 10.1371/journal.pone.0112591

    Figure Lengend Snippet: Occurrence of TRFs from roots and soils in (a) Tectona grandis and (b) Aquilaria crassna . Bars indicate the proportion of samples that yielded each TRF; dots indicate the average intensity of that fragment (± SEM) in those samples. The letters indicate the restriction enzyme involved in each fragment size, a: MboI , b: HinfI and c: Hsp92II .

    Article Snippet: The purified PCR products were digested separately with the selected restriction enzymes, HinfI, Hsp92II and MboI (Promega) , for 3 h at 37°C.

    Techniques:

    Peak abundance of Terminal Restriction Fragments (T-RFs) and AMF ribotype corresponds to fragment size. (A) Alu I, (B) Mbo I digestion.

    Journal: PLoS ONE

    Article Title: Arbuscular Mycorrhizal Fungi Community Structure, Abundance and Species Richness Changes in Soil by Different Levels of Heavy Metal and Metalloid Concentration

    doi: 10.1371/journal.pone.0128784

    Figure Lengend Snippet: Peak abundance of Terminal Restriction Fragments (T-RFs) and AMF ribotype corresponds to fragment size. (A) Alu I, (B) Mbo I digestion.

    Article Snippet: Purified PCR products were separately digested with the restriction enzyme Alu I and Mbo I (Promega, USA).

    Techniques:

    Identification of methylated recognition sequences in S. acidocaldarius genomic DNA by digestion assays. Restriction enzymes BsuRI (A) and BamHI, DpnI, and MboI (B) were used to highlight the presence/absence of methylated 5′-GGCC-3′ and 5′-GATC-3′ palindromes, respectively, in genomic DNA of S. acidocaldarius . Two types of substrates were digested: genomic DNA (gDNA) and a whole genome amplification of the genomic DNA (WGA). WGA is identical to gDNA in terms of nucleic sequence but it does not contain epigenetic marks (see material and methods). This negative control is only used in (A) . The addition of different restriction enzymes is symbolized by a positive sign “+” while reaction mix without restriction enzyme is represented by a negative sign “–”. Molecular size markers (GeneRuler DNA Ladder, Thermo Scientific) were loaded in both gels (M). Digestion patterns were obtained in 0.8% agarose gel stained with ethidium bromide and visualized under UV light.

    Journal: Frontiers in Microbiology

    Article Title: The DNA Methylome of the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

    doi: 10.3389/fmicb.2018.00137

    Figure Lengend Snippet: Identification of methylated recognition sequences in S. acidocaldarius genomic DNA by digestion assays. Restriction enzymes BsuRI (A) and BamHI, DpnI, and MboI (B) were used to highlight the presence/absence of methylated 5′-GGCC-3′ and 5′-GATC-3′ palindromes, respectively, in genomic DNA of S. acidocaldarius . Two types of substrates were digested: genomic DNA (gDNA) and a whole genome amplification of the genomic DNA (WGA). WGA is identical to gDNA in terms of nucleic sequence but it does not contain epigenetic marks (see material and methods). This negative control is only used in (A) . The addition of different restriction enzymes is symbolized by a positive sign “+” while reaction mix without restriction enzyme is represented by a negative sign “–”. Molecular size markers (GeneRuler DNA Ladder, Thermo Scientific) were loaded in both gels (M). Digestion patterns were obtained in 0.8% agarose gel stained with ethidium bromide and visualized under UV light.

    Article Snippet: Digestion assays Genomic DNA isolated from asynchronous S. acidocaldarius cells was cleaved independently with a specific REase in order to decipher the presence or the absence of m4C or m6A: BsuRI, BamHI, MboI, and DpnI (Thermo Scientific).

    Techniques: Methylation, Whole Genome Amplification, Sequencing, Negative Control, Agarose Gel Electrophoresis, Staining