magnetic oligo dt beads Thermo Fisher Search Results


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  • 83
    Thermo Fisher magnetic oligo dt beads
    Magnetic Oligo Dt Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dynabeads oligo dt magnetic beads
    Dynabeads Oligo Dt Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sera mag magnetic oligo dt beads
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Sera Mag Magnetic Oligo Dt Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 26228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cellulose oligo dt magnetic beads
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Cellulose Oligo Dt Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt selection
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Selection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt beads
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt tagged magnetic beads
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Tagged Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt coated magnetic beads
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Coated Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt magnetic bead separation
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Magnetic Bead Separation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt magnetic beads magnetic beads ambion poly
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Magnetic Beads Magnetic Beads Ambion Poly, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt dynal mrna direct kit dynal biotech oslo norway
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo Dt Dynal Mrna Direct Kit Dynal Biotech Oslo Norway, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher magnetic dyna beads oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Magnetic Dyna Beads Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher magnetic oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Magnetic Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sera mag magnetic oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Sera Mag Magnetic Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dynamag 2 magnet
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Dynamag 2 Magnet, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1057 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sera mag oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Sera Mag Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dyna oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Dyna Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dynabeads mrna direct kit
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Dynabeads Mrna Direct Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher poly a purist kit
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Poly A Purist Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dynabeads mrna purification kit
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Dynabeads Mrna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dynabeads mrna direct micro purification kit
    <t>Pre-mRNA</t> features that correlate with the extent of cotranscriptional splicing. ( A ) Cotranscriptional splicing levels differ based on gene position. The box plot shows the distribution of nRNA SPIs for the group of single intron genes and first, internal (second, third, and other), or last introns in multi-intron genes. The box width corresponds to the respective group size. ( B ) mRNA splicing levels differ between introns in different gene positions. Analogous data representation as in A . ( C ) One quarter of introns are significantly less or more spliced than the next downstream (3′) intron in nRNA, as depicted in a volcano plot (three biological replicates). ( D ) 15/42 analyzed gene architecture features correlate significantly with differentially spliced intron pairs (sequence-based in black font and RNA-seq-derived in gray font). The smaller intron position for “low” spliced introns in a pair (first intron – 1, second intron – 2, etc.) is consistent with enrichment of first introns in the “5′ less spliced” group. The median modified Z -score is shown for each feature with significant difference between the “low” and “high” groups, and the respective negative log 10 of the Bonferroni-corrected P -value is given. For two features, no change in the median modified Z -score is visible. The respective feature distribution is presented as a box plot below . Asterisks indicate significance of <t>direct</t> neighbors according to the Wilcoxon rank-sum test: (*) P
    Dynabeads Mrna Direct Micro Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and mRNA is isolated from this using oligo dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.

    Journal: Frontiers in Plant Science

    Article Title: A High-Throughput Method for Illumina RNA-Seq Library Preparation

    doi: 10.3389/fpls.2012.00202

    Figure Lengend Snippet: Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and mRNA is isolated from this using oligo dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.

    Article Snippet: For HTR library preparations, mRNA isolation with both Dynabeads (Invitrogen) and Sera-Mag oligo dT magnetic beads (Thermo Scientific, Cat. # 3815-2103-010150) were performed based on the Dynabeads mRNA direct kit (Invitrogen) protocol with minor adjustments (see Methods in Supplementary Material).

    Techniques: High Throughput Screening Assay, RNA Sequencing Assay, Lysis, Isolation, Ligation, Amplification, Purification, Sequencing

    Pre-mRNA features that correlate with the extent of cotranscriptional splicing. ( A ) Cotranscriptional splicing levels differ based on gene position. The box plot shows the distribution of nRNA SPIs for the group of single intron genes and first, internal (second, third, and other), or last introns in multi-intron genes. The box width corresponds to the respective group size. ( B ) mRNA splicing levels differ between introns in different gene positions. Analogous data representation as in A . ( C ) One quarter of introns are significantly less or more spliced than the next downstream (3′) intron in nRNA, as depicted in a volcano plot (three biological replicates). ( D ) 15/42 analyzed gene architecture features correlate significantly with differentially spliced intron pairs (sequence-based in black font and RNA-seq-derived in gray font). The smaller intron position for “low” spliced introns in a pair (first intron – 1, second intron – 2, etc.) is consistent with enrichment of first introns in the “5′ less spliced” group. The median modified Z -score is shown for each feature with significant difference between the “low” and “high” groups, and the respective negative log 10 of the Bonferroni-corrected P -value is given. For two features, no change in the median modified Z -score is visible. The respective feature distribution is presented as a box plot below . Asterisks indicate significance of direct neighbors according to the Wilcoxon rank-sum test: (*) P

    Journal: Genome Research

    Article Title: Long-read sequencing of nascent RNA reveals coupling among RNA processing events

    doi: 10.1101/gr.232025.117

    Figure Lengend Snippet: Pre-mRNA features that correlate with the extent of cotranscriptional splicing. ( A ) Cotranscriptional splicing levels differ based on gene position. The box plot shows the distribution of nRNA SPIs for the group of single intron genes and first, internal (second, third, and other), or last introns in multi-intron genes. The box width corresponds to the respective group size. ( B ) mRNA splicing levels differ between introns in different gene positions. Analogous data representation as in A . ( C ) One quarter of introns are significantly less or more spliced than the next downstream (3′) intron in nRNA, as depicted in a volcano plot (three biological replicates). ( D ) 15/42 analyzed gene architecture features correlate significantly with differentially spliced intron pairs (sequence-based in black font and RNA-seq-derived in gray font). The smaller intron position for “low” spliced introns in a pair (first intron – 1, second intron – 2, etc.) is consistent with enrichment of first introns in the “5′ less spliced” group. The median modified Z -score is shown for each feature with significant difference between the “low” and “high” groups, and the respective negative log 10 of the Bonferroni-corrected P -value is given. For two features, no change in the median modified Z -score is visible. The respective feature distribution is presented as a box plot below . Asterisks indicate significance of direct neighbors according to the Wilcoxon rank-sum test: (*) P

    Article Snippet: Poly(A)− RNA was obtained using oligo(dT)-coated magnetic beads binding to poly(A)+ RNA (Dynabeads mRNA DIRECT Micro Purification kit, Life Technologies). rRNA was removed from ∼5 µg chromatin-associated poly(A)− RNA using the Ribo-Zero Gold rRNA Removal kit (Yeast) from Epicentre/Illumina.

    Techniques: Sequencing, RNA Sequencing Assay, Derivative Assay, Modification