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  • 99
    New England Biolabs mgcl2
    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM <t>MgCl2</t> (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.
    Mgcl2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl2/product/New England Biolabs
    Average 99 stars, based on 9116 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Thermo Fisher mgcl2 vwr
    <t>MgCl2:</t> Melt® results.
    Mgcl2 Vwr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl2 vwr/product/Thermo Fisher
    Average 99 stars, based on 14366 article reviews
    Price from $9.99 to $1999.99
    mgcl2 vwr - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Millipore magnesium chloride mgcl2
    PPM1H dephosphorylates Rab10 in vitro. ( A ) The indicated amounts of recombinant wild-type and mutant PPM1H (with a His-Sumo N-terminal tag, expressed in E. coli ) were incubated in vitro with 2.5 µg pT73 ~60% phosphorylated Rab10[1–181, GDP bound] for 30 min in the presence of 10 mM <t>MgCl2</t> in 40 mM HEPES pH 7.0 buffer. Reactions were terminated by addition of SDS Sample Buffer and analyzed by phos-tag gel electrophoresis that separates phosphorylated and dephosphorylated Rab10. Gel was stained with Instant Blue Coomassie. Bands corresponding to phosphorylated and non-phosphorylated Rab10 are marked with open (○) and closed (●) circles respectively. ( B ) As in ( A ) except that a time-course assay was performed using 2.5 µg pT73 phosphorylated Rab10[1–181, GDP bound] and 40 ng wild-type or mutant PPM1H for the indicated times. ( C ) As in ( A ) except that PPM1J was assessed. ( D ) As in ( A ) except and PPM1M was assessed.
    Magnesium Chloride Mgcl2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium chloride mgcl2/product/Millipore
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    magnesium chloride mgcl2 - by Bioz Stars, 2020-07
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      Buy from Supplier

    Image Search Results


    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Journal: Journal of Insect Science

    Article Title: Rapid Identification of Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) Using Ribosomal RNA Internal Transcribed Spacer 1

    doi: 10.1093/jisesa/iev137

    Figure Lengend Snippet: Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Article Snippet: LongAmp Taq polymerase and reaction buffer with 2.5 mM MgCl2 (New England Biolabs) produced dissociation curves with lower intensity than ready-to-use master mixes but sufficient to detect H. armigera in 1:24 ratio of H. armigera : H. zea tissue lysate ( a and b).

    Techniques: Produced, Modification, Amplification

    MgCl2: Melt® results.

    Journal: Biological Procedures Online

    Article Title: Rapid and simple comparison of messenger RNA levels using real-time PCR

    doi: 10.1251/bpo114

    Figure Lengend Snippet: MgCl2: Melt® results.

    Article Snippet: Each sample consisted of: 50 ng cDNA, 3 mM MgCl2 , 200 μM dNTP, 500 nM of primers, 2 μl of 10X PCR buffer, 0.1 unit of Taq polymerase and SYBR® Green (Molecular Probe, Eugene, OR; 1/30 000 dilution), in a reaction volume of 20 μl.

    Techniques:

    Optimization of MgCl2 concentration in samples.

    Journal: Biological Procedures Online

    Article Title: Rapid and simple comparison of messenger RNA levels using real-time PCR

    doi: 10.1251/bpo114

    Figure Lengend Snippet: Optimization of MgCl2 concentration in samples.

    Article Snippet: Each sample consisted of: 50 ng cDNA, 3 mM MgCl2 , 200 μM dNTP, 500 nM of primers, 2 μl of 10X PCR buffer, 0.1 unit of Taq polymerase and SYBR® Green (Molecular Probe, Eugene, OR; 1/30 000 dilution), in a reaction volume of 20 μl.

    Techniques: Concentration Assay

    PPM1H dephosphorylates Rab10 in vitro. ( A ) The indicated amounts of recombinant wild-type and mutant PPM1H (with a His-Sumo N-terminal tag, expressed in E. coli ) were incubated in vitro with 2.5 µg pT73 ~60% phosphorylated Rab10[1–181, GDP bound] for 30 min in the presence of 10 mM MgCl2 in 40 mM HEPES pH 7.0 buffer. Reactions were terminated by addition of SDS Sample Buffer and analyzed by phos-tag gel electrophoresis that separates phosphorylated and dephosphorylated Rab10. Gel was stained with Instant Blue Coomassie. Bands corresponding to phosphorylated and non-phosphorylated Rab10 are marked with open (○) and closed (●) circles respectively. ( B ) As in ( A ) except that a time-course assay was performed using 2.5 µg pT73 phosphorylated Rab10[1–181, GDP bound] and 40 ng wild-type or mutant PPM1H for the indicated times. ( C ) As in ( A ) except that PPM1J was assessed. ( D ) As in ( A ) except and PPM1M was assessed.

    Journal: eLife

    Article Title: PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins

    doi: 10.7554/eLife.50416

    Figure Lengend Snippet: PPM1H dephosphorylates Rab10 in vitro. ( A ) The indicated amounts of recombinant wild-type and mutant PPM1H (with a His-Sumo N-terminal tag, expressed in E. coli ) were incubated in vitro with 2.5 µg pT73 ~60% phosphorylated Rab10[1–181, GDP bound] for 30 min in the presence of 10 mM MgCl2 in 40 mM HEPES pH 7.0 buffer. Reactions were terminated by addition of SDS Sample Buffer and analyzed by phos-tag gel electrophoresis that separates phosphorylated and dephosphorylated Rab10. Gel was stained with Instant Blue Coomassie. Bands corresponding to phosphorylated and non-phosphorylated Rab10 are marked with open (○) and closed (●) circles respectively. ( B ) As in ( A ) except that a time-course assay was performed using 2.5 µg pT73 phosphorylated Rab10[1–181, GDP bound] and 40 ng wild-type or mutant PPM1H for the indicated times. ( C ) As in ( A ) except that PPM1J was assessed. ( D ) As in ( A ) except and PPM1M was assessed.

    Article Snippet: The protein was eluted with 4 × 1 resin volume of elution buffer (30 mM Tris pH 7.5, 3% (by vol) glycerol, 0.4 M imidazole, 8 mM 2-mercaptoethanol, 1.2 mM MgCl2 , 0.02% (by vol) Brij35, 0.6 µM GTP-gamma-S) and dialyzed against 50 mM Tris pH 7.5, 10% (by vol) glycerol, 250 mM NaCl, 14 mM 2-mercaptoethanol, 2 mM MgCl2 , 0.6 µM GTP-gamma-S in the presence of 60 Units of Thrombin (Sigma-Aldrich T1063-1KU).

    Techniques: In Vitro, Recombinant, Mutagenesis, Incubation, Nucleic Acid Electrophoresis, Staining