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  • 88
    GenXPro mace libraries
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Libraries, supplied by GenXPro, used in various techniques. Bioz Stars score: 88/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace libraries/product/GenXPro
    Average 88 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    mace libraries - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Illumina Inc mace libraries
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace libraries/product/Illumina Inc
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    mace libraries - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    GenXPro cdna ends mace mace libraries
    qRT-PCR validation of selected DEGs detected to be associated with pod dehiscence. (A) <t>MACE</t> results in comparison with qRT-PCR of selected candidate genes. MACE-P004, MACE-P013, and MACE-P015 shows the same trend of over/down expression between MACE results of parental lines and RILs (JI 64—dehiscent pod, bulk of dehiscent RILs, JI 92—indehiscent pod, bulk of indehiscent RILs) and qRT-PCR of <t>cDNA</t> of parental lines (ventral or dorsal pod suture in two stages: I—younger stage, II—older stage). MACE-P009 showed the opposite results and MACE-P017 and MACE-P0018 without any trend of down or over expression in qRT-PCR. (B) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate gene MACE-P015. (C) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate genes SHATTERPROOF and SHATTERING.
    Cdna Ends Mace Mace Libraries, supplied by GenXPro, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna ends mace mace libraries/product/GenXPro
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cdna ends mace mace libraries - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Journal: Cell Death & Disease

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    doi: 10.1038/cddis.2017.44

    Figure Lengend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

    Techniques: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing

    qRT-PCR validation of selected DEGs detected to be associated with pod dehiscence. (A) MACE results in comparison with qRT-PCR of selected candidate genes. MACE-P004, MACE-P013, and MACE-P015 shows the same trend of over/down expression between MACE results of parental lines and RILs (JI 64—dehiscent pod, bulk of dehiscent RILs, JI 92—indehiscent pod, bulk of indehiscent RILs) and qRT-PCR of cDNA of parental lines (ventral or dorsal pod suture in two stages: I—younger stage, II—older stage). MACE-P009 showed the opposite results and MACE-P017 and MACE-P0018 without any trend of down or over expression in qRT-PCR. (B) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate gene MACE-P015. (C) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate genes SHATTERPROOF and SHATTERING.

    Journal: Frontiers in Plant Science

    Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)

    doi: 10.3389/fpls.2017.00542

    Figure Lengend Snippet: qRT-PCR validation of selected DEGs detected to be associated with pod dehiscence. (A) MACE results in comparison with qRT-PCR of selected candidate genes. MACE-P004, MACE-P013, and MACE-P015 shows the same trend of over/down expression between MACE results of parental lines and RILs (JI 64—dehiscent pod, bulk of dehiscent RILs, JI 92—indehiscent pod, bulk of indehiscent RILs) and qRT-PCR of cDNA of parental lines (ventral or dorsal pod suture in two stages: I—younger stage, II—older stage). MACE-P009 showed the opposite results and MACE-P017 and MACE-P0018 without any trend of down or over expression in qRT-PCR. (B) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate gene MACE-P015. (C) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate genes SHATTERPROOF and SHATTERING.

    Article Snippet: Massive analysis of cDNA ends (MACE) MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ).

    Techniques: Quantitative RT-PCR, Expressing, Over Expression