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    New England Biolabs m mulv reverse transcriptase new england biolabs
    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C <t>(M−MuLV</t> reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart <t>RTx</t> Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p
    M Mulv Reverse Transcriptase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs moloney murine leukemia virus reverse transcriptase
    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C <t>(M−MuLV</t> reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart <t>RTx</t> Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/New England Biolabs
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    New England Biolabs m mulv moloney murine leukemia virus taq reverse transcriptase
    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C <t>(M−MuLV</t> reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart <t>RTx</t> Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p
    M Mulv Moloney Murine Leukemia Virus Taq Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv moloney murine leukemia virus taq reverse transcriptase/product/New England Biolabs
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    Price from $9.99 to $1999.99
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    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Journal: Journal of Advanced Research

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries

    doi: 10.1016/j.jare.2019.12.005

    Figure Lengend Snippet: Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Article Snippet: M−MuLV reverse transcriptase, ProtoScript® II Reverse Transcriptase, WarmStart RTx Reverse Transcriptase, RNase H, DNase I, Exonuclease I, Murine RNase Inhibitor, NEBNext® rRNA Depletion Kit (Human/Mouse/Rat), and NEBNext Ultra Directional RNA Library Prep Kit for Illumina were purchased from New England Biolabs (NEB, Ipswich, MA).

    Techniques: Expressing