m-280 streptavidin coated dynabeads Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dynabeads m 280 streptavidin
    Dynabeads M 280 Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3076 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads m 280 streptavidin/product/Thermo Fisher
    Average 99 stars, based on 3076 article reviews
    Price from $9.99 to $1999.99
    dynabeads m 280 streptavidin - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    78
    Thermo Fisher avidin coated m 280 dynabeads
    Avidin Coated M 280 Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin coated m 280 dynabeads/product/Thermo Fisher
    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    avidin coated m 280 dynabeads - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    99
    Thermo Fisher streptavidin coated beads
    Streptavidin Coated Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated beads/product/Thermo Fisher
    Average 99 stars, based on 592 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated beads - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher streptavidin coated dynabeads
    Streptavidin Coated Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated dynabeads/product/Thermo Fisher
    Average 99 stars, based on 575 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated dynabeads - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher streptavidin coated magnetic beads
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Streptavidin Coated Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 3949 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated magnetic beads - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    82
    Thermo Fisher streptavidin coated polystyrene beads
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Streptavidin Coated Polystyrene Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated polystyrene beads/product/Thermo Fisher
    Average 82 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated polystyrene beads - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    78
    Thermo Fisher stretavidin coated magnetic beads mbs
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Stretavidin Coated Magnetic Beads Mbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stretavidin coated magnetic beads mbs/product/Thermo Fisher
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    stretavidin coated magnetic beads mbs - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    89
    Thermo Fisher streptadivin coated dynabeads
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Streptadivin Coated Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptadivin coated dynabeads/product/Thermo Fisher
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    streptadivin coated dynabeads - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    81
    Thermo Fisher avidin coated magnetic bead suspension
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Avidin Coated Magnetic Bead Suspension, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin coated magnetic bead suspension/product/Thermo Fisher
    Average 81 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    avidin coated magnetic bead suspension - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    78
    Thermo Fisher streptavidin coated sepharose dynabeads m280 streptavidin
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Streptavidin Coated Sepharose Dynabeads M280 Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated sepharose dynabeads m280 streptavidin/product/Thermo Fisher
    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated sepharose dynabeads m280 streptavidin - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    89
    Thermo Fisher streptavidin coated sepharose beads
    Scheme of the DNA binding and release assay. (A) <t>Streptavidin-coated</t> magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.
    Streptavidin Coated Sepharose Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated sepharose beads/product/Thermo Fisher
    Average 89 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated sepharose beads - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    Image Search Results


    Scheme of the DNA binding and release assay. (A) Streptavidin-coated magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.

    Journal: Molecular and Cellular Biology

    Article Title: An ATP/ADP-Dependent Molecular Switch Regulates the Stability of p53-DNA Complexes

    doi:

    Figure Lengend Snippet: Scheme of the DNA binding and release assay. (A) Streptavidin-coated magnetic beads were used to bind biotinylated oligonucleotide targets. After washing, the DNA-bound beads were incubated with p53. Bound p53-DNA complexes on the beads were washed (to remove nonbound p53) and incubated in fresh buffer, with or without nucleotides, for 10 min at 37°C (or longer for time course experiments). Subsequently the released p53 (in solution) and DNA-bound p53 were analyzed by scintillation counting (for radiolabelled p53) and PAGE (15% polyacrylamide) followed by immunoblotting or autoradiography. (B) Binding of 35 S-labelled p53 to magnetic beads coated with different oligonucleotides (detailed in Materials and Methods). An aliquot of beads without DNA was included as negative control (beads lane). Other lanes: CON-DNA, beads prebound with a consensus sequence-specific p53 DNA binding site; L-DNA, dsDNA containing triple insertion-deletion lesion; NL-DNA, dsDNA with blunt ends (the bottom strand of NL-DNA alone was used as ssDNA target) (see Materials and Methods). RNA was obtained by in vitro transcription from p53 cDNA under the T7 promoter by using biotinylated UTP. The faster-migrating band (below p53) represents a truncated p53 product that is routinely observed for in vitro-translated p53. wt, wild type.

    Article Snippet: Streptavidin-coated magnetic beads (M-280 Dynabeads; Dynal) were used to harvest biotinylated DNA-protein complexes. dsDNA or ssDNA oligonucleotides were bound to the beads (typically 75 pmol of oligonucleotide per 40 μl of beads for each reaction) in Tris-EDTA (pH 7.5)–1 M NaCl (TE-NaCl) for 15 min at 20°C and washed twice with 400 μl of TE-NaCl and twice with 400 μl of DNA binding buffer (20 mM Tris-HCl [pH 7.5], 100 mM NaCl, 0.1% NP-40, 10% glycerol, 5 mM DTT) to remove nonbound DNA.

    Techniques: Binding Assay, Release Assay, Magnetic Beads, Incubation, Polyacrylamide Gel Electrophoresis, Autoradiography, Negative Control, Sequencing, In Vitro