mab 2c1 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Hycult Biotech alpha-1-antitrypsin, human, mab 2c1
    Alpha 1 Antitrypsin, Human, Mab 2c1, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alpha-1-antitrypsin, human, mab 2c1/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alpha-1-antitrypsin, human, mab 2c1 - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    86
    Bio-Rad mab pak3 2c1
    Mab Pak3 2c1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mab pak3 2c1/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mab pak3 2c1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    GeneTex anti atm mab 2c1
    Nek1 expression, kinase activity and IR-induced localization of Nek1 to nuclear foci are intact in <t>Atm</t> null mouse renal tubular epithelial cells or ATR null human cells. (A–C) Nek1 activity in ATM null cells. Atm+/+ and Atm−/− renal tubular epithelial cells were established from 6-week-old mice as described previously in references 5 and 6, and used for Nek1 activity analysis. (A) Nek1 expression in Atm−/− cells. 1 h after IR (10 Gy), cells were lysed and protein lysates were separated by SDS gel electrophoresis, transferred to membranes, and western blotted <t>with</t> <t>anti-Atm</t> (upper part), anti-Nek1 (middle part) and anti-p84 (lower part) antibodies, the latter to control for loading. (B), Nek1 kinase activity is upregulated after DNA damage by IR (10 Gy) in Atm null cells. Activity was measured using γ-32P-ATP, β-casein as an in vitro substrate and Nek1 immune complexes from Atm+/+ (lanes 1 and 2) or Atm−/− cells (lanes 3 and 4), as described previously in reference 6. (C) Nek1 localizes to IRIF in Atm null cells. Cells were fixed 1 hour after irradiation with IR (10 Gy). The fixed cells were immunostained with anti-Nek1 antibodies.
    Anti Atm Mab 2c1, supplied by GeneTex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti atm mab 2c1/product/GeneTex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti atm mab 2c1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Amgen 2c1 1 primary mab
    Nek1 expression, kinase activity and IR-induced localization of Nek1 to nuclear foci are intact in <t>Atm</t> null mouse renal tubular epithelial cells or ATR null human cells. (A–C) Nek1 activity in ATM null cells. Atm+/+ and Atm−/− renal tubular epithelial cells were established from 6-week-old mice as described previously in references 5 and 6, and used for Nek1 activity analysis. (A) Nek1 expression in Atm−/− cells. 1 h after IR (10 Gy), cells were lysed and protein lysates were separated by SDS gel electrophoresis, transferred to membranes, and western blotted <t>with</t> <t>anti-Atm</t> (upper part), anti-Nek1 (middle part) and anti-p84 (lower part) antibodies, the latter to control for loading. (B), Nek1 kinase activity is upregulated after DNA damage by IR (10 Gy) in Atm null cells. Activity was measured using γ-32P-ATP, β-casein as an in vitro substrate and Nek1 immune complexes from Atm+/+ (lanes 1 and 2) or Atm−/− cells (lanes 3 and 4), as described previously in reference 6. (C) Nek1 localizes to IRIF in Atm null cells. Cells were fixed 1 hour after irradiation with IR (10 Gy). The fixed cells were immunostained with anti-Nek1 antibodies.
    2c1 1 Primary Mab, supplied by Amgen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2c1 1 primary mab/product/Amgen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2c1 1 primary mab - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    GeneTex mouse monoclonal 2c1
    Nek1 expression, kinase activity and IR-induced localization of Nek1 to nuclear foci are intact in <t>Atm</t> null mouse renal tubular epithelial cells or ATR null human cells. (A–C) Nek1 activity in ATM null cells. Atm+/+ and Atm−/− renal tubular epithelial cells were established from 6-week-old mice as described previously in references 5 and 6, and used for Nek1 activity analysis. (A) Nek1 expression in Atm−/− cells. 1 h after IR (10 Gy), cells were lysed and protein lysates were separated by SDS gel electrophoresis, transferred to membranes, and western blotted <t>with</t> <t>anti-Atm</t> (upper part), anti-Nek1 (middle part) and anti-p84 (lower part) antibodies, the latter to control for loading. (B), Nek1 kinase activity is upregulated after DNA damage by IR (10 Gy) in Atm null cells. Activity was measured using γ-32P-ATP, β-casein as an in vitro substrate and Nek1 immune complexes from Atm+/+ (lanes 1 and 2) or Atm−/− cells (lanes 3 and 4), as described previously in reference 6. (C) Nek1 localizes to IRIF in Atm null cells. Cells were fixed 1 hour after irradiation with IR (10 Gy). The fixed cells were immunostained with anti-Nek1 antibodies.
    Mouse Monoclonal 2c1, supplied by GeneTex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal 2c1/product/GeneTex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal 2c1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Nek1 expression, kinase activity and IR-induced localization of Nek1 to nuclear foci are intact in Atm null mouse renal tubular epithelial cells or ATR null human cells. (A–C) Nek1 activity in ATM null cells. Atm+/+ and Atm−/− renal tubular epithelial cells were established from 6-week-old mice as described previously in references 5 and 6, and used for Nek1 activity analysis. (A) Nek1 expression in Atm−/− cells. 1 h after IR (10 Gy), cells were lysed and protein lysates were separated by SDS gel electrophoresis, transferred to membranes, and western blotted with anti-Atm (upper part), anti-Nek1 (middle part) and anti-p84 (lower part) antibodies, the latter to control for loading. (B), Nek1 kinase activity is upregulated after DNA damage by IR (10 Gy) in Atm null cells. Activity was measured using γ-32P-ATP, β-casein as an in vitro substrate and Nek1 immune complexes from Atm+/+ (lanes 1 and 2) or Atm−/− cells (lanes 3 and 4), as described previously in reference 6. (C) Nek1 localizes to IRIF in Atm null cells. Cells were fixed 1 hour after irradiation with IR (10 Gy). The fixed cells were immunostained with anti-Nek1 antibodies.

    Journal: Cell Cycle

    Article Title: Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR

    doi: 10.4161/cc.10.4.14814

    Figure Lengend Snippet: Nek1 expression, kinase activity and IR-induced localization of Nek1 to nuclear foci are intact in Atm null mouse renal tubular epithelial cells or ATR null human cells. (A–C) Nek1 activity in ATM null cells. Atm+/+ and Atm−/− renal tubular epithelial cells were established from 6-week-old mice as described previously in references 5 and 6, and used for Nek1 activity analysis. (A) Nek1 expression in Atm−/− cells. 1 h after IR (10 Gy), cells were lysed and protein lysates were separated by SDS gel electrophoresis, transferred to membranes, and western blotted with anti-Atm (upper part), anti-Nek1 (middle part) and anti-p84 (lower part) antibodies, the latter to control for loading. (B), Nek1 kinase activity is upregulated after DNA damage by IR (10 Gy) in Atm null cells. Activity was measured using γ-32P-ATP, β-casein as an in vitro substrate and Nek1 immune complexes from Atm+/+ (lanes 1 and 2) or Atm−/− cells (lanes 3 and 4), as described previously in reference 6. (C) Nek1 localizes to IRIF in Atm null cells. Cells were fixed 1 hour after irradiation with IR (10 Gy). The fixed cells were immunostained with anti-Nek1 antibodies.

    Article Snippet: 5 Anti-p84 mAb 5E10, 42 anti-p48, anti-ATR mAb 2B5 and anti-ATM mAb 2C1 were purchased from GeneTex Inc., (Irvine, CA).

    Techniques: Expressing, Activity Assay, SDS-Gel, Electrophoresis, Western Blot, In Vitro, Irradiation

    Phosphorylation of ATM-dependent substrates is intact in Nek1 null cells. (A and B) Activation of ATM and ATR are intact in Nek1−/− cells. Cells were treated with IR (10 Gy) or UV (10 J/m2) irradiation. Total protein lysates were subjected to SDS-PAGE, and then immunblotted with anti-Atm (A) or Atr (B) antibodies. (C) Antibodies recognizing activated ATM-phospho-S1987, phospho-S/T-Q substrate and γ-H2AX were used for indirect immunofluorescence staining of wild-type and Nek1−/− renal tubular cells cultured from littermate kat2J mice. One hour after IR, activated Atm, phospho-S/T-Q Atm substrate and γ-H2AX all were present in nuclear foci at sites of DNA damage in wild-type cells and in Nek1−/− cells. Percentages (means ± SEM) were determined by scoring >400 individual cell nuclei for the presence of >5 IRIF, then dividing the number of positive cells by the total number of nuclei stained with DAPI.44

    Journal: Cell Cycle

    Article Title: Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR

    doi: 10.4161/cc.10.4.14814

    Figure Lengend Snippet: Phosphorylation of ATM-dependent substrates is intact in Nek1 null cells. (A and B) Activation of ATM and ATR are intact in Nek1−/− cells. Cells were treated with IR (10 Gy) or UV (10 J/m2) irradiation. Total protein lysates were subjected to SDS-PAGE, and then immunblotted with anti-Atm (A) or Atr (B) antibodies. (C) Antibodies recognizing activated ATM-phospho-S1987, phospho-S/T-Q substrate and γ-H2AX were used for indirect immunofluorescence staining of wild-type and Nek1−/− renal tubular cells cultured from littermate kat2J mice. One hour after IR, activated Atm, phospho-S/T-Q Atm substrate and γ-H2AX all were present in nuclear foci at sites of DNA damage in wild-type cells and in Nek1−/− cells. Percentages (means ± SEM) were determined by scoring >400 individual cell nuclei for the presence of >5 IRIF, then dividing the number of positive cells by the total number of nuclei stained with DAPI.44

    Article Snippet: 5 Anti-p84 mAb 5E10, 42 anti-p48, anti-ATR mAb 2B5 and anti-ATM mAb 2C1 were purchased from GeneTex Inc., (Irvine, CA).

    Techniques: Activation Assay, Irradiation, SDS Page, Immunofluorescence, Staining, Cell Culture