m13 plus-strand genomic dna Search Results


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    Thermo Fisher 3730 dna genetic analyzer
    <t>TYLCV</t> ( A ) and TYLCV mutant ( B ) could be transmitted by the whitefly B. tabaci MED. ( C ) Detection of TYLCV <t>DNA</t> from tomato leaf tissues transmitted by whitefly at 30 days.
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    Thermo Fisher bp dna ladder
    <t>TYLCV</t> ( A ) and TYLCV mutant ( B ) could be transmitted by the whitefly B. tabaci MED. ( C ) Detection of TYLCV <t>DNA</t> from tomato leaf tissues transmitted by whitefly at 30 days.
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    Image Search Results


    TYLCV ( A ) and TYLCV mutant ( B ) could be transmitted by the whitefly B. tabaci MED. ( C ) Detection of TYLCV DNA from tomato leaf tissues transmitted by whitefly at 30 days.

    Journal: Scientific Reports

    Article Title: Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci

    doi: 10.1038/s41598-017-16330-4

    Figure Lengend Snippet: TYLCV ( A ) and TYLCV mutant ( B ) could be transmitted by the whitefly B. tabaci MED. ( C ) Detection of TYLCV DNA from tomato leaf tissues transmitted by whitefly at 30 days.

    Article Snippet: Full length TYLCV sequences of these clones were determined using an automated DNA sequencer (Model 3730, Applied Biosystems) using the M13 forward and reverse primers, and a pair of walking primers TYLCV-W/F (5′-TCTGCAATCCAGGACCTACC-3′, positioned from bases 1717 to 1698) and TYLCV-W/R (5′-AGTCTATCTTGCAATATGTG-3′, positioned from bases 133 to 152) as previously described .

    Techniques: Mutagenesis

    Agroinoculation-mediated test of the pathogenicity of TYLCV and TYLCV mutant. ( A ) TYLCV mutant attenuates the symptoms induced by TYLCV. Tomato and N. benthamiana plants were agro-inoculated with TYLCV or TYLCV mutant (Δ TYLCV), and were photographed 30 days later. ( B ) DNA gel blot hybridization analysis of TYLCV DNA from systemically infected tomato leaf tissues. 20 μg of total DNA was used for each lane. An ethidium bromide stained gel was provided as a loading control. ( C ) Triple antibody sandwich ELISA test of TYLCV viral content in TYLCV or TYLCV mutant-infected tomato and N. benthamiana plants, respectively. A value represents the mean value obtained from four independent plants with three replicates at OD 405 . The error bars indicate the standard deviation of each sample. * p

    Journal: Scientific Reports

    Article Title: Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci

    doi: 10.1038/s41598-017-16330-4

    Figure Lengend Snippet: Agroinoculation-mediated test of the pathogenicity of TYLCV and TYLCV mutant. ( A ) TYLCV mutant attenuates the symptoms induced by TYLCV. Tomato and N. benthamiana plants were agro-inoculated with TYLCV or TYLCV mutant (Δ TYLCV), and were photographed 30 days later. ( B ) DNA gel blot hybridization analysis of TYLCV DNA from systemically infected tomato leaf tissues. 20 μg of total DNA was used for each lane. An ethidium bromide stained gel was provided as a loading control. ( C ) Triple antibody sandwich ELISA test of TYLCV viral content in TYLCV or TYLCV mutant-infected tomato and N. benthamiana plants, respectively. A value represents the mean value obtained from four independent plants with three replicates at OD 405 . The error bars indicate the standard deviation of each sample. * p

    Article Snippet: Full length TYLCV sequences of these clones were determined using an automated DNA sequencer (Model 3730, Applied Biosystems) using the M13 forward and reverse primers, and a pair of walking primers TYLCV-W/F (5′-TCTGCAATCCAGGACCTACC-3′, positioned from bases 1717 to 1698) and TYLCV-W/R (5′-AGTCTATCTTGCAATATGTG-3′, positioned from bases 133 to 152) as previously described .

    Techniques: Mutagenesis, Western Blot, Hybridization, Infection, Staining, Sandwich ELISA, Standard Deviation