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Image Search Results

Journal: Drug Delivery
Article Title: Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes
doi: 10.1080/10717544.2022.2044937
Figure Lengend Snippet: Exosomes in each treatment group for activating DCs and anti-tumor T-cell immune response by flow cytometric analysis and immunofluorescence. (A) Schematic diagram of the experimental procedure. Exosomes (10 9 particles/mL) were injected three times at an interval of two days, and their dose was 2 × 10 9 particles/kg body weight per injection. Insonation (1 MHz, 2.0 W cm −2 , 20% duty cycle, 5 min) was applied two hours after administration. Tumors were excised for immunological analysis on day 16 after inoculation RM-1 cells. Flow cytometric analysis of (B) proportion of mature DCs (CD80 + CD86 + ) over CD11c + DCs and (D) CD8 + T cells over CD3 + T cells at the tumor tissues with different treatments. (C, E) Statistical results of (B) and (D), respectively ( n = 5). (F) Immunofluorescence staining of CD11c + CD86 + in tumor sections after different treatments. (H) Immunofluorescence staining of CD3 + CD8 + in tumor sections after different treatments. (G, I) Statistical results of (F) and (H), respectively ( n = 5). Data are expressed as mean ± SEM, one-way ANOVA, * p <.05, Exo Ce6 +US, Exo R848 , Exo Ce6+R848 +US versus Exo Ctrl ; # p <.05, Exo Ce6+R848 +US versus Exo R848 .
Article Snippet: For surface marker analysis, the cells were stained with APC-conjugated-anti-mouse CD11c (N418, Proteintech, Rosemont, IL), FITC-conjugated-anti-mouse CD3 (145-2C11, Proteintech, Rosemont, IL), PerCP-conjugated-anti-mouse CD4 (GK1.5, Biolegend, San Diego, CA), APC-conjugated-anti-mouse
Techniques: Immunofluorescence, Injection, Staining