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Image Search Results
Journal: bioRxiv
Article Title: Tau oligomers modulate synapse fate by eliciting progressive bipartite synapse dysregulation and synapse loss
doi: 10.1101/2025.09.19.677215
Figure Lengend Snippet: A Workflow indicating that the 30-min treatment with tau oligomers was followed by different intervals of time (30 min, 1 hour, 24 hours, 7, or 14 days) before the fixation of human neuron cultures for experiments. B Confocal images of human neurons treated with oligomerized tau conjugated with Fluorescein-5-isothiocyanate (tau-FITC) (green) showed colocalization (white arrowheads) of tau-FITC with Synapsin (blue) and PSD-95 (red). Scale bar: 5 μm. C Representative confocal images of human neurons at 30 min, 1 hour and 24 hours after the 30 min exposure with oligomerized tau-FITC as described in A . Scale bar: 5 μm. D Quantification of the integrated intensity of tau-FITC fluorescence at synapses in human neurons that were treated with oligomerized tau-FITC for 30 min and fixed at different time points A (n = 12 images/group; ** p < 0.01, *** p < 0.001, one-way ANOVA, Bonferroni post hoc analyses). E, F Representative images of NeuN immunolabeling, as a neuronal marker, in vehicle-treated control neurons and in neurons at either ( E ) 7 or ( F ) 14 days after the 30-min exposure to tau oligomers. The density of NeuN-positive cells in the cultures was calculated and compared with or without tau oligomer exposure (n = 12 images/group; no significant difference, Unpaired Student’s t -test). Scale bar: 100 mm. G, H Representative confocal images of MAP2 immunostaining in neurons at ( G ) 7 or ( H ) 14 days after the 30-min tau oligomer treatment and in neurons treated with vehicle control. Scale bars: 20 μm. The intensity of MAP2 immunolabeling in dendrites of human neurons was quantified at ( G ) 7 days (n = 12 images/group; no significant difference, Unpaired Student’s t -test) and ( H ) 14 days (n = 12 images/group; *** p < 0.001, Unpaired Student’s t -test) post-oligomer exposure. Values are given as means ± SEM.
Article Snippet: Primary antibodies used for western blot and immunocytochemistry were: Tau5 (AB_80579, Abcam), Streptavin HRP (S911, Invitrogen), GAPDH (MAB374, Sigma), Rabbit FLAG (F7425, Sigma), Mouse FLAG (F3165, Sigma), PSD-95 (MA1046, ThermoFisher), GluA2/3 (AB1506, MilliporeSigma), Cy3 Streptavidin (AB_2337244, Jackson Immunoresearch), Mouse vGluT1 (MAB5502, MilliporeSigma), Guinea Pig vGluT1 (AB5905, MilliporeSigma), GluA1 (ABN241, MilliporeSigma), GluN1 (114 011, SynapticSystems), Synapsin (5297S, Cell Signaling),
Techniques: Fluorescence, Immunolabeling, Marker, Control, Immunostaining