Journal: Biomedicines

Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

doi: 10.3390/biomedicines10071524

Figure Lengend Snippet: Discriminating M1 and M2-specific macrophage populations by surface determinants and secreted interleukins/chemokines as reported in [ 2 , 9 , 17 , 21 , 25 , 26 , 27 ] and used in the present study to determine M1 and M2-lineage specific macrophage populations.

Article Snippet: Monocyte attachment medium, macrophage detachment solution, M1 macrophages and M2 macrophage generation medium were from PromoCell (Heidelberg, Germany) and used according to the manufacturer’s instruction.

Techniques:

Journal: Biomedicines

Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

doi: 10.3390/biomedicines10071524

Figure Lengend Snippet: Phenotypic analysis of macrophages differentiated from adherent cells isolated from human peripheral blood and cultured for 6 days. M0, M1 and M2 macrophage phenotypes were obtained by culturing cells in basal medium, medium containing GM-CSF and M-CSF, respectively. Levels of CD11b ( A ), CD14 ( B ), CD209 ( C ), CD274 ( D ), CCR7 ( E ) and HLA-DR ( F ) expression in cells cultured in basal medium (red, M0 macrophages), GM-CSF (green, M1 macrophages) or M-CSF (blue, M2 macrophages) Substances induced MFI intensity differences of ≈2-fold (e.g., CD274 ( D )) to >100-fold (e.g., CD14 ( B )). Staining with isotype control antibodies: light grey. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves (see also ). Only surface determinants where marked differences between cultured conditions were observed are shown.

Article Snippet: Monocyte attachment medium, macrophage detachment solution, M1 macrophages and M2 macrophage generation medium were from PromoCell (Heidelberg, Germany) and used according to the manufacturer’s instruction.

Techniques: Isolation, Cell Culture, Expressing, Staining

Journal: Biomedicines

Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

doi: 10.3390/biomedicines10071524

Figure Lengend Snippet: Res and ω-3 PUFAs alter the expression of surface determinants on M1 (GM-CSF) and M2 (M-CSF) macrophages differentiated from peripheral blood mononuclear cells. Adherent cells isolated from peripheral blood were cultured in medium containing GM-CSF to M1 macrophages ( A – D ) or M-CSF to M2 macrophages ( E – H ) with Res (red) or ω-3 PUFAs (green) or without substances (black) for 6 days. Levels of CD14 ( A , E ), CD206 ( B , F ), CD274 ( C , G ) and HLA-DR ( D , H ) were determined by cytofluorometry. MFI values are indicated for the major peak of the colour-coded cytofluorometric curves (see also ).

Article Snippet: Monocyte attachment medium, macrophage detachment solution, M1 macrophages and M2 macrophage generation medium were from PromoCell (Heidelberg, Germany) and used according to the manufacturer’s instruction.

Techniques: Expressing, Isolation, Cell Culture

Journal: Biomedicines

Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

doi: 10.3390/biomedicines10071524

Figure Lengend Snippet: Secretion of cytokines and chemokines by macrophages differentiated from PBMCs. Adherent cells isolated from peripheral blood were cultured in GM-CSF (open bars) and M-CSF (hatched bars) for M1 and M2 differentiation, respectively, for 7 days, followed by activation with LPS/IFN-γ or IL-4/IL-13 for 24 h. ( A ) TNF-α ( B ) IL-1β, ( C ) IL-6, ( D ) IL-10, ( E ) IL-12p70, ( F ) CXCL10/IP-10, ( G ) CCL13/MCP-4, ( H ) CCL18/PARC, ( I ) CCL20/MIP-3α, ( K ) CXCL1/GRO-α. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of four performed) are given. * p < 0.05, ** p < 0.01 (vs. activated cells).

Article Snippet: Monocyte attachment medium, macrophage detachment solution, M1 macrophages and M2 macrophage generation medium were from PromoCell (Heidelberg, Germany) and used according to the manufacturer’s instruction.

Techniques: Isolation, Cell Culture, Activation Assay, Standard Deviation

Journal: Biomedicines

Article Title: Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

doi: 10.3390/biomedicines10071524

Figure Lengend Snippet: Secretion of chemokines and cytokines by PBMC cultured for 7 days in basal medium (M0), GM-CSF (M1 polarized) and M-CSF (M2-polarized). Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of two performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN- γ induced secretion versus IL-4/IL-13 induced secretion. Secretion of chemokines and cytokines by PBMC cultured for 7 days in GM-CSF (M1-polarized) and M-CSF (M2-polarized). M1-polarized cells and M2-polarized cells were activated with LPS/IFN-γ and IL-4/IL-13, respectively, for 24 h. Data show the secreted metabolites during the 24 h activation period. Mean values ± standard deviation [pg/mL] of triplicates of a representative experiment (of three performed) are given. The M1/M2 ratio returns to the quotient of LPS/IFN- γ induced secretion versus IL-4/IL-13 induced secretion.

Article Snippet: Monocyte attachment medium, macrophage detachment solution, M1 macrophages and M2 macrophage generation medium were from PromoCell (Heidelberg, Germany) and used according to the manufacturer’s instruction.

Techniques: Cell Culture, Standard Deviation, Activation Assay