m220 Covaris Search Results


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    Covaris covaris m220
    Comparison of three Illumina library construction protocols. <t>Covaris</t> <t>M220</t> was used to fragment DNA in TruSeq Nano.
    Covaris M220, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 1126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris m220/product/Covaris
    Average 99 stars, based on 1126 article reviews
    Price from $9.99 to $1999.99
    covaris m220 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher covaris m220
    Comparison of three Illumina library construction protocols. <t>Covaris</t> <t>M220</t> was used to fragment DNA in TruSeq Nano.
    Covaris M220, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris m220/product/Thermo Fisher
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    covaris m220 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    Gene Company Ltd covaris m220
    Comparison of three Illumina library construction protocols. <t>Covaris</t> <t>M220</t> was used to fragment DNA in TruSeq Nano.
    Covaris M220, supplied by Gene Company Ltd, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris m220/product/Gene Company Ltd
    Average 93 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    covaris m220 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    Gene Company Ltd covaris m220 sonicator
    Comparison of three Illumina library construction protocols. <t>Covaris</t> <t>M220</t> was used to fragment DNA in TruSeq Nano.
    Covaris M220 Sonicator, supplied by Gene Company Ltd, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris m220 sonicator/product/Gene Company Ltd
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    covaris m220 sonicator - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of three Illumina library construction protocols. Covaris M220 was used to fragment DNA in TruSeq Nano.

    Journal: Human immunology

    Article Title: Impact of Three Illumina Library Construction Methods on GC Bias and HLA Genotype Calling

    doi: 10.1016/j.humimm.2014.12.016

    Figure Lengend Snippet: Comparison of three Illumina library construction protocols. Covaris M220 was used to fragment DNA in TruSeq Nano.

    Article Snippet: Aiming for an insert size of 550 bp, 200 ng of pooled PCR products were sheared by sonication using Covaris M220 (Covaris, Woburn, MA) with duty factor 20%, peak incident power 50 W, and 200 cycles per burst for 60 sec. Amplicon fragments underwent further purification with Agencourt AMPure XP beads in 1.6× reaction volume.

    Techniques:

    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Journal: Genes

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples

    doi: 10.3390/genes9010049

    Figure Lengend Snippet: Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Article Snippet: Nuclear DNA Sensitivity and Size Selection Study The single source male control DNA NA24129 (Coriell Institute for Medical Research, Camden, NJ, USA) was mechanically fragmented using the Covaris® M220 Focused-ultrasonicatorTM (Covaris, Woburn, MA, USA) to an average size of 175 bp with a range from 25 bp to 250 bp.

    Techniques: Next-Generation Sequencing, Sequencing, Amplification