m. smegmatis Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    ATCC m smegmatis
    Southern analysis with an erm (38)-specific probe. Lanes: S1, M. <t>smegmatis</t> mc 2 155; S2, M. smegmatis ATCC 14468; F, M. fortuitum ATCC 6841; A, M. abscessus MAB30; C, M. chelonae ATCC 35752; M, M. microti ATCC 19422; B, M. bovis BCG.
    M Smegmatis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/ATCC
    Average 99 stars, based on 457 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    Wakamoto m smegmatis
    Relationship between birth length and other single cell measures for single M. <t>smegmatis</t> cells grown with glycerol as a sole carbon source. Red circles, single cell measurements; histograms, distribution of individual cells, with kernel density estimates depicted as a blue line; solid line with white filled circles, mean of birth length bins with error bars denoting the 95% confidence interval of the estimate determined by bootstrapping; gray dotted line, the least squares linear regression fit of unbinned data; Key, slope —the slope of the regression line (± the 95% confidence interval), intercept —the y -intercept, r —the Pearson correlation coefficient, n —the total number of cells measured, (A) Division length is plotted against birth length. (B) Interdivision time against birth length. (C) Added length against exponential growth rate. (D) Exponential growth rate against birth length.
    M Smegmatis, supplied by Wakamoto, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Wakamoto
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    91
    MiddleBrook Pharmaceuticals m smegmatis m smegmatis
    The effect of standard antimycobacterial agents on the growth of M. <t>smegmatis</t> . M. smegmatis mc 2 155 was treated with different concentrations of isoniazid ( a ) and kanamycin ( b ) from 0 μg/ml to 100 μg/ml. The bacteria were incubated with drugs at 37 ˚C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm
    M Smegmatis M Smegmatis, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis m smegmatis/product/MiddleBrook Pharmaceuticals
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    m smegmatis m smegmatis - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    94
    Constant Systems m smegmatis pellets
    The effect of standard antimycobacterial agents on the growth of M. <t>smegmatis</t> . M. smegmatis mc 2 155 was treated with different concentrations of isoniazid ( a ) and kanamycin ( b ) from 0 μg/ml to 100 μg/ml. The bacteria were incubated with drugs at 37 ˚C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm
    M Smegmatis Pellets, supplied by Constant Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis pellets/product/Constant Systems
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m smegmatis pellets - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    87
    InvivoGen m smegmatis pilam
    The effect of standard antimycobacterial agents on the growth of M. <t>smegmatis</t> . M. smegmatis mc 2 155 was treated with different concentrations of isoniazid ( a ) and kanamycin ( b ) from 0 μg/ml to 100 μg/ml. The bacteria were incubated with drugs at 37 ˚C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm
    M Smegmatis Pilam, supplied by InvivoGen, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis pilam/product/InvivoGen
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m smegmatis pilam - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    85
    Millipore mutanolysin treated m smegmatis
    The effect of standard antimycobacterial agents on the growth of M. <t>smegmatis</t> . M. smegmatis mc 2 155 was treated with different concentrations of isoniazid ( a ) and kanamycin ( b ) from 0 μg/ml to 100 μg/ml. The bacteria were incubated with drugs at 37 ˚C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm
    Mutanolysin Treated M Smegmatis, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mutanolysin treated m smegmatis/product/Millipore
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mutanolysin treated m smegmatis - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    87
    Bio-Rad m smegmatis
    Growth of M. <t>smegmatis</t> containing pMindGFP, pMind6938GFP and pMind6939GFP under (a) nutrient-rich, (c) carbon-limitation and (e) nitrogen-limitation conditions as measured by optical density at 590 nm (OD 590 ). Viability of M. smegmatis containing pMindGFP, pMind6938GFP and pMind6939GFP under (b) nutrient-rich, (d) carbon limitation and (f) nitrogen limitation conditions as determined by cfu counts. Each growth assay was performed in 96-well plates in a total volume of 100 µL with an initial optical density at 590 nm (OD 590 ) of 0.01 (equivalent to an OD 600 of approximately 0.05 in a 10 mm path length cuvette). Cultures were incubated at 37°C in 96-well plates and cfu measurements were performed on the cultures after entry into stationary phase: 72 h for carbon- and nitrogen-limited conditions and 144 h for nutrient-rich conditions. Two independent experiments were conducted in quintuplicate yielding similar results. The results of one of these independent experiments are shown. Mean values ± standard error are illustrated.
    M Smegmatis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Bio-Rad
    Average 87 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    92
    Becton Dickinson m smegmatis
    a Fluorescence enhancement and b color changes of 1 mM probe 1 incubated with S. aureus, K. aerogenes, M. avium and M. <t>smegmatis</t> colonies and lysates
    M Smegmatis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Becton Dickinson
    Average 92 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    91
    Formedium m smegmatis
    a Fluorescence enhancement and b color changes of 1 mM probe 1 incubated with S. aureus, K. aerogenes, M. avium and M. <t>smegmatis</t> colonies and lysates
    M Smegmatis, supplied by Formedium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Formedium
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    91
    GenScript m smegmatis
    Rifampicin phenotypic resistance from mistranslation is due to variation in the protein target. ( A ) The RRDR of the β-subunit of RNAP of M. <t>smegmatis</t> . The highlighted residues refer to a glutamine or asparagine residue, that if substituted for
    M Smegmatis, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/GenScript
    Average 91 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    80
    Ciba Geigy m smegmatis
    Rifampicin phenotypic resistance from mistranslation is due to variation in the protein target. ( A ) The RRDR of the β-subunit of RNAP of M. <t>smegmatis</t> . The highlighted residues refer to a glutamine or asparagine residue, that if substituted for
    M Smegmatis, supplied by Ciba Geigy, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Ciba Geigy
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

    84
    Clinical and Laboratory Standards Institute m smegmatis
    Rifampicin phenotypic resistance from mistranslation is due to variation in the protein target. ( A ) The RRDR of the β-subunit of RNAP of M. <t>smegmatis</t> . The highlighted residues refer to a glutamine or asparagine residue, that if substituted for
    M Smegmatis, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Clinical and Laboratory Standards Institute
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    85
    Glaxo Smith m smegmatis
    Expression analysis of the M. <t>smegmatis</t> cyd gene cluster. (A) Genetic organization at the cyd locus of the reporter strain, mc 2 155::pBK4. The hatched arrow denotes the putative cyd promoter, P cyd . The genotype of this site-specific single recombinant was confirmed by Southern blotting (not shown). (B) Dependence of β-galactosidase reporter activity on pO 2 . The specific activity of a reporter gene product (β-galactosidase) was studied at 21, 5, 1, and 0.5% air saturation as described in Materials and Methods. The data shown are an average of three independent experiments for each air saturation level. A statistical pairwise comparison showed that the data followed a significant second-order relationship, as represented in the figure ( P
    M Smegmatis, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Glaxo Smith
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    89
    Janssen m smegmatis
    Real-time measurements of single-cell ATP using genetically encoded biosensors. Wild-type M. <t>smegmatis</t> expressing ATeam biosensors with high (WT_HA), medium (WT_MA), or low (WT_LA) affinity for ATP was used. Cells were grown in minimal-acetate medium in a microfluidic device with addition of bedaquiline (5 µg/ml) at time zero. Phase-contrast and fluorescence images were recorded at 10-min intervals. See Movie S1 in the supplemental material. (A) WT_MA cells. Representative image series recorded on the YFP (top) or FRET (middle) channel. Ratiometric FRET/YFP images (bottom) are pseudocolored. Numbers indicate time elapsed. Scale bar, 5 µm. (B) WT_MA cells. Representative images were recorded immediately before (–BDQ) or 1 h after (+BDQ) addition of bedaquiline to the flow medium. Images are zoomed from the boxed area in panel A. (Left) Phase-contrast image; (middle) merged images recorded on FRET (green) and YFP (red) channels; (right) pseudocolored ratiometric FRET/YFP images. Scale bar, 3 µm. (C) WT_MA cells. Time traces of fluorescence intensities of two representative cells, recorded on FRET (green lines) and YFP (red lines) channels, are shown. The vertical line represents time of bedaquiline addition. (D) Time traces of FRET/YFP ratios of WT_HA (orange), WT_MA (blue), and WT_LA (black) reporter strains. Ranges of the FRET/YFP ratios corresponding to high-ATP and low-ATP states are highlighted in grey.
    M Smegmatis, supplied by Janssen, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/Janssen
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    86
    GE Healthcare m smegmatis
    TLC-autoradiography of FAMEs and MAMEs from M. <t>smegmatis</t> strains overexpressing Mt-KasA, Mt-KasB and Mt-FabH following platensimycin treatment. Platensimycin (0–60 µg/ml) was titred into M. smegmatis cultures at an OD 600 nm of 0.4 prior to labelling with 1 µCi/ml [1,2- 14 C]acetate for 12 h. [ 14 C]-FAMEs and MAMEs were extracted and resolved by TLC. An equivalent aliquot of the resulting solution of FAMEs and MAMEs was subjected to TLC using silica gel plates developed twice in petroleum ether-acetone (95∶5). Autoradiograms were produced by overnight exposure to Kodak X-Omat film to reveal [ 14 C]labeled FAMEs and MAMEs. (A) M. smegmatis pVV16, (B) M. smegmatis pVV16-Mt-KasA, (C) M. smegmatis pVV16-Mt-KasB, and (D) M. smegmatis pVV16-Mt-KasAB.
    M Smegmatis, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/GE Healthcare
    Average 86 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    86
    InvivoGen m smegmatis
    TLC-autoradiography of FAMEs and MAMEs from M. <t>smegmatis</t> strains overexpressing Mt-KasA, Mt-KasB and Mt-FabH following platensimycin treatment. Platensimycin (0–60 µg/ml) was titred into M. smegmatis cultures at an OD 600 nm of 0.4 prior to labelling with 1 µCi/ml [1,2- 14 C]acetate for 12 h. [ 14 C]-FAMEs and MAMEs were extracted and resolved by TLC. An equivalent aliquot of the resulting solution of FAMEs and MAMEs was subjected to TLC using silica gel plates developed twice in petroleum ether-acetone (95∶5). Autoradiograms were produced by overnight exposure to Kodak X-Omat film to reveal [ 14 C]labeled FAMEs and MAMEs. (A) M. smegmatis pVV16, (B) M. smegmatis pVV16-Mt-KasA, (C) M. smegmatis pVV16-Mt-KasB, and (D) M. smegmatis pVV16-Mt-KasAB.
    M Smegmatis, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/InvivoGen
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    92
    MYCO Medical m smegmatis
    Phosphorylation status and activity of FadD32 produced in mycobacteria. The FadD32 protein was produced either in E. coli (non-phosphorylated, FadD32) or in M. <t>smegmatis</t> (FadD32_myco). A, scatter plots showing maximum velocity V max for lauric acid (C
    M Smegmatis, supplied by MYCO Medical, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis/product/MYCO Medical
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m smegmatis - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    84
    MiddleBrook Pharmaceuticals fast growing m smegmatis host
    Interpretation of the MBA results with a lawn of fast-growing M. <t>smegmatis</t> . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain
    Fast Growing M Smegmatis Host, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast growing m smegmatis host/product/MiddleBrook Pharmaceuticals
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fast growing m smegmatis host - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    94
    GenScript m smegmatis fgd
    Interpretation of the MBA results with a lawn of fast-growing M. <t>smegmatis</t> . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain
    M Smegmatis Fgd, supplied by GenScript, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis fgd/product/GenScript
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m smegmatis fgd - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    92
    Agilent technologies m smegmatis microarray
    Interpretation of the MBA results with a lawn of fast-growing M. <t>smegmatis</t> . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain
    M Smegmatis Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis microarray/product/Agilent technologies
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m smegmatis microarray - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    99
    ATCC m smegmatis strains m smegmatis mc2 155
    Delineation of regulatory region . Deletion constructs were generated to segregate promoter and the regulatory regions of IGPr. The column labeled as construct shows configuration of the inserts in different clones used in transformation of <t>M.smegmatis</t> mc2 155 . The numbering is with reference to the translational initiation signal for Rv0167 as +1. The mutation in VPCI591 is shown as a filled triangle, the regions deleted in each clone is indicated by delta symbol. IGPr: 200 bp intergenic region between Rv0166 and Rv0167.
    M Smegmatis Strains M Smegmatis Mc2 155, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis strains m smegmatis mc2 155/product/ATCC
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    m smegmatis strains m smegmatis mc2 155 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    86
    MiddleBrook Pharmaceuticals m smegmatis mc2 155
    Delineation of regulatory region . Deletion constructs were generated to segregate promoter and the regulatory regions of IGPr. The column labeled as construct shows configuration of the inserts in different clones used in transformation of <t>M.smegmatis</t> mc2 155 . The numbering is with reference to the translational initiation signal for Rv0167 as +1. The mutation in VPCI591 is shown as a filled triangle, the regions deleted in each clone is indicated by delta symbol. IGPr: 200 bp intergenic region between Rv0166 and Rv0167.
    M Smegmatis Mc2 155, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis mc2 155/product/MiddleBrook Pharmaceuticals
    Average 86 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    m smegmatis mc2 155 - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    92
    MiddleBrook Pharmaceuticals wild type m smegmatis
    Delineation of regulatory region . Deletion constructs were generated to segregate promoter and the regulatory regions of IGPr. The column labeled as construct shows configuration of the inserts in different clones used in transformation of <t>M.smegmatis</t> mc2 155 . The numbering is with reference to the translational initiation signal for Rv0167 as +1. The mutation in VPCI591 is shown as a filled triangle, the regions deleted in each clone is indicated by delta symbol. IGPr: 200 bp intergenic region between Rv0166 and Rv0167.
    Wild Type M Smegmatis, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type m smegmatis/product/MiddleBrook Pharmaceuticals
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    wild type m smegmatis - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    85
    Cosmo Bio m smegmatis strains
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    M Smegmatis Strains, supplied by Cosmo Bio, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis strains/product/Cosmo Bio
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m smegmatis strains - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    87
    Bio-Rad m smegmatis mc2 155
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    M Smegmatis Mc2 155, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis mc2 155/product/Bio-Rad
    Average 87 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    m smegmatis mc2 155 - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    91
    Eppendorf AG m smegmatis mc2 155
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    M Smegmatis Mc2 155, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis mc2 155/product/Eppendorf AG
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m smegmatis mc2 155 - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    91
    Difco m smegmatis mc2 155
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    M Smegmatis Mc2 155, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis mc2 155/product/Difco
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m smegmatis mc2 155 - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    84
    Genentech m smegmatis cultures
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    M Smegmatis Cultures, supplied by Genentech, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis cultures/product/Genentech
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m smegmatis cultures - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    94
    Janssen screen against m smegmatis
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    Screen Against M Smegmatis, supplied by Janssen, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/screen against m smegmatis/product/Janssen
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    screen against m smegmatis - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    DSMZ culture conditions m smegmatis mc2 155
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    Culture Conditions M Smegmatis Mc2 155, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/culture conditions m smegmatis mc2 155/product/DSMZ
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    culture conditions m smegmatis mc2 155 - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    85
    Bio-Rad m smegmatis competent cells
    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. <t>smegmatis</t> mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and
    M Smegmatis Competent Cells, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m smegmatis competent cells/product/Bio-Rad
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m smegmatis competent cells - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Southern analysis with an erm (38)-specific probe. Lanes: S1, M. smegmatis mc 2 155; S2, M. smegmatis ATCC 14468; F, M. fortuitum ATCC 6841; A, M. abscessus MAB30; C, M. chelonae ATCC 35752; M, M. microti ATCC 19422; B, M. bovis BCG.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Intrinsic Macrolide Resistance in Mycobacterium smegmatis Is Conferred by a Novel erm Gene, erm(38)

    doi: 10.1128/AAC.47.10.3053-3060.2003

    Figure Lengend Snippet: Southern analysis with an erm (38)-specific probe. Lanes: S1, M. smegmatis mc 2 155; S2, M. smegmatis ATCC 14468; F, M. fortuitum ATCC 6841; A, M. abscessus MAB30; C, M. chelonae ATCC 35752; M, M. microti ATCC 19422; B, M. bovis BCG.

    Article Snippet: In contrast to the other mycobacteria, M. fortuitum (strain ATCC 6841 and 2 clinical isolates), M. microti ATCC 19422, and M. smegmatis (strains mc2 155 and ATCC 14468) cultures preincubated with macrolide all presented with a MIC of clarithromycin which was > 32-fold higher than that of controls.

    Techniques:

    Relationship between birth length and other single cell measures for single M. smegmatis cells grown with glycerol as a sole carbon source. Red circles, single cell measurements; histograms, distribution of individual cells, with kernel density estimates depicted as a blue line; solid line with white filled circles, mean of birth length bins with error bars denoting the 95% confidence interval of the estimate determined by bootstrapping; gray dotted line, the least squares linear regression fit of unbinned data; Key, slope —the slope of the regression line (± the 95% confidence interval), intercept —the y -intercept, r —the Pearson correlation coefficient, n —the total number of cells measured, (A) Division length is plotted against birth length. (B) Interdivision time against birth length. (C) Added length against exponential growth rate. (D) Exponential growth rate against birth length.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Mycobacteria Modify Their Cell Size Control under Sub-Optimal Carbon Sources

    doi: 10.3389/fcell.2017.00064

    Figure Lengend Snippet: Relationship between birth length and other single cell measures for single M. smegmatis cells grown with glycerol as a sole carbon source. Red circles, single cell measurements; histograms, distribution of individual cells, with kernel density estimates depicted as a blue line; solid line with white filled circles, mean of birth length bins with error bars denoting the 95% confidence interval of the estimate determined by bootstrapping; gray dotted line, the least squares linear regression fit of unbinned data; Key, slope —the slope of the regression line (± the 95% confidence interval), intercept —the y -intercept, r —the Pearson correlation coefficient, n —the total number of cells measured, (A) Division length is plotted against birth length. (B) Interdivision time against birth length. (C) Added length against exponential growth rate. (D) Exponential growth rate against birth length.

    Article Snippet: We tested whether this holds for M. smegmatis grown in glycerol, pyruvate, and acetate, given that, at least between pyruvate and glycerol/acetate, the mean population growth rate is altered (Table ).

    Techniques:

    The effect of standard antimycobacterial agents on the growth of M. smegmatis . M. smegmatis mc 2 155 was treated with different concentrations of isoniazid ( a ) and kanamycin ( b ) from 0 μg/ml to 100 μg/ml. The bacteria were incubated with drugs at 37 ˚C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts

    doi: 10.1186/s12906-017-1801-5

    Figure Lengend Snippet: The effect of standard antimycobacterial agents on the growth of M. smegmatis . M. smegmatis mc 2 155 was treated with different concentrations of isoniazid ( a ) and kanamycin ( b ) from 0 μg/ml to 100 μg/ml. The bacteria were incubated with drugs at 37 ˚C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm

    Article Snippet: Growth of M. smegmatis M. smegmatis was grown in Middlebrook 7H9 media (5.2 g/L) supplemented with 1 g/L casein acid hydrolysate.

    Techniques: Incubation, MTT Assay

    The effect of P. curatellifolia acetone and ethanol extracts on the growth of M. smegmatis . M. smegmatis mc 2 155 laboratory strain at concentration of 2 × 10 6 cfu/mL was treated with different concentrations of the acetone ( a ) and ethanol extract ( b ) from 0 μg/ml up to 100 μg/ml. The bacteria were incubated with extracts at 37 ˚ C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm. The error bars on the graphs indicate the standard deviation from mean ( n = 4). Dunnet’s multiple comparison test was used to compare columns using Graphpad prism 6 software. The asterisks (*) indicate statistical significant differences with the sterility control (media) *

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts

    doi: 10.1186/s12906-017-1801-5

    Figure Lengend Snippet: The effect of P. curatellifolia acetone and ethanol extracts on the growth of M. smegmatis . M. smegmatis mc 2 155 laboratory strain at concentration of 2 × 10 6 cfu/mL was treated with different concentrations of the acetone ( a ) and ethanol extract ( b ) from 0 μg/ml up to 100 μg/ml. The bacteria were incubated with extracts at 37 ˚ C for 20 h and cell density was determined by measuring absorbance of reduced MTT at 590 nm. The error bars on the graphs indicate the standard deviation from mean ( n = 4). Dunnet’s multiple comparison test was used to compare columns using Graphpad prism 6 software. The asterisks (*) indicate statistical significant differences with the sterility control (media) *

    Article Snippet: Growth of M. smegmatis M. smegmatis was grown in Middlebrook 7H9 media (5.2 g/L) supplemented with 1 g/L casein acid hydrolysate.

    Techniques: Concentration Assay, Incubation, MTT Assay, Standard Deviation, Software, Sterility

    Genome analysis reveals 28 putative carbohydrate uptake systems in M. smegmatis .

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Genome analysis reveals 28 putative carbohydrate uptake systems in M. smegmatis .

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques:

    Sugar transport systems of M. smegmatis and M. tuberculosis . Shown are the permeases of the ABC, PTS, MIP, MFS, and SSS families. The derived putative substrates are inferred from in silico analyses in combination with experimental data.

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Sugar transport systems of M. smegmatis and M. tuberculosis . Shown are the permeases of the ABC, PTS, MIP, MFS, and SSS families. The derived putative substrates are inferred from in silico analyses in combination with experimental data.

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques: Derivative Assay, In Silico

    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques:

    Growth of M. smegmatis on carbohydrates as single carbon sources.

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Growth of M. smegmatis on carbohydrates as single carbon sources.

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques:

    Genetic organization of M. smegmatis carbohydrate transporters of the ABC family. The arrows indicate the lengths and transcriptional orientations of annotated genes and predicted ORFs. Genes encoding transport systems are depicted in dark gray, carbohydrate

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Genetic organization of M. smegmatis carbohydrate transporters of the ABC family. The arrows indicate the lengths and transcriptional orientations of annotated genes and predicted ORFs. Genes encoding transport systems are depicted in dark gray, carbohydrate

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques:

    Genetic organization of M. smegmatis carbohydrate transporters of the PTS, MIP, SSS, and MFS protein families. For an explanation of the data, see the legend to Fig. .

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Genetic organization of M. smegmatis carbohydrate transporters of the PTS, MIP, SSS, and MFS protein families. For an explanation of the data, see the legend to Fig. .

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques:

    Growth of M. smegmatis in minimal medium with different carbon sources. The growth of M. smegmatis mc 2 155 at 37°C in HB medium containing 1% glucose (circles), 1% trehalose (diamonds), 1% maltose (squares), and 1%

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Growth of M. smegmatis in minimal medium with different carbon sources. The growth of M. smegmatis mc 2 155 at 37°C in HB medium containing 1% glucose (circles), 1% trehalose (diamonds), 1% maltose (squares), and 1%

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques:

    Expression analysis of selected genes of M. smegmatis . The figure shows 1% agarose gels with PCR products from semiquantitative RT-PCR experiments. For each gene, samples were taken periodically along the PCR. The depicted bands show products

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Expression analysis of selected genes of M. smegmatis . The figure shows 1% agarose gels with PCR products from semiquantitative RT-PCR experiments. For each gene, samples were taken periodically along the PCR. The depicted bands show products

    Article Snippet: This demonstrates that fructose uptake is inducible in M. smegmatis .

    Techniques: Expressing, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    Interpretation of the MBA results with a lawn of fast-growing M. smegmatis . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain

    Journal: Journal of Clinical Microbiology

    Article Title: Use of a Mycobacteriophage-Based Assay for Rapid Assessment of Susceptibilities of Mycobacterium tuberculosis Isolates to Isoniazid and Influence of Resistance Level on Assay Performance

    doi: 10.1128/JCM.44.1.201-205.2006

    Figure Lengend Snippet: Interpretation of the MBA results with a lawn of fast-growing M. smegmatis . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain

    Article Snippet: The mycobacteriophage stock was quantified by pipetting 10-μl aliquots of serial dilutions onto a lawn of M. smegmatis growth.

    Techniques: Incubation

    Induction of antibody responses with recombinant M. smegmatis is limited by the amount of HIV-1 Env expressed in recombinant M. smegmatis pJH154-gp120. To evaluate the immunogenicity of recombinant M. smegmatis expressing CON6 env in mice, mice were immunized once with either killed or live recombinant M. smegmatis expressing CON6 gp120 with or without IFA adjuvant. The geometric mean log endpoint antibody titers (± standard errors of the means; n = 5) are shown on the y axis. Abbreviations: rMsmeg, recombinant M. smegmatis expressing HIV-1 CON6 Env; CON6, recombinant CON6 gp140CF protein.

    Journal: Clinical and Vaccine Immunology

    Article Title: Generation of Mucosal Anti-Human Immunodeficiency Virus Type 1 T-Cell Responses by Recombinant Mycobacterium smegmatis▿

    doi: 10.1128/CVI.00195-06

    Figure Lengend Snippet: Induction of antibody responses with recombinant M. smegmatis is limited by the amount of HIV-1 Env expressed in recombinant M. smegmatis pJH154-gp120. To evaluate the immunogenicity of recombinant M. smegmatis expressing CON6 env in mice, mice were immunized once with either killed or live recombinant M. smegmatis expressing CON6 gp120 with or without IFA adjuvant. The geometric mean log endpoint antibody titers (± standard errors of the means; n = 5) are shown on the y axis. Abbreviations: rMsmeg, recombinant M. smegmatis expressing HIV-1 CON6 Env; CON6, recombinant CON6 gp140CF protein.

    Article Snippet: Figure shows that the expression of the intact CON6 gp120 was detected in recombinant M. smegmatis transformed with either the surface expression plasmid pJH152 (Fig. , lane 4), the intracellular expression plasmid pJH153 (lane 5), or the secreted expression plasmid pJH154 (lane 6).

    Techniques: Recombinant, Expressing, Mouse Assay, Immunofluorescence

    HIV-1 envelope-specific and vector-specific T-cell responses in the spleen, lungs, and FRT of mice immunized with recombinant M. smegmatis . HIV-1-specific (A to C) and vector-specific (D to F) T-cell responses in the spleen (A and D), lungs (B and E), and FRT (C and F) were assessed by IFN-γ ELISPOT assays. Mice that were primed with rAd-CON6 and boosted with rVV-CON6 were used as positive controls. Unimmunized mice were used as negative controls. Empty bars represent immunization groups that received prime/boost immunizations. Solid bars represent immunization groups that received priming immunization only. Mean (± standard error of the mean) numbers of SFC per 10 6 cells are shown on the y axis. The indicated immunization groups and doses are shown on the x axis. Assays were performed and analyzed with pooled lung and FRT samples as described in Materials and Methods.

    Journal: Clinical and Vaccine Immunology

    Article Title: Generation of Mucosal Anti-Human Immunodeficiency Virus Type 1 T-Cell Responses by Recombinant Mycobacterium smegmatis▿

    doi: 10.1128/CVI.00195-06

    Figure Lengend Snippet: HIV-1 envelope-specific and vector-specific T-cell responses in the spleen, lungs, and FRT of mice immunized with recombinant M. smegmatis . HIV-1-specific (A to C) and vector-specific (D to F) T-cell responses in the spleen (A and D), lungs (B and E), and FRT (C and F) were assessed by IFN-γ ELISPOT assays. Mice that were primed with rAd-CON6 and boosted with rVV-CON6 were used as positive controls. Unimmunized mice were used as negative controls. Empty bars represent immunization groups that received prime/boost immunizations. Solid bars represent immunization groups that received priming immunization only. Mean (± standard error of the mean) numbers of SFC per 10 6 cells are shown on the y axis. The indicated immunization groups and doses are shown on the x axis. Assays were performed and analyzed with pooled lung and FRT samples as described in Materials and Methods.

    Article Snippet: Figure shows that the expression of the intact CON6 gp120 was detected in recombinant M. smegmatis transformed with either the surface expression plasmid pJH152 (Fig. , lane 4), the intracellular expression plasmid pJH153 (lane 5), or the secreted expression plasmid pJH154 (lane 6).

    Techniques: Plasmid Preparation, Mouse Assay, Recombinant, Enzyme-linked Immunospot

    Expression of HIV-1 CON6 gp120 and gp140CF envelope proteins in M. smegmatis . (A) Western blot analysis of CON6 Env protein expression in various M. smegmatis designs, including molecular weight (Mr.) standards (lane 1), untransformed M. smegmatis (lane 2), and recombinant M. smegmatis transformed with either empty plasmid (lane 3), the surface expression plasmid pJH152-CON6 gp120 (lane 4), the intracellular expression plasmid pJH153-CON6 gp120 (lane 5), the secreted expression plasmid pJH154-CON6 gp120 (lane 6), or the surface expression plasmid pJH152-CON6 gp140 (lane 7) or pJH222-CON6 gp140 (lane 8). Both the intact (89 kDa for gp140, 79 kDa and 74 kDa for gp120) and partially cleaved (49 kDa and 34 kDa) CON6 Env protein products, as indicated by arrows, were found with gp120 MAb T8 (anti-C1 gp120 region), gp41-specific MAb 7B2, and V3 loop-specific MAb 7B9. (B) Schematic representation of the full-length (top) and cleaved (bottom) gp140, indicating the binding locations of MAbs T8, 7B2, and 7B9. Full-length gp140 could be detected by T8, 7B2, or 7B9, while the cleaved 34-kDa Env protein band could be detected only by MAb T8. A 50-kDa protein band could be detected by both MAbs 7B2 and 7B9. (C) Western blot analysis of supernatants from untransformed M. smegmatis (lane 1) and recombinant M. smegmatis transformed with the empty plasmid (lane 2) or pJH154-CON6gp140CF (lane 3) for detecting secreted CON6 Env protein expression. A truncated gp140 protein (lane 3) could be detected by 7B9, as indicated by the arrow. A high-molecular-weight protein band, as indicated by an arrowhead, was nonspecific, as it was also detected in recombinant M. smegmatis transformed with pJH154-CON6gp140CF (lane 3) and in the negative control lanes 1 and 2.

    Journal: Clinical and Vaccine Immunology

    Article Title: Generation of Mucosal Anti-Human Immunodeficiency Virus Type 1 T-Cell Responses by Recombinant Mycobacterium smegmatis▿

    doi: 10.1128/CVI.00195-06

    Figure Lengend Snippet: Expression of HIV-1 CON6 gp120 and gp140CF envelope proteins in M. smegmatis . (A) Western blot analysis of CON6 Env protein expression in various M. smegmatis designs, including molecular weight (Mr.) standards (lane 1), untransformed M. smegmatis (lane 2), and recombinant M. smegmatis transformed with either empty plasmid (lane 3), the surface expression plasmid pJH152-CON6 gp120 (lane 4), the intracellular expression plasmid pJH153-CON6 gp120 (lane 5), the secreted expression plasmid pJH154-CON6 gp120 (lane 6), or the surface expression plasmid pJH152-CON6 gp140 (lane 7) or pJH222-CON6 gp140 (lane 8). Both the intact (89 kDa for gp140, 79 kDa and 74 kDa for gp120) and partially cleaved (49 kDa and 34 kDa) CON6 Env protein products, as indicated by arrows, were found with gp120 MAb T8 (anti-C1 gp120 region), gp41-specific MAb 7B2, and V3 loop-specific MAb 7B9. (B) Schematic representation of the full-length (top) and cleaved (bottom) gp140, indicating the binding locations of MAbs T8, 7B2, and 7B9. Full-length gp140 could be detected by T8, 7B2, or 7B9, while the cleaved 34-kDa Env protein band could be detected only by MAb T8. A 50-kDa protein band could be detected by both MAbs 7B2 and 7B9. (C) Western blot analysis of supernatants from untransformed M. smegmatis (lane 1) and recombinant M. smegmatis transformed with the empty plasmid (lane 2) or pJH154-CON6gp140CF (lane 3) for detecting secreted CON6 Env protein expression. A truncated gp140 protein (lane 3) could be detected by 7B9, as indicated by the arrow. A high-molecular-weight protein band, as indicated by an arrowhead, was nonspecific, as it was also detected in recombinant M. smegmatis transformed with pJH154-CON6gp140CF (lane 3) and in the negative control lanes 1 and 2.

    Article Snippet: Figure shows that the expression of the intact CON6 gp120 was detected in recombinant M. smegmatis transformed with either the surface expression plasmid pJH152 (Fig. , lane 4), the intracellular expression plasmid pJH153 (lane 5), or the secreted expression plasmid pJH154 (lane 6).

    Techniques: Expressing, Western Blot, Molecular Weight, Recombinant, Transformation Assay, Plasmid Preparation, Binding Assay, Negative Control

    Point mutation in the cbs1 allele of the sud mutant PM440. Shown is the sense-strand DNA sequence in the region near the start codon of the cbs gene of M. smegmatis . Upstream is the 3′ end of the lipU gene, followed by a 29-nucleotide intergenic

    Journal:

    Article Title: An Unusual Mutation Results in the Replacement of Diaminopimelate with Lanthionine in the Peptidoglycan of a Mutant Strain of Mycobacterium smegmatis †

    doi: 10.1128/JB.187.5.1612-1620.2005

    Figure Lengend Snippet: Point mutation in the cbs1 allele of the sud mutant PM440. Shown is the sense-strand DNA sequence in the region near the start codon of the cbs gene of M. smegmatis . Upstream is the 3′ end of the lipU gene, followed by a 29-nucleotide intergenic

    Article Snippet: Sucrose was added to media at a concentration of 2% (wt/vol) when required. l- Lysine, l- methionine, and l- threonine were added at 40 μg/ml to all M. smegmatis cultures in Middlebrook media, dl -homoserine was used at 80 μg/ml, and meso -diaminopimelate was added at 200 μg/ml to both LB broth with 0.05% Tween 80 and Middlebrook media.

    Techniques: Mutagenesis, Sequencing

    Growth of M. smegmatis containing pMindGFP, pMind6938GFP and pMind6939GFP under (a) nutrient-rich, (c) carbon-limitation and (e) nitrogen-limitation conditions as measured by optical density at 590 nm (OD 590 ). Viability of M. smegmatis containing pMindGFP, pMind6938GFP and pMind6939GFP under (b) nutrient-rich, (d) carbon limitation and (f) nitrogen limitation conditions as determined by cfu counts. Each growth assay was performed in 96-well plates in a total volume of 100 µL with an initial optical density at 590 nm (OD 590 ) of 0.01 (equivalent to an OD 600 of approximately 0.05 in a 10 mm path length cuvette). Cultures were incubated at 37°C in 96-well plates and cfu measurements were performed on the cultures after entry into stationary phase: 72 h for carbon- and nitrogen-limited conditions and 144 h for nutrient-rich conditions. Two independent experiments were conducted in quintuplicate yielding similar results. The results of one of these independent experiments are shown. Mean values ± standard error are illustrated.

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Targeting the chromosome partitioning protein ParA in tuberculosis drug discovery

    doi: 10.1093/jac/dkq311

    Figure Lengend Snippet: Growth of M. smegmatis containing pMindGFP, pMind6938GFP and pMind6939GFP under (a) nutrient-rich, (c) carbon-limitation and (e) nitrogen-limitation conditions as measured by optical density at 590 nm (OD 590 ). Viability of M. smegmatis containing pMindGFP, pMind6938GFP and pMind6939GFP under (b) nutrient-rich, (d) carbon limitation and (f) nitrogen limitation conditions as determined by cfu counts. Each growth assay was performed in 96-well plates in a total volume of 100 µL with an initial optical density at 590 nm (OD 590 ) of 0.01 (equivalent to an OD 600 of approximately 0.05 in a 10 mm path length cuvette). Cultures were incubated at 37°C in 96-well plates and cfu measurements were performed on the cultures after entry into stationary phase: 72 h for carbon- and nitrogen-limited conditions and 144 h for nutrient-rich conditions. Two independent experiments were conducted in quintuplicate yielding similar results. The results of one of these independent experiments are shown. Mean values ± standard error are illustrated.

    Article Snippet: Induction of parA antisense in M. smegmatis resulted in a minor but statistically significant increase in cell length (5.08 ± 1.45 to 6.8 ± 1.3 µm).

    Techniques: Growth Assay, Incubation

    (a) Growth of M. smegmatis on agar at 72 h following electroporation with (i) pMindGFP, (ii) pMind6938GFP, (iii) pMind6939GFP and (iv) pMind6938 + 6939GFP. Electroporations were performed in triplicate in two independent experiments, yielding similar results. The results of one of these independent experiments are shown. (b) Induction of tetO operator by addition of 0.02 mg/L tetracycline (TET) as measured by GFP fluorescence. RFU, relative fluorescence units. Results are mean values of triplicates ± standard error.

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Targeting the chromosome partitioning protein ParA in tuberculosis drug discovery

    doi: 10.1093/jac/dkq311

    Figure Lengend Snippet: (a) Growth of M. smegmatis on agar at 72 h following electroporation with (i) pMindGFP, (ii) pMind6938GFP, (iii) pMind6939GFP and (iv) pMind6938 + 6939GFP. Electroporations were performed in triplicate in two independent experiments, yielding similar results. The results of one of these independent experiments are shown. (b) Induction of tetO operator by addition of 0.02 mg/L tetracycline (TET) as measured by GFP fluorescence. RFU, relative fluorescence units. Results are mean values of triplicates ± standard error.

    Article Snippet: Induction of parA antisense in M. smegmatis resulted in a minor but statistically significant increase in cell length (5.08 ± 1.45 to 6.8 ± 1.3 µm).

    Techniques: Electroporation, Fluorescence

    Restriction map and genetic organization of the part of exochelin gene cluster of M. smegmatis mc 2 155 relevant to the present study. The arrows indicate the direction of gene transcription. Boxes represent chromosomal fragments cloned in pGS22 (fragments B and C) and pGS31 (fragments A and B). Fragment A is the 507-bp part upstream of fxbA gene, fragment B (758 bp) contains the entire fxuB gene, and fragment C (768 bp) contains a part of the fxuC gene.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Screening System for Xenosiderophores as Potential Drug Delivery Agents in Mycobacteria

    doi: 10.1128/AAC.45.5.1317-1322.2001

    Figure Lengend Snippet: Restriction map and genetic organization of the part of exochelin gene cluster of M. smegmatis mc 2 155 relevant to the present study. The arrows indicate the direction of gene transcription. Boxes represent chromosomal fragments cloned in pGS22 (fragments B and C) and pGS31 (fragments A and B). Fragment A is the 507-bp part upstream of fxbA gene, fragment B (758 bp) contains the entire fxuB gene, and fragment C (768 bp) contains a part of the fxuC gene.

    Article Snippet: Plasmids were isolated with a QIAprep Spin kit (Qiagen) and transformed into E. coli strains by standard techniques or electroporated into M. smegmatis strains with a Gene Pulser (Bio-Rad Laboratories) according to the manufacture protocol, with modifications for electroporation into M. smegmatis used as described before ( ).

    Techniques: Clone Assay

    Replacement of the fxbA gene in M. smegmatis mc 2 155. (A) Strategy used to replace the fxbA gene by double crossover. The horizontal arrows indicate annealing sites of primers used to confirm the replacement (B1-K1, B9-K2) by using the chromosomal DNA of mutant strain B1 as the template and to generate control fragments by using plamid pGS31 as the template. The expected sizes of the fragments are indicated between the arrows. (B) Confirmation of replacement of the fxbA gene by PCR analysis of DNA from the B3 Δ fxbA mutant.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Screening System for Xenosiderophores as Potential Drug Delivery Agents in Mycobacteria

    doi: 10.1128/AAC.45.5.1317-1322.2001

    Figure Lengend Snippet: Replacement of the fxbA gene in M. smegmatis mc 2 155. (A) Strategy used to replace the fxbA gene by double crossover. The horizontal arrows indicate annealing sites of primers used to confirm the replacement (B1-K1, B9-K2) by using the chromosomal DNA of mutant strain B1 as the template and to generate control fragments by using plamid pGS31 as the template. The expected sizes of the fragments are indicated between the arrows. (B) Confirmation of replacement of the fxbA gene by PCR analysis of DNA from the B3 Δ fxbA mutant.

    Article Snippet: Plasmids were isolated with a QIAprep Spin kit (Qiagen) and transformed into E. coli strains by standard techniques or electroporated into M. smegmatis strains with a Gene Pulser (Bio-Rad Laboratories) according to the manufacture protocol, with modifications for electroporation into M. smegmatis used as described before ( ).

    Techniques: Mutagenesis, Polymerase Chain Reaction

    Replacement of the fxuA gene in M. smegmatis mc 2 155 mutant strain M24. (A) Strategy used to replace the fxuA gene by double crossover. The horizontal arrows indicate annealing sites of primers used to confirm the replacement (U3-K1, U9-K2) by using the chromosomal DNA of mutant strain U3 as the template and to generate control fragments by using plasmid pGS22 as the template. The expected sizes of the fragments are indicated between the arrows. (B) Confirmation of replacement of the fxuA gene by PCR analysis of DNA from the U3 Δ fxuA mutant.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Screening System for Xenosiderophores as Potential Drug Delivery Agents in Mycobacteria

    doi: 10.1128/AAC.45.5.1317-1322.2001

    Figure Lengend Snippet: Replacement of the fxuA gene in M. smegmatis mc 2 155 mutant strain M24. (A) Strategy used to replace the fxuA gene by double crossover. The horizontal arrows indicate annealing sites of primers used to confirm the replacement (U3-K1, U9-K2) by using the chromosomal DNA of mutant strain U3 as the template and to generate control fragments by using plasmid pGS22 as the template. The expected sizes of the fragments are indicated between the arrows. (B) Confirmation of replacement of the fxuA gene by PCR analysis of DNA from the U3 Δ fxuA mutant.

    Article Snippet: Plasmids were isolated with a QIAprep Spin kit (Qiagen) and transformed into E. coli strains by standard techniques or electroporated into M. smegmatis strains with a Gene Pulser (Bio-Rad Laboratories) according to the manufacture protocol, with modifications for electroporation into M. smegmatis used as described before ( ).

    Techniques: Mutagenesis, Plasmid Preparation, Polymerase Chain Reaction

    a Fluorescence enhancement and b color changes of 1 mM probe 1 incubated with S. aureus, K. aerogenes, M. avium and M. smegmatis colonies and lysates

    Journal: AMB Express

    Article Title: Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays

    doi: 10.1186/s13568-017-0449-3

    Figure Lengend Snippet: a Fluorescence enhancement and b color changes of 1 mM probe 1 incubated with S. aureus, K. aerogenes, M. avium and M. smegmatis colonies and lysates

    Article Snippet: Fluorescence enhancement of probe 1 with K. aerogenes , M. avium and M. smegmatis (Fig. a; Additional file : Figure S4) indicated that the bacterial colonies and lysates having sulfatase activity hydrolyzed the sulfate ester of probe 1 to generate fluorescent N -methyl isoindole.

    Techniques: Fluorescence, Incubation

    Rifampicin phenotypic resistance from mistranslation is due to variation in the protein target. ( A ) The RRDR of the β-subunit of RNAP of M. smegmatis . The highlighted residues refer to a glutamine or asparagine residue, that if substituted for

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Mycobacterial mistranslation is necessary and sufficient for rifampicin phenotypic resistance

    doi: 10.1073/pnas.1317580111

    Figure Lengend Snippet: Rifampicin phenotypic resistance from mistranslation is due to variation in the protein target. ( A ) The RRDR of the β-subunit of RNAP of M. smegmatis . The highlighted residues refer to a glutamine or asparagine residue, that if substituted for

    Article Snippet: The sequence of the protein fusion of Renilla and firefly luciferase genes was codon-optimized for expression in M. smegmatis and synthesized synthetically (Genscript–GenBank ).

    Techniques:

    Expression analysis of the M. smegmatis cyd gene cluster. (A) Genetic organization at the cyd locus of the reporter strain, mc 2 155::pBK4. The hatched arrow denotes the putative cyd promoter, P cyd . The genotype of this site-specific single recombinant was confirmed by Southern blotting (not shown). (B) Dependence of β-galactosidase reporter activity on pO 2 . The specific activity of a reporter gene product (β-galactosidase) was studied at 21, 5, 1, and 0.5% air saturation as described in Materials and Methods. The data shown are an average of three independent experiments for each air saturation level. A statistical pairwise comparison showed that the data followed a significant second-order relationship, as represented in the figure ( P

    Journal: Journal of Bacteriology

    Article Title: Characterization of the cydAB-Encoded Cytochrome bd Oxidase from Mycobacterium smegmatis

    doi: 10.1128/JB.183.24.7076-7086.2001

    Figure Lengend Snippet: Expression analysis of the M. smegmatis cyd gene cluster. (A) Genetic organization at the cyd locus of the reporter strain, mc 2 155::pBK4. The hatched arrow denotes the putative cyd promoter, P cyd . The genotype of this site-specific single recombinant was confirmed by Southern blotting (not shown). (B) Dependence of β-galactosidase reporter activity on pO 2 . The specific activity of a reporter gene product (β-galactosidase) was studied at 21, 5, 1, and 0.5% air saturation as described in Materials and Methods. The data shown are an average of three independent experiments for each air saturation level. A statistical pairwise comparison showed that the data followed a significant second-order relationship, as represented in the figure ( P

    Article Snippet: A 2.5-kb Sal I fragment carrying the cydAB genes was isolated from the cosmid and used to probe a gridded plasmid library of M. smegmatis (kindly provided by M. Everettt, Glaxo Wellcome, Stevenage, United Kingdom).

    Techniques: Expressing, Recombinant, Southern Blot, Activity Assay

    Proposed aerobic respiratory pathways in M. smegmatis and related organisms. The pathways in B. subtilis . The M. tuberculosis ), and those of M. smegmatis ). Solid arrows denote confirmed pathways, and dashed arrows denote putative pathways. The mycobacterial cytochrome reductase complex (QcrCAB) is denoted as “ bcc ” in accordance with the designation of QcrC as cytochrome cc ).

    Journal: Journal of Bacteriology

    Article Title: Characterization of the cydAB-Encoded Cytochrome bd Oxidase from Mycobacterium smegmatis

    doi: 10.1128/JB.183.24.7076-7086.2001

    Figure Lengend Snippet: Proposed aerobic respiratory pathways in M. smegmatis and related organisms. The pathways in B. subtilis . The M. tuberculosis ), and those of M. smegmatis ). Solid arrows denote confirmed pathways, and dashed arrows denote putative pathways. The mycobacterial cytochrome reductase complex (QcrCAB) is denoted as “ bcc ” in accordance with the designation of QcrC as cytochrome cc ).

    Article Snippet: A 2.5-kb Sal I fragment carrying the cydAB genes was isolated from the cosmid and used to probe a gridded plasmid library of M. smegmatis (kindly provided by M. Everettt, Glaxo Wellcome, Stevenage, United Kingdom).

    Techniques:

    Spectral characterization of membrane fractions isolated from M. smegmatis grown in an oxystat. The spectral profiles of purified membrane fractions from M. smegmatis grown at 21% (A) and 1% (B) air saturation are shown. Reduction was accomplished by the addition of sodium dithionite, and oxidation was accomplished by the addition of potassium ferricyanide.

    Journal: Journal of Bacteriology

    Article Title: Characterization of the cydAB-Encoded Cytochrome bd Oxidase from Mycobacterium smegmatis

    doi: 10.1128/JB.183.24.7076-7086.2001

    Figure Lengend Snippet: Spectral characterization of membrane fractions isolated from M. smegmatis grown in an oxystat. The spectral profiles of purified membrane fractions from M. smegmatis grown at 21% (A) and 1% (B) air saturation are shown. Reduction was accomplished by the addition of sodium dithionite, and oxidation was accomplished by the addition of potassium ferricyanide.

    Article Snippet: A 2.5-kb Sal I fragment carrying the cydAB genes was isolated from the cosmid and used to probe a gridded plasmid library of M. smegmatis (kindly provided by M. Everettt, Glaxo Wellcome, Stevenage, United Kingdom).

    Techniques: Isolation, Purification

    Real-time measurements of single-cell ATP using genetically encoded biosensors. Wild-type M. smegmatis expressing ATeam biosensors with high (WT_HA), medium (WT_MA), or low (WT_LA) affinity for ATP was used. Cells were grown in minimal-acetate medium in a microfluidic device with addition of bedaquiline (5 µg/ml) at time zero. Phase-contrast and fluorescence images were recorded at 10-min intervals. See Movie S1 in the supplemental material. (A) WT_MA cells. Representative image series recorded on the YFP (top) or FRET (middle) channel. Ratiometric FRET/YFP images (bottom) are pseudocolored. Numbers indicate time elapsed. Scale bar, 5 µm. (B) WT_MA cells. Representative images were recorded immediately before (–BDQ) or 1 h after (+BDQ) addition of bedaquiline to the flow medium. Images are zoomed from the boxed area in panel A. (Left) Phase-contrast image; (middle) merged images recorded on FRET (green) and YFP (red) channels; (right) pseudocolored ratiometric FRET/YFP images. Scale bar, 3 µm. (C) WT_MA cells. Time traces of fluorescence intensities of two representative cells, recorded on FRET (green lines) and YFP (red lines) channels, are shown. The vertical line represents time of bedaquiline addition. (D) Time traces of FRET/YFP ratios of WT_HA (orange), WT_MA (blue), and WT_LA (black) reporter strains. Ranges of the FRET/YFP ratios corresponding to high-ATP and low-ATP states are highlighted in grey.

    Journal: mBio

    Article Title: Single-Cell Tracking Reveals Antibiotic-Induced Changes in Mycobacterial Energy Metabolism

    doi: 10.1128/mBio.02236-14

    Figure Lengend Snippet: Real-time measurements of single-cell ATP using genetically encoded biosensors. Wild-type M. smegmatis expressing ATeam biosensors with high (WT_HA), medium (WT_MA), or low (WT_LA) affinity for ATP was used. Cells were grown in minimal-acetate medium in a microfluidic device with addition of bedaquiline (5 µg/ml) at time zero. Phase-contrast and fluorescence images were recorded at 10-min intervals. See Movie S1 in the supplemental material. (A) WT_MA cells. Representative image series recorded on the YFP (top) or FRET (middle) channel. Ratiometric FRET/YFP images (bottom) are pseudocolored. Numbers indicate time elapsed. Scale bar, 5 µm. (B) WT_MA cells. Representative images were recorded immediately before (–BDQ) or 1 h after (+BDQ) addition of bedaquiline to the flow medium. Images are zoomed from the boxed area in panel A. (Left) Phase-contrast image; (middle) merged images recorded on FRET (green) and YFP (red) channels; (right) pseudocolored ratiometric FRET/YFP images. Scale bar, 3 µm. (C) WT_MA cells. Time traces of fluorescence intensities of two representative cells, recorded on FRET (green lines) and YFP (red lines) channels, are shown. The vertical line represents time of bedaquiline addition. (D) Time traces of FRET/YFP ratios of WT_HA (orange), WT_MA (blue), and WT_LA (black) reporter strains. Ranges of the FRET/YFP ratios corresponding to high-ATP and low-ATP states are highlighted in grey.

    Article Snippet: Aliquots (2 ml) of 7H9 liquid medium containing 2-fold serial dilutions of bedaquiline, isoniazid, rifampin, streptomycin, or ciprofloxacin were inoculated with 2 ml of mid-exponential-phase cultures of M. smegmatis to a final OD600 of ~0.002.

    Techniques: Expressing, Fluorescence, Flow Cytometry

    TLC-autoradiography of FAMEs and MAMEs from M. smegmatis strains overexpressing Mt-KasA, Mt-KasB and Mt-FabH following platensimycin treatment. Platensimycin (0–60 µg/ml) was titred into M. smegmatis cultures at an OD 600 nm of 0.4 prior to labelling with 1 µCi/ml [1,2- 14 C]acetate for 12 h. [ 14 C]-FAMEs and MAMEs were extracted and resolved by TLC. An equivalent aliquot of the resulting solution of FAMEs and MAMEs was subjected to TLC using silica gel plates developed twice in petroleum ether-acetone (95∶5). Autoradiograms were produced by overnight exposure to Kodak X-Omat film to reveal [ 14 C]labeled FAMEs and MAMEs. (A) M. smegmatis pVV16, (B) M. smegmatis pVV16-Mt-KasA, (C) M. smegmatis pVV16-Mt-KasB, and (D) M. smegmatis pVV16-Mt-KasAB.

    Journal: PLoS ONE

    Article Title: Platensimycin Activity against Mycobacterial ?-Ketoacyl-ACP Synthases

    doi: 10.1371/journal.pone.0006306

    Figure Lengend Snippet: TLC-autoradiography of FAMEs and MAMEs from M. smegmatis strains overexpressing Mt-KasA, Mt-KasB and Mt-FabH following platensimycin treatment. Platensimycin (0–60 µg/ml) was titred into M. smegmatis cultures at an OD 600 nm of 0.4 prior to labelling with 1 µCi/ml [1,2- 14 C]acetate for 12 h. [ 14 C]-FAMEs and MAMEs were extracted and resolved by TLC. An equivalent aliquot of the resulting solution of FAMEs and MAMEs was subjected to TLC using silica gel plates developed twice in petroleum ether-acetone (95∶5). Autoradiograms were produced by overnight exposure to Kodak X-Omat film to reveal [ 14 C]labeled FAMEs and MAMEs. (A) M. smegmatis pVV16, (B) M. smegmatis pVV16-Mt-KasA, (C) M. smegmatis pVV16-Mt-KasB, and (D) M. smegmatis pVV16-Mt-KasAB.

    Article Snippet: Platensimycin was added at various concentrations followed by incubation at 37°C for 16 h for M. bovis BCG and 8 h for M. smegmatis at which point 1 µCi/ml [1,2-14 C]acetate (57 mCi/mmol, GE Healthcare, Amersham Bioscience) was added to the cultures.

    Techniques: Thin Layer Chromatography, Autoradiography, Produced, Labeling

    Structures of the major mycolic acids of M. tuberculosis and M. smegmatis .

    Journal: PLoS ONE

    Article Title: Platensimycin Activity against Mycobacterial ?-Ketoacyl-ACP Synthases

    doi: 10.1371/journal.pone.0006306

    Figure Lengend Snippet: Structures of the major mycolic acids of M. tuberculosis and M. smegmatis .

    Article Snippet: Platensimycin was added at various concentrations followed by incubation at 37°C for 16 h for M. bovis BCG and 8 h for M. smegmatis at which point 1 µCi/ml [1,2-14 C]acetate (57 mCi/mmol, GE Healthcare, Amersham Bioscience) was added to the cultures.

    Techniques:

    In vivo effect of platensimycin against M. smegmatis . (A) Clarification of cultures due to clumping and cellular lysis at time point 72 h. (B) Cultures were grown to an OD 600 nm of 0.4 upon which 14 µg/ml of platensimycin was added, samples were take over a 72 h period. Viable counts were calculated as per the methods where the mean CFU per millilitre from three independent experiments was calculated. •, M. smegmatis ; ○, M. smegmatis + platensimycin.

    Journal: PLoS ONE

    Article Title: Platensimycin Activity against Mycobacterial ?-Ketoacyl-ACP Synthases

    doi: 10.1371/journal.pone.0006306

    Figure Lengend Snippet: In vivo effect of platensimycin against M. smegmatis . (A) Clarification of cultures due to clumping and cellular lysis at time point 72 h. (B) Cultures were grown to an OD 600 nm of 0.4 upon which 14 µg/ml of platensimycin was added, samples were take over a 72 h period. Viable counts were calculated as per the methods where the mean CFU per millilitre from three independent experiments was calculated. •, M. smegmatis ; ○, M. smegmatis + platensimycin.

    Article Snippet: Platensimycin was added at various concentrations followed by incubation at 37°C for 16 h for M. bovis BCG and 8 h for M. smegmatis at which point 1 µCi/ml [1,2-14 C]acetate (57 mCi/mmol, GE Healthcare, Amersham Bioscience) was added to the cultures.

    Techniques: In Vivo, Clarification Assay, Lysis

    TLC-autoradiography of M. smegmatis lipid extracts and cell wall bound mycolates following platensimycin treatment. Platensimycin (0, 20, 30, 40 µg/ml) were added to M. smegmatis cultures at an OD 600 nm of 0.4 for 8 h prior to labelling with 1 µCi/ml [1,2- 14 C]acetate for 12 h. (A) 2D-Ag 2+ TLC using silica gel plates developed twice in hexane-ethyl acetate (95∶5) (direction I) then thrice in petroleum ether-diethyl ether (85∶15) (direction Ag II). (B) Cell wall bound mycolate profiles were revealed following two developments in petroleum ether-acetone (95∶5). (C) [ 14 C]-Apolar lipids were extracted and resolved by TLC; direction 1, chloroform-methanol-water (100∶14∶0.8); direction 2, chloroform-acetone-methanol-water (50∶60∶2.5∶3). DAT; diacyltrehalose, GMM; glucose monomycolate, TDM; trehalose dimycolate. Autoradiograms were produced by overnight exposure to film to reveal [ 14 C]-labelled lipids.

    Journal: PLoS ONE

    Article Title: Platensimycin Activity against Mycobacterial ?-Ketoacyl-ACP Synthases

    doi: 10.1371/journal.pone.0006306

    Figure Lengend Snippet: TLC-autoradiography of M. smegmatis lipid extracts and cell wall bound mycolates following platensimycin treatment. Platensimycin (0, 20, 30, 40 µg/ml) were added to M. smegmatis cultures at an OD 600 nm of 0.4 for 8 h prior to labelling with 1 µCi/ml [1,2- 14 C]acetate for 12 h. (A) 2D-Ag 2+ TLC using silica gel plates developed twice in hexane-ethyl acetate (95∶5) (direction I) then thrice in petroleum ether-diethyl ether (85∶15) (direction Ag II). (B) Cell wall bound mycolate profiles were revealed following two developments in petroleum ether-acetone (95∶5). (C) [ 14 C]-Apolar lipids were extracted and resolved by TLC; direction 1, chloroform-methanol-water (100∶14∶0.8); direction 2, chloroform-acetone-methanol-water (50∶60∶2.5∶3). DAT; diacyltrehalose, GMM; glucose monomycolate, TDM; trehalose dimycolate. Autoradiograms were produced by overnight exposure to film to reveal [ 14 C]-labelled lipids.

    Article Snippet: Platensimycin was added at various concentrations followed by incubation at 37°C for 16 h for M. bovis BCG and 8 h for M. smegmatis at which point 1 µCi/ml [1,2-14 C]acetate (57 mCi/mmol, GE Healthcare, Amersham Bioscience) was added to the cultures.

    Techniques: Thin Layer Chromatography, Autoradiography, Produced

    Phosphorylation status and activity of FadD32 produced in mycobacteria. The FadD32 protein was produced either in E. coli (non-phosphorylated, FadD32) or in M. smegmatis (FadD32_myco). A, scatter plots showing maximum velocity V max for lauric acid (C

    Journal: The Journal of Biological Chemistry

    Article Title: Ser/Thr Phosphorylation Regulates the Fatty Acyl-AMP Ligase Activity of FadD32, an Essential Enzyme in Mycolic Acid Biosynthesis *

    doi: 10.1074/jbc.M116.748053

    Figure Lengend Snippet: Phosphorylation status and activity of FadD32 produced in mycobacteria. The FadD32 protein was produced either in E. coli (non-phosphorylated, FadD32) or in M. smegmatis (FadD32_myco). A, scatter plots showing maximum velocity V max for lauric acid (C

    Article Snippet: We first expressed and purified the phosphomimetic FadD32_myco_T552D in M. smegmatis and checked its thermal stability.

    Techniques: Activity Assay, Produced

    Thr-552 is located in an extended loop fully accessible for phosphorylation by STPKs. A, structure-based sequence alignment of FadD32 from M. tuberculosis , M. marinum , M. smegmatis and Corynebacterium glutamicum in the loop region carrying Thr-552. Sequence

    Journal: The Journal of Biological Chemistry

    Article Title: Ser/Thr Phosphorylation Regulates the Fatty Acyl-AMP Ligase Activity of FadD32, an Essential Enzyme in Mycolic Acid Biosynthesis *

    doi: 10.1074/jbc.M116.748053

    Figure Lengend Snippet: Thr-552 is located in an extended loop fully accessible for phosphorylation by STPKs. A, structure-based sequence alignment of FadD32 from M. tuberculosis , M. marinum , M. smegmatis and Corynebacterium glutamicum in the loop region carrying Thr-552. Sequence

    Article Snippet: We first expressed and purified the phosphomimetic FadD32_myco_T552D in M. smegmatis and checked its thermal stability.

    Techniques: Sequencing

    Interpretation of the MBA results with a lawn of fast-growing M. smegmatis . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain

    Journal: Journal of Clinical Microbiology

    Article Title: Use of a Mycobacteriophage-Based Assay for Rapid Assessment of Susceptibilities of Mycobacterium tuberculosis Isolates to Isoniazid and Influence of Resistance Level on Assay Performance

    doi: 10.1128/JCM.44.1.201-205.2006

    Figure Lengend Snippet: Interpretation of the MBA results with a lawn of fast-growing M. smegmatis . Upper left drop, INH-resistant strain incubated without INH (drug-free control); upper right drop, INH-resistant strain incubated with INH; bottom left drop, INH-sensitive strain

    Article Snippet: Phages were detected in 10-μl drops by the formation of plaques on the surface of a lawn of the fast-growing M. smegmatis host (Middlebrook 7H9 agar-OADC-CaCl2 with 108 CFU/ml of M. smegmatis ) after overnight incubation at 37°C.

    Techniques: Incubation

    Delineation of regulatory region . Deletion constructs were generated to segregate promoter and the regulatory regions of IGPr. The column labeled as construct shows configuration of the inserts in different clones used in transformation of M.smegmatis mc2 155 . The numbering is with reference to the translational initiation signal for Rv0167 as +1. The mutation in VPCI591 is shown as a filled triangle, the regions deleted in each clone is indicated by delta symbol. IGPr: 200 bp intergenic region between Rv0166 and Rv0167.

    Journal: BMC Microbiology

    Article Title: Functional analysis of an intergenic non-coding sequence within mce1 operon of M.tuberculosis

    doi: 10.1186/1471-2180-10-128

    Figure Lengend Snippet: Delineation of regulatory region . Deletion constructs were generated to segregate promoter and the regulatory regions of IGPr. The column labeled as construct shows configuration of the inserts in different clones used in transformation of M.smegmatis mc2 155 . The numbering is with reference to the translational initiation signal for Rv0167 as +1. The mutation in VPCI591 is shown as a filled triangle, the regions deleted in each clone is indicated by delta symbol. IGPr: 200 bp intergenic region between Rv0166 and Rv0167.

    Article Snippet: M.smegmatis mc 2 155 (ATCC 700084) was obtained from Dr. Anil Tyagi, South Campus, University of Delhi and Mycobacterium tuberculosis H37Rv were obtained from Central Jalma Institute for leprosy, Agra, India; Mycobacterium tuberculosis VPCI591 is a clinical isolate from Vallabhbhai Patel Chest Institute; Delhi.

    Techniques: Construct, Generated, Labeling, Clone Assay, Transformation Assay, Mutagenesis

    EBP50 expression in RAW264.7 cells after mycobacteria infection. RAW264.7 cells were left uninfected (Con) or infected with M. tuberculosis H37Rv (Mtb), heat-killed M.tuberculosis (D-Mtb) or M. smegmatis mc2-155 (Ms) at the MOIs of 10 for 24 h. mRNA expression levels of EBP50 were measured by real-time PCR (A) . EBP50 protein levels were measured by Western blotting. β-actin was used as a loading reference (B) . Band density of the specific protein was analyzed with Quantity One image software, and the results were expressed as average density to β-actin (C) . Data are represented as mean ± SEM of at least three independent experiments. * P

    Journal: Scientific Reports

    Article Title: EBP50 induces apoptosis in macrophages by upregulating nitric oxide production to eliminate intracellular Mycobacterium tuberculosis

    doi: 10.1038/srep18961

    Figure Lengend Snippet: EBP50 expression in RAW264.7 cells after mycobacteria infection. RAW264.7 cells were left uninfected (Con) or infected with M. tuberculosis H37Rv (Mtb), heat-killed M.tuberculosis (D-Mtb) or M. smegmatis mc2-155 (Ms) at the MOIs of 10 for 24 h. mRNA expression levels of EBP50 were measured by real-time PCR (A) . EBP50 protein levels were measured by Western blotting. β-actin was used as a loading reference (B) . Band density of the specific protein was analyzed with Quantity One image software, and the results were expressed as average density to β-actin (C) . Data are represented as mean ± SEM of at least three independent experiments. * P

    Article Snippet: Bacterial culture M. smegmatis mc2 -155 (ATCC 700084), M. tuberculosis H37Rv (ATCC 27294), and M. tuberculosis -GFP were grown to early mid-log phase in Middlebrook 7H9 broth with or without hygromycin B, supplemented with 10% ADC and 0.5% glycerol at 37 °C.

    Techniques: Expressing, Infection, Mass Spectrometry, Real-time Polymerase Chain Reaction, Western Blot, Software

    EBP50 knockdown decreases the levels of iNOS, NO and apoptosis ratios of RAW264.7 cells following mycobacteria infection. RAW264.7 cells were left untreated (Con) or transduced with LV-shEBP50 or LV-shCon for 72 h. The expression levels of EBP50 were determined by Western blotting (A) . RAW264.7 cells were left untreated (Con) or transduced with LV-shEBP50 or LV-shCon for 72 h, then infected with M. smegmatis mc2-155 (Ms) at the MOIs of 10 for 24 h. The amount of iNOS was detected by Western blotting. β-actin was used as a loading reference (B) . The production of NO was measured by Griess reagent (C) and the apoptosis incidence was measured by FCM (D) . Data are represented as mean ± SEM of at least three independent experiments. ** P

    Journal: Scientific Reports

    Article Title: EBP50 induces apoptosis in macrophages by upregulating nitric oxide production to eliminate intracellular Mycobacterium tuberculosis

    doi: 10.1038/srep18961

    Figure Lengend Snippet: EBP50 knockdown decreases the levels of iNOS, NO and apoptosis ratios of RAW264.7 cells following mycobacteria infection. RAW264.7 cells were left untreated (Con) or transduced with LV-shEBP50 or LV-shCon for 72 h. The expression levels of EBP50 were determined by Western blotting (A) . RAW264.7 cells were left untreated (Con) or transduced with LV-shEBP50 or LV-shCon for 72 h, then infected with M. smegmatis mc2-155 (Ms) at the MOIs of 10 for 24 h. The amount of iNOS was detected by Western blotting. β-actin was used as a loading reference (B) . The production of NO was measured by Griess reagent (C) and the apoptosis incidence was measured by FCM (D) . Data are represented as mean ± SEM of at least three independent experiments. ** P

    Article Snippet: Bacterial culture M. smegmatis mc2 -155 (ATCC 700084), M. tuberculosis H37Rv (ATCC 27294), and M. tuberculosis -GFP were grown to early mid-log phase in Middlebrook 7H9 broth with or without hygromycin B, supplemented with 10% ADC and 0.5% glycerol at 37 °C.

    Techniques: Infection, Transduction, Expressing, Western Blot, Mass Spectrometry

    Photodynamic inactivation of Mycobacterium smegmatis mc 2 155 ATCC-700084 as a function of DIMPy-BODIPY concentration. Displayed is the % survival of the dark control (♦) and the light treated samples ( ■ ). Illumination and assay conditions were as in Figure 3 .

    Journal: Molecules

    Article Title: Antiviral, Antifungal and Antibacterial Activities of a BODIPY-Based Photosensitizer

    doi: 10.3390/molecules200610604

    Figure Lengend Snippet: Photodynamic inactivation of Mycobacterium smegmatis mc 2 155 ATCC-700084 as a function of DIMPy-BODIPY concentration. Displayed is the % survival of the dark control (♦) and the light treated samples ( ■ ). Illumination and assay conditions were as in Figure 3 .

    Article Snippet: The taxonomically Gram-positive bacterium M. smegmatis strain ATCC-700084, shown separately in , exhibited ~4 log units inactivation at 1 μM (p < 0.001), ~3.3 log units at 0.5 μM (p < 0.001), and 95% inactivation (~2 log units, p < 0.001) at 0.25 μM.

    Techniques: Concentration Assay

    Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. smegmatis mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and

    Journal:

    Article Title: Novel Streptomycin Resistance Gene from Mycobacterium fortuitum ▿

    doi: 10.1128/AAC.00223-06

    Figure Lengend Snippet: Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. smegmatis mc 2 155. orfA , orfB , and orfE are found in the loci of both M. fortuitum and M. smegmatis ; orfC and

    Article Snippet: For this, we first obtained crude extracts from M. smegmatis strains by sonication (Bioruptor; Cosmo Bio Co., Ltd.).

    Techniques: Clone Assay