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    No difference in the redox state of the bulk thiol proteome in unstimulated (control) and LPS-stimulated human monocytes. Significant differences (paired t-tests, P = < 0.0001 in panels A–B, n = 6) in percent reduced compared to percent oxidised protein in unstimulated (control, panel A) and LPS-stimulated (panel B) human monocytes. C. No significant difference (unpaired t -test, P = 0.9694, n = 6) in percent reversibly oxidised protein in LPS-stimulated human monocytes compared to unstimulated human monocytes. D. A representative SDS-PAGE gel image showing that both proteomes are highly reduced and similar band patterns in the reduced (red channel, <t>F-MAL1)</t> and reversibly oxidised (green channel, <t>F-MAL2)</t> channels in control and LPS samples. Data are presented as the mean (M) and standard deviation (SD). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Distribution localities of Raorchestes samples examined in the present study. Localities are arranged by State.
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    Distribution localities of Raorchestes samples examined in the present study. Localities are arranged by State.
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    Image Search Results


    No difference in the redox state of the bulk thiol proteome in unstimulated (control) and LPS-stimulated human monocytes. Significant differences (paired t-tests, P = < 0.0001 in panels A–B, n = 6) in percent reduced compared to percent oxidised protein in unstimulated (control, panel A) and LPS-stimulated (panel B) human monocytes. C. No significant difference (unpaired t -test, P = 0.9694, n = 6) in percent reversibly oxidised protein in LPS-stimulated human monocytes compared to unstimulated human monocytes. D. A representative SDS-PAGE gel image showing that both proteomes are highly reduced and similar band patterns in the reduced (red channel, F-MAL1) and reversibly oxidised (green channel, F-MAL2) channels in control and LPS samples. Data are presented as the mean (M) and standard deviation (SD). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Free Radical Biology & Medicine

    Article Title: RedoxiFluor: A microplate technique to quantify target-specific protein thiol redox state in relative percentage and molar terms

    doi: 10.1016/j.freeradbiomed.2022.01.023

    Figure Lengend Snippet: No difference in the redox state of the bulk thiol proteome in unstimulated (control) and LPS-stimulated human monocytes. Significant differences (paired t-tests, P = < 0.0001 in panels A–B, n = 6) in percent reduced compared to percent oxidised protein in unstimulated (control, panel A) and LPS-stimulated (panel B) human monocytes. C. No significant difference (unpaired t -test, P = 0.9694, n = 6) in percent reversibly oxidised protein in LPS-stimulated human monocytes compared to unstimulated human monocytes. D. A representative SDS-PAGE gel image showing that both proteomes are highly reduced and similar band patterns in the reduced (red channel, F-MAL1) and reversibly oxidised (green channel, F-MAL2) channels in control and LPS samples. Data are presented as the mean (M) and standard deviation (SD). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Samples were immediately lysed in IP buffer (25 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 5% glycerol, pH 7.1) supplemented with a protease inhibitor tablet (Sigma Aldrich, UK, #11697498001) and 1 mM F-MAL (F-MAL1: Fluorescin-5-maleimide, ThermoFisher, UK, #62245; or F-MAL2: AlexaFluor™647-C 2 -maleimide, ThermoFisher, UK; #A20347).

    Techniques: SDS Page, Standard Deviation

    Distribution localities of Raorchestes samples examined in the present study. Localities are arranged by State.

    Journal: PeerJ

    Article Title: An integrative approach to infer systematic relationships and define species groups in the shrub frog genus Raorchestes , with description of five new species from the Western Ghats, India

    doi: 10.7717/peerj.10791

    Figure Lengend Snippet: Distribution localities of Raorchestes samples examined in the present study. Localities are arranged by State.

    Article Snippet: Kannur , Paithal Mala , R. charius , R luteolus , R. tuberohumerus.

    Techniques: